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ISOLASI DAN IDENTIFIKASI BAKTERI AEROB YANG BERPOTENSI MENYEBABKAN INFEKSI NOSOKOMIAL DI IRINA E RSUP PROF. DR. R. D. KANDOU MANADO Lumentut, Beatrix A.; Waworuntu, Olivia A.; Homenta, Heriyannis
eBiomedik Vol 4, No 1 (2016): eBiomedik
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/ebm.4.1.2016.11051

Abstract

Abstract: Nosocomial infections or now called Healthcare Associated Infection (HAIs) is an infection that occurs in patients during treatment in a hospital or other health care facilities that do not show symptoms of infection while in the hospital. Nosocomial infection is an important issue in health care that continues to increase especially in children who are under treatment in hospital. This study aims to determine the source and identify aerobic bacteria that could potentially cause nosocomial infections in walls, floors, medical equipment, and air in Irina E Prof. Dr. R. D. Kandou Manado General Hospital. Design methods for this research using cross sectional study with a descriptive prospective approach. Samples were bacteria in walls, floors, medical equipment, and air of Irian E Prof. Dr. R. D. Kandou Manado General Hospital. The results showed that from the 24 samples taken, there were 6 bacteria found which is Enterobacter cloacae (41,7%), Bacillus subtilis (20,8%), Serratia liquefaciens (16,7%), Enterobacter agglomerans (12,5%), Lactobacillus (4,17%), dan Staphylococcus sp. (4,17%),Keywords: Irina E, nosocomial infections, aerobic bacteria.Abstrak: Infeksi nosokomial atau yang sekarang disebut Healthcare Associated Infection (HAIs) adalah infeksi yang terjadi pada pasien selama proses perawatan di rumah sakit atau fasilitas kesehatan lainnya yang tidak menunjukkan gejala infeksi saat masuk rumah sakit. Infeksi nosokomial merupakan masalah penting dalam pelayanan kesehatan yang terus meningkat terlebih pada anak-anak yang sedang dalam perawatan di rumah sakit. Penelitian ini bertujuan untuk mengetahui sumber dan mengidentifikasi bakteri aerob yang berpotensi menyebabkan infeksi nosokomial di dinding, lantai, peralatan medis, dan udara di Irina E RSUP Prof. Dr. R. D. Kandou Manado. Desain penelitian ini menggunakan studi cross sectional dengan pendekatan deskriptif prospektif. Sampel penelitian ialah bakteri pada dinding, lantai, peralatan medis, dan udara di Irina E RSUP Prof. Dr. R. D. Kandou Manado. Hasil penelitian memperlihatkan dari 24 sampel yang diambil, terdapat 6 bakteri yaitu Enterobacter cloacae (41,7%), Bacillus subtilis (20,8%), Serratia liquefaciens (16,7%), Enterobacter agglomerans (12,5%), Lactobacillus (4,17%), dan Staphylococcus sp. (4,17%),Kata kunci: Irina E, infeksi nosokomial, bakteri aerob
Pola bakteri aerob yang berpotensi menyebabkan infeksi nosokomial di ruangan Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO) RSUP Prof. Dr. R. D. Kandou Manado Matoka, Rizka; Waworuntu, Olivia; Rares, Fredine
e-Biomedik Vol 4, No 2 (2016): eBiomedik
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/ebm.v4i2.14643

