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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Silvikultur Tropika (JST) Jurnal Hortikultura Pelita Perkebunan Planta Tropika Journal of Tropical Crop Science
Ade Wachjar
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti Kampus IPB Darmaga, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Energy Environmental Science Social Sciences

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Characterization of Arbuscular Mychorrizal Fungus from Sugar Palm (Arenga pinnata (Wurmb) Merr.)West Java and Banten KARAKTERISASI FUNGI MIKORIZA ARBUSKULA PADA RHIZOSFER AREN (Arenga pinnata (Wrmb) Merr.) DARI JAWA BARAT DAN BANTEN Moh Ega Elman Miska; Ahmad Junaedi; Ade Wachjar; Irdika Mansur
Jurnal Silvikultur Tropika Vol. 7 No. 1 (2016): Jurnal Silvikultur Tropika
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.7.1.%p

Abstract

The diversity of AMF has been recorded as much as 250 types which associated with certain plants and inhabit the areas of tropical, temperate, and even the arctic. This research aims to find out the diversity of Arbuscular Mycorrhizal Fungus under the sugar palm tree stands in different locations. Soil were sampled from the rhizosphere of sugar palm in three subdistricts i.e. Cianjur, Sukabumi, and Lebak. These samples were processed directly by wet sieving and sentrifugation method to separate the AMF spore., These spores were then identified immediately. The results showed there were four genera and 14 morpho types AMF spores, which consists of: seven types of Glomus sp.; five types of Acaulospora sp.; one Scutellospora sp.; and one Gigaspora sp.Key words : AMF, Banten, diversity, sugar palm, West Java.
Identification of Molecular Marker Based on MYB Transcription Factor for the Selection of Indonesian Fine Cacao (Theobroma cacao L.) Lukita Devy; Indah Anita-Sari; Tengku Imam Saputra; Agung Wahyu Susilo; Ade Wachjar; . Sobir
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 34 No 2 (2018)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v34i2.314

Abstract

Indonesia is the third largest cacao (Theobroma cacao L.) producer in the world and also well-known for its fine cacao varieties (Java fine-flavor cacao). Indonesian fine cacao breeding program will be accelerated by early detection of its specific trait through the use of molecular marker. One of the traits thatcould differentiate fine and bulk cacao, in this case Criollo and Forastero, respectively, is the pod color. Previous research reported that MYB transcription factor gene regulated cacao pod color and was able to differentiate Criollo from Forastero. The gene involved in the control of plant-specific processes including primary and secondary metabolism, cell fate and identity, developmental processes and responses to biotic and abiotic stresses. This research aimed to identify the diversity of Indonesian fine and bulk cacao based on MYB nucleotide sequence fragments. Identification of the MYB nucleotide sequence was conducted by DNA isolation from cacao leaves and specific primer design based on two cacao MYB transcription factor ene accessions. These primers were used to evaluate the diversity of three Indonesian fine cacao (DRC 16, PNT 16, and ICCRI 01) and two bulk cacao (PA 191 and ICCRI 03) clones. The cluster analysis showed that this specific primer is similar to other MYB gene accessions in Malvaceae family (Theobroma, Herrania, Gossypium). It is also able to differentiate bulk and fine cacao in accordance to their pedigree. The primer developed in this study could be used for further analysis of Indonesian fine cacao molecular marker.
Growth Response of Seedlings of Four Robusta Coffee (Coffea canephora Pierre. Ex. A. Froehner) Clones to Drought Stress Novie Pranata Erdiansyah; Ade Wachjar; Eko Sulistyono; Supijatno Supijatno
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 35 No 1 (2019)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v35i1.344

Abstract

Drought is one of the limiting factors for the growth and yield of coffee plants. Drought due to long dry season has caused many losses for coffee plantations. This study aimed to evaluate the response of four Robusta coffee clones to drought stress at seedling stage and determine the best clone with high tolerance to drought. The study was conducted in Indonesian Coffee and Cocoa Reseach Institute (ICCRI) Jember, East Java in November 2018–Mei 2019. The experimental design used a Randomized Block Design (RCBD) with two factors. The first factor has consisted of five levels drought through providing volume watering based coefficient and evaporation value of free water surface (Eo), namely: 0.5 Eo; 1.0 Eo; 1.5 Eo; 2.0 Eo; 2.5 Eo (control). The second factor has consisted of four Robusta coffee clones, namely: BP 409 (drought tolerant clone); BP 308; BP 939 and BP 358 (vulnerable to drought stres). The result showed that reduction in the volume of watering from 2.5 Eo until 0.5 Eo causes drought, reduce coffee growth linearly. Clone BP 409 and BP 939 had better tolerance of drought stress compared with BP 308 and BP 358. The anatomical adaptation of leaves of BP 409 to drought stress was by thickening of wax layer and palisade tissue. BP 939 thickened its leaves due to drought stress as a mechanism of adaptation to such condition.
Determination of Criteria and Drought Tolerance of Prope-Legitimate Cocoa Seed Through Polyethylene Glycol Induction Maera Zasari; Agung Wahyu Susuilo; Ade Wachjar; Sudarsono Sudarsono
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 36 No 2 (2020)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v36i2.435

