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ISOLASI DAN KARAKTERISASI MIKROBA PENGHASIL ANTIBIOTIK DARI RIZOSFER TANAMAN KECIPIR (PSOPHOCARPUS TETRAGONOLOBUS) DAN PISANG (MUSA PARADISIACA) Sari Yulia Kartika Hasibuan; Dori Kusuma Jaya; Paisal Ansiska; Deseriana Bria; Dodi Aprianto
Jurnal Agroteknologi dan Pertanian (JURAGAN) Vol 1 No 2 (2021): JURAGAN (JURNAL AGROTEKNOLOGI DAN PERTANIAN) April 2021
Publisher : LPPM Universitas Bina Insan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32767/juragan.v2i1.45

Abstract

Many microbial populations are found in the rhizosphere, including in the soil layer that follows the plant root system. Rhizosphere soils contain more bacteria, fungi and actinomycetes than non-rhizosphere soils. One of the antibiotic producing bacteria has been isolated from the rhizosphere. The purpose of this study was to isolate and characterize the biochemistry of antibiotic producing bacteria and to evaluate their superiority in inhibiting other types of bacteria. Three microbial isolates were isolated from the rhizosphere of winged bean (K) and banana (P1 and P2) plants. All isolated isolates were tested for antagonism with E. coli, Bacillus, and Pseudomonas bacteria on Nutrient Agar (NA) media to determine antibiotic-producing bacteria. Furthermore, identification and characterization of colony morphology and a series of antagonism and pathogenicity tests were carried out. The three isolates had irregular colony forms (isolates K and P1) as well as oval shapes (isolate P2), flat surface, glossy texture, milky white or cream color, and irregular edges. The antagonism test showed that isolates P1, P2, and K could inhibit the growth of bacterial colonies E. coli, Bacillus, and Pseudomonas, respectively. In the pathogenicity test, the three microbial isolates did not show symptoms of necrosis in tobacco leaves after 2 days of observation.
Study of Oribatids population on three types of land use at PT Nusantara VIII Plantation, Cisarua Sub-district, Bogor, West Java Deseriana Bria; Rahayu Widyastuti; Sugeng Santoso
Journal of Degraded and Mining Lands Management Vol 6, No 2 (2019)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (749.673 KB) | DOI: 10.15243/jdmlm.2019.062.1667

Abstract

Most of the forest area of PT Perkebunan Nusantara VIII have been converted to the plantation (tea, cacao, coffee) and agriculture, such as crops (intercropping of oranges and pineapple) areas. The forest conversion will affect soil biodiversity, which will reduce soil mesofauna population, such as the Oribatids group. Oribatids is one of the soil mesofauna which has a very important role in the soil as a decomposer. Because of its important role and their huge numbers in the soil, Oribatids can be used as a bioindicator of soil fertility. This study aimed to elucidate the abundance and diversity of Oribatids and the relationship between soil properties and population of Oribatids in three types of land use at PT Perkebunan Nusantara VIII. Soil and litter samples were taken in three different types of land use (tea gardens, intercropping, and secondary forests) using a simple random sampling method with a distance of each of the 15 sampling points of 100 m at a soil depth of 0-5 cm. Soil and litter samples were extracted using Berlese Funnel Heat Extractor. Oribatids identification was carried out until the family level, and the Oribatids diversity index were determined according to Shannon's diversity index. The identification results obtained in the three types of land use amounted to 24 families from 172 families found previously. The highest abundance of Oribatids was found in secondary forests both in the soil and litter layer (169.85 and 428.87 individuals/m², respectively), the lowest abundance of Oribatids was found in intercropping both in the soil and litter layer (89.17 and 229.30 individuals/m², respectively). According to Shannon Wiener (H'), the diversity index value ranges from 1.48-1.85 or it was categorized as a low-medium diversity value. The results of this study indicated that there was a positive correlation coefficient value from several families of Oribatids with soil pH, organic C, total N, C/N ratio, water content, and soil temperature.
Isolation and Characterization of Potassium-Solubilizing Bacteria from Two Different Rhizospheres and a Cow Manure in IPB University Dori Kusuma Jaya; Sari Yulia Kartika Hasibuan; Deseriana Bria
Jurnal Biologi Tropis Vol. 21 No. 2 (2021): Mei - Agustus
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i2.2559

