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All Journal Jurnal Online Mahasiswa (JOM) Bidang Keguruan dan Ilmu Pendidikan Nexus Kedokteran Klinik Nexus Biomedika Nexus Kedokteran Translasional Berkala Ilmu Kesehatan Kulit dan Kelamin GIZI INDONESIA Jurnal JUMPED (Jurnal Manajemen Pendidikan) Jurnal Ilmu dan Teknologi Kesehatan Idea : Jurnal Studi Jepang EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi Al-Mutharahah: Jurnal Penelitian dan Kajian Sosial Keagamaan Electoral Governance : Jurnal Tata Kelola Pemilu Indonesia Smart Society Empowerment Journal JURNAL ILMIAH EKONOMI, MANAJEMEN, BISNIS DAN AKUNTANSI JPMFKI
Yulia Sari
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Pengaruh Pemberian Ekstrak Etanol Bit Merah (Beta vulgaris) terhadap Kerusakan Histologis Sel Ginjal Mencit (Mus musculus) yang Diinduksi Parasetamol Amanda Yessica Anggara; . Muthmainah; Yulia Sari
Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Kidney failure can happen due to intoxication of paracetamol that causes oxidative stress. Red beet (Beta vulgaris) containing various components such as vitamin A, C and E, flavonoid, Cu, Zn, Mn, selenium, betanin, and phenolic has a function as antioxidants. The aims of this research were to know the nefroprotective effect of ethanol extract of red beet to prevent renal cell damage of mice. Methods: This was laboratory experimental research with posttest only controlled group design. Subjects of this research were thirty Swiss webster male mice (Mus musculus), 2-3 months old and 20 grams of body weight (BW), each.. The subjects were divided into 5 groups randomly. The groups of this research were negative control (KK(-)), positive control (KK(+)), experimental group 1 (KP1), experimental group 2 (KP2), and experimental group 3 (KP3). The KP1, KP2 and KP3 were administered 5.6 mg, 11.2 mg and 22.4 mg per 20g BW, successively. The nefroprotective effect of ethanol extract of red beet to prevent renal cell damage of mice was assessed by counting the number of damage cells among 50 renal cells on pars convulata. Results: One-Way ANOVA analysis p=0.000 (p<0.05) followed by Post Hoc Multiple Comparisons showed a significant difference between negative control and other groups. The increasing dose of the ethanol extract of red beet was followed by the improvement of the protective effect. The linear regression test p=0.000 (p<0.05) showed a correlation between the increase of dose of ethanol extract of red beet and the improvement of the nefroprotective effect to the histological damage of mices renal cells. Conclusions: Ethanol extract of red beet showed the nefroprotective effect to the histological damage of mices renal cells induced by paracetamol. The increase of dose of the ethanol extract of red beet improved the protective effect to mices renal cells. Keywords: ethanol extract of red beet, histological damage of mices renal cells, paracetamol
Pengaruh Ekstrak Etanol Daun Kenikir (Cosmos caudatus Kunth.) terhadap Kerusakan Struktur Histologis Ginjal Mencit (Mus musculus L.) yang Diinduksi Parasetamol Lina Kristanti Wibowo; . Suyatmi; Yulia Sari
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Kenikir leaves contain various kind of antioxidants, such as vitamin C, ?-carotene, minerals, and polyphenols which are known to protect kidneys from oxidative stress caused by free radical exposure. The purpose of this study is to evaluate the nephroprotective effect of Kenikir leaf extract toward histological damage of the mice's kidney cells induced by Parasetamol. Methods: This was a laboratory experimental study with the posttest only controlled group design. The samples were 35-Swiss webster male mice, aged 2-3 months, and weight about 20 gram. Samples were divided into five groups randomly. Normal group (KN) was given distilled water and Negative control (KK(-)) was given Parasetamol. Extract dosage of 2.8 mg, 5.6 mg, and 11.2 mg of kenikir leaves were given daily for 14 days to treatment group 1 (KP1), treatmen group 2 (KP2), and treatment group 3 (KP3), respectively. A toxic dosage of Parasetamol as much as 5.07 mg, was given to negative control group and treatment groups on day 12th, 13th, and 14th. On day 15th , mice were sacrificed by neck dislocation. The kidneys were taken for histological preparation and stained with HE. The histological