Abstract

The availability of drought tolerant rootstock becomes one of solution to obtain tolerant cocoa clone at drought condition. The production of rootstock is easier and faster by using prope-legitimate seeds as plant materials. Seed germination selection phase can accelerate the obtainment of tolerant cocoa rootstocks. The tolerance trait of prope-legitimate seeds can be determined by seed germination on media induced by polyethylene glycol (PEG) solution. To determine the tolerant seeds, it is effective by using a specific character as selection criteria. This study aimed to obtain criteria and selection character to determine droughttolerant characteristic of prope-legitimate seed through PEG 6000 induction in the germination phase. The research was conducted at the Agronomy Laboratoryof the Indonesian Coffee and Cocoa Research Institute, Jember, East Java, Indonesia, used split plot design with five replications as experimental design. The main plot was solution of 0% and 6% PEG 6000, while the subplot were prope-legitimate seeds of 13 cocoa clones. The result showed that germination ability and sprout fresh weight were suitable as selection criteria to determine tolerant characteristic of prope-legitimate seeds to drought stress. Based on the value of tress susceptible index and clustering analysis, the tested prope-legitimate seeds were divided into three groups. The group of tolerant genotype consisted of Sca 06, while the group of medium tolerant were ICCRI 03, Sulawesi 02, KW 641, TSH 858, KEE 02, Sulawesi 03, and KW 617. The susceptible group consisted of six genotypes, namely ICS 60, KKM 22, KW 516, Sulawesi 01, and MCC 02.
Physio-Biochemical Characteristics of Prope Legitimate Seedlings of 13 Cocoa Clones Under Drought Stress Zasari, Maera; Wachjar, Ade; Susilo, Agung Wahyu; Sudarsono, Sudarsono
PLANTA TROPIKA Vol 11, No 2 (2023)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v11i2.14315

Abstract

The tolerant seedlings are determined by their physiological and biochemical responses. This study aimed to determine stomatal density, relative water content, and proline in prope legitimate seedlings under drought stress. The research was carried out at the Indonesian Coffee and Cocoa Research Center in July 2018 – February 2019, arranged in a randomized split-plot experimental design with six replications. The main plot was soil moisture content, and the sub-plot was the cocoa prope legitimate seedlings. The differences of mean values were tested using analysis of variance, followed by the DMRT and T-student test at 5 % level, analysis of the relative decrease in stomatal density and relative water content, proline content ratio, and the dendrogram analysis. The results showed that the cocoa seedlings under drought experienced significant changes in relative water and proline content but not stomatal density. Drought decreased in the relative water content of 50% and increased proline content in the seedlings. The relative water and proline content divided the prope legitimate seedlings into two groups. The prope legitimate seedlings from KW 516, KW 641, Scavina 06, KKM 22, KW 617, and ICCRI 03 clones were drought-tolerant.
Water Footprint Analysis of Different Techniques of Cocoa Propagation Santosa, Edi; Supijatno, Supijatno; Wachjar, Ade; Rohman, Fadil; Abdoellah, Soetanto
Journal of Tropical Crop Science Vol. 10 No. 03 (2023): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.10.03.153-165

