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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Silvikultur Tropika (JST) Jurnal Hortikultura Pelita Perkebunan Planta Tropika Journal of Tropical Crop Science
Ade Wachjar
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti Kampus IPB Darmaga, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Energy Environmental Science Social Sciences

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Characterization of Arbuscular Mychorrizal Fungus from Sugar Palm (Arenga pinnata (Wurmb) Merr.)West Java and Banten KARAKTERISASI FUNGI MIKORIZA ARBUSKULA PADA RHIZOSFER AREN (Arenga pinnata (Wrmb) Merr.) DARI JAWA BARAT DAN BANTEN Moh Ega Elman Miska; Ahmad Junaedi; Ade Wachjar; Irdika Mansur
Jurnal Silvikultur Tropika Vol. 7 No. 1 (2016): Jurnal Silvikultur Tropika
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.7.1.%p

Abstract

The diversity of AMF has been recorded as much as 250 types which associated with certain plants and inhabit the areas of tropical, temperate, and even the arctic. This research aims to find out the diversity of Arbuscular Mycorrhizal Fungus under the sugar palm tree stands in different locations. Soil were sampled from the rhizosphere of sugar palm in three subdistricts i.e. Cianjur, Sukabumi, and Lebak. These samples were processed directly by wet sieving and sentrifugation method to separate the AMF spore., These spores were then identified immediately. The results showed there were four genera and 14 morpho types AMF spores, which consists of: seven types of Glomus sp.; five types of Acaulospora sp.; one Scutellospora sp.; and one Gigaspora sp.Key words : AMF, Banten, diversity, sugar palm, West Java.
Identification of Molecular Marker Based on MYB Transcription Factor for the Selection of Indonesian Fine Cacao (Theobroma cacao L.) Lukita Devy; Indah Anita-Sari; Tengku Imam Saputra; Agung Wahyu Susilo; Ade Wachjar; . Sobir
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 34 No 2 (2018)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v34i2.314

Abstract

Indonesia is the third largest cacao (Theobroma cacao L.) producer in the world and also well-known for its fine cacao varieties (Java fine-flavor cacao). Indonesian fine cacao breeding program will be accelerated by early detection of its specific trait through the use of molecular marker. One of the traits thatcould differentiate fine and bulk cacao, in this case Criollo and Forastero, respectively, is the pod color. Previous research reported that MYB transcription factor gene regulated cacao pod color and was able to differentiate Criollo from Forastero. The gene involved in the control of plant-specific processes including primary and secondary metabolism, cell fate and identity, developmental processes and responses to biotic and abiotic stresses. This research aimed to identify the diversity of Indonesian fine and bulk cacao based on MYB nucleotide sequence fragments. Identification of the MYB nucleotide sequence was conducted by DNA isolation from cacao leaves and specific primer design based on two cacao MYB transcription factor ene accessions. These primers were used to evaluate the diversity of three Indonesian fine cacao (DRC 16, PNT 16, and ICCRI 01) and two bulk cacao (PA 191 and ICCRI 03) clones. The cluster analysis showed that this specific primer is similar to other MYB gene accessions in Malvaceae family (Theobroma, Herrania, Gossypium). It is also able to differentiate bulk and fine cacao in accordance to their pedigree. The primer developed in this study could be used for further analysis of Indonesian fine cacao molecular marker.
Growth Response of Seedlings of Four Robusta Coffee (Coffea canephora Pierre. Ex. A. Froehner) Clones to Drought Stress Novie Pranata Erdiansyah; Ade Wachjar; Eko Sulistyono; Supijatno Supijatno
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 35 No 1 (2019)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v35i1.344

Abstract

Drought is one of the limiting factors for the growth and yield of coffee plants. Drought due to long dry season has caused many losses for coffee plantations. This study aimed to evaluate the response of four Robusta coffee clones to drought stress at seedling stage and determine the best clone with high tolerance to drought. The study was conducted in Indonesian Coffee and Cocoa Reseach Institute (ICCRI) Jember, East Java in November 2018–Mei 2019. The experimental design used a Randomized Block Design (RCBD) with two factors. The first factor has consisted of five levels drought through providing volume watering based coefficient and evaporation value of free water surface (Eo), namely: 0.5 Eo; 1.0 Eo; 1.5 Eo; 2.0 Eo; 2.5 Eo (control). The second factor has consisted of four Robusta coffee clones, namely: BP 409 (drought tolerant clone); BP 308; BP 939 and BP 358 (vulnerable to drought stres). The result showed that reduction in the volume of watering from 2.5 Eo until 0.5 Eo causes drought, reduce coffee growth linearly. Clone BP 409 and BP 939 had better tolerance of drought stress compared with BP 308 and BP 358. The anatomical adaptation of leaves of BP 409 to drought stress was by thickening of wax layer and palisade tissue. BP 939 thickened its leaves due to drought stress as a mechanism of adaptation to such condition.
Determination of Criteria and Drought Tolerance of Prope-Legitimate Cocoa Seed Through Polyethylene Glycol Induction Maera Zasari; Agung Wahyu Susuilo; Ade Wachjar; Sudarsono Sudarsono
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 36 No 2 (2020)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v36i2.435