Abstract

Applying PGPB (Plant Growth-Promoting Bacteria) as bioinoculant under the excessive and continuous use of chemical fertilizer, pesticide, and herbicide need to be highlighted nowadays. One of the most beneficial PGPB is potassium-solubilizing rhizobacteria. Potassium is one of the elements that plant absorbs in a large amounts along with nitrogen. Soil in various regions of Indonesia have low potassium solubility. Therefore, finding the best novel isolates contributing to potassium solubilization need to be carried out continually. The aim of this study is to find potassium-solubilizing bacteria from two different rhizospheres (banana and chili plant) in Leuwikopo Experimental Garden and a cow manure in Cattle Pen, IPB University. To obtain the potassium-solubilizing isolates, we observed and characterized the best growing isolates and measured the solubilizing zone on Alexandrov agar medium. Three out of 10 isolates were selected and tested their pathogenicity on tobacco leave. Solubilization index of three isolates from the highest to the lowest were MPK P (3.07), MPK KT (3.03), and MPK C (2.63), respectively. Necrotic on tobacco leave after isolates treatment showed negative result which means that the three isolates were not pathogenic to plant host. The isolates are recommended to be used as bioinoculant both in greenhouse and field plants which lack of potassium availability in soil.
Pemanfaatan Lahan Pekarangan untuk Budidaya Pakcoy Sistem Vertikultur pada KWT Mawar di Desa Kuaken Kabupaten Timor Tengah Utara Krisantus Tri Pambudi Raharjo; Wilda Lumban Tobing; Boanerges Putra Sipayung; Asep Ikhsan Gumelar; Deseriana Bria; Marinus Kofi; Adolfianus Nino; Imanuel Seran
PRIMA: Journal of Community Empowering and Services Vol 6, No 1 (2022): June
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/prima.v6i1.54029

Abstract

Utilization of Yard Land for Pakcoy Cultivation Verticulture System at KWT Mawar in Kuaken Village Timor Tengah Utara Regency. Mawar’s Woman Farmers Group (KWT) is located in Kuaken Village and has dry land with shallow and rocky solum. One of the businesses of KWT Mawar is cultivating vegetables. However, the land condition, which is quite dry and far from home, makes it difficult to cultivate crops, and they left her job as a housewife. The yard of Mawar’s KWT can be more productive for growing vegetables through the verticulture system. This service aims to utilize the yard as a pakcoy cultivation area with a vertical system using cow manure and biochar as a planting media at KWT Mawar, Kuaken Village. The activities were carried out in the KWT Mawar in June-August 2021. The activities consisted of several stages, namely: 1) verticulture pipe preparation, 2) planting media preparation, 3) seed sowing, 4) planting, and 5) harvesting. The result showed that the use of yardland through pakcoy cultivation with a vertical system provides many benefits for the community compared to cultivation in beds. This activity resulted in an average plant weight of 80.66 g/hole so total production of about 63 kg in 1 harvest was obtained. The productivity was 1.05 kg/paralon pipe. In addition, these activities have created food resiliences for the family, easier to carry out other activities as housewives, saved time and energy, can process rice husk and cow dung waste for a mixture of planting media, and made the yard have aesthetic value.
Pengaruh Jenis dan Konsentrasi Teh Kompos terhadap Pertumbuhan dan Hasil Bayam Merah (Alternanthera amoena Voss) Deseriana Bria
Savana Cendana Vol 1 No 03 (2016): Savana Cendana (SC) - July 2016
Publisher : Fakultas Pertanian, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (569.209 KB) | DOI: 10.32938/sc.v1i03.56

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh jenis dan konsentrasi teh kompos terhadap pertumbuhan dan hasil bayam merah (Alternanthera amoena, Voss) varietas Red R. Dua jenis teh kompos, guano dan campuran arang sekam+hijauan, diberikan dalam konsentrasi 100, 50 dan 25% pada bayam merah dalam sebuah percobaan pot yang disusun menurut rancangan acak lengkap faktorial dengan tiga ulangan. Teh kompos diberikan setiap minggu semenjak tanaman berumur 14 hingga 35 hari setelah tanam. Hasil penelitian menunjukkan bahwa aplikasi teh kompos meningkatkan jumlah daun 106,9-126,3% dan hasil (berat segar total) bayam merah 136,6-237,3% dibandingkan dengan kontrol. Hasil tertinggi bayam merah yang diekspresikan dalam bentuk berat segar per tanaman yakni 6,81 g atau 2,4 kali lipat dibandingkan dengan kontrol diperoleh dari aplikasi teh kompos guano 25%. Hasil yang serupa diperoleh dari pemberian teh kompos arang sekam + hijauan 50%.
Pengaruh Pemberian Mikroorganisme Lokal (MOL) dari Bonggol Pisang terhadap Pertumbuhan dan Hasil Tanaman Bawang Putih Lokal Eban (Allium Sativum L.) Deseriana Bria; Virginia Luruk Seran
Savana Cendana Vol 7 No 03 (2022): Savana Cendana (SC) - July 2022
Publisher : Fakultas Pertanian, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (552.84 KB) | DOI: 10.32938/sc.v7i03.1881

Abstract

This study aims to determine the effect of local microorganism (mol) on the growth and yield of garlic (Allium Sativum L.). The method used is a Randomized Block Design (RAK), namely Concentration (K) which consists of three levels, namely Control (No Treatment), Concentration 1:4 (K1), Concentration 1:5 (K2). The second factor is Watering Frequency (F) which consists of three levels, namely once every three weeks (F1), every five weeks (F2), and once every seven weeks (F3). The results of the study concluded that the 1:4 banana weevil mole concentration treatments were the best treatment in increasing the growth and yield of garlic plants. This can be seen from the highest value of the parameters of the number of leaves, fresh weight of tubers, dry weight of tubers, number of tubers per plant
Soil Characterization In Ex-Manganese Mining Land In North-Central Timor District, East Nusa Tenggara Wilda Lumban Tobing Tobing; Maria Magdalena Kolo; Deseriana Bria; Mahardika Putra Purba; Efieno Maria Paulo Soares
International Journal of Science, Technology & Management Vol. 3 No. 6 (2022): November 2022
Publisher : Publisher Cv. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v3i6.631

Abstract

Manganese (Mn) mining in North Central Timor results in open land conditions that can change soil qualities. The aim of this study was to determine physical characteristics and chemical properties of the soil in the ex-manganese mining areas. The study was performed from June – September 2021. Soil characterization was carried out at selected locations where manganese mining was carried out both traditionally and using technology. The soil characteristics measured were texture, water content, bulk density, mass density, porosity, organic matter content, pH, cation exchange capacity (CEC), organic C, total nitrogen (N), total phosphorus (P), total potassium (K), and total Mn. The results showed that the physical characteristics of the soil on ex-Mn mining land from 5 villages in TTU, NTT are dominated by sand texture which results in low water content (3.39 - 9.46 %), soil porosity (14.96 - 59.31 %), and high bulk density (1.18 - 1.49 g cm-3). Characteristics of soil chemical properties showed low pH (< 7), organic matter (0.06 – 0.81%), organic C (0.03 – 0.22 %), total N contents (0.05 – 0.22%), and high total P (14.77 – 61.44 me 100 g-), K (66.66 – 71.18 me 100 g-), and Mn (18.802 – 24.055 ppm) contents. Land must be reclaimed Reclamation can be done by land management, top soil conservation, planting pioneer crops or cover crops.
PENINGKATAN KETAHANAN PANGAN DI LAHAN KERING PADA KELOMPOK TANI PEREMPUAN SION MELALUI PENERAPAN PEKARANGAN LESTARI Boanerges Putra Sipayung; Wilda Lumban Tobing; Deseriana Bria; Natalia Desy Djata Ndua; Kristoforus Wilson Kia; Eduardus Yosef Neonbeni; Azor Yulianus Tefa; Fransiskus Yulius Dhewa Kadju; Lukas Pardosi; Risna Erni Yati Adu
Abdimas Galuh Vol 5, No 2 (2023): September 2023
Publisher : Universitas Galuh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25157/ag.v5i2.11117

