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All Journal JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI International Journal of Engineering, Science and Information Technology EduBiologia: Biological Science and Education Journal Life Science Aceh International Journal of Science and Technology Prosiding Seminar Nasional Pemberdayaan Masyarakat (SENDAMAS)
Syafitri Jumianto
Univeritas Al-Azhar Indonesia
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Physics Public Health Social Sciences Transportation

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Early Detection of Escherichia coli Producing Extended Spectrum Beta Lactamase (ESBL) in Cattle Farm Waste from Urban Jakarta Aulia Maulida; Syafitri Jumianto; Riris Lindiawati Puspitasari, S.Si., M.Si
Prosiding Seminar Nasional Pemberdayaan Masyarakat (SENDAMAS) Vol 5, No 1 (2025): Desember 2025
Publisher : UniversitasAl Azhar Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36722/psn.v5i1.5236

Abstract

This study examined the presence of Escherichia coli resistant to third-generation cephalosporins in cattle manure originating from an urban farming area in South Jakarta. The study aimed to detect presumptive extended-spectrum beta-lactamase (ESBL)-producing E. coli using total plate count analysis, selective culture, Gram staining, and biochemical identification. Urine and fecal waste samples were collected from a cattle farm located in close proximity to residential settlements. Samples were cultured on MacConkey Agar supplemented with cefotaxime, followed by Gram staining and IMViC biochemical tests. All isolates showed lactose-fermenting colonies with pink to red coloration on MacConkey Agar, Gram-negative rod morphology, and biochemical characteristics consistent with E. coli (Indole positive, Methyl Red positive, Voges–Proskauer negative, Citrate negative). Total bacterial counts reached approximately 10³ colony-forming units per milliliter, with fecal samples exhibiting higher bacterial loads than urine samples. Bacterial growth on cefotaxime-supplemented media indicated phenotypic resistance to third-generation cephalosporins, suggesting the presence of presumptive ESBL-producing strains. These findings demonstrate that urban cattle manure may serve as a reservoir of antibiotic-resistant bacteria and highlight the need for confirmatory ESBL testing, strengthened antimicrobial resistance surveillance, and the implementation of integrated One Health strategies to mitigate environmental and public health risks.Keywords – Antimicrobial Resistance; Cattle Farm Waste; Cephalosporin Resistance; Escherichia Coli; Urban Livestock.
Empowering Students in Maggot Cultivation at the Jamiyyatul Mubtadi Cibayawak Islamic Boarding School Syafitri Jumianto; Lusi Anindia Rahmawati; Asep Maksum; Musoffa Musoffa; Aris Machmud
International Journal of Engineering, Science and Information Technology Vol 5, No 4 (2025)
Publisher : Malikussaleh University, Aceh, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52088/ijesty.v5i4.1807

Abstract

Organic waste management remains a critical global challenge, particularly in developing countries where improper handling contributes to environmental degradation and public health risks. In Indonesia, the dominance of organic waste in landfills presents an opportunity for circular economy practices. This study aims to examine the effectiveness of maggot (Black Soldier Fly/BSF) cultivation as a community-based empowerment strategy to enhance waste management, improve student nutrition, and strengthen economic resilience within an Islamic boarding school environment. This research employed a participatory community-based approach involving 300 students at the Jamiyyatul Mubtadi Cibayawak Islamic Boarding School. The intervention consisted of five main stages: preparation and coordination; socialisation and team formation; technical and managerial training; implementation of appropriate technology; and continuous mentoring and evaluation. Organic waste generated from the school kitchen (approximately 200 kg/day) was partially processed (30–50 kg/day) into maggot biomass, which was subsequently utilised as feed for catfish cultivation. Data were collected through observation, production records, and pre- and post-intervention assessments. The findings demonstrate significant improvements across multiple dimensions. Maggot production increased from approximately 0.5 kg/day to 1.6 kg/day following enhanced waste utilisation and the introduction of supporting technologies such as drying and pelletizing machines. Student participation expanded from 5 to 20 active members in the santripreneur group. Nutritional outcomes also improved, as indicated by increased frequency of fish consumption from once to twice per week and a rise in nutritional knowledge scores from 56% to 82%. Additionally, maggot-based feed production improved in quality, achieving a longer shelf life (up to 3 months) and greater efficiency in aquaculture practices, with catfish survival rates exceeding 80%. The study highlights that integrating maggot cultivation with waste management and aquaculture can effectively support a sustainable circular economy model in educational institutions. Beyond environmental benefits, this approach contributes to improved food security, reduced feed costs (up to 40%), and the development of entrepreneurial skills among students. Despite initial constraints in technology and management capacity, targeted training and infrastructure support proved essential in optimising outcomes. This model demonstrates strong potential for scalability and replication in similar community-based settings.
Co-Authors Ahmad, Abdul Kadir Asep Maksum Aulia Maulida Dewi Elfidasari Gayatri Atmadi Hidayat Yorianta Sasaerila Imsar Gunawan Iskandar, S Jaceyntha Sarah Kesi Kurnia Kesi Kurnia, Kesi Kun Mardiwati Rahayu Lubis, Syakira Kiyasa Lusi Anindia Rahmawati Machmud, Aris Maksum, Asep Muh Asrul Irawan, Andi Mujadin, Anwar Musoffa Musoffa Nina Anindyawati Nina Nina Hermayani Sadi NURUL HIDAYAH Nurul Robbi Sepang Riris Lindiawati Puspitasari Wijihastuti, Risa Swandari Yunus Effendi Zakia Umami