Abstract

The availability of drought tolerant rootstock becomes one of solution to obtain tolerant cocoa clone at drought condition. The production of rootstock is easier and faster by using prope-legitimate seeds as plant materials. Seed germination selection phase can accelerate the obtainment of tolerant cocoa rootstocks. The tolerance trait of prope-legitimate seeds can be determined by seed germination on media induced by polyethylene glycol (PEG) solution. To determine the tolerant seeds, it is effective by using a specific character as selection criteria. This study aimed to obtain criteria and selection character to determine droughttolerant characteristic of prope-legitimate seed through PEG 6000 induction in the germination phase. The research was conducted at the Agronomy Laboratoryof the Indonesian Coffee and Cocoa Research Institute, Jember, East Java, Indonesia, used split plot design with five replications as experimental design. The main plot was solution of 0% and 6% PEG 6000, while the subplot were prope-legitimate seeds of 13 cocoa clones. The result showed that germination ability and sprout fresh weight were suitable as selection criteria to determine tolerant characteristic of prope-legitimate seeds to drought stress. Based on the value of tress susceptible index and clustering analysis, the tested prope-legitimate seeds were divided into three groups. The group of tolerant genotype consisted of Sca 06, while the group of medium tolerant were ICCRI 03, Sulawesi 02, KW 641, TSH 858, KEE 02, Sulawesi 03, and KW 617. The susceptible group consisted of six genotypes, namely ICS 60, KKM 22, KW 516, Sulawesi 01, and MCC 02.
Physio-Biochemical Characteristics of Prope Legitimate Seedlings of 13 Cocoa Clones Under Drought Stress Zasari, Maera; Wachjar, Ade; Susilo, Agung Wahyu; Sudarsono, Sudarsono
PLANTA TROPIKA Vol 11, No 2 (2023)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v11i2.14315

Abstract

The tolerant seedlings are determined by their physiological and biochemical responses. This study aimed to determine stomatal density, relative water content, and proline in prope legitimate seedlings under drought stress. The research was carried out at the Indonesian Coffee and Cocoa Research Center in July 2018 – February 2019, arranged in a randomized split-plot experimental design with six replications. The main plot was soil moisture content, and the sub-plot was the cocoa prope legitimate seedlings. The differences of mean values were tested using analysis of variance, followed by the DMRT and T-student test at 5 % level, analysis of the relative decrease in stomatal density and relative water content, proline content ratio, and the dendrogram analysis. The results showed that the cocoa seedlings under drought experienced significant changes in relative water and proline content but not stomatal density. Drought decreased in the relative water content of 50% and increased proline content in the seedlings. The relative water and proline content divided the prope legitimate seedlings into two groups. The prope legitimate seedlings from KW 516, KW 641, Scavina 06, KKM 22, KW 617, and ICCRI 03 clones were drought-tolerant.
Water Footprint Analysis of Different Techniques of Cocoa Propagation Santosa, Edi; Supijatno, Supijatno; Wachjar, Ade; Rohman, Fadil; Abdoellah, Soetanto
Journal of Tropical Crop Science Vol. 10 No. 03 (2023): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.10.03.153-165

Abstract

The nursery phase plays a crucial role in rejuvenating cocoa plantations as it significantly impacts the quality and productivity of the mature trees in the field. However, despite its significance, there remains a lack of understanding regarding its contribution to the water footprint (WF) in cocoa production. This study aims to assess the WF of various propagation techniques to promote sustainable nursery practices. Data on nurseries were collected at the Indonesian Coffee and Cocoa Research Institute in Jember, East Java, Indonesia, from June 2017 to January 2018. The results revealed that propagation accounted for a total WF ranging from 74.28 to 319.41 m3.ha-1 of established cocoa trees, with an average of 186.68 m3. This total WF consisted of 9.02 to 12.89 m3 (7.69%) attributed to seed production and 61.39 to 283.34 m3 (92.30%) attributed to the nursery phase. Among the different nursery techniques studied, the production of true seedlings exhibited the lowest WF, followed by side grafting. To optimize cocoa rejuvenation and minimize WF, it is crucial to carefully select the appropriate nursery technique. Further evaluation is necessary to explore the potential benefits of implementing precision irrigation techniques to reduce WF during the nursery phase. By focusing on sustainable nursery practices, we can enhance the overall sustainability of cocoa production.
Pengaruh Beberapa Jenis Pupuk Hayati terhadap Pertumbuhan Dua Klon Tanaman Teh (Camellia sinensis (L) O. Kuntze) Belum Menghasilkan Wachjar, Ade; Supijatno, ,; Rubiana, Dina
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 34 No. 3 (2006): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (42.846 KB) | DOI: 10.24831/jai.v34i3.1296

