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Journal : Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering)

Design and Testing of Stream as a Sterilization Tool for Trichoderma sp. Propagation Media Using a Palm Oil Mill Steam Boiler Fajri, Ihsanul; Hartulistiyoso, Edy; Widyastuti, Rahayu
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol 14, No 2 (2025): April 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v14i2.570-581

Abstract

Trichoderma sp. is a fungus used in oil palm plantations to control basal stem rot disease, which can reduce palm oil production by up to 80%. At PT. Bumitama Gunajaya Agro, the production of this fungus uses an autoclave, which can only produce 51 kg of Trichoderma sp. per day per unit. To meet the high demand for Trichoderma sp., a large-capacity sterilization tool called "stream" is needed for mass production. The optimal performance of the Stream shows that effective sterilization can be achieved in 20 min when the tool is operated for 45 min. The effectiveness of this sterilization time is proven by the Total Plate Count (TPC) test, which shows a bacterial count of 109×103 CFU/g and a fungal count of 35 CFU/g. The Trichoderma sp. product produced through this process has a conidium density of 8×108, 100% conidium viability, and an inhibition power of 54%, all of which exceed the standard values of SNI 8027.3:2014. Based on its production capacity, Stream can achieve production of up to 1 ton per day with quality not significantly different from Trichoderma sp. production using an autoclave. Keywords: Autoclave, Basal stem rot disease, Sterilization, TPC, Trichoderma sp.
Design and Testing of Stream as a Sterilization Tool for Trichoderma sp. Propagation Media Using a Palm Oil Mill Steam Boiler Fajri, Ihsanul; Hartulistiyoso, Edy; Widyastuti, Rahayu
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 14 No. 2 (2025): April 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v14i2.570-581

Abstract

Trichoderma sp. is a fungus used in oil palm plantations to control basal stem rot disease, which can reduce palm oil production by up to 80%. At PT. Bumitama Gunajaya Agro, the production of this fungus uses an autoclave, which can only produce 51 kg of Trichoderma sp. per day per unit. To meet the high demand for Trichoderma sp., a large-capacity sterilization tool called "stream" is needed for mass production. The optimal performance of the Stream shows that effective sterilization can be achieved in 20 min when the tool is operated for 45 min. The effectiveness of this sterilization time is proven by the Total Plate Count (TPC) test, which shows a bacterial count of 109×103 CFU/g and a fungal count of 35 CFU/g. The Trichoderma sp. product produced through this process has a conidium density of 8×108, 100% conidium viability, and an inhibition power of 54%, all of which exceed the standard values of SNI 8027.3:2014. Based on its production capacity, Stream can achieve production of up to 1 ton per day with quality not significantly different from Trichoderma sp. production using an autoclave. Keywords: Autoclave, Basal stem rot disease, Sterilization, TPC, Trichoderma sp.
Co-Authors . Anandyawati . Giyanto . Giyanto A M Zuhud, Ervizal Abied Khafidhan Adiyaksa, Ivan Khofian AHMAD JUNAEDI Ana Khalisha Anandyawati, . Anas Iswandi Andreas Panjaitan Andreas Panjaitan Andreas Panjaitan, Andreas Ania Cintaresmini Ania Citraresmini Ania Citraresmini Antonius Suwanto Apsari Putri Wulandari Aris Tri Wahyudi Atang Sutandi Atit Kanti Aviantara, Dwindrata B. Azra Zahrah Nadhirah Ikhwani Bria, Deseriana Budi Nugroho Budi Nugroho Dadang . DADANG DADANG Daniel Happy Putra Darmawan Darwis Delima Napitupulu Delima Napitupulu Devanda Ayu Lidya Permata Putri Dewi Sekar Pengerteni Dewi, Tirta Kumala Dhanti Hanifa Muslimah Dimas Syahiddin DWI ANDREAS SANTOSA Dwi Andreas Santosa Dwi Wulandari Edy Hartulistiyoso Eka Lupitasari Endang Sulistyorini Endang Sulistyorini Enny Widyati Enok Sumarsih ERNIN HIDAYATI Erwinda Erwinda Evi Ambarsari Fahrizal Hazra Fajri, Ihsanul Fany Juliarti Panjaitan Fitriyani, Indri Hapsari Giyanto Gunawan Djajakirana Hariyadi Heru Bagus Pulunggono I Made Sudiana I Nyoman Sujana Ida Widiyawati Idris Idris Idris, Komaruddin Irawati, Arfi Irdika Mansur Iswandi Anas Iswandi Anas Iswandi Anas Iswandi Anas Iswandi Anas Chaniago Jekvy Hendra Jekvy Hendra Joko Warino Kartika, Sari Yulia Khairiyah, Yaumil Laksmita Prima Santi MAIPA DIA PATI Masaru Kobayashi Misbakhul Bait Nabila Dianisa Niken Puspita sari Nofrifaldi Ohiwal, Morgan Priyo Cahyono Putri Wulandari, Dwi Ramadhani Sri Astuti, Andi Febrianti Ratnawati Lantifasari Remila Selvany Risa Rosita Rohani Cinta Badia Ginting Rumella Simarmata Rury Kurniawan Safira Eka Aprianti Sanjaya, Wilhelmus Terang Arga Sari Yulia Kartika Sarjiya Antonius SATRIYAS ILYAS Satya Nugroho Soetanto Abdoellah Sri Sulisawati Suciati, Fuzi Sugeng Santoso Sugiarto, Petrus Sugiyanta , Sukmadewi, Desak Ketut Tristiana Sumarsih, Enok Suryo Wiyono Syaiful Anwar Taufiq Hidayat Tita Puspitasari Titik Tri Wahyuni Umadi, Sarah Sakinah Umi Hidayati Umi Hidayati Utami, Aditya Dyah Widrializa, . Wilhelmus Terang Arga Sanjaya Wilhelmus Terang Arga Sanjaya Wilhelmus Terang Arga Sanjaya Winda Ika Susanti Wiwik Eko Widayati Yayuk R. Suhardjono Yayuk Rahayuningsih Suhardjono Yayuk Rahayuningsih Suhardjono Yuni Lisafitri Yusuf, Sri Malahayati Zuhud, Evrizal A M