damage was assessed based on quantifying the pyknosis, karyorrhexis, and karyolysis on 50 proximal tubuli of epithelial cells. The data were analyzed using One-way ANOVA test (? = 0.05) and Post Hoc Multiple Comparisons test (? = 0.05). Results: The most damaged cells were found in KK(-) and the least in KN. The significant difference was observed among groups (One-way ANOVA test p = 0.000). The significant difference was observed between groups (Post Hoc test p = 0.000). They were KN-KK(-), KN-KP1, KN-KP2, KN-KP3, KK(-)-KP1, KK(-)-KP2, KK(-)-KP3, KP1-KP2, KP1-KP3, and KP2-KP3. Conclusions: Ethanol extract of Kenikir leaves can prevent the histological damage of the mice's kidney cell induced by Parasetamol. The increasing dose of the extract is followed by the increase of protective effect. Keywords: Kenikir leaves, histological damage, mice's kidney, Parasetamol
Analisis Molekuler Regio Core Promoter dan Precore/CoreIsolat Virus Hepatitis B 09IDSKAB-3 Ibnu Yudistiro; Afiono Agung Prasetyo; Yulia Sari
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: HBV replicates its DNA genome through reverse transcription from RNA intermediate. It is vulnerable to a high number of mutations during such reverse transcription which are frequently found in core promoter and precore/core regions. This study was aimed to identify genetic variation of HBV core promoter and precore/core regions of 09IDSKAB-3 isolate. Methods: DNA extraction was performed on 09IDSKAB-3 blood sample that was taken from Man Sex with Man Community. Core promoter and precore/core regions were determined by PCR using KL-28 and KL-6 primers and direct sequencing of the corresponding region. Molecular analysis was performed using MEGA 4.0. Results: Based on BLAST result, 09IDSKAB-3 HBV isolate had the highest similarity to isolate AP011085 from DKI Jakarta. Genetic variations A1726C in core promoter, and T1860C, C1877T, G1957C in precore/core region were found in 09IDSKAB-3 isolate. Conclusions: 09IDSKAB-3 HBV isolate was classified into genotype B and subgenotype B3 based on core promoter and precore/core region. The genetic variations found in this isolate may have influence to the replication efficiency and HBeAg/HBcAg production, therefore need further study. Keywords: hepatitis B virus, molecular analysis, core promoter region, precore/core region
In Vitro Antihelmintic Effect of Mahagoni (Swietenia mahagoni Jacq) Seeds Etanol ExtractAgainst Ascaris suum Goeze Yusuf Arif Salam; Yulia Sari; Sigit Setyawan
Nexus Biomedika Vol 6, No 2 (2017): Nexus Biomedika
Publisher : Nexus Biomedika

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Introduction: Mahagoni seeds contain flavonoid, saponin, and tannin that that have been known having anthelmintic effect. This study aimed to determine the antihelmintic effect of mahagoni (Swietenia mahagoni Jacq) seeds etanol extract against Ascaris suum Goeze in vitro. Methods:The study was a laboratory experimental research using post test only controlled group design. The subject were adult Ascaris suum Goeze worms. The sampling technique used was quota sampling. This research was using mahagoni (Swietenia mahagoni Jazq) seeds etanol extractwith concentration 30% w/v, 50% w/v, 70% w/v, 90% w/v, NaCl 0.9% (negative control), and albendazol 0.5% (positive control).Data were analyzed with Pearson correlation test to determine correlation between concentration of mahagoni (Swietenia mahagoni Jacq) seeds etanol extract and amount of worms death. Probit analysis has been done to find LC50. Results: Pearson correlation test showed significant result p=0.00 and strong correlation r = 0.87. Probit analysis results showed LC50mahagoni (Swietenia mahagoni Jacq) seeds etanol extract was 40.66% w/v. It means that 40,66% concentration of mahagoni (Swietenia mahagoni Jacq) seeds etanol extract can cause the death of half sample. Conclusions:Mahagoni(Swietenia mahagoni Jacq) seeds etanol extract had effects to the mortality of Ascaris suum Goeze in vitro with strong correlation between additional amount of extract concentration and amount of worms with LC50value= 40,66% w/v. Keywords :Antihelminthic, Swietenia mahagoni Jacq, alkaloid, Ascaris suum Goeze
Efek Nefroprotektor Ekstrak Biji Mahoni (Swietenia mahagoni (L.) Jacq.) terhadap Kerusakan Histologis Sel Ginjal Mencit (Mus musculus) yang Diinduksi Parasetamol Muvida Muvida; . Muthmainah; Yulia Sari
