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All Journal Jurnal Kesehatan MPI (Media Pharmaceutica Indonesiana) As-syifaa Jurnal Farmasi Window of Health : Jurnal Kesehatan Batoboh : Jurnal Pengabdian Pada Masyarakat Pharmaceutical Reports Celebes Journal of Community Services MPSJ
Harti Widiastuti
Laboratorium Kimia Farmasi, Universitas Muslim Indonesia, Makassar
Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Uji Aktivitas Antioksidan Minyak Nilam (Pogostemon cablin Benth.) Dengan Metode FRAP Niza, Nabila Ikhwanun; Ahmad, Aktsar Roskiana; Widiastuti, Harti
Makassar Pharmaceutical Science Journal (MPSJ) Vol 3 No 1 (2025)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v3i1.319

Abstract

ABSTRACT Patchouli oil (Pogostemon cablin Benth.) is an essential oil derived from the leaves of the patchouli plant. This oil is recognized for its primary component, patchouli alcohol, an antioxidant. The antioxidant properties of patchouli oil effectively inhibit free radical oxidation through the exchange of hydrogen atoms, forming more stable compounds. This study seeks to evaluate the antioxidant potential of patchouli oil through the FRAP method, utilizing a UV-Vis spectrophotometer for measurement at a wavelength of 692 nm. The findings indicate a regression equation of y = 0.0258x + 0.0256, with correlation coefficient of r = 0.9981 and an antioxidant activity measured at 1.7831 mgQE/g extract. Keywords: Antioxidant; Patchouli oil (Pogostemon cablin Benth.); FRAP method; UV-Vis spectrophotometer ABSTRAK Minyak nilam (Pogostemon cablin Benth.) merupakan minyak atsiri yang diperoleh dari daun tanaman nilam. Minyak ini dikenal memiliki kandungan utama patchouli alcohol, yang berfungsi sebagai antioksidan. Aktivitas antioksidan minyak nilam dapat menghambat oksidasi radikal bebas dengan cara menyumbangkan atom hidrogen, sehingga menghasilkan senyawa yang lebih stabil. Penelitian ini bertujuan untuk mengetahui potensi minyak nilam sebagai antioksidan menggunakan metode FRAP, kemudian diukur menggunakan spektrofotmeter UV-Vis pada panjang gelombang 692 nm. Dari penelitian ini diperoleh persamaan regresi y = 0,0258x + 0,0256, r = 0,9981 dan aktivitas antioksidan sebesar 1,7831 mgQE/g ekstrak. Kata kunci: Antioksidan; Minyak nilam (Pogostemon cablin Benth.); Metode FRAP; Spektrofotometer UV-Vis
Analisis Kandungan Rhodamin B Pada saus Tomat Yang Beredar DiKabupaten Wajo Kecamatan Belawa Menggunakan Metode Spektrofotometri UV-Vis Hudayanti, Andi Isti; Widiastuti, Harti; Suhaenah, Asriani
Makassar Pharmaceutical Science Journal (MPSJ) Vol 1 No 2 (2023)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v1i2.104

Abstract

Rhodamin B is a synthetic dye that is used as a dye for the textile industry which is dangerous. Rhodamin B is often misused as a food and cosmetic coloring agent, where Rhodamin B can cause irritation to the digestive tract, poisoning and can even cause cancer. This study aims to determine the presence or absence of Rhodamin B content in tomato sauce samples circulating in Wajo District, Belawa District. In this study used six samples marked with S1, S2, S3, S4, S5, and S6. Qualitative analysis used the TLC test and quantitatively for positive samples used the Uv-Vis spectrophotometry method. From the research results, it was found that S1, S2, S3, S4, S5 were negative for Rhodamin B while S6 was positive for Rhodamin B where the Rf value was 0.68, the color of the pink stain matched the Rhodamin B comparison.
Analisis Kadar Astaxanthin Ekstrak Cangkang Udang Vannamei (Litopenaeus vannamei) Asal Kecamatan Malangke Barat Dengan Metode Spektrofotometri UV-Vis Afifah, Aulia Nur; Widiastuti, Harti; Muflihunnah, Andi
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 1 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v2i1.132

