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ANALISIS SENYAWA ANTIBIOTIK DARI JAMUR SIMBION YANG TERDAPAT DALAM ASCIDIANS Didemnum molle DI SEKITAR PERAIRAN BUNAKEN-SULAWESI UTARA Bara, Robert A.; Kandou, Grace D.; Ola, Antonius R. B.; Posangi, Jimmy
JURNAL LPPM BIDANG SAINS DAN TEKNOLOGI Vol 2, No 2 (2015)
Publisher : JURNAL LPPM BIDANG SAINS DAN TEKNOLOGI

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Abstract

Endosymbiont can be interpreted as microbes that live in colonies in the internal tissues of other higher organisms without causing adverse effects directly on the its host. Endosymbiont organisms have a huge potential to be exploited as a new natural products that are useful in medicine, agriculture, and industry. This research is focused on the antibacterial potential. Ascidians Didemnum moll speciments were collected from Manado waters. The fungal isolates were purified followed by screening their antibacterial activity towards Staphylococcus aureus and Pseudomonas aeruginosa. In this study we isolated 3 fungal specimens Acremonium sp. (ADMB-1, 2 and 3). Three isolates showed potential antibacterial activity. Together with the fungal isolates, we also isolated Bacillus galacosidilyticus from the same host. This bacterial isolate also show antibacterial potency.
The Effects of Colostrum Immunoglobulin on Strongyloides Infection in Mice Rumokoy, Laurentius; Posangi, Jimmy; Turangan, Santi; Irianti, Ning; Toar, Wisie Lusia; Aban, Julio Lopez
ANIMAL PRODUCTION Vol 18, No 2 (2016): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (778.936 KB) | DOI: 10.20884/1.anprod.2016.18.2.519

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This experiment was conducted to determine the effects of bovine colostrum IgG on mice infected with Larvae of Strongyloides venezuelensis (L3Sv). This experiment was a 2X2 factorial arrangement including two level of colostrum immunoglobulin-G and two level of endostatine. The parameters were fecal eggs and number of female adult in the gut. The data were statistically analyzed for the mean and standard deviation values, and the differences in treatment groups were subject to ANOVA, if the global differences were detected, a post-ANOVA with LSD test ensued. The development of female adult in the gut, for each group, was performed using Spearman’s rho test. Results showed that colostrum immunoglobulin-G reduce very significantly (P<0.001) the number of eggs per gram feces than in the groups of infected animals treated with endostatine as well as in the mice injected with 3000 L3Vs only. While the reduction of female larvae was significant obtained (P<0.05) in colostrum treatment (L3Sv + colostrum IgG) in comparison with endostatine. It was concluded that the supplementation of immunogobuline-G through the animal digestive system had positively control the Strongyloides venezuelensis parasite infection. This result provides an insight to utilize IgG colostrum in animal husbandry to overcome the threat of parasitic worms infection.
