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All Journal AL KAUNIYAH El-Hayah : Jurnal Biologi SAINSTIS Padjadjaran Journal of Dentistry Jurnal Biodjati Jurnal Pengabdian Pada Masyarakat Padjadjaran Journal of Dental Researchers and Students INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Journal of Applied Health Management and Technology Jurnal Riset Biologi dan Aplikasinya Jurnal Abdimas Bina Bangsa Jurnal Kesehatan Gigi dan Mulut (JKGM) Jurnal Terapi Gigi dan Mulut Media Penelitian dan Pengembangan Kesehatan
Ulfah Utami
Universitas Islam Negeri Maulana Malik Ibrahim Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : El-Hayah : Jurnal Biologi

 1 
Potency of Endophyte Microbes as an Antagonistic Agent for Colletotrichum capsici Causes Anthracnose Disease in Cayenne pepper (Capsicum frustescens) Afifah, Zahroul; Utami, Ulfah
El-Hayah : Jurnal Biologi Vol 6, No 3 (2017): EL-HAYAH (VOL 6, NO 3,September 2017)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v6i3.5804

Abstract

Background: An anthracnose disease caused by pathogenic fungal Colletotrichum capsici has been attacking the cayenne plants either harvested or has not been harvested. This disease must be handled appropriately and quickly because it can reduce the production of chili up to 90%. Recently, anthracnose disease prevention still use chemical fungicide that if applied for long time will cause new impact for environment. Objective: Trichoderma and Bacillus cereus endophytes may be used as antagonistic agents for C. capsici pathogens because they have various antibiotic compounds. Methods: This research uses experimental method. The stages of this study include sterilization of tools and materials, preparation of culture media of fungal and bacteria, rejuvenation of endophytic microbe culture Trichoderma sp. and Bacillus cereus, rejuvenation of  C. capsici pathogen, antagonistic test in vitro using dual culture method. Result and conclusion: The results of in vitro antagonistic tests showed that inhibition percentage of Trichoderma treatment (96%) and combination treatment Trichoderma and B. cereus (97%) is not significantly different. While in B. cereus treatment (11, 88%) significantly different with all of treatments. Endophytes are shown by its dominating growth in petri dishes than C. capsici pathogen or B. cereus endophytes. Furthermore,for endophytes Trichoderma sp continued on in vivo test because it was most effective.The result for incubation period is 3 days after inoculation compared with negative control 2 days. For disease incidence 100%, and for disease intensity that is 61,25% compared with negative control equal to 88,75%.
The Effect of Addition Mn2+ Metal Ions and Incubation Time to Bacillus cereus Cellulase Enzyme Activity from Endophytic Bacteria of Curcuma Rhizome (Curcuma zanthorrizha Roxb.) Sulistyantini, Cahyaning; Utami, Ulfah
El-Hayah : Jurnal Biologi Vol 8, No 2 (2021): EL-HAYAH (VOL 8, NO 2 MARET 2021)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v8i2.12463

Abstract

Cellulase is one of the most widely used enzymes in the industrial world and wastes decomposition process. Bacillus cereus is one of the bacteria that can produce cellulase enzymes that can hydrolyze cellulose to glucose. The addition of cofactors and incubation time can help determine the optimum conditions needed by cellulase enzymes to work optimally. This study aims to determine the effect of adding Mn2+ metal ions and incubation time to the activity of cellulase enzymes from Bacillus cereus endophytic bacteria. This research is experimentally used a Completely Randomized Design (CRD) factorial design with two factor treatments and 3 times repetitions. The first factor is variation of addition Mn2+ metal ions which are 5 mM, 10 mM, and 15 mM, the second is variation of incubation time which are 1 hour, 2 hours, 3 hours, 4 hours, and 5 hours. The data were analyzed using Analysis Of Variance (ANOVA) and if the data significantly affected the parameter, then it would be continued by Duncan Multiple Range Test (DMRT) with the fault level of 5%. The result showed that the interaction of addition Mn2+ metal ions and incubation time affected the cellulase enzyme activity of Bacillus cereus from endophytic bacteria. The highest cellulase enzyme activity obtained from interaction addition Mn2+ metal 10 mM and incubation time 3 hours with an activity 0,335 U/mL, while the lowest cellulase enzyme activity obtained from interaction addition Mn2+ metal 15 mM and incubation time 5 hours with an activity of 0,073 U/mL
Co-Authors Afifah, Zahroul Ahdiansyah, Balqis Shafa' Ahmad Barizi Anggeria Oktavisa Denta Aprilianty, Windy Marsella Arifin, Naufal Aqila Azizah, Dita Nur Azizatur Rahmah Chaerudin, Denden Ridwan Dina Istia'nah ELDARITA, ELDARITA Elfiyatinnufus, Rifqiyah Fitriyah Fitriyah Fitriyani, Annisa Fuzzi Handayani, Ratna Dwi Heriyanto, Yonan Husna, Naila Fatihatul Istia'nah, Dina Izzatinnisa' Izzatinnisa' Izzatinnisa', Izzatinnisa' Kamila, Syadza Afifah Laela, Dewi Sodja Laut, Deru Marah Liliek Harianie Lilik Hariani Marah Laut, Deru Megananda Hiranya Putri Muh Amin, dwi aisyah Muhammad Asmuni Hasyim Muhammad Riefki Pratama Mujahidin Ahmad Neneng Nurjanah Nur Kusmiyati Nurnaningsih, Hera Nurul Fatikhah, Nurul Nuzulul Zahroh Praptiwi, Yanni Hendriani Praptiwi, Yenni Hendriani Pratama, Muhammad Riefki Prilya Dewi Fitriasari Retno Setyaningrum Rodian, Disti Rahayu Sari, Cici Rafita Siti Fatimah Sri Mulyanti Sulistyantini, Cahyaning Supriyanto, Irwan Widyastuti , Tri Yuni Puspitasari Yuti Malinda