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Respon Eksplan Buku Durian Lahung (Durio dulcis) terhadap Konsentrasi BAP (Benzil Amino Purin) pada Media WPM (Woody Plant Medium): Response of Durian Lahung (Durio dulcis) Nodus Explant to BAP (Benzyl Amino Purine) Concentration on WPM (Woody Plant Medium) Nofia Hardarani; Siti Nor Zahra; Raihani Wahdah; Juharni Juharni
Daun: Jurnal Ilmiah Pertanian dan Kehutanan Vol. 10 No. 2 (2023): Daun: Jurnal Ilmiah Pertanian dan Kehutanan
Publisher : ​Institute for Researches and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/daun.v10i2.6091

Abstract

Lahung is a genus of endemic Durio in Kalimantan which currently has vulnerable status and is rare. Therefore, ex-situ conservation of this plant has begun using tissue culture techniques. Several things that need to be considered in in vitro propagation are the right type of explant, planting mediun and PGR. Stem nodes are generally chosen as explant because their availability does not depend on the season and has a fast response in bud induction. WPM medium is e medium intended for woody plants such as durian lahung. BAP is a PGR from the cytokinin group which is quite avtive and the most widely used. This research aimed to determine the best response of the durian lahung stem node culture to the BAP concentration on WPM medium. This research was designed using a rancomized block design (RBD) with one factor in the form of BAP concentration consisting of six levels, i.e. 0.0, 0.5, 1.0, 1.5, 2.0 and 2.5 ppm. Each experimental unit was repeated four times and consisted of ten planting bottles for e total of 240 planting bottles. The variables observed were the percentage of live explant aged 1-8 WPP, callus growth time, percentage of explant forming callus aged 1-8 WAP, callus and texture callus, shoot growth time and number of shoots. The results showed that callus appeared in response of the durian lahung stem node to the BAP concentration treatments.
AKURASI ESTIMASI PRODUKSI PADI DENGAN METODE NDVI BERDASARKAN SENTINEL-2 DI KABUPATEN TANAH LAUT KALIMANTAN SELATAN Rahmadi, Abdul Holid; Wahdah, Raihani; Razie, Fakhrur; Susanti, Hilda
EnviroScienteae Vol 20, No 1 (2024): ENVIROSCIENTEAE VOLUME 20 NOMOR 1, FEBRUARI 2024
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/es.v20i1.18882

Abstract

Remote sensing is a technology that is very useful in every step of the planning process and has been used in developed countries and several regions in developing countries. The aim of this research is to interpret rice production in Tanah Laut Regency using the NDVI method based on Sentinel-2 and determine the accuracy of rice production estimates in Tanah Laut Regency using the NDVI method based on Sentinel-2. Research methods include preparation, measurement principles, data collection, radiometric correction, geometric correction, creating a vegetation index, determining the best vegetation index, estimating rice production, accuracy testing, image analysis, and rice production potential. The result of this research is a rice production estimation model of y = 0.341 + 4.319 NDVI. The NDVI estimation results in Tanah Laut Regency range from 1.36 t ha-1 to 3.36 t ha-1 with an average production of 2.67 t ha-1. The results of the analysis show that there is no real difference at the 95% confidence level between the results of the NDVI estimation of rice production using Sentinel-2 imagery and the results of the field survey of rice production based on a coefficient of determination (R2) of 0.749 and a standard error (SE) of 0.29 t ha-1.
Induction of in Vitro Germination of Tandui (Mangifera rufocostata Kostrem.): Effect of Antioxidants and 2,4-dichlorophenoxyacetic Acid Fitriani, Adistina; Arifin, Yudi Firmanul; Hatta, Gusti Muhammad; Wahdah, Raihani
Biota Vol 11 No 1 (2025): Jurna Biota 2025
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/biota.v11i1.22190

Abstract

The bark of tandui (Mangifera rufocostata Kostrem.) is commonly used as a medicine for diabetes. Regeneration of this plant is difficult, and continuous harvesting of the bark leads to a decrease in the plant population. The purpose of this research is to apply tissue culture techniques for the propagation of tandui. Different antioxidants (KNO3, polyvinylpyrrolidone (PVP), Murashige and Skoog (MS) media + PVP, and MS + ascorbic acid) and varying concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) (0.8, 1.0, 1.2, and 1.4 mg L–1) were tested for embryogenic tandui. The application of KNO3 and PVP suppressed the formation of browning in the media and explant of tandui. Soaking the explants in KNO3 and PVP reduce browning to 35% and 20%, respectively. The results also showed that increasing the concentration of 2,4-D enhanced the percentage of embryogenic tandui. Supplementing the media with 1.4 mg L–1 2,4-D resulted in 90% of embryogenic tandui. This study demonstrates that pre-soaking explants in antioxidants significantly reduces media browning, and supplementation of MS media with 2,4-D enhance embryogenic process. Thus, micropropagation of tandui could be achieved on a commercial basis.
Pelatihan Budidaya Tanaman secara Hidroponik kepada Tenaga Penyuluh dan Kelompok Tani Kecamatan Bumi Makmur, Kabupaten Tanah Laut: Hydroponic Plant Cultivation Training for Extension Workers and Farmer Groups in Bumi Makmur District, Tanah Laut Regency Jawak, Gani; Ismuhajaroh, Bakti Nur; Rusmayadi, Gusti; Juharni, Juharni; Wahdah, Raihani; Langai, Bambang Fredrickus; Fadhiel, Muhammad Ihsan; Rambe, Mikhael Wesley; Nababan, Endang Mayjela Enjelika; Yakub, Yakub
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 10 No. 1 (2025): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v10i1.8253