UJI EFEK ANALGESIK EKSTRAK ETANOL DAUN GEDI (Abelmoschus manihot (L.) Medik) PADA MENCIT (Mus musculus) Pratiwi, Ristanti; Posangi, Jimmy; ., Fatimawali
e-Biomedik Vol 1, No 1 (2013): eBiomedik
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/ebm.v1i1.4601

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Abstract: The objectives of the research were to find out the analgesic effect of giving Gedi (Abelmoschus manihot (L.) Medik) leaf ethanol extract orally on the number of writhing after thermal pain induction of mice. This research using 15 mice which is divided into 5 groups consisted of 1 negative control group given by the aquades, 1 positive control group given by the tramadol, and 3 experiment groups. Experiment group given by Gedi (Abelmoschus manihot (L.) Medik) leaf ethanol extract with the doses which different each other, that is 30 mg/30 g BW, 60 mg/30 g BW and 120 mg/30 g BW. Thermal pain induction was done by placing the mice on hot plate constant temperature of 550C. The mice gave respond in the way of lick its foot or even jumping. The data was collected using table, graphic and analyzed using one direction ANOVA model and it was continued with LSD test to find out the difference every treatment group. The result of analysis showed that gedi’s leaf ethanol extract have the analgesic effect and the maximum effect presented at gedi leaf ethanol extract dosage 60 mg/30 g BW. Keywords: Gedi’s leaf, analgesic effect    Abstrak: Tujuan penelitan ini yaitu menemukan efek analgesik dari pemberian ekstrak etanol daun gedi (Abelmoschus manihot (L.) Medik) peroral pada mencit yang kemudian diamati jumlah geliatnya setelah diinduksi panas. Penelitian ini menggunakan 15 ekor mencit yang dibagi 5 kelompok yang terdiri dari 1 kelompok kontrol negatif yang diberi aquades, 1 kelompok kontrol positif yang diberi tramadol, dan 3 kelompok eksperimen. Kelompok eksperimen diberi ekstrak etanol daun gedi dengan dosis yang berbeda-beda, yaitu 30 mg/30 g BB, 60 mg/30 g BB, dan 120 mg/30 g BB. Induksi nyeri berupa panas dilakukan dengan meletakkan mencit pada hot plate dengan suhu 550C . Mencit memberi respon berupa menjilat kaki dan atau melompat. Data disajikan berupa tabel, grafik dan menggunakan analisis statistik ANOVA yang dilanjutkan dengan LSD untuk menemukan perbedaan dari setiap kelompok. Hasil analisis menunjukkan bahwa ekstrak etanol daun gedi memiliki efek analgesik dan efek maksimumnya didapatkan pada dosis 60 mg/30 g BB. Kata kunci: Daun gedi, efek analgesik
UJI EFEK ANTIBAKTERI JAMUR ENDOSIMBION SPONS LAUT Callyspongia sp. TERHADAP BAKTERI Pseudomonas aeruginosa dan Eschericia coli Menggelea, Frengki P.; Posangi, Jimmy; Wowor, Mona P.; Bara, Robert
e-Biomedik Vol 3, No 1 (2015): eBiomedik
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/ebm.v3i1.7410

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Abstract: Callyspongia sp. is one of the kinds of sea sponge which is hardly used instead of its potential in order to be used as antibacterial, anticancer, and also antifungi.The objective of the research is to observe the effect of antibacterial of endosymbiont fungi of Callyspongia sp. to Pseudomonas aeruginosa and Ecshericia coli. Method: Method chosen in order to test the antibacterial effect is by pasting the myselia of endosymbiont fungi on the subject. The result of the combination, then, is observed as a measure of zone of resistance in endosymbiont fungi test effect. Result: The result of the observation presents two kinds of endosymbiont fungi, isolated from the sea sponge Callyspongia; Black Myselium Fungi and Brown Myselium. The diameter’s average of zone of resistance in Brown Myselium Endosymbiont Fungi to Pseudomonas aeruginosa is 16,8 mm; the same amount gained to Eschericia coli, while the Black Myselium presents no zone of resistance. Conclusion: Brown Myselium Endosymbiont Fungi has the ability to resist the growth of Pseudomonas aeruginosa and Eschericia coli.Keywords: antibacterial, endosymbiont fungi, Callyspongia sp. sea sponge.)Abstrak: Callyspongia sp. merupakan salah satu jenis spons yang berpotensi sebagai antibakteri, antikanker, dan antijamur yang belum banyak dimanfaatkan. Tujuan: untuk mengetahui ada tidaknya efek antibakteri jamur endosimbion spons laut Callyspongia sp. terhadap bakteri Pseudomonas aeruginosa dan Eschericia coli. Metode: Metode yang digunakan untuk uji antibakteri yaitu dilakukan dengan cara menempelkan miselia jamur endosimbion pada media agar kombinasi yang telah dioleskan bakteri uji untuk mengamati dan mengukur zona hambat yang dihasilkan jamur endosimbion. Hasil: Dari penelitian yang dilakukan, diperoleh dua jenis jamur endosimbion yang diisolasi dari spons laut Callyspongia sp., jamur endosimbion miselium hitam dan jamur endosimbion miselium coklat. Rata-rata diameter zona hambat jamur endosimbion coklat terhadap bakteri Pseudomonas aeruginosa 16,8 mm dan terhadap Eschericia coli 16,8 mm, sedangkan jamur endosimbion hitam tidak memiliki zona hambat. Simpulan: Jamur endosimbion coklat memiliki aktivitas menghambat pertumbuhan bakteri Pseudomonas aeruginosa dan Eschericia coli.Kata kunci: antibakteri, jamur endosimbion, spons laut Callyspongia sp.