Abstract

The experiment was carried out to study the effect of biofertilizer on the growth of  two clones of young tea. This experiment was conducted at Cikabayan Experiment Station, Faculty of Agricuture, IPB, Bogor, from July to November 2000. This experiment was arranged in Split Plot Design with three replications.  The main factor was clone types consisted of two clones i.e.: RB 3 and Gambung 5, whereas the sub factor was biofertilizer consisted of five kinds i.e. : EMAS + 50 % inorganic fertilizer recommended dosage (i.f.r.d.), EM4 + 50 % i.f.r.d., OST + 50 % i.f.r.d., Soils Plus + 50 % i.f.r.d. and 100 % i.f.r.d. The results showed that the EMAS + 50 % i.f.r.d. and EM4 + 50 % i.f.r.d. treatments significantly increased plant height, stem diameter, leaf number, and bud length.  The application of 6.25 g EMAS per plant (equivalent with 83.125 kg/ha) + 50 % i.f.r.d.  and  10 ml  EM4 (equivalent with 6.65 l/ha) + 50 %  i.f.r.d.  could reduce  application of  inorganic  fertilizer dosage until 50 % and resulted in  the growth of the plant which  was better than that of inorganic fertilizer. In  general,  growth of the RB 3 clone was better than Gambung 5 clone.   Key words :  Biofertilizer, clones, vegetative  growth, tea
Pengaruh Kombinasi Pupuk Organik Cair dan Pupuk Anorganik serta Frekuensi Aplikasinya terhadap Pertumbuhan Tanaman Kakao (Theobroma cacao L.) Belum Menghasilkan Wachjar, Ade; Kadarisman, Luga
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 35 No. 3 (2007): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (38.918 KB) | DOI: 10.24831/jai.v35i3.1334

Abstract

This research was conducted from November 2002 to Mei 2003 in Cikabayan Research Station, Faculty of Agriculture, IPB.  The objective of this research was to determine the effect of combination of liquid organic fertilizer Bioton and inorganic fertilizer and frequency of application on the growth of two year old cocoa (Theobroma cacao L.). This research was arranged in randomized block design with two factors.  First factor was concentration of liquid organic fertilizer Bioton with five levels, i.e. 0 ml/l + 100% inorganic fertilizer recommended dosage (i.f.r.d.), 5 ml/l + 80% i.f.r.d., 10 ml/l + 60% i.f.r.d., 15 ml/l + 40% i.f.r.d., 20 ml/l + 20% i.f.r.d.; the second factors was frequency of application of liquid organic fertilizer Bioton with two levels, i.e. once every two weeks and once every four weeks.  Every treatment was replicated three times with two plants in each experimental unit.   The combination of liquid organic fertilizer Bioton and inorganic fertilizer gave the best result to the diameter of stem and the primary length of branch, and the frequency of application of liquid organic fertilizer Bioton gave no effect in all parameters.  Interaction between frequency of application of  liquid organic fertilizer Bioton and combination of fertilizer gave the effect only to the width of the biggest leaf at the sixteen weeks after the first treatment.  The spraying of liquid organic fertilizer Bioton with concentration 15 ml/l + 40% i.f.r.d., gave the best result on the growth of plant compared to other concentrations (5 ml/l + 80% i.f.r.d., 10 ml/l + 60% i.f.r.d., and 20 ml/l + 20% i.f.r.d. Frrequency of Bioton application once every four weeks was suggested.   Key words :  Cocoa, liquid organic fertilizer, inorganic  fertilizer,  concentration, rotation of application
Pengaruh Inokulasi Dua Spesies Cendawan Mikoriza Arbuskula dan Pemupukan Fosfor terhadap Pertumbuhan dan Serapan Fosfor Tajuk Bibit Kelapa Sawit ( Elaeis guineensis Jacq.) Wachjar, Ade; Setiadi, Yadi; Yunike, Ninin
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 30 No. 3 (2002): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (772.992 KB) | DOI: 10.24831/jai.v30i3.1416