Abstract

Bumi Makmur District is located in a swampy area with the main livelihood being rice cultivation. Vegetables cannot be produced in this region because the swamp soil is acidic and pyrite. One of the solutions offered to meet the needs of vegetables is hydroponic cultivation. Hydroponic training was carried out to increase public knowledge about the cultivation of vegetable crops with hydroponics by socialization and practice of wick system hydroponics. The training participants were Field Agricultural Extension Workers and Farmer Groups, carried out in the hall of the Bumi Makmur Agricultural Extension Center. The number of training participants was 24 people, consisting of 20 men and 4 women. All of the training participants were >18 years old with education levels ranging from junior high school to Master's level. The trainees who had heard the term hydroponics through the internet, radio/television, print media, family, and school were 91.67%. Participants who had cultivated vegetable crops with hydroponics were only 4.17% and planned to cultivate plants hydroponically was 83.33%. A total of 93.83% of participants had never and 95.83% were interested in participating in hydroponic training. The final survey showed that 95.83% of participants wanted to take part in advanced training on hydroponic systems and techniques with hands-on practice methods and a training duration of 2-3 days. Only 58.33% of participants rated hydroponic training as very important. The results of the basic ability test about the participants' knowledge of hydroponics showed an increase in the average score from the initial 50.00 to 68.7.
Pengaruh Varietas dan Jarak Tanam terhadap Efisiensi Radiasi, Pertumbuhan dan Hasil Jagung Manis (Zea mays saccharata Sturt.) Di Lahan Rawa Lebak Majedi; Gusti Rusmayadi; Wahdah, Raihani
Rawa Sains: Jurnal Sains STIPER Amuntai Vol 12 No 1 (2022): Vol 12 No 1 (2022): Edisi Juni 2022
Publisher : LPPM Sekolah Tinggi Ilmu Pertanian Amuntai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36589/rs.v12i1.246

Abstract

In Indonesia, corn is a plant that has a function as a source of food, a source of feed and the main ingredient for industry. However, it has not been able to meet market demand for corn because it is constrained by several factors such as lowland swamp land which is used as a place of cultivation with all the problems, namely periodically inundated land, low soil fertility, high soil acidity, toxic substances, lack of micro nutrients, organic matter still raw and others. The objectives of the study were: 1) Analyzing the interaction of varieties with spacing on radiation efficiency, growth and yield of sweet corn (Zea mays saccharata Sturt.) in lowland swamp land. 2) Analyzing the best varieties and the best spacing for radiation efficiency, growth and yield of sweet corn (Zea mays saccharata Sturt.) in lowland swamp land. This research was conducted in Muara Tapus Village, North Hulu Sungai Regency, South Kalimantan Province in May - August 2021 using the Factorial Randomized Design method. The first factor is the sweet corn variety (V) consisting of four levels, namely exotic variety (v1), jamboree variety (v2), talent variety (v3) and ganebo variety (v4). The second factor is sweet corn planting distance (J) consisting of three levels, namely 75 cm x 20 cm (j1), 75 cm x 25 cm (j2) and 75 cm x 30 cm (j3). The results showed that the interaction between varieties and spacing did not affect all observation variables. Varieties affect the observed variables of plant height, number of leaves, leaf area index, plant growth rate and number of rows per ear. Spacing affects the observed variables of plant height, leaf area index and plant growth rate. The best variety is the talent variety (v3) and the best spacing is 75 cm x 20 cm (j1) and the radiation utilization efficiency value is 1.5584 g.MJ-1.
Pengaruh Konsentrasi Ekstrak Akar Eceng Gondok terhadap Pembungaan, Komponen Hasil, dan Hasil Dua Varietas Terung (Solanum melongean L.) oktaviani, reni; Wahdah, Raihani; Purnomo, Joko
AgroGreen Jurnal AgroGreen, Vol. 2, Nomor 1, Januari 2026
Publisher : Jurusan Agronomi, Fakultas Pertanian, Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pembentukan buah partenokarpi dapat dilakukan dengan induksi menggunakan zat pengatur tumbuh, seperti giberelin. Salah satu sumber hormon giberelin alami terdapat pada tanaman eceng gondok sehingga dapat dimanfaatkan sebagai pengganti hormon giberelin sintetis. Penelitian ini dilaksanakan sejak Januari – April 2024 di lahan percobaan SMK-PP Negeri Banjarbaru. Penelitian ini menggunakan rancangan lingkungan Rancangan Acak Kelompok faktorial dua faktor dengan rancangan perlakuan Rancangan Petak Terbagi. Varietas sebagai petak utama dan konsentrasi ekstrak akar eceng gondok sebagai anak petak. Peubah yang diamati, yaitu umur berbunga, jumlah bunga, jumlah rangkaian bunga, bobot segar per buah, bobot daging buah per buah, panjang buah, diameter buah, jumlah biji, dan bobot kering biji. Terdapat interaksi antara konsentrasi ekstrak akar eceng gondok dengan varietas berpengaruh sangat nyata terhadap peubah jumlah biji, kombinasi terbaik terhadap jumlah biji adalah varietas Yumi F1 dan konsentrasi ekstrak 250 ppm yang menghasilkan jumlah biji terendah sebanyak 270,64 butir. Sementara itu, faktor tunggal menunjukkan tidak berpengaruh pada semua peubah.
Effect of Storage Medium Composition on the Viability of Antagonistic Bacteria Against Stem Rot Disease Hidayati, Nurul; Salamiah, Salamiah; Wahdah, Raihani; Razie, Fahrur; Arfianto, Fahruddin
Prisma Sains : Jurnal Pengkajian Ilmu dan Pembelajaran Matematika dan IPA IKIP Mataram Vol. 14 No. 1: January 2026
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/j-ps.v14i1.18727