Gambaran dan Rasionalitas Penggunaan Antibiotik pada Pasien Dewasa dengan Community Acquired Pneumonia di RSUP Prof. Dr. R. D. Kandou Manado Periode Juni 2017-Mei 2018 Prakoso, Dimas; Posangi, Jimmy; Nangoy, Edward
eBiomedik Vol 6, No 2 (2018): eBiomedik
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/ebm.6.2.2018.22156

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Abstract: Irrational utilization of antibiotic led to several burdens for healthcare providers, one of them is antibiotic resistance. Community acquired pneumonia (CAP) has increased mortality rate due to irrational antibiotic utilization. This study was aimed to obtain a general depiction and antibiotic rational utilization quantitatively assessed of CAP in adult patients at Prof. Dr. R. D. Kandou Hospital Manado from June 2017 to May 2018. This was a retrospective descriptive study with a cross sectional design. Samples were 42 patients with CAP obtained by using simple random sampling. The results showed that values of DDD/100 inpatient days were, as follows: beta-lactam (33), macrolides (13.758), and fluoroquinolone (20.072). According to the ratio between estimated DDD value of Prof. Dr. R. D. Kandou and DDD WHO, all prescribed antibiotics had DDD values below or close to the value of DDD WHO. Albeit, there were discrepancies between antibiotic utilization in the field and reccomendation of Clinical Practice Guideline of Internal Medicine Department. Conclusion: Within the period of June 2017 - May 2018 the most prescribed antibiotic classes for CAP in adult patients at Prof. Dr. R. D. Kandou Hospital were beta-lactam, macrolides, and fluoroquinolone meanwhile the most prescribed antibiotics were ceftriaxone and azithromycin. In general, drug utilization was rational assessed quantitatively by using DDD WHO criteria.Keywords: antibiotic rationality, CAP, DDD WHO, Prof. Dr. R. D. Kandou Abstrak: Penggunaan antibiotik yang tidak rasional dapat membebani tenaga kesehatan, salah satunya ialah resistensi antibiotik. Community acquired pneumonia (CAP) mengalami peningkatan mortalitas tinggi akibat penggunaan antibiotik yang tidak tepat. Penelitian ini bertujuan untuk mendapatkan gambaran umum dan penilaian rasionalitas secara kuantitatif dari penggunaan antibiotik pada pasien dewasa dengan CAP di RSUP Prof. Dr. R. D. Kandou Manado periode Juni 2017-Mei 2018. Jenis penelitian ialah deskriptif retrospektif dengan desain potong lintang. Sampel sebanyak 41 pasien dewasa dengan CAP diambil dengan simple random sampling. Hasil penelitian mendapatkan DDD/100 hari rawat inap penggunaan antibiotik dari tiga golongan antibiotik yaitu beta-lactam (33), makrolida (13,758), dan florokuinolon (20,072). Berdasarkan rasio estimasi DDD di RSUP Prof. Dr. R. D. Kandou Manado dan DDD WHO seluruh obat yang digunakan masih berada di bawah DDD WHO atau mendekati nilai tersebut. Terdapat perbedaan antara penggunaan antibiotik di lapangan dan rekomendasi dari Panduan Praktek Klinis dari Bagian Ilmu Penyakit Dalam. Simpulan: Pada periode Juni 2017 - Mei 2018 golongan antibiotik yang paling banyak diberikan untuk pasien dewasa dengan CAP di RSUP Prof. Dr. R. D. Kandou Manado ialah beta-lactam, diikuti makrolida dan florokuinolon sedangkan antibiotik yang paling banyak diberikan ialah ceftriaxone dan azithromisin. Secara keseluruhan penggunaan obat sudah rasional secara kuantitatif diukur dengan kriteria DDD WHO.Kata kunci: rasionalitas antibiotik, CAP, DDD WHO, RSUP Prof. Dr. R. D. Kandou Manado
UJI EFEK ANTIBAKTERI JAMUR ENDOFIT DAUN MANGROVE AVICENNIA MARINA TERHADAP BAKTERI UJI STAPHYLOCOCCUS AUREUS DAN SHIGELLA DYSENTERIAE Kasi, Yolanda A.; Posangi, Jimmy; Wowor, O. Mona; Bara, Robert
eBiomedik Vol 3, No 1 (2015): eBiomedik