Abstract

The objective of this experiment was to evaluate the effect of two Arbuscular Mychorrhizal Fungi (AMF) species and phosphorus fertilization on growth and shoot P-uptake of oil palm seedling. Experiment was conducted from September 1999 to March 2000 at Darmaga, the Cikabayan Experiment Station of Bogor Agricultural University. The experiment was arranged in Randomized Bloqk Design with five replications. The first factor was species of AMF. consisted of without AMF. inoculation with Glomus aJlJlreJlatum (OG-I05) and inoculation with Glomus manihotis (INDO-I). The secondfactor was dosage of phosphorus consisted of 0, 0.577, 1.154 and 1.734 g P/seedling.The Arbuscular Mycorrhizal Fungi did not increase height of seedling, leaves total (except on leaves total at 28 weeks after planting), shoot biomass, total biomass. and shoot P-uptake compare to the control. Shoot P-uptake was significantly decreased on seedling which was inoculated with G. manihotis compare to inoculated G. aJlJlreJlatum and control. Phosphorus fertilizer and it's interaction with species of AMF did not give any influence on growth and shoot P-uptake of the oil palm seedling. Keywords.. Mycorrhizal fungi. Phosphorus fertilization, Oil palm
Pengaruh Pupuk Organik dan Intensitas Naungan terhadap Pertumbuhan Bibit Kopi Robusta (Coffee canephora Pierre ex Froehner) Wachjar, Ade; Setiadi, Yadi; Mardhikanto, Lies Wahyuni
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 30 No. 1 (2002): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (818.324 KB) | DOI: 10.24831/jai.v30i1.1422

Abstract

The experiment was aimed at study the effect of organic fertilizer and shading inetnsity on yhe growth of Robusta coffee seedling. Seven month old seedling of hybrid variety of BP 42 and BP 358 crossing were used in the experiment was conducted at Cikabayan Experimental Station Bogor Agriculture University, from July 2000 to January 2001. The experiment was arranged in split plot dessign with three replications. the main plot was shading intensity cinsisted of 25% (N1), 50% (N2), 75% (N3), and 100% (N4) shade. The sub plot was organic fertilizer consisting of 4 g EMAS + 1/2 dosage of inorganic fertilizier (d.i.f), 4 ml EM4 + 1/2 d.i.f (P2), 4 g OST + 1/2 d.i.f(P3), 20 ml Soils Plus + 1/2 d.i.f(P4) and 1 dosage of inorganic fertilizer (P5). Organic fertilizer affected growth, as shown by height and stem diameter of seedling at early period of experiment and shoot biomass at the end of experiment compared to one effect on all variable during the experiment. Keyword : Coffee, Shading, Oerganic fertilizer
Co-Authors , Supiyatno . Sobir . Supriadi . Supriadi, . Abdoellah, Soetanto Achirul Rahman Agung Wahyu Susilo Agung Wahyu Susilo Agung Wahyu Susuilo AHMAD JUNAEDI Aiyen Tjoa Andi Besse Poleuleng Ani Kurniawati Anita Darwis Anita Th. Sinaga Anugrah, Pangeran Teguh Atikah, Nurhati Delima Ragil Kartika Dianto, Fajar Direja, Wahyu Angga Donathus Sutidjo Dr.-Ing. Dina Rubiana Widarda Edi Santosa Efendi, Darda Eko Sulistyono Gani Tarigan, Abdul Gani Grace Prilensia Damayanty Hariyadi Hariyadi Herdhata Agusta Ilyas Marzuki Indah Anita-Sari Irdika Mansur Kartika, Delima Ragil Krisantini Kusnendi, Faizal Shofwan Laksono, Purwanti Budi Launuru, Syahdin Lies Wahyuni Mardhikanto Luga Kadarisman Lukita Devy Maera Zasari Mahulette, Asri Subkhan Miska, Moch. Ega Elman Nindita, Anggi Ninin Yunike Novie Pranata Erdiansyah Odit Ferry Kurniadinata Riswan Basyri Nasution Rizki Fauziah Ramadhaini Rizkiana Anggayuhlin Rizkiana Anggayuhlin Roedhy Poerwanto Rohmah, Naelatur Rohman, Fadil Saiful Akhyar Lubis Sari, Allice Fajri Chynthia Sianturi, Vinsensia Febrina Siregar, Midian Romeo Sofyan Zaman Stefano, Indra Mario Sudarsono Sudarsono Sudarsono Sudirman Yahya Sudradjat , Sugeng Heri Suseno Suhartanto, Muhammad Rahmad Sulistianingrum, Ratih Supijatno SYAIFUL BAHRI Tengku Imam Saputra Tirsa Rachma Hastuti Ugroseno, Rene Vinsensia Febrina Sianturi Wahyu Angga Direja Yadi Setiadi