Abstract

Abstract: Nosocomial infection or Health-care Associated Infection (HAIs) according to the WHO in 2011 are infection to patient acquired during a hospital, after ≥48 hours and ≤30 days after out of the hospital. Factor of infection can be differentiated between patients, nurses, doctors, hospital employed, hospital environment and patient visitor or family. The purpose to know the pattern of aerobic bacteria that could potentially to involve nosocomial infection in the Emergency room of Obstetric and Gynecology (IRDO) Prof. Dr. R. D. Kandou Manado. Type a descriptive observational research with cross sectional study approach. The sampling on the walls, floors, medical equipment, room furniture and the air in the IRDO Prof. Dr. R. D. Kandou Hospital Manado. The results of the 27 samples found bacteria Bacillus sp.(46,55%), Staphylococcus sp. (18,97%), Streptococcus sp. (10,34%), Klebsiella pneumoniae (8,62%), Enterobacter aerogenes, Serratia liquefaciens, Enterobacter cloacea, proteus sp. (3,45%), Neisseria sp. (1,72%).Conclusion: The most aerobic bacteria in the Emergency room of Obstetric and Gynecology (IRDO) Prof. Dr. R. D. Kandou Manado is Bacillus sp.(46,55%).Keywords: Emergency Obstetric and Gynecology, nosocomial infection, aerobic bacteria. Abstrak: Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO) adalah suatu instalasi penanggulangan awal penderita rawat darurat untuk mencegah kematian, dalam menangani kesehatan reproduksi wanita saat tidak hamil ataupun pada saat hamil, persalinan, dan nifas. Infeksi nosokomial atau Health-care Associated Infection (HAIs) menurut WHO 2011 merupakan infeksi yang didapat pasien selama di rumah sakit, setelah ≥ 48 jam dan ≤ 30 hari setelah keluar dari rumah sakit. Faktor terjadinya infeksi bisa dibebakan antar pasien, perawat, dokter, karyawan rumah sakit, lingkungan rumah sakit, dan pengunjung atau keluarga pasien. Tujuan untuk mengetahui pola bakteri aerob yang berpotensi menyebabkan infeksi nosokomial pada ruangan Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO) RSUP Prof. Dr. R. D. Kandou Manado. Jenis penelitian deskriptif observational dengan pendekatan studi cross sectional. Pengambilan sampel pada dinding, lantai, peralatan medis, perabotan ruangan, udara di ruangan Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO) RSUP Prof. Dr. R. D. Kandou Manado. Hasil penelitian dari 27 sampel ditemukan bakteri Bacillus sp.(46,55%), Staphylococcus sp. (18,97%), Streptococcus sp. (10,34%), Klebsiella pneumoniae (8,62%), Enterobacter aerogenes, Serratia liquefaciens, Enterobacter cloacea, proteus sp. (3,45%), Neisseria sp. (1,72%). Simpulan: Bakteri aerob di ruangan Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO) RSUP Prof. Dr. R. D. Kandou Manado yang terbanyak ialah Bacillus sp.(46,55%).Kata kunci: Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO), Infeksi nosokomial, bakteri aerob
POLA BAKTERI AEROB PADA PASIEN DENGAN DIAGNOSIS OTITIS MEDIA SUPURATIF AKUT DI POLIKLINIK THT-KL RSUP. PROF. DR. R. D. KANDOU MANADO Mamonto, Nur Dinih; Porotu’o, John; Waworuntu, Olivia
e-Biomedik Vol 3, No 1 (2015): eBiomedik
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/ebm.v3i1.6844