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/ebm.3.1.2015.6632

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Abstract: Endophytic fungi is a species of fungi which lives within plant tissues including leaf’s tissues that also harmless for the host plant itself. Isolates of endophytic fungi have a potent antibacterial capability with broad spectrum. The aim of this research is to know whether there is an antibacterial effect of endophytic fungi which isolated from Avicennia marina leaf towards Staphylococcus aureus and Shigella dysenteriae. The method that is used for the antibacterial activity test, done by putting mycelia of endophytic fungi in combination media that has been smeared with Staphylococcus aureus and Shigella dysenteriae. Based on research that has been done, it obtained two types of endophytic fungi isolated from mangrove plant, Avicennia marina leaf. Both endophytic fungi have potential for inhibiting Staphylococcus aureus and Shigella dysenteriae, but endophytic fungi with white mycelium has a better antibacterial effect than endophytic fungi with black mycelium and positive control.Keywords: antibacterial, endophytic fungi, mangrove avicennia marinaAbstrak: Jamur endofit merupakan jamur yang hidup di dalam jaringan tanaman salah satunya yaitu dalam jaringan daun dan tidak membahayakan tanaman tersebut. Isolat-isolat jamur endofit memiliki daya antibakteri yang poten dengan spektrum luas. Penelitian ini bertujuan untuk mengetahui ada tidaknya efek antibakteri jamur endofit daun Avicennia marina terhadap bakteri Staphylococcus aureus dan Shigella dysenteriae. Metode yang digunakan untuk uji antibakteri yaitu dilakukan dengan cara menempelkan miselia jamur endofit pada media agar kombinasi yang telah dioleskan bakteri uji. Dari penelitian yang dilakukan, diperoleh dua jenis jamur endofit yang diisolasi dari daun Mangrove Avicennia marina. Kedua jamur endofit memiliki potensi dalam menghambat kedua bakteri uji tetapi jamur endofit miselium putih memiliki efek antibakteri yang lebih baik dibandingkan dengan jamur endofit miselium hitam dan kontrol positif.Kata kunci: antibakteri, jamur endofit, daun avicennia marina
Uji efek antibakteri daun mangrove Rhizophora apiculata terhadap bakteri Pseudomonas aeruginosa dan Staphylococcus aureus Santoso, Vivi P.; Posangi, Jimmy; Awaloei, Henoch; Bara, Robert
e-Biomedik Vol 3, No 1 (2015): eBiomedik
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/ebm.v3i1.7415

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Abstract: Endophytic Fungi are microorganisms that live in the plant tissue systems such as seeds, leaves, flowers, twigs, stems, and roots. Endophytic fungi used in this study was isolated from Mangrove Rhizophora apiculata leaves. R. apiculata is widely grown in coastal areas. This study aimed to determine whether there was an antibacterial effect of endophytic fungi that was isolated from leaves of Rhizophora apiculata against the sample bacteria Pseudomonas aeruginosa and Staphylococcus aureus. This study was conducted in the Pharmacology and Therapeutics Laboratory of Sam Ratulangi University by using experimental methods. The results showed two types of fungi, tusky white endophytic fungi and white filamentous endophytic fungi, isolated from Mangrove Rhizophora apiculata leaves. Both types of fungi showed the presence of bacterial growth inhibition, but the white filamentous endophytic fungi had greater antibacterial effect than the tusky white endophytic fungi.Keywords: endophytic fungi, antibacterial, rhizophora apiculata leavesAbstrak: Jamur endofit merupakan mikroorganisme yang hidup di dalam sistem jaringan tumbuhan seperti biji, daun, bunga, ranting, batang, dan akar. Jamur endofit yang digunakan pada penelitian ini merupakan