Abstract

Shrimp waste consisting of heads, shells, legs and tails contains several components such as protein, chitin, enzymes, minerals, carotenoids, and others. One type of carotenoid found in shrimp waste is astaxanthin. Astaxanthin is a carotenoid pigment with higher antioxidant activity compared to other β-carotene and vitamin E derivatives. The purpose of this study was to determine the levels of astaxanthin in the shells of vannamei shrimp (Litopenaeus vannamei) from West Malangke District. The astaxanthin compound contained in vannamei shrimp shells was extracted using the UAE method and liquid-liquid extraction. The percentage value of the reduction is 0.486%. Qualitative analysis used thin layer chromatography (TLC) and quantitative analysis using UV-Vis spectrophotometry. TLC analysis on the sample obtained an Rf value of 0.254. Analysis by UV-Vis spectrophotometry at a maximum wavelength of 203 nm obtained y = 0.1513x – 0.0819, correlation coefficient (r) = 0.9947, Vx0 = 4.64%, and the average level of astaxanthin = 73.2 %.
Analysis of Hydroquinone in Face Whitening Cream Circulating in Pinrang Regency Mustafa, Diyan Nurkhalisah; Aminah, Aminah; Widiastuti, Harti
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 1 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v2i1.172

Abstract

DIYAN NURKHALISAH M. Analysis of Hydroquinone in Face Whitening Cream Circulating in Pinrang Regency. (Supervised by Harti Widiastuti and Aminah). Whitening cream is a cosmetic preparation consisting of a mixture of chemicals and other ingredients that aims to whiten or fade black spots on the skin. Many people use whitening creams because they are sold freely. However, not all whitening creams that are sold freely are safe to use because they contain dangerous ingredients, such as hydroquinone. The purpose of this study was to analyze the hydroquinone compound in whitening cream circulating in Pinrang Regency and to find out how much hydroquinone it contained. Qualitative analysis of the four samples using the Thin Layer Chromatography (TLC) method positively contained hydroquinone which was indicated by the Rf values ​​of the samples and standard standards being the same and the appearance of purple spots which were detected under UV light of 254 nm. Quantitative analysis on four samples using the High Performance Liquid Chromatography (HPLC) method with a flow rate of 1 ml/minute, the mobile phase was methanol:aquabidestilata (55:45), the stationary phase was column C-18, the retention times of the sample and standard were obtained at the same value. namely 1.5 and 1.6 indicating that the sample contains hydroquinone. The linear regression equation y = 39.549x – 283.31 with a correlation coefficient of 0.998. The hydroquinone levels obtained were code A whitening cream of 2.5%; whitening cream B 2.8%; whitening cream C 2.45%; and whitening cream D by 0.9%.
Analisis Kadar Siklamat Pada Selai Yang Beredar Di Perumahan BTP Kota Makassar Dengan Metode Spektrofotometri UV-Vis Andini, Ayu; Widiastuti, Harti; Baits, Muzakkir
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 1 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v2i1.207

Abstract

Sweeteners are chemical compounds that are widely added and used in various processed food and beverage products, one of which is jam. Jam is a semi-solid food made from fruit or other processed products. Sweeteners consist of natural sweeteners and artificial sweeteners. Cyclamate is one of the artificial sweeteners that is often used in society because it has a sweetness intensity 30 times that of pure sugar. Excessive use of cyclamate can cause negative effects on health such as cancer (carcinogenic), nerve disorders, impaired liver function, stomach irritation and changes in cell function. The maximum limit for using cyclamate in jam according to SNI 01-0222-1995 is 2 g/kg. This study aims to identify and determine the levels of cyclamate in jam circulating in BTP Makassar housing. The type of research used is laboratory observation with simple random sampling technique. This research was conducted qualitatively (TLC and precipitation method) and quantitatively (uv-vis spectrophotometric method). The results of this study indicated that all negative samples contained cyclamate with a standard Rf value of 0.875 for cyclamate.
Co-Authors A Amaliah Dahlia ABD. MALIK Abd. Malik Afifah, Aulia Nur Ahmad, Akstar Roskiana Ahmad, Aktsar Roskiana Aminah Aminah Aprianti, Tiara Ardin, Nisradhiyah Caesarika Asriani Suhaenah Ayu Andini Chakrawaty M. Kisan, Chakrawaty M. Hudayanti, Andi Isti Ilyas, Andi Nurjayanti Kamase, Jeni Mamat Pratama, Mamat Muammar Fawwaz Muflihunnah, Andi Mustafa, Diyan Nurkhalisah Muzakkir Baits, Muzakkir Niza, Nabila Ikhwanun Nurmaya Effendi Puspita, Della Rahmawati, Rahmawati Rais Razak, Rais San, Htoo Tint Seniwati Dali St Maryam, St Virsa Handayani Wabula, Rheytno Asdin Zainal Abidin