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Abstract: Otitis media is an inflammation of part or all of the middle ear mucosa. Eustachi tube obstruction is a basic causative factor in acute otitis media. The tract infections can cause swelling around the channel, crunch, and as a result formed pus in the middle ear. The purpose of research is to determine the pattern of aerobic bekteri in patients diagnosis of Acute suppurative otitis media in Polyclinic Hospital THT-KL. PROF. dr. R. D. Kandou Manado. This study is a descriptive study population of all patients in the diagnosis of acute suppurative otitis media by making a purposive sampling technique obtained 20 samples of patients with acute suppurative otitis media. Then, 20 samples were collected for microbiological examination by culture techniques and biochemical tests. The results of this study were 20 patient samples obtained Acute suppurative otitis media age 0-5 years (8 samples; 42%), 6-10 years (6 samples; 27%), 11-20 years (1 sample; 5%), ≥ 20 years (5 samples; 26%); male gender - male (9 samples; 55%), women (9 samples; 45%). Twenty samples examined, all showed growth of bacteria and obtained seven bacterial species and one species of candida. 3 obtained from the gram-positive bacteria, namely Streptococcus sp culture (7 samples; 35%), Staphylococcus sp (4 samples; 20%), Bacilus subtilis (2 samples; 10%) 4 gram-negative bacteria Enterobacter Aglomerans (2 samples; 10% ), Enterobacter cloacae (1 sample; 5%), Seretia Rubidaea (1 sample; 5%), Proteus vulgaris (2 samples; 10%), Candida (1 sample; 5%). Conclusion The research found most bacteria causing acute suppurative otitis media, namely Streptococcus, the largest age group experienced acute suppurative otitis media, namely children’s (0-5 years).Keywords: acute suppurative otitis media, aerobic bacteriaAbstrak: Otitis media adalah peradangan sebagian atau seluruh mukosa telinga tengah. Obstruksi tuba eustachi merupakan suatu faktor penyebab dasar pada otitis media akut. Infeksi di saluran tersebut dapat menyebabkan pembengkakan di sekitar saluran, tersumbatnya saluran, dan sebagai hasilnya terbentuklah nanah dalam telinga tengah. Tujuan penelitian adalah untuk mengetahui pola bekteri aerob pada pasien diagnosis Otitis Media Supuratif Akut di Poliklinik THT–KL RSUP. PROF. dr. R. D. Kandou Manado. Penelitian ini merupakan penelitian deskriptif dengan populasi seluruh pasien yang di diagnosis Otitis Media Supuratif Akut dengan teknik pengambilan purposive sampling didapatkan 20 sampel penderita Otitis Media Supuratif Akut. Kemudian, 20 sampel dikumpulkan untuk dilakukan pemeriksaan mikrobiologi dengan teknik kultur dan uji biokimia. Hasil dari penelitian ini adalah 20 sampel pasien Otitis Media Supuratif Akut didapatkan umur 0 – 5 tahun (8 sampel; 42%), 6 - 10 tahun (5 sampel; 27%), 11 – 20 tahun (1 sampel; 5%), ≥ 20 tahun (5 sampel; 26%); jenis kelamin laki – laki (9 sampel; 55%), perempuan (9 sampel; 45%). Duapuluh sampel yang diperiksa, seluruhnya menunjukkan pertumbuhan bakteri dan didapatkan 7 spesies bakteri dan 1 spesies candida. Didapatkan 3 bakteri gram positif dari hasil kultur yaitu Streptococcus sp (7 sampel; 35%), Staphylococcus sp (4 sampel; 20%), Bacilus subtilis (2 sampel; 10%) 4 bakteri gram negatif Enterobacter Aglomerans (2 sampel; 10%), Enterobacter cloacae (1 sampel; 5%), Seretia Rubidaea (1 sampel; 5%), Proteus Vulgaris (2 sampel; 10%), Candida (1 sampel; 5%). Kesimpulan penelitian didapatkan bakteri terbanyak penyebab Otitis Media Supuratif Akut yaitu Streptococcus,dan kelompok umur terbanyak yaitu anak – anak (0 – 5 Tahun).Kata kunci: otitis media supuratif akut, bakteri aerob
Sensitivitas dan Spesifisitas Rapid Diagnostic Test Malaria sebagai Diagostik Laboratorium Malaria di RSUD Noongan Wowor, Mayer Ferdinand; Waworuntu, Olivia Amelia; Polii, Hedison; Bernadus, Janno Berty Bradly
JKK (Jurnal Kedokteran Klinik) Vol 3, No 2 (2019): JURNAL KEDOKTERAN KLINIK
Publisher : FAKULTAS KEDOKTERAN UNSRAT