hasil isolasi dari daun mangrove Rhizophora apiculata. Tanaman mangrove R.apiculata ini banyak tumbuh di kawasan pesisir pantai. Penelitian ini bertujuan untuk mengetahui apakah terdapat efek antibakteri jamur endofit yang diisolasi dari daun Rhizophora apiculata terhadap bakteri uji Pseudomonas aeruginosa dan Staphylococcus aureus. Penelitian ini dilakukan di Laboratorium Farmakologi dan Terapi Universitas Sam Ratulangi Manado dengan menggunakan metode eksperimental. Hasil penelitian ini didapatkan dua jenis jamur yaitu jamur endofit berwarna putih gading dan jamur endofit berwarna putih berserabut yang diisolasi dari daun mangrove Rhizophora apiculata. Kedua jenis jamur menunjukan adanya daya hambat pertumbuhan kedua bakteri uji, namun jamur endofit berwarna putih berserabut memiliki efek antibakteri lebih besar dari pada jamur endofit berwarna putih gading.Kata kunci: jamur endofit, antibakteri, daun rhizophora apiculata
HUBUNGAN PENGETAHUAN PERAWAT DENGAN KEMAMPUAN MELAKUKAN BANTUAN HIDUP DASAR PADA ORANG DEWASA DI INSTALASI GAWAT DARURAT BEDAH RSUP Prof. Dr. R.D. KANDOU MANADO Sandag, Hendra; Posangi, Jimmy; Warouw, Herman
e-NERS Vol 1, No 1
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/ens.v1i1.1785

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Abstract: One indicator of bad good health services is reflected in the language of quality emergency services at installations. Destination of this research was done to know the nurses knowledge  and do get help basic life support  to humans. The design of this research is cross sectional  study. The sample are 40 respondents,the data intake tecnique was by questionnaires and observation directly conducted nurses on midget. The experiment result hipotesis using Fisher’s Exac Test with level of mistake 0,05% and level of confidence 95% so ρ value 0,01 < 0,05, Ha is accepted. Concluded that knowledge nurses nem article ability to get help equity living in emergency in, initial studies were categorized less. Recommendation : Develope knowledge of nursing about getting help during equity life education, required the exercise of the well’s method of analysis laboratory skills tu produce nurses able to perform in sequence and appropriate resucitation. Keywords: Nurse knowledge, Get help with basic life support.     Abstrak: Hendra Sandag, Hubungan pengetahuan Perawat Dengan Kemampuan melakukan Bantuan Hidup Dasar Pada Orang Dewasa Di Instalasi Gawat Darurat Bedah BLU RSUP Prof. dr. R. D. Kandou Manado, Karya Tulis Ilmiah Sarjana, Program Studi Ilmu Keperawatan, Fakultas Kedokteran Universitas Sam Ratulangi. Pembimbing (1) Prof  dr. Jimmmy Posangi,MSc.PhD.SpFK, (2) Herman Warouw, SKM, M.Kep. Salah satu indikator baik atau buruknya pelayanan kesehatan, tercermin dari kualitas pelayanan pada instalasi gawat darurat sebagai gerbang terdepan bagi pasien yang membutuhkan pertolongan kesehatan di institusi rumah sakit tersebut. Penelitian ini bertujuan untuk mengetahui apakah pengetahuan perawat berhubungan dengan bantuan hidup dasar pada orang dewasa. Penelitian ini bersifat cross sectional, Variable independen yaitu pengetahuan perawat dan variable dependen yaitu kemampuan melakukan bantuan hidup dasar. Data didapatkan melalui kuesioner dan observasi secara langsung yang dilakukan perawat pada manikin. Data dianalisa dengan menggunakan uji Fhisher’s Exac Test dengan table 2 x 2 dengan nilai kemaknaan α = 0.05. Hasil uji Fisher’s Exack Test didapatkan nilai P = 0,001 yang berarti lebih kecil dari a = 0,05 dengan demikian H0 ditolak dan Ha diterima, maka dapat dikatakan bahwa ada hubungan yang bermakna antara pengetahuan perawat dengan kemampuan melakukan bantuan hidup dasar pada orang dewasa. Kata kunci: Pengetahuan perawat, Bantuan Hidup Dasar.