Abstract

Stem rot is a major constraint in sweet corn and maize production, especially under humid conditions that favor pathogen development. Although antagonistic bacteria offer a sustainable alternative to chemical control, their field use is often limited by viability loss during room-temperature storage, making carrier formulation critical for shelf-life and product reliability. This study evaluated the effect of biochar–peat carrier composition on the storage viability of five antagonistic bacterial isolates for stem rot management in sweet corn. A two-factor factorial experiment (4 carrier compositions × 5 isolates) with three replications (60 experimental units) was conducted under laboratory conditions. Carriers were prepared as biochar: peat mixtures (v/v) of 3:1, 1:1, 1:3, and 100% peat. Each carrier unit (10 g) was inoculated with 1 mL bacterial suspension (≈10⁸ cfu mL⁻¹), sealed, and stored at room temperature (28–29 °C). Viability was quantified at 30, 60, and 90 days after inoculation (DAI) using serial dilution and plate counts on TSA, expressed as cfu g⁻¹. Data at 60 DAI were analyzed by factorial ANOVA including block (Group), medium (M), isolate (P), and M×P effects, followed by LSD (5%) for mean separation using SPSS 25. At 30 DAI, bacterial densities were high and did not differ among carriers, indicating comparable short-term support across media. At 60 DAI, carrier composition significantly affected bacterial density, whereas isolate and M×P interaction effects were not significant, indicating a general carrier-driven response across isolates. The biochar-rich carrier (3:1) maintained the highest mean population (8.844 × 10⁷ cfu g⁻¹). By 90 DAI, all treatments declined, yet the 3:1 carrier retained the highest density (1.089 × 10⁷ cfu g⁻¹). Overall, biochar-enriched carriers, particularly the 3:1 biochar: peat mixture, better preserved antagonistic bacterial viability under non-refrigerated storage up to 90 days.
DAMPAK INSEKTISIDA NABATI DAUN MIMBA DAN INSEKTISIDA SINTETIK TERHADAP KERUSAKAN DAUN, PERTUMBUHAN, DAN HASIL TANAMAN SAWI (Brassica juncea L.) Maudodi, Raz Sayyid; Priatmadi, Bambang Joko; Wahdah, Raihani; Saidy, Akhmad Rizalli
EnviroScienteae Vol 22, No 1 (2026): ENVIROSCIENTEAE VOLUME 22 NOMOR 1, FEBRUARI 2026
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/es.v22i1.25306

Abstract

The decline in mustard (Brassica juncea L.) productivity in recent years has been associated with increasing pest pressure and uncontrolled insecticide use. This study aimed to evaluate the effects of neem leaf–based botanical insecticide and the synthetic insecticide methomyl applied at three spraying frequencies on insect damage intensity, number of leaves, plant height, and fresh weight of mustard. The experiment was arranged in a randomized block design with nested treatments and a separate control. The results showed that the application of both botanical and synthetic insecticides reduced leaf damage intensity and enhanced vegetative growth and fresh weight compared to the control. At 21 days after planting, the neem-based insecticide demonstrated a better ability to suppress pest attack intensity (9.39%) than the synthetic insecticide methomyl (11.29%). The application of methomyl at a spraying interval of three days produced the highest fresh weight (155.08 g) compared to other spraying frequencies.