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Abstract

ABSTRAKMalaria merupakan masalah kesehatan di daerah tropis dan subtropik seperti Brazil, seluruh sub sahara Afrika dan Asia Tenggara karena mempengaruhi angka kesakitan bayi, balita, dan ibu melahirkan serta menimbulkan Kejadian Luar Biasa (KLB). Pemeriksaan Rapid Diagnostic Test (RDT) merupakan suatu pemeriksaan laboratorium yang digunakan untuk mendiagnosa penyakit malaria berdasarkan atas deteksi antigen parasit malaria di dalam darah dengan menggunakan prinsip immunochromatographic. RDT membantu dalam deteksi malaria yang lebih handal dan cepat untuk mendeteksi infeksi malaria bahkan di daerah  terpencil dengan akses terbatas ke layanan mikroskop berkualitas baik.  Penelitian ini bertujuan untuk mengetahui efektifitas Rapid Diagnostic Test  (RDT) dalam deteksi  malaria di RSUD Noongan dann diharapkan mampu memberikan manfaat bagi instansi kesehatan dalam pengembangan penanggulangan malaria di Indonesia.  Penelitian yang dilakukan adalah survei deskriptif dengan menggunakan Cross sectional , serta Uji laboratorium untuk Uji diagnostik RDT malaria.  Efekivitas dari alat uji yang  ada  adalah  memiliki sensitifitas  90 %, spesifisitas 97%, nilai duga positifKata kunci :  malaria, RDT, sensitivitas ,spesifisitas ABSTRAK               Malaria is a health problem in tropical and subtropical regions such as Brazil, all sub-Saharan Africa and Southeast Asia because it affects the morbidity of infants, toddlers, and mothers giving birth and causes Extraordinary Events (KLB). Rapid Diagnostic Test (RDT) is a laboratory examination that is used to diagnose malaria based on the detection of malaria parasite antigens in the blood by using the immunochromatographic principle. RDT helps in more reliable and faster malaria detection to detect malaria infections even in remote areas with limited access to good quality microscopy services. This study aims to determine the effectiveness of the Rapid Diagnostic Test (RDT) in malaria detection at Noongan District Hospital and is expected to be able to provide benefits for health agencies in developing malaria prevention in Indonesia. The research conducted was a descriptive survey using cross sectional, as well as laboratory tests for malaria RDT diagnostic test. The effectiveness of the existing test equipment is 90% sensitivity, 97% specificity, positive predictive valueKeyword   :     Malaria, RDT, sensitivitas,  spesifisitas                  
IDENTIFIKASI BAKTERI PADA DEPOT AIR MINUM ISI ULANG DI KOTA MANADO Rumondor, Perisai P.; Porotu’o, John; Waworuntu, Olivia
e-Biomedik Vol 2, No 2 (2014): eBiomedik
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/ebm.v2i2.5518

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Abstract: Water is a chemical compound that has a very important role for the life of humans and other living creatures but also serve as a medium of transmission of diseases. Refillable drinking water has become a common choice for the people in Indonesia, including in the city of Manado. One of the diseases caused by drinking poor quality water is diarrhea. To determine the condition of the contaminated refillable drinking water, a research or clinical testing in the laboratory is needed. The purpose of this research was to determine the pattern of bacteria in refillable drinking water. This study uses a descriptive research method with a cross sectional approach. Samples were taken from 20 refillable drinking water depots in Manado city that have met the inclusion criteria. Identification of bacteria performed with the culture medium. The results showed 4 samples contained gram positive bacteria, 5 samples contained gram negative bacteria and 11 samples contained a mixture of gram positive and gram negative. Gram positive bacteria consists of three species which are Bacillus subtilis (14 samples; 42,42%), Streptococcus spp. (1 sample; 3,03%) and Staphylococcus spp. (1 sample; 3,03%) and. Six species of gram negative bacteria which are Shigella (1 sample; 3,03%), Diplococcus (1 sample; 3,03%), Enterobacter cloacae (2 samples; 6,06%), Enterobacter agglomerans (2 samples; 6,06%), Pseudomonas spp. (4 samples; 12,12%), and Proteus vulgaris (7 samples; 21,21%). The conclusion of this research most types bacteria of found were Bacillus subtilis. Keywords: bacteria, refillable drinking water    Abstrak: Air merupakan senyawa kimia yang sangat penting fungsinya bagi kehidupan umat manusia dan mahkluk hidup lainnya tapi juga dapat berperan sebagai media penularan penyakit. Air minum isi ulang telah menjadi pilihan umum masyarakat Indonesia, termasuk di kota Manado.Salah satu penyakit yang disebabkan oleh air minum yang kualitas mikrobiologisnya buruk adalah diare.Untuk mengetahui kondisi terkontaminasi tidaknya air minum isi ulang diperlukan penelitian atau pengujian secara klinis di laboratorium.Tujuan dari penelitian ini adalah untuk mengetahui pola bakteri pada air minum isi ulang. Penelitian ini menggunakan metode penelitian deskriptif dengan pendekatan cross sectional. Sampel diambil dari 20 depot air minum isi ulang di Kota Manado yang telah memenuhi kriteria inklusi. Identifikasi bakteri dilakukan dengan media kultur. Hasil penelitian menunjukkan 4 sampel terdapat bakteri gram positif, 5 sampel terdapat gram negatif dan 11 sampel terdapat campuran bakteri gram positif dan gram negatif.Bakteri gram positif terdiri dari 3 spesies yaitu Bacillus subtilis (14 sampel; 42,42%), Streptococcus spp. (1 sampel; 3,03%)dan Staphilococcus spp. (1 sampel; 3,03%). Enam bakteri spesies gram negatif yaitu Shigella (1 sampel; 3,03%), Diplococcus (1 sampel; 3,03%), Enterobacter cloacae (2 sampel; 6,06%), Enterobacter agglomerans (2 sampel; 6,06%), Pseudomonas spp. (4 sampel; 12,12%) dan Proteus vulgaris (7 sampel; 21,21%). Kesimpulan pada penelitian ini jenis bakteri terbanyak yang ditemukan adalahBacillus subtilis. Kata Kunci: bakteri, air minum isi ulang
POLA BAKTERI PADA URIN PASIEN YANG MENGGUNAKAN KATETER URETRA DI RUANG PERAWATAN INTENSIF RSUP PROF. DR. R. D. KANDOU MANADO Chandra, Melisa P.; Waworuntu, Olivia; Buntuan, Velma
e-Biomedik Vol 2, No 2 (2014): eBiomedik
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/ebm.v2i2.5048