HUBUNGAN KUALITAS PELAYANAN KEPERAWATAN DENGAN TINGKAT KEPUASAN PASIEN RAWAT INAP DI IRINA A RSUP PROF DR R D KANDOU MANADO Tiwa, Angela Christin; Posangi, Jimmy; Warouw, Herman
e-NERS Vol 1, No 1
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/ens.v1i1.1769

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Abstract: A service although can be counted satisfy to patient but the aplication do not according to the ethical code and atandart, it is not a quality service. It is, therefore very important the role of  nursing care is very important the affect the patient's satisfaction. The purpose of research know is to the relationship of quality nursing care to the level of satisfaction of hospitalized patients at the department of Irina A Prof Dr R D Kandou Manado. The study design is using observational analytic cross-sectional approach. The Population of the patients who cared at department of Irina A Prof Dr R D Kandou Manado with the average of 180 patients a month. Sixtyvive samples were used in this research . The data is processed by using univariate and bivariate chi square calculations. The results show the relationship of quality nursing care to inpatient satisfaction majority of good quality (58.5%) and poor (41.5%) and to the satisfaction of the majority of the patients were less satisfied (50.8%) and the satisfied (49.2%). As a Conclusion, there is a significant correlation between the quality of nursing care with the patient satisfaction and quality of serviceis assumed as good. As a suggestion the quality of nursing care services of hospital management should be further enhance which can support the profesionalism of the nurses, so the patient satisfaction archieved can be maximized. Keywords: nursing service, patient satisfaction.     Abstrak: Suatu pelayanan sekalipun di nilai dapat memuaskan pasien tetapi penyelenggaraannya tidak sesuai dengan kode etik dan standar bukanlah pelayanan yang bermutu. Oleh karena itu perawat sangat berperan penting dalan pelayanan keperawatan karena berpengaruh dengan kepuasan pasien. Tujuan penelitin mengetahui hubungan kualitas pelayanan keperawatan dengan tingkat kepuasan pasien rawat inap di Irina A RSUP Prof Dr R D Kandou Manado. Desain penelitian observasional analitik dengan pendekatan cross sectional. Populasi seluruh pasien yang berada di ruang rawat inap Irina A RSUP Prof Dr R D Kandou Manado dengan rata-rata pasien per bulan sebanyak 180. Sampel penelitian 65 pasien. Data diolah secara univariat dan bivariat dengan menggunakan perhitungan chi square. Hasil penelitian menunjukkan hubungan kualitas pelayanan keperawatan dengan tingkat kepuasan pasien rawat inap mayoritas kuaitas pelayanan keperawatan yang baik (58,5%) dan yang kurang baik (41,5%) dan untuk kepuasan mayoritas pasien kurang puas (50,8%) dan yang puas (49,2%). Kesimpulan terdapat hubungan  yang bermakna antara kualitas pelayanan keperawatan dengan tingkat kepuasan pasien dan kualitas pelayanannya sudah baik. Saran pihak manajemen rumah sakit hendaknya lebih meningkatkan lagi kualitas pelayanan keperawatan yang dapat menunjang profesionalitas seorang perawat sehingga tingkat kepuasan  pasien bisa maksimal. Kata Kunci: pelayanan keperawatan,  kepuasan pasien.