Abstract

The nursery phase plays a crucial role in rejuvenating cocoa plantations as it significantly impacts the quality and productivity of the mature trees in the field. However, despite its significance, there remains a lack of understanding regarding its contribution to the water footprint (WF) in cocoa production. This study aims to assess the WF of various propagation techniques to promote sustainable nursery practices. Data on nurseries were collected at the Indonesian Coffee and Cocoa Research Institute in Jember, East Java, Indonesia, from June 2017 to January 2018. The results revealed that propagation accounted for a total WF ranging from 74.28 to 319.41 m3.ha-1 of established cocoa trees, with an average of 186.68 m3. This total WF consisted of 9.02 to 12.89 m3 (7.69%) attributed to seed production and 61.39 to 283.34 m3 (92.30%) attributed to the nursery phase. Among the different nursery techniques studied, the production of true seedlings exhibited the lowest WF, followed by side grafting. To optimize cocoa rejuvenation and minimize WF, it is crucial to carefully select the appropriate nursery technique. Further evaluation is necessary to explore the potential benefits of implementing precision irrigation techniques to reduce WF during the nursery phase. By focusing on sustainable nursery practices, we can enhance the overall sustainability of cocoa production.
Optimasi Dosis Pupuk Majemuk NPK dan Kalsium pada Bibit Kelapa Sawit (Elaeis guineensis Jacq.) di Pembibitan Utama Ramadhaini, Rizki Fauziah; ., Sudradjat; Wachjar, Ade
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 1 (2014): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (346.856 KB) | DOI: 10.24831/jai.v42i1.8151

Abstract

ABSTRACTThis research was aimed to evaluate the rates of NPK and calcium fertilizers for the growth of oil palm seedling at main nursery. It was conducted in IPB Experimental Station, Cikabayan, Darmaga, Bogor from December 2011 to November 2012. The two factors, NPK and calcium, were arranged in a randomized block design with three replications. The rates of NPK fertilizer (15-15-15) were 0, 115, 230 and 460 g seedling-1. The rates of calcium fertilizer were 0, 5, 10 and 20 g seedling-1. There was no interaction effect observed between NPK and calcium fertilizer. NPK fertilizer, however had the significant quadratic effect on plant height, leaf number, stem diameter and chlorophyll content. Based on morphology variables, recommended optimum rate of NPK 15-15-15 fertilizer was 333 g seedling-1 for eight months. Thus, NPK rates were 7.00, 7.00, 19.45, 59.25, 66.3, 61.55, 58.97 and 54.16 g seedling-1 from first to eight month respectively. The optimum rate of calcium fertilizer was not determined in this experiment.Keywords: NPK 15-15-15, nutrient, optimum rates, recommendation
Pertumbuhan Mucuna bracteata DC. pada Berbagai Waktu Inokulasi dan Dosis Inokulan Laksono, Purwanti Budi; Wachjar, Ade; Supijatno, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 1 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (503.495 KB) | DOI: 10.24831/jai.v44i1.12510

Abstract

ABSTRACTMucuna bracteata DC. is a legume cover crop to prevent erosion and to suppress weed growth. M. bracteata can fix N2 from the air with the help of Rhizobium. M. bracteata get benefit from the symbiosis in the form of increased nodule weight, shoot dry weight, and leaf nitrogen content when the Rhizobium population in the soil is optimal. Application of inoculant is one method to increase the Rhizobium population in the soil. This study aimed to analyze the effects of inoculation times and inoculant rates on M. bracteata growth. The research was conducted in Boyolali, Central Jawa, from February to August 2014. The inoculant contained Bradyrhizobium japonicum and Aeromonas punctata. This experiment was arranged in a randomized block design with two factors and three replications. The first factor was two inoculation times, i.e at the nursery when the seedlings were 2 weeks old and in the field when the seedlings reached 5 weeks old. The second factor was 5 inoculant rates (0, 2, 4, 6, and 8 g per plant). The results showed that inoculation on 5-week-old seedlings was the best result compared to the 2-week-old seedlings. The recommended inoculant rate for Mucuna bracteata was 6.43 g per plant. Keywords: legume cover crop, nitrogenase activity, nodule, nutrient content, runner
Tree-based Water Footprint Assessment on Established Oil Palm Plantation in North Sumatera, Indonesia Santosa, Edi; Stefano, Indra Mario; Gani Tarigan, Abdul Gani; Wachjar, Ade; Zaman, Sofyan; Agusta, and Herdhata
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 46 No. 1 (2018): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (421.473 KB) | DOI: 10.24831/jai.v46i1.13665

