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All Journal Archive of Community Health Buletin Udayana Mengabdi Jurnal Veteriner Prosiding Seminar Nasional MIPA Jurnal Kesehatan Masyarakat Syntax Literate: Jurnal Ilmiah Indonesia Jurnal Gizi Klinik Indonesia Indonesian Journal for Health Sciences (IJHS) Gorontalo Journal of Public Health PREPOTIF : Jurnal Kesehatan Masyarakat PUBLIKASI PENELITIAN TERAPAN DAN KEBIJAKAN MAHESA : Malahayati Health Student Journal Public Health and Preventive Medicine Archive Berita Kedokteran Masyarakat Medika Respati : Jurnal Ilmiah Kesehatan Jurnal Kesehatan Reproduksi Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal) Jurnal Ilmu Kesehatan Masyarakat
Ni Putu Widarini
Program Studi Ilmu Kesehatan Masyarakat, Fakultas Kedokteran Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Dentistry Economics, Econometrics & Finance Education Environmental Science Health Professions Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Journal : Jurnal Veteriner

 1 
CHARACTERIZATION OF LACTIC ACID BACTERIA ISOLATED FROM SUMBAWA MARE MILK Nengah Sujaya; Yan Ramona; Ni Putu Widarini; Ni Putu Suariani; Ni Made Utama Dwipayanti; Komang Ayu Nocianitri; Ni Wayan Nursini
Jurnal Veteriner Vol 9 No 2 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study was carried out to isolate and characterize lactic acid bacteria (LAB) from the Sumbawa mares milk The Isolation of LAB was conducted in Man Rogosa Sharpe (MRS) agar. The isolates were characterized by standard methods, such as Gram staining, cell morphology study and fermentation activities. The ability of the isolates to inhibit some pathogenic bacteria was studied by dual culture assay. Isolates showing the widest spectrum of inhibiting pathogenic bacteria were further identified using API 50 CHL. The results showed that Sumbawa mare milk was dominated by lactobacilli and weisella/leuconostoc. As many as 26 out 36 isolates belong to homofermentative lactobacilli and another 10 isolates belong to both heterofermentative lactobacilli and weissella or leuconostoc. Twenty four isolates inhibited the growth of Escherichia coli 25922, Shigela flexneri, Salmonella typhimurium, and Staphylococcus aureus 29213. Two promising isolates with the widest spectrum of inhibiting pathogenic bacteria, Lactobacillus sp. SKG34 and Lactobacillus sp. SKG49, were identified respectively as Lactobacillus rhamnosus SKG34 and Lactobacillus ramnosus SKG49. These two isolates were specific strains of the sumbawa mare milk and are very potential to be developed as probiotic for human.
PROBIOTIC POTENCY OF LACTOBACILLUS SPP. ISOLATED FROM SUMBAWA MARE MILK I Nengah Sujaya; I Made Utami Dwipayanti; Ni Luh Putu Suariani; Ni Putu Widarini; Komang Ayu Nocianitri; Ni Wayan Nursini
Jurnal Veteriner Vol 9 No 1 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research was deigned to elucidate the potency of Lactobacillus spp. isolated from sumbawa mare milk to be developed as a probiotic. Sixteen lacobacilli were screened based on their resitancy to a model of gastric juice at pH 2, 3, and 4, then followed by their resistncy to small intestional fluid model containing deoxycholic. Three lactobacilli i.e. Lactobacillus sp. SKA13, Lactobacillus rhamnosus SKG34 and Lactobacillus rhamnosus SKG49 were found to be resistentent to gastric juice at pH 3 and 4. However, there were no lactobacilli resisted to pH 2. Lactobacillus rhamnosus SKG34 and Lactobacillus rhamnosus SKG49 were able to reach the colon even after being expossed to a model of intestinal fluid containing 0,4 mM deoxycholate and pancreatine. Therefore, these isolates have a potency to be developed as probiotic lactobacilli. Nevertherless, these lactobcailli could probably transform cholic acid into secondary bile acids, which were not expected to be found in the probiotic, and this capability is not appropriate for probiotic. This character is worthly to be studied since it has never been reported in lactobacilli.
Co-Authors A Swandewi Alex Pangkahila Armini, Ni Luh Gde Dinar Lubis Evita Trisnarini, I Gusti Ayu Fatwa Sari Tetradewi Feni Sulistyawati Feni Sulistyawati Firdawsyi Nuzula Fransiska Nova Nanur Fransiska Nova Nanur I Gusti Ngurah Edi Putra I Nengah Sujaya I Nyoman Mangku Karmaya I Nyoman Mangku Karmaya I Nyoman Mangku Karmaya I.M.S. Adnyana J. A. Pangkahila K Tangking Widarsa Kadek Tresna Adhi Ketut Suarjana KOMANG AYU NOCIANITRI Luh Putu Sinthya Ulandari Luh Seri Ani Luky Adrianto Mahayogi, Ni Putu Tirta Dewi Nandini Parahita Supraba Ni Ketut Sutiari Ni Luh Putu Suariani Ni Luh Putu Suariyani Ni Luh Putu Suariyani Ni Made Utami Dwipayanti Ni Nyoman Deni Witari Ni Nyoman Sudarti Ni Putu Suariani Ni Wayan Budi Astiti Ni Wayan Nursini Nyoman Tri Maryanthi Ova Emilia Pande Putu Januraga Puspitasari, Kadek Dina Putra, I Gusti Ngurah Edi Putri Mariani Putri Mariani, Putri Putu Ayu swandewi astuti Rina Listyowati Rina Listyowati, Rina Sakti, KM. Bima Sawitri, Putu Sri Partini, I Gusti Ngurah Suandana, Iwan Abdi Supraba, Nandini Parahita Yan Ramona