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Abstract: Urinary catheterization as a part of invasive procedure application poses the risk of nosocomial infection to intensive care unit patients. The probability of nosocomial infection increases in accordance to the period of catheterization; longer catheterization time yields higher chances for microbial contamination on urinary catheter as catheterization provides an opening for microbial invasion into the urinary track. There are various pathogens commonly associated with catheterization including Escherichia coli, Klebsiella pneumonia, Proteus mirabili, Pseudomonas aeruginosa, Enterobacter, Staphylococcus aureus, Enterococcus fecalis, Serratia and Candida. The main objective of this research project is to observe bacterial pattern of urine from catheterized intensive care unit patients in the Prof. DR. R. D. Kandou Public Hospital Manado. A total of 20 urine samples were obtained, one sample from each individual catheter over 2 months period. Each collected sample is subsequently passed on to microbiology laboratory to undergo bacteria identification process via culture media. The preliminary results show that the identified bacterial pattern is Staphylococcus aureus, Candida, Streptococcus, Diplococcus, Proteus vulgaris, Lactobacillus, Bacillus subtilis, Enterobacter aglomerans, Citrobacter freundii dan Shigella. Following an in-depth multi-dimensional analysis of preliminary research results, the conclusion can be drawn that the most commonly found bacteria is Staphylococcus aureus. This study is part of growing body of microbiology research in catheter-related bacterial pattern and nosocomial infection; this research project will contribute to future research on similar topics. Keywords: bacteria, bacterial pattern, urinary catheter, nosocomial infection, intensive care unit.     Abstrak: Kateterisasi urin adalah bagian dari penggunaan prosedur invasif  membawa resiko infeksi nosokomial bagi pasien yang dirawat di ruang perawatan intensif.  Semakin lama kateter terpasang maka peluang kateter terkontaminasi oleh mikroba semakin besar, karena penggunaan kateter memungkinakan  jalur masuk mikroba ke dalam saluran kemih.Terdapat berbagai macam  patogen yang menyebabkan ISK yang diasosiasikan dengan kateterisasi termasuk Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, Enterobacter, Staphylococcus aureus, Enterococcus fecalis, Serratia, Candida. Tujuan dari penelitian ini adalah untuk mengetahui pola bakteri pada urin pasien yang menggunakan kateter uretra di ruang perawatan intensif RSUP. Prof. Dr. R. D. Kandou Manado sebanyak 20 sampel. Sampel di ambil dari urin kateter pasien yang di rawat di ruang perawatan intensif. Identifikasi bakteri dilakukan dengan menggunakan media kultur. Hasil dari kultur urin menunjukan pola bakteri yang berhasil diidentifikasi yakni Staphylococcus aureus, Candida, Streptococcus, Diplococcus, Proteus vulgaris, Lactobacillus, Bacillus subtilis, Enterobacter aglomerans, Citrobacter freundii dan Shigella. Dari hasil kesimpulan pada penelitian ini didapatkan  jenis bakteri yang paling banyak ditemukan adalah Staphylococcus aereus. Mengikuti analisis data hasil penelitian beberapa saran tentang proses penelitian lebih lanjut akan diusulkan. Kata kunci: bakteri, pola kuman, kateter uretra, infeksi nosocomial, ruang perawatan intensif.
PERBEDAAN INDEKS PLAK PADA PENGGUNA ALAT ORTODONTIK CEKAT YANG MENGGUNAKAN SIKAT GIGI KHUSUS ORTODONTIK DENGAN DAN TANPA OBAT KUMUR Windy Tjiali; PS Anindita; Olivia Waworuntu
JURNAL ILMIAH SAINS Volume 15 Nomor 2, Oktober 2015
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (350.254 KB) | DOI: 10.35799/jis.15.2.2015.9571