Abstract

ABSTRACTIn a long life cycle of oil palm plantation, sustainable water management is mandatory because irrigation is rarely applied. In order to develop water management for sustainable palm oil production, tree-based water footprint of well-established oil palm plantation was assessed. Field data were collected from February to June 2016 in Dolok Ilir managed by PTPN IV, North Sumatera, Indonesia. Additional data were obtained from interviews on the site and the surrounding estates, reports and references. Results showed that water footprint (WF) for production of fresh fruit bunch (FFB) was 510.69 m3 tonne-1 and crude palm oil (CPO) was 517.79 m3 tonne-1. Green, blue and grey water contributed 94.78%, 0.71% and 4.50% in FFB, and 93.48%, 1.66% and 4.85% in CPO productions, respectively. All green WF was calculated basen on actual value of tree evapotranspiration, therefore, the value was mostly lower than other researchs. Low amount of blue water indicates that the oil palm tree in North Sumatera extracts low amount of ground water. On the other hand, grey water for pollution dilution of fertilizers, pesticides and herbicides were high, i.e., 15.15 m3, 4.77 m3, 3.07 m3 tonne-1 FFB, respectively. It implies that reduction of grey water should be implemented in the near future through precission farming.Keywords: CPO, Elaeis guineensis, precission farming, sustainable production, water footprint
Respon Pertumbuhan Tanaman Cengkeh (Syzygium aromaticum (L.) Merr. Perr.) dengan Aplikasi Pupuk Organik-Anorganik dan Triakontanol Launuru, Syahdin; Wachjar, Ade; Kurniawati, Ani
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 47 No. 3 (2019): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (514.365 KB) | DOI: 10.24831/jai.v47i3.26017

Abstract

Application of right dosage of fertilizer and concentration of plant growth regulator are supporting factor of clove growth. This study was aimed to obtain the best combination of organic-inorganic fertilizer, the best triacontanol concentration and both interactions for increasing clove growth. This study used a Randomized Complete Block Design (RCBD) with two factors. The first factor was the organic-inorganic fertilizer dosage package that consisted of 6 levels, i.e no fertilizer, 100% recommended dosage of inorganic fertilizer (r.d.i.f), 25% recommended dosage of organic fertilizer (r.d.o.f) + 75% r.d.i.f, 50% r.d.o.f + 50% r.d.i.f, 75% r.d.o.f + 25% r.d.i.f, 100% r.d.o.f. The second factor was the concentration of triacontanol that consisted of 3 levels, i.e no triacontanol, 2 mg L-1 and 4 mg L-1. The results showed that the application of 25% r.d.o.f + 75% r.d.i.f produced the best clove growth, but it was not significantly different from the combination treatment of the two other types of fertilizer as indicated by the greatest improvement on stem diameter, branches number, leaf area, chlorophyll a, chlorophyll b, total chlorophyll and carotenoids. Clove plants did not respond to the application of triacontanol at a concentration of 2 mg L-1, but gave a negative response to the application of triacontanol 4 mg L-1 on the plant height and leaf area variables. The interaction of the two factors did not have significant effects on all variables. Keywords: chlorophyll, morphology, nutrient dynamics, physiology, vegetative growth
Co-Authors . Sobir . Supriadi . Supriadi, . Abdoellah, Soetanto Agung Wahyu Susilo Agung Wahyu Susilo Agung Wahyu Susuilo AHMAD JUNAEDI Aiyen Tjoa Andi Besse Poleuleng Ani Kurniawati Anita Darwis Anugrah, Pangeran Teguh Atikah, Nurhati Delima Ragil Kartika Dianto, Fajar Direja, Wahyu Angga Edi Santosa Efendi, Darda Eko Sulistyono Gani Tarigan, Abdul Gani Hariyadi, Hariyadi Herdhata Agusta Ilyas Marzuki Indah Anita-Sari Irdika Mansur Kartika, Delima Ragil Krisantini Kusnendi, Faizal Shofwan Laksono, Purwanti Budi Launuru, Syahdin Lukita Devy Maera Zasari Mahulette, Asri Subkhan Miska, Moch. Ega Elman Nindita, Anggi Novie Pranata Erdiansyah Odit Ferry Kurniadinata Rizki Fauziah Ramadhaini Rizkiana Anggayuhlin Rizkiana Anggayuhlin Roedhy Poerwanto Rohmah, Naelatur Rohman, Fadil Saiful Akhyar Lubis Sari, Allice Fajri Chynthia Sianturi, Vinsensia Febrina Siregar, Midian Romeo Stefano, Indra Mario Sudarsono Sudarsono Sudarsono Sudirman Yahya Sudradjat , Sudradjat Sudrajat Sugeng Heri Suseno Suhartanto, Muhammad Rahmad Sulistianingrum, Ratih Supijatno Tengku Imam Saputra Ugroseno, Rene Vinsensia Febrina Sianturi Wahyu Angga Direja Zaman, Sofyan