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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Program Kreativitas Mahasiswa - Penelitian Journal of Islamic Pharmacy Majalah Kesehatan FKUB Jurnal Penelitian dan Pengabdian Kepada Masyarakat UNSIQ Darmabakti : Junal Pengabdian dan Pemberdayaan Masyarakat Caring : Jurnal Pengabdian Masyarakat Tri Dharma Mandiri: Diseminasi dan Hilirisasi Riset Kepada Masyarakat (Jurnal Pengabdian Kepada Masyarakat) Jurnal Bioteknologi & Biosains Indonesia
VALENTINA YURINA
Program Studi Farmasi, Fakultas Kedokteran Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Prolonged-heated High-Fat Diet Increase the Serum LDL Cholesterol Level and Induce the Early Atherosclerotic Plaque Development in Wistar Rats Yurina, Valentina; Yunita, Ema Pristi; Raras, Tri Yudani Mardining; Rudijanto, Achmad; Handono, Kusworini
Journal of Tropical Life Science Vol 9, No 1 (2019)
Publisher : Journal of Tropical Life Science

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Abstract

Rats are one of the most widely used animal models in health research. However, since rats are relatively resistant to atherosclerosis, the transgenic rats often used to study atherosclerosis in rats. Our study suggests a prolonged-heated lipid to induce atherosclerosis in rats, therefore provide a more low-cost option to study atherosclerosis in rats. Aims of this study is to compare the effect of heated high-fat diet (HFD) to the LDL and HDL cholesterol level and foam cell formation in the Wistar rat animal model. Rats were divided into three groups, control group was given with the normal diet, and the other two treated groups received oxidized HFD (heated HFD) and HFD, respectively. The heated HFD contain lard that was previously heated at 190°C for 24 h. Diet was given for 8 weeks. The serum LDL and HDL cholesterol level were measured before and 4th and 8th weeks after treatment with help of colorimetric measurement. The aorta analyzed for the foam cell formation after HE staining using the light microscope.  The prolonged- heated HFD significantly increased the LDL cholesterol from the 4th week of the treatment (p = 0.023). The rats aortic from HFD and heated HFD treated groups showed a mononuclear cells infiltration and the early foam cell formation. The heating process of the lipid caused lipid oxidation which significantly increased the LDL cholesterol level of the animal model and foam cells formation. This study suggests that Wistar rats with heated HFD could demonstrated early atherosclerosis plaque development.
Design, Construction and Expression of Spike Highly Conserved Region (HCR) SARS-CoV-2 and Cholera Toxin Subunit B Fusion Protein in Lactococcus lactis NZ3900: Construction of recombinant plasmid in Lactococcus lactis Kesuma, Suryanata; Adianingsih, Oktavia Rahayu; Winarsih, Sri; Widodo, Nashi; Yurina, Valentina
Journal of Tropical Life Science Vol. 15 No. 2 (2025): In Press
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/2xf1qp96

Abstract

Prevention of SARS-CoV-2 transmission has primarily been achieved through vaccination, which is generally administered via injection and may cause discomfort. No commercially available SARS-CoV-2 vaccines can be administered via the mucosal route. However, recent advancements have demonstrated that vaccination with Lactococcus lactis enables vaccine delivery through the mucosa. A promising target for SARS-CoV-2 vaccine materials is the highly conserved region (HCR) of the SARS-CoV-2 spike (SARS-CoV-2 HCR Spike). Vaccine efficacy is enhanced by adding Cholera Toxin Subunit B (CTB) as an adjuvant. HCR and CTB proteins were recombinantly fused using a synthetic gene with optimized codons. This study aimed to construct a fusion protein of the SARS-CoV-2 spike protein and CTB in L. lactis strain NZ3900. The construction and expression of fusion proteins were analyzed using sequencing and protein electrophoresis. Codon optimization resulted in a Codon Adaptation Index value of 0.93 and a GC content of 27.06%. The cloning results revealed the formation of L. lactis colonies expressing the Fusion protein of the SARS-CoV-2 HCR Spike and CTB, which formed yellow colonies on the selection Elicker medium. PCR and sequencing confirmed the presence of the hcr-ctb gene, with a length of 981 bp and 100% sequence similarity. The Fusion protein of the SARS-CoV-2 HCR Spike and CTB was successfully expressed with a molecular weight of >35 kDa. In conclusion, we successfully constructed a Fusion protein of the SARS-CoV-2 HCR Spike and CTB in L. lactis NZ3900 as a potential vaccine candidate for oral administration to prevent SARS-CoV-2 infection.
Co-Authors Achmad Rudijanto Alvan Febrian Shalas Anggita Rosiana Putri Anggita Rosiana Putri Angi Nurkhairina, Angi Anita Puspa Widiyana Ardina Pramesti Putri, Ardina Pramesti Bachtiar Rifai Pratita Ihsan Bachtiar Rifai Pratita Ihsan Diah Royani Meisani Ema Pristi Yunita, Ema Pristi Eva Putri Arfiani Fredo Tamara, Fredo Ihsan, Bachtiar Rifai Pratita Kesuma, Suryanata Kusworini Handono Luthfi Ahmad Muchlashi Megasari, Suci Muchlashi, Luthfi Ahmad Nabila Ayu Safitri, Nabila Ayu Nashi Widodo Oktavia Eka Puspita Oktavia Eka Puspita, Oktavia Eka Oktavia Rahayu A, Oktavia Rahayu Oktavia Rahayu Adianingsih Poejiani, Soeyati Sri Winarsih Tamara Gusti Ebtavanny Thoha Muhajir Albaar, Thoha Muhajir Tri Yudani Mardining Raras