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PERBEDAAN INDEKS PLAK PADA PENGGUNA ALAT ORTODONTIK CEKAT YANG MENGGUNAKAN SIKAT GIGI KHUSUS ORTODONTIK DENGAN DAN TANPA OBAT KUMUR ABSTRAK Perawatan ortodontik khususnya penggunaan alat ortodontik cekat memiliki bentuk yang rumit sehingga mempermudah melekatnya plak lebih lama dan dapat meningkatkan resiko karies, gingivitis, dan penyakit periodontal. Salah satu cara untuk mengurangi resiko yang ada adalah dengan melakukan kontrol plak. Kontrol plak dapat dilakukan secara mekanis (sikat gigi khusus ortodontik) dan kimiawi (obat kumur). Penelitian ini bertujuan untuk mengetahui perbedaan indeks plak pada pengguna alat ortodontik cekat yang menggunakan sikat gigi khusus ortodontik dengan tambahan obat kumur dan tanpa obat kumur. Jenis penelitian ini adalah eksperimen lapangan dengan rancangan the eqiuevalent material group, pretest and posttest design. Penelitian ini dilakukan dengan sampel mahasiswa angkatan 2011-2012 Program Studi Pendidikan Dokter Gigi Universitas Sam Ratulangi Manado sebanyak 33 orang. Pengambilan sampel dengan menggunakan metode purposive sampling. Data  dianalisis menggunakan uji dependent t-test. Hasil analisis menunjukkan  perbedaan penurunan indeks plak yang signifikan (p<0,05). Dapat disimpulkan bahwa terdapat perbedaan indeks plak pada pengguna alat ortodontik cekat yang menggunakan sikat gigi khusus ortodontik dengan tambahan obat kumur dan tanpa obat kumur. Kata kunci : ortodontik cekat, sikat gigi khusus ortodontik, obat kumur, indeks plak.   THE DIFFERENCE OF PLAQUE INDEX ON FIXED ORTHODONTIC USERS THAT USED ORTHODONTIC TOOTHBRUSH WITH AND WITHOUT MOUTHWASH ABSTRACT Orthodontic treatment especially fixed orthodontic appliance has a complex form that can simplify the attachement of plaque longer and could increase the risk of caries, gingivitis, and periodontal disease. One way to reduce the risk is plaque control. Plaque control can be done mechanically (orthodontic toothbrush) and chemically (mouthwash). This research aimed to know the difference plaque index on users of fixed orthodontic appliance that used orthodontic toothbrush with mouthwash and without mouthwash.This research is field experiments to a draf the eqiuevalent material group, pretest and posttest design. This research is done with a sample of 33 people of 2011-2012 dentist students of Sam Ratulangi University. The sample was collected by purposive sampling method. Data was analyzed by the dependent t-test. The result showed that there was significant decrease of plaque index (p<0,05). In conclusion, there is a differenceplaque index on users of fixed orthodontic appliance that used orthodontic toothbrush with  mouthwash and without mouthwash. Key words: Fixed orthodontic, orthodontic toothbrush, mouthwash, plaque index.
Kualitas hidup dengan kualitas tidur remaja SMA Katolik Rex Mundi Manado di masa pandemi COVID-19 Rahmat Aridi; Henry M. F. Palandeng; Ronald I. Ottay; Olivia Waworuntu
JURNAL KEDOKTERAN KOMUNITAS DAN TROPIK JKKT Volume 10 Nomor 1 (2022)
Publisher : UNIVERSITAS SAM RATULANGI

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Background: Adolescent is a period of growth that is most unique since there are very large changes covering physical, psychological and social development. The growth and development of adolescents are influenced by the quality of life with physical activity. However, the COVID-19 epidemic has severely limited the physical activity of adolescents, which has an impact on their quality of life and growth.Objective: The general aim of this study was to discover the quality of adolescent life and physical activity during the pandemic and the specific objective was to find out an overview of the quality of life, physical dimensions, psychological dimensions, social dimensions, environmental dimensions in physical activity.Method: This study used descriptive research with a cross-sectional design and it also used the cluster random sampling technique and simple random sampling by distributing WHOQOL-BREF questionnaires and the Global School-based Student Health Survey (GSHS) through google form.Results: Based on the 91 samples, it indicated that the physical health dimension of students with light physical activity, most of the good quality of life was 37.4, the psychological dimension with moderate physical activity, most of the good quality of life was 29.7, the social dimension with the most moderate student activity was 31.9.Conclusion: Students of SMP Negeri 2 Manado had a good and moderate quality of life, while their physical activity was moderate in all dimensions.Keywords: quality of life, physical activity, adolescents, COVID-19. 
Identifikasi Pola Bakteri dan Uji Sensitivitas Antibiotik di Poli Infeksi dan Imunologi Rumah Sakit Mata Provinsi Sulawesi Utara Matandung, Lewis F.; Rares, Fredine E. S.; Waworuntu, Olivia A.
e-CliniC Vol. 12 No. 3 (2024): e-CliniC
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/ecl.v12i3.53539

Abstract

Abstract: Infections that occur in hospitals can be caused by bacteria, viruses, and fungi. Infections that occur in hospitals in less than 48-72 hours after the patient is admitted to the hospital are called Healthcare Associated Infections (HAIs) or commonly known as nosocomial infections. Transmission of nosocomial infections is usually through direct contact, droplets, and through health workers in the hospital, as well as through tools or media in the hospital. This study aimed to determine bacterial patterns and sensitivity tests in the infection and immunology poly of the North Sulawesi Provincial Eye Hospital. This was an observational and descriptive study with a cross-sectional design to identify bacterial patterns and sensitivity tests in the infection and immunology poly of the North Sulawesi Provincial Eye Hospital. The collection of 10 samples was carried out in the poly infection and immunology. The results obtained four types of bacteria, namely: Staphylococcus sp, Staphylococcus aureus, Bacillus sp, and Streptococcus sp. In conclusion, the four types of bacteria are sensitive to meropenem and resistant to erythromycin. Keywords: nosocomial infection; bacterial identification; sensitivity test   Abstrak: Infeksi yang terjadi di rumah sakit dapat disebabkan oleh bakteri, virus, dan jamur. Infeksi yang terjadi di rumah sakit dalam waktu kurang dari 48-72 jam setelah pasien dirawat ke rumah sakit disebut Healthcare Associated Infection (HAIs) atau biasa dikenal dengan sebutan infeksi nosokomial. Penularan infeksi nosokomial biasanya melalui kontak langsung, droplet, dan melalui tenaga Kesehatan yang ada di rumah sakit, serta melalui alat-alat atau media di rumah sakit. Penelitian ini bertujuan untuk mengetahui pola bakteri dan uji sensitivitas di poli infeksi dan imunologi Rumah Sakit Mata Provinsi Sulawesi Utara. Penelitian ini menggunakan metode deskriptif observasional dengan desain potong lintang untuk mengidentifikasi pola bakteri dan uji sensitivitas di poli infeksi dan imunologi Rumah Sakit Mata Provinsi Sulawesi Utara. Pengambilan 10 sampel dilakukan di poli infeksi dan imunologi. Hasil penelitian mendapatkan empat jenis bakteri yaitu: Staphylococcus sp, Staphylococcus aureus, Bacillus sp, dan Streptococcus sp. Simpulan penelitian ini ialah keempat bakteri ini sensitif dengan meropenem dan resisten dengan erythromycin. Kata kunci:  infeksi nosokomial; identifikasi bakteri; uji sensitivitas
Identifikasi Pola Bakteri dan Uji Sensitivitas Antibiotik di Kamar Bedah Mata Rumah Sakit Mata Provinsi Sulawesi Utara Benjamin, Estelina I.; Homenta, Heriyannis; Waworuntu, Olivia A.
e-CliniC Vol. 12 No. 3 (2024): e-CliniC
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/ecl.v12i3.53622