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Seed extract of Swietenia mahagoni (L.) Jacq. contains saponins, flavonoids, alkaloids, terpenoids, steroids, and tannins that may exhibit significant protection of kidney cells from free radicals. In present study, Swietenia mahagoni was evaluated for its nephroprotector effect on paracetamol-induced kidney cells histological damage in mice (Mus musculus). Methods: This research used experimental laboratory studies with post test only controlled group design. Samples were 28 male Swiss webster mice (2-3 months old) weighing + 20 g and divided into 4 groups, 7 mice each group. Sampling technique in this research was incidental sampling. The negative control group (KK (-)) and the positive control group (KK (+)) of mice were administered aquadest for 14 days. The first treatment group (KP1) and the second group treatment (KP2) of mice were administered mahagony seed extract with the dose of 11,2 mg/20 g and 22,4 mg/20 g body weight of mice for 14 days, respectively. Paracetamol was administered to groups of KK (+), KP1, dan KP2 on the 12th, 13th, and 14th day. On day-15th, mice were sacrificed and their kidneys were taken and stained with hematoxilin eosin (HE) for histological study. The kidney cells histological features were assessed based on quantifying of pyknosis, karyorrhexis, and karyolysis. Data were analyzed with the One-Way ANOVA test (? = 0.05) and Post Hoc Multiple Comparisons LSD test (? = 0.05). Results: The mean of kidney cells histological damage in mice for KK (-), KK (+), KP1 and KP2 were 9.57 + 0.701; 28.93 + 1.698; 9.28 + 0.873 and 27.79 + 1.651, respectively. Result of statistic analysis showed that there were significant differences of kidney cells damage score between KK (-) KK (+), KK (-) KP2, KK (+) KP1, KP1 KP2, and non significant differences between KK (-) KP1 and KK (+) KP2. Conclusions: Swietenia mahagoni (L.) Jacq. seed extract showed nephroprotector effect against paracetamol-induced kidney cells histological damage in mice. Keywords: mahagony seed extract, nephroprotector, kidney cells histological damage
Analisis Molekuler Regio Pre-S1, Pre-S2, dan S Isolat Virus Hepatitis B 09IDSKAB-3 Angga Dwi Prasetyo; Afiono Agung Prasetyo; Yulia Sari
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: The Hepatitis B Virus (HBV) pre-S1, pre-S2, and S region are essentially known for virus infection and replication. Eight genotypes (A to H) and nine subtypes (adw2, adw4, ayw1, ayw2, ayw3, ayw4, adrq+, adrq-, and ayr) of HBV have been identified worldwide. The aim of this study was to analyze HBV genetic variation in pre-S1, pre-S2, and S region, and to determine genotype and subtype of 09IDSKAB-3 HBV isolate from Men Who Have Sex With Men community in Surakarta. Methods: 09IDSAKAB-3 HBV DNA extraction was used as a template for amplication of pre-S1, pre-S2, and S region. The sequence results were then aligned by Clustal W with all of reference sequences reported in GenBank/DDBJ/EMNL. Genetic variation in pre-S1, pre-S2, and S region were identified using MEGA 4.0. HBV genotype and subgenotype were identified by phylogenetic analysis. HBV subtype was deduced on the basis of the predicted amino acid sequences of HBsAg. Results: Based on BLAST search in GenBank, 09IDSKAB-3 isolate was classified into genotype B3. D27E variation was found in pre-S1 region, and there were not genetic variation in pre-S2 and S region. Phylogenetic tree showed that 09IDSKAB-3 isolate was classified into genotype B3. Based on the basis of predicted amino acid sequences of HBsAg, 09IDSKAB-3 isolate was classified into subtype adw2. Conclusions: Overall, only D27E variation was found in this study. 09IDSKAB-3 isolate was classified into genoytpe B3 and subtype adw2. The amino acid variations found in the present report need further study. Keywords: HBV, pre-S1 region, pre-S2 region, S region
The Effect of Strawberry (Fragaria x ananassa) Fruit Juice on Renal Cell Histological Damage of Mice Induced by Rhodamine B Dea Saufika Najmi; Endang Listyaningsih Suparyanti; Yulia Sari
Nexus Biomedika Vol 4, No 1 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

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Background:Strawberry (Fragaria x ananassa) contains many antioxidant phytochemicals such as vitamin C, anthocyanins and ellagic acid that may exhibit significant protection of kidney cells from free radicals. In present study, Strawberry (Fragaria x ananassa) fruit juice was evaluated for its nephroprotector effect on mice renal damage induced by Rhodamine B. Methods:This research was an experimental laboratory study with the posttest only controlled group design. Samples were 28 male mice, Swiss webster type, 2-3 months old and 20 g of each weight. Samples were divided into 4 groups of 7 mice each. This research used consecutive sampling. Strawberry juice was given for 16 days in a row, while Rhodamine B (0.2 ml/20 gr of mice body weight) was given on 10th to 16th day. Negative Control Group (KK-) was given distilled water only. Positive Control Group (KK+) was given Rhodamine B. First Treatmennt Group (KP1) and Second Treatment Group (KP2) were given 0.4 ml and 0.8 ml of Strawberry fruit juice per 20 gr of mice body weight and Rhodamine B. On the 17th day, mice were sacrificed by neck dislocation. After that, the kidney was dissected out and made for preparation with paraffin block method and stained with Hematoxilin Eosin (HE). The score of renal cells damage was determined by pyknosis, karyorrhexis, and karyolysis. The data were analyzed with One-Way ANOVA test (? = 0.05) and Least Significant Differences (LSD) test (? = 0.05). Results:The result of One-Way ANOVA test showed that there was a significant difference in score of renal cells (p < 0.05). LSD test showed a significant difference in score of renal cells (p < 0.05) across all pair groups. Conclusions:Strawberry (Fragaria x ananassa) fruit juice showed nephroprotector effect against renal cell histological damage in mice induced by Rhodamine B. Keywords:Strawberry fruit juice, Rhodamine B, renal cell histological damage.
Pengaruh Pemberian Jus Stroberi (Fragaria x ananassa) Terhadap Kerusakan Histologis Sel Hepar Mencit yang Diinduksi Rhodamin B Risky Pratiwi; . Muthmainah; Yulia Sari
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Strawberry contains flavonoid, anthocyanin, ellagic acid and vitamin C that can protect the liver from free radicals. The aims of this research are to know the effect of strawberry juice and the increasing of its dose on the liver histological damage of mice caused by rhodamine B. Methods: This study was laboratory experimental research with the post-test only control group design. The laboratory animal used in this experiment was male Swiss webster mice with 2-3 months old and 20 grams of each weight. Twenty eight samples were divided into 4 groups (KKn, KP, KP1 and KP2), each group consisted of 7 mice. The sampling technique in this research was consecutive sampling. The normal control group (KKn) was given distilled water, while the exposure group (KP), the 1st treatment group (KP1), and 2nd treatment group (KP2) were given rhodamine B. The mice of KP1 and KP2 were given strawberry juice in dose 0.4 ml/20 gWt and 0.8 ml/20 gWt. These treatments were done for 16 days, then on the 17th day mice were killed with neck dislocation and their livers were made for preparation with paraffin block method and stained with HE. Liver cell histological damages were assessed based on quantifying necrotic cells of each 100 cells in the first zone and third zone. The data were analyzed using One-Way ANOVA test and Post Hoc LSD test (? = 0.05). Results: The data showed mean of necrotic cells in KKn, KP, KP1 and KP2 were 38.64 2.56; 157.64 2.87; 90.07 2.70; and 68.93 2.56, repeatedly. The results of One-Way ANOVA test showed p = 0.000. The results of LSD test showed significant difference between KKn-KP, KKn-KP1, KKn-KP2, KP-KP1, KP-KP2, and KP1-KP2 with p = 0.000 for each groups. Conclusion: Strawberry juice reduces mices liver cell histological damage caused by rhodamine B and the increasing dose of strawberry juice can reduce liver cell histological damage. Keywords: Strawberry juice, rhodamine B, liver cell histological damage
Pengaruh Pemberian Ekstrak Etanol Bit Merah (Beta vulgaris L.) Terhadap Kerusakan Histologis Sel Hepar Mencit (Mus musculus) yang Diinduksi Parasetamol Debora Marga Pangestika; . Suyatmi; Yulia Sari
Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Red beet (Beta vulgaris L.) has various components such as betalain, flavonoid, phenolic, ?-karoten, vitamin C and E, Fe, Zn, Cu, Mn, and Selenium. These components are potent to be antioxidant to protect liver from free radicals. The aims of this research were to evaluate hepatoprotective effect of ethanol extract of red beets on histologic damage of mices liver cells induced by paracetamol and the dose dependent of this extract. Methods: This was laboratory experimental research with the posttest only controlled group design. The subjects of this reaseach were 30 Swiss webster male mice (Mus musculus), 2-3 months old and 20 grams of weight, divided into 5 groups randomly. The groups were KK(-), KK(+), KP1, KP2, and KP3. Aquadest was administered to KK(-) and KK(+). Paracetamol was administered to KK(+), KP1, KP2, and KP3 on the day 12, 13 and 14. Red beet extracts were administered to KP1, KP2, and KP3 in dose 5.6 mg, 11.2 mg and 22.4 mg per 20 g BW of mice, successively. The hepatoprotective effects were assessed by counting the number of damage cells among 100 hepatocytes at central lobe zone of liver. Results: The result of One-Way ANOVA p = 0.000 (p < 0.05) showed a significant difference among each other group. The linear regression test p = 0.000 (p < 0.05) showed a correlation between increasing dosage and improving protective effect on mices liver cell damage. Conclusions: The ethanol extract of red beet had protective effect on histologic damage of mices liver cells induced by paracetamol. The increasing dosage of the red beet extract improved the protective effect to liver cells. Keywords : ethanol extract red beet, mice liver cells, paracetamol
Effectivity of NS1 Antigen Test for Early Detection of Dengue Fever in Surakarta and Surrounding Areas Arina Setyaningrum; Leli Saptawati; Yulia Sari
Nexus Biomedika Vol 4, No 1 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

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Background:.The first time of dengue virus infection can give symptoms as dengue fever. Dengue fever disease has symptoms that are not typical and may manifest as Dengue Hemorrhagic Fever and can progress to Dengue Shock Syndrome which can cause death if patients get treatment too late. Therefore, it is important to anticipate the delay in treatment so that requires a device to detection of dengue fever which is faster and more accurate. One is NS1 antigen detection methods. This study aimed to determine the effectiveness the NS1 antigen test for early detection of dengue fever in the region of Surakarta and surrounding areas. Method:.This study was a diagnostic test study using a community-based cross-sectional design. Sample size was 49 patients taken with consecutive techniques. Each samples performed detection of dengue NS1 antigen by Rapid strips test of immunochromatography and detection of genetic material of dengue virus by RT-PCR. Both of the data were tested with a diagnostic test. Result:.Diagnostic test results gave 67% of sensitivity, 89% of specificity, 67% of positive predictive value, 89% of negative predictive value, 6.09 of positive likelihood ratio, and 0.37 of negative likelihood ratio. The presence of a major change of the value of pretest probability (24%) to the value of the Post Test Probability (66%) indicated that the dengue NS1 antigen test device could provide diagnostic results which were important and useful. Conclusion:The NS1 antigen test is effective as diagnostic kit of Dengue virus infection due to its well specificity Keywords: Dengue Virus, Dengue NS1 antigen test, early detection of Dengue fever
Co-Authors . Muthmainah . Suyatmi Afiono Agung Prasetyo Alvian Oscar Irawan Amanda Yessica Anggara Ana Rima Setijadi Angga Dwi Prasetyo Annisa Avelia Arina Setyaningrum Asril &#039; Aticeh Aticeh Budi Afriyansyah Dahniar Endahfuri Dea Saufika Najmi Debora Marga Pangestika Dian Ariningrum Diana Hartaty Angraini Didik Gunawan Tamtomo Ekkim Al Kindi Emma Ayu Lirani Endang Listyaningsih Endang Listyaningsih S Endang Listyaningsih Suparyanti Fakhri Ras Gandari, Hijriyah Putri Helen Susanti Ibnu Yudistiro Ivan Setiawan Kamalia Kamarrudin Oemar Leli Saptawati Lilik Wijayanti Lina Juairiah Lina Kristanti Wibowo Makhdalena Makhdalena Mashuri, Yusuf Ari Muvida Muvida Novien Rialdy Nur Fitri Ayu Pertiwi Rina Fitriana Risky Pratiwi Samiaji Abbas Ras Sigit Setyawan Siti Marufah Sri Haryati Sugeng Purnomo Widia Susanti Yusuf Arif Salam