Profil status karies pada anak usia 13-15 tahun dan kadar fluor air sumur di daerah pesisir pantai dan daerah pegunungan Iswanto, Lidia; Posangi, Jimmy; Mintjelungan, Christy N.
e-GiGi Vol 4, No 2 (2016): e-GiGi
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/eg.4.2.2016.13649

Abstract

Abstract: Dental caries is a destructive process that starts from the enamel to the dentine caused by Streptoccocus mutans bacteria. Fluorine is the most affecting chemical substance to the percentage of dental caries. Residents with geographically different locations have different caries risks. This study aimed to obtain the differences in the status of dental caries in children aged 13-15 years and the fluoride content of well water in the coastal area and mountainous area. This was a descriptive study with a cross sectional design. Samples were 60 people obtained by using purposive non-probability sampling method. Examination of dental caries was carried out by using DMF-T index and fluorine content was measured by using Atomic Absorption Spectrophotometer AAS type A230 with a wavelength of 525 nm. The results showed that the status caries of children aged 13-15 years in the coastal area (Lihunu village) based on DMF-T index was 2.5 (low caries status) meanwhile the status of caries in children aged 13-15 years in the mountainous area (Rurukan village) based on DMF-T index was 6.2 (high caries status). Fluorine content of well water consumed in the coastal village was 0.25 ppm (categorized as very low) and the fluorine content of well water consumed in the rural mountainous area was 0.28 ppm (categorized as very low).Keywords: caries status, fluoride water content, mountainous area, coastal areaAbstrak: Karies gigi merupakan suatu proses kerusakan yang dimulai dari enamel hingga ke dentin yang disebabkan oleh bakteri Streptoccocus mutans. Fluor merupakan unsur kimia yang paling memengaruhi persentase karies gigi. Penduduk yang secara geografis letak kediamannya berbeda memiliki risiko karies yang berbeda. Penelitian ini bertujuan untuk mengetahui perbedaan status karies gigi pada anak usia 13-15 tahun dan kadar fluor air sumur di daerah pesisir pantai dan di daerah pegunungan. Jenis penelitian ini ialah deskriptif dengan desain potong lintang. Pengambilan sampel dengan menggunakan metode purposive non probability sampling dengan sampel sebanyak 60 orang. Pemeriksaan karies gigi menggunakan indeks DMF-T dan pengukuran kadar fluor menggunaan spektrofotometer serapan Atom, AAS tipe A230 dengan panjang gelombang 525 nm. Hasil penelitian status karies pada anak usia 13-15 tahun di daerah pesisir pantai (Desa Lihunu) berdasarkan pemeriksaan indeks DMF-T sebesar 2,5 berada pada status karies rendah dan status karies pada anak usia 13-15 tahun di daerah pegunungan (Kelurahan Rurukan) berdasarkan pemeriksaan indeks DMF-T sebesar 6,2 termasuk dalam status karies tinggi. Kadar fluor air sumur yang dikonsumsi di daerah pesisir pantai sebesar 0,25 ppm (kategori sangat rendah) dan kadar fluor air sumur yang dikonsumsi di daerah pegunungan sebesar 0,28 ppm (kategori sangat rendah).Kata kunci: status karies, kadar fluor air, daerah pegunungan, daerah pesisir pantai