Abstract

Abstract: Healthcare-associated infections (HAIs) are infections of pathogenic microorganisms into the human body within 48-72 hours after a patient's hospital admission. These infections are commonly attributed to ventilator-associated pneumonia (VAP), surgical site infections (SSI), central line-associated bloodstream infections (CLABSI), and catheter-associated urinary tract infections (CAUTI). Increased HAI cases is also associated with an increase in Multiple Drug-Resistant (MDR) strains due to inappropriate antibiotic selection for treating diseases resulting from HAIs. This study aimed to identify bacterial patterns and sensitivity tests at the ophthalmic surgical room of Rumah Sakit Mata Provinsi Sulawesi Utara. Thus was an observational and descriptive study with a cross-sectional design. The results showed seven Bacillus sp., four Staphylococcus sp., and one Staphylococcus aureus. Sensitivity tests using 18 types of antibiotics revealed antibiotic resistance patterns of the identified bacteria: seven strains of XDR in Bacillus sp., four strains of MDR in Staphylococcus sp., and the presence of Methicillin-Resistant Staphylococcus aureus (MRSA). In conclusion, Bacillus sp. is the most abundant bacteria found. Bacillus sp. is sensitive to the antibiotic meropenem, while it is resistant to ceftriaxone and sulfamethoxazole/trimethoprim. Keywords: healthcare-associated infections; identification of bacteria; antibiotic sensitivity test    Abstrak: Healthcare-associated infections (HAIs) adalah infeksi mikroorganisme patogen ke dalam tubuh manusia yang terjadi 48-72 jam setelah pasien masuk rumah sakit, umumnya disebabkan oleh ventilator-related pneumonia (VAP), surgical site infections (SSI), central line associated blood stream infections (CLABSI), dan catheter-associated urinary tract infections (CAUTI). Peningkatan kasus HAIs sejalan dengan peningkatan multiple drug-resistant (MDR) karena ketidaktepatan pemilihan antibiotik dalam mengobati HAIs. Penelitian ini bertujuan untuk mengidentifikasi pola bakteri dan uji sensitivitas antibiotik di kamar bedah mata Rumah Sakit Mata Provinsi Sulawesi Utara. Jenis penelitian ialah observasional deskriptif dengan desain potong lintang. Hasil penelitian mendapatkan Bacillus sp. tujuh bakteri, Staphylococcus sp. empat bakteri, dan Staphylococcus aureus satu bakteri. Uji sensitivitas terhadap 18 antibiotik menunjukkan gambaran resistensi, yaitu: Bacillus sp. terdapat tujuh bakteri Extensively Drug-Resistant (XDR), Staphylococcus sp. terdapat empat bakteri MDR, dan Methicillin-Resistant Staphylococcus aureus (MRSA). Simpulan penelitian ini, yaitu: Bacillus sp., merupakan bakteri terbanyak yang ditemukan. Bacillus sp. sensitif terhadap antibiotik meropenem namun resisten terhadap antibiotik ceftriaxone, dan sulfamethoxazole/trimethoprim. Kata kunci: healthcare-associated infections; identifikasi bakteri; uji sensitivitas antibiotik