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All Journal Agroland : Jurnal Ilmu-Ilmu Pertanian AGROTEKBIS AGROLAND: The Agricultural Sciences Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Unram Journal of Community Service (UJCS) AGROTEKBIS: Jurnal Ilmu Pertanian AGROLAND: The Agricultural Sciences Journal Mitra Sains
Ramal Yusuf
Program Studