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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Anestesiologi Indonesia Jurnal Perlindungan Tanaman Indonesia BERITA BIOLOGI JURNAL BIOLOGI INDONESIA JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ANNALES BOGORIENSES Jurnal Entomologi Indonesia Annales Bogorienses
Witjaksono Witjaksono
Fakultas Pertanian Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Journal : BERITA BIOLOGI

 1 
INDUKSI BIAK KALUS DAN BIAK SUSPENSI SEL Aquilaria malaccensis Lam. [Induction of Callus Culture and Cell Suspension Culture of Aquilaria malaccensis Lam.] Leksonowati, Aryani; Witjaksono, Witjaksono; Ratnadewi, Diah
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2672.281 KB) | DOI: 10.14203/beritabiologi.v16i1.2687

Abstract

Aquilaria malaccensis Lam. is a plant species producing fragrant woody material that contains some resin. The compounds can be used as medicine and perfume. Sesquiterpenoid, one group of compounds has been found being synthesized and subsequently extracted from callus and cell suspension culture of Aquilaria species. The aim of this research was to find a method of producing friable calli and cell suspension cultures from leaves or internodes of A. malaccensis in vitro by using suitable plant growth regulators; cell suspension that will suitably serve as material to produce sesquiterpenoid afterwards. Calli were established in almost all treatments of auxin-cytokinin on both leaves and internod explants. The treatment of 10 mg/L IBA induced the highest percentage of callus coverage from leaves with a rather compact structure. The combined treatment of 1–2 mg/L 2.4-D and 0.2–0.3 mg/L BA induced friable callus formation in more than 80% of cultures with 27–32% callus coverage percentage.  The use of 2,4-D induced a better formation of cell suspension than Picloram, with maximum volume up to 7 mL. Cell suspension culture with fine and homogenous aggregate could be established in the medium supplemented with 0.5 –1 mg/L 2,4-D.
KARAKTERISASI PISANG REJANG TETRAPLOID HASIL INDUKSI DENGAN ORYZALIN [Characterization of tetraploid Pisang Rejang induced by oryzalin] Poerba, Yuyu Suryasari; Handayani, T; Witjaksono, Witjaksono
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3022.357 KB) | DOI: 10.14203/beritabiologi.v16i1.2210

Abstract

Triploid banana cultivar is the most desirable cultivar in banana industry because of its higher yield compared to its diploid cultivar.  The  triploid cultivar can be produced by crossing tetraploid with diploid cultivar.  However, tetraploid banana cultivar is rarely existed naturally.  Induced tetraploid of Pisang Rejang was produced using oryzalin. The present research was conducted to characterize tetraploid Pisang Rejang (Musa acuminata, AAAA genome) induced by in-vitro oryzalin treatment from diploid Pisang Rejang. Ploidy level, molecular and morphotaxonomic characters were observed.  Ploidy identification of induced Pisang Rejang was conducted using Flowcytometer.  Molecular characterization was done using RAPD and ISSR markers. Morphology characters were observed based on UPOV (2010). The results showed that tetraploid plants have similar genetic properties with their diploid controls as shown by genetic identity of 0.9901 – 0.9935. The tetraploids were differed from their diploid plants in plan habit and diameter of fruit.  The tetraploid plants produce fewer suckers, drooping leaves and broader fruits compared to its diploid control.
INDUKSI KALUS DAN ORGANOGENESIS KULTUR IN VITRO Dendrobium lineale Rolfe Hoesen, Djadja Siti Hazar; Witjaksono, Witjaksono; Sukamto, LA
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i3.790

Abstract

Callus induction and organogenesis were evaluated in the protocorm like bodies (PLB) culture of Dendrobium lineale Rolfe (Orchidaceae). The globular PLB were cultured in Murashige and Skoog (MS) medium with half strength macronutrients QA MS), supplemented with thidiazuron (TDZ 0,1, 0,5 and 1) mg/1 and 2,4 Dichlorophenoxy acetic acid (2,4-D 0.1, 0.5, 1 and 5) mg/1 as treatments. The media containing 1 mg/1 TDZ and (1 and 5) mg/1 2,4-D was the best treatment for callus initiation (100%). The largest diameter of callus was obtained from 1 mg/1 TDZ (average 3.5 cm). Shoot buds regeneration achieved on 1 mg/1 TDZ (average 41.66 %). However roots regeneration was very low (average 6.66 %) obtained from 1 mg/1 2,4-D. The number of adventitious buds produced from the regenerated shoots on media without plant growth regulator
PERBANYAKAN ULIN (Eusideroxylon zwageri T.et.B) DENGAN BUI DAN SETEK Utami, Ning Wikan; Hoesen, Djadja Siti Hazar; Witjaksono, Witjaksono; Danu, Danu
BERITA BIOLOGI Vol 7, No 4 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (519.393 KB) | DOI: 10.14203/beritabiologi.v7i4.2064

Abstract

The ulin or so called vernacular name kayu besi (Eusideroxylon zwageri T.et.B.) is one of the the important timber in East Kalimantan (East Borneo).However, there are some problems in the regeneration of this species. The research on propagation of ulin was carried out in Treub Laboratory, Research Center for Biology. The research consist of two experiments The research consisted of two experiments.The first experiment seeds which was treated in order to stimulate the germination and the second experiment by treating the mixture between 1BA and vitamin C for stimulating root cutting.The best result of seeds germination was recorded 90%,which the seeds were treated by removing the whole seedcoat and then by exposing to the sunlight for 5 hours The seed itself began to germinate 7 weeks after sowing.Meanwhile, the control no germination et all until 28 weeks.The best result on cutting was indicated by treating with the combination of IBA 10 mg/1 and vitamin C 50 mg/1 which was achieved the highest rooting 100%,although without hormone treatment only resulted 33.3% rooting.
Co-Authors Akbar, Muhammad Aulia Aldawood, Abdulrahman Saad Andriyanto, Rofiq Aria Dian Primatika Arman Wijonarko Ayuningtias, Nandini Azzahra, Zulfa Dani Satyawan Danu Danu, Danu Diah Ratnadewi Diyah Martanti, Diyah Djadja Siti Hazar Hoesen Dyah, Rosliana Purwaning Edhi Martono Estiati, Amy Estiati, Amy F. X. Wagiman, F. X. FAJARUDIN AHMAD HABIB RIJZAANI Handayani, T Harahap, Nafsiyah Agustina I Made Tasma Izzati, Dian Nurul Kiki Yolanda, Kiki Kristamtini Kristamtini, Kristamtini KUNTO WIBISONO Laksono Trisnantoro Leksonowati, Aryani Leksonowati, Aryani Leksonowati, Aryani Leksonowati, Aryani Lukman Hakim Mastur Mastur Nena, Ade Nena, Ade Ning Wikan Utami Nugraheni, K. Utami Nugraheni, K. Utami Nugroho Susetya Putra Permada, Johan PUJI LESTARI Purwanto, Oky Dwi Rahmi, Putri Ridwan, Ridwan Soffan, Alan Subiadi Subiadi Suhendar, Ace Sukamto, LA Sumarno . Suparjo Tanjung, Heru Rezki Tinon Anindita Tri Handayani Tri Harjaka, Tri Umar Umar Utari, Ratna Y. Andi Trisyono Yosefus F. da-Lopez Yuyu S. Poerba, Yuyu S. Yuyu Suryasari Poerba