Abstract

Kelompok tani Perempuan Sion aktif dalam kegiatan bercocok tanam di lahan kering. Namun terdapat kesulitan dalam mengelola lahannya. Tujuan pengabdian ini adalah meningkatkan ketahanan pangan kelompok tani Perempuan Sion melalui penerapan pekarangan lestari. Pelaksanaan dilakukan melalui sistem learning by doing dengan sosialisasi dan demonstrasi yang melibatkan kelompok tani Perempuan Sion dan mahasiswa sebagai implementasi MBKM. Kegiatan berlangsung dengan durasi 3 (tiga) bulan mulai dari Juli – September 2022. Tahapan kegiatan dimulai dari persiapan lahan, pembuatan pupuk bokashi berbahan dasar limbah dan kotoran ayam, persemaian benih pakcoy, pemupukan, penanaman, perawatan tanaman, dan pemanenan. Berdasarkan hasil kegiatan pengabdian, kelompok tani Perempuan Sion mampu mengubah limbah menjadi bokashi, mampu mengelola lahan kering sebagai lahan pertanian yang lebih efektif dan efisien baik secara waktu dan tenaga, serta mampu meningkatkan ketahanan pangan.
Pertumbuhan dan Hasil Kandungan Fe dan Zn pada Beras dari Kultivar Padi Ladang di Kabupaten Timor Tengah Utara Asep Ikhsan Gumelar; Florian Mayesti Prima R. Makin; Deseriana Bria
Paspalum: Jurnal Ilmiah Pertanian Vol 11, No 1 (2023)
Publisher : Lembaga Penelitian dan Pengabdian Universitas Winaya Mukti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35138/paspalum.v11i1.480

Abstract

Field rice (paddy field) is one of the important crops/commodities and is an integral part of the dry land farming system in North Central Timor district. The content of rice is to meet the needs of carbohydrates in the body. The materials used are harvested grain from the local North Central Timor rice cultivar which has been carried out for the 2022 planting season. A total of 6 field rice in North Central Timor district were to be tested for Fe and Zn content. Grain samples were taken from crops grown in the same season and location, namely in the dry season of 2022. This study aims to identify local Timorese rice varieties that have high and stable Fe and Zn contents. The study was conducted in the Field Laboratory of the Faculty of Agriculture, University of Timor with a non-factorial Randomized Block Design consisting of 6 cultivar treatments V1 V2 V3 V4 V5 and V6 and the XRF Laboratory, BB Padi Sukamandi. Test the content of Fe and Zn using an XRF machine with an x-ray based performance system. A total of 6 types of field rice were tested for the content of Fe and Zn in brown and polished rice and repeated in duplicate. The content of Fe and Zn is expressed in units of ppm. Data processing was carried out using Excel and SAS 9 software. The results showed that there were differences in the growth and production components between the local Timor and East Timor upland rice cultivars. Cultivars with cultivar V2 (muit fua naek) productivity as seen from the t/ha yield of 3.20 tons per ha were significantly higher than the other cultivars. The results of the analysis of Fe and Zn content showed that cultivar V6 (Aen Mael) had the highest Fe content of 15.1 among the other cultivars, and the lowest was cultivar V4 (Aen Ek ELo) which had an Fe content of 13.0. The highest Zn content was in cultivar V5 (Aen Muit Fua Ana) with a Zn content of 27.9 while the lowest was in cultivar V2 (Muit Fua Naek) with a Zn content of 20.4.
Penerapan Pekarangan Pangan Lestari Melalui Vertikultur Pada KWT Sejati Kelurahan Aplasi, Nusa Tenggara Timur Wilda Lumban Tobing*; Hyldegardis Naisali; Achmad Subchiandi Maulana; Boanerges Putra Sipayung; Kristoforus Wilson Kia; Deseriana Bria; Misail Epainetus Silla; Veronika Sofia Jaquelin Talan
Dinamisia : Jurnal Pengabdian Kepada Masyarakat Vol. 8 No. 1 (2024): Dinamisia: Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/dinamisia.v8i1.16492

Abstract

Fulfillment of food can be done through the implementation of Sustainable Food Yards. KWT Sejati has difficulty providing food throughout the year. The yard owned is not productive because it has dry land biophysics with a dry climate. This condition is also a factor that influences people's food status (food insecurity). Management of dry land as agricultural land requires the adoption of technology. Verticulture technology can be used as an alternative to meet food needs in Karing. This activity aims to apply P2L to KWT Sejati through verticulture as a technology for cultivating vegetables on dry land. The method for implementing this activity is counseling and demonstration using a learning by doing system. The results of this activity provide many benefits to KWT Sejati, including: ease in managing dry land as agricultural land, being able to engineer planting media to improve soil quality, and producing higher production and income compared to using a raised bed system, and has the potential to overcome food insecurity conditions