Co-Authors Aaltje E. Manampiring Abdul, Jihan A. Achmad, Mardhita Nilamsari Alamri, Khairun N. H. Amelia I. Toar Anak Agung Istri Sri Wiadnyani Angela Christin Tiwa Angelina Stevany Regina Masengi Antje A Wuwungan Antonius R.B. Ola Arthur Mongan Ayu S. Gugule B H. R. Kairupan Bagaray, Evangelin Fresianly Bato, Donny Christian Bernat S. P. Hutagalung Billy J. Kepel Budhi Setianto Christy N. Mintjelungan Citra R. Irianty Clara, Santi Daniel Febrian Sengkey Dantje T Sembel Dimas Prakoso Dina Rombot Dwilestari . Edward Nangoy, Edward Edward S. Oroh, Edward S. Elisa Mangkey Esther Ariny Rumimper Eva A. Sijabat, Eva A. Faidiban, Aqueline N. Falugah, Fathia Fatimawali , Fatimawali Fatimawali . Febriana ., Febriana Frengki P. Menggelea Go Handayani, Go Gosal, Leonardo Delvin Grace D. Kandau, Grace D. Grace Debbie Kandou Gresty Masi Gustaaf A. E. Ratag Hans Huijbregts Harmani Kalim Hasanuddin, Israyati R. Hendra Sandag Henoch Awaloei Herman Warouw IC Manoppo, Jeanette IC Indriya Robot, Melisa Ira Posangi Jane Wuisan Judo Prihartono Juliatri . Julio Lopez Aban, Julio Lopez Katuuk, Mario Esau Kipimbob, Eflentina Kumala Dewi, Citra Kurnia, Bonar Kurniawan Kurniawan Laurentius Rumokoy, Laurentius Lendombela, Ditya P. J. Lengkong, Gledys Tirsa Lesar, Imelda Lidia Lidia Iswanto, Lidia Linnie Pondaag Lucia I. R. Lefrandt Mallombasang, Andi Nuraini Mamahit, Juliet Merry Eva Mambo, Christi D. Mambo, Christi Diana Manampiring, Aaltje Ellen Manannohas, Mouren Mantjoro, Eva M. Marthen Theogives Lasut, Marthen Theogives Masi, Gresty N.M. Maya Memah Mewengkang, Mario L. Michael A. Leman Minarma Siagian Mona P. Wowor Murdani Abdullah Naray, Gueen L. G. Nas Lokbere Natalia Christine Mantiri Ni Luh G.L. Jayalandri, Ni Luh G.L. Ni Wayan Mariati Nina S Widiarto Ning Irianti, Ning O. Mona Wowor Oddi R Pinontoan Olivia Waworuntu Oroh, Wenda P. Djufri, Moh Akbar P. M. Wowor P. S. Anindita Paliling, Agrianto Pandegirot, Juliana Sisca Pangouw, Excel Paulina yamlean Pemsi M. Wowor, Pemsi M. Pertiwi, Junita Maja Polii, Erfand Priska F. Umboh Putri, Vita A.D Rahadian, Rifky Aditya Ramadi, Reza Pahlevi Rampengan, Nancy Ratag, Gustaaf Regina Masengi, Angelina Stevany Ristanti Pratiwi Robert A. Bara Rondonuwu, Yohanes Amazia Zet Roring, Natalia Megawati Rudianto Tari S. Surya, Welong S. V. Sinolungan, Jehosua Saartje J Lumanauw Salsabila, Febrisa Santi Turangan, Santi Sarwono Waspadji Sirowanto Inneke Sondakh, Refrando M. Starry H. Rampengan Sunny Wangko Surya, Welong S. Suryadi N. N. Tatura, Suryadi N. N. Suryani, Intan Indah Suzanna Immanuel T. Lasut, Markus Tahulending, Jane Tangkuman, Victor Yohanes Tansil, Alberta Y.M. Tarigan, Paulus B. Titi L. Faraknimella, Titi L. Vastelita Lengkong, Injili Anugerah Venesia Pengan VERY LONDA Vivi P. Santoso Vonny N. S. Wowor Vonny Wowor Walewangko, Marfincy Walewangko, Marfincy S. Warouw, Finny Warouw, Inggrid Waworuntu, Waworuntu Welong, Seftian Surya Widya Astuty Lolo, Widya Astuty Wisie Lusia Toar, Wisie Lusia Wulan P. J. Kaunang Y. Tangkuman, Yundi Y. Yolanda A. Kasi Yolanda Bataha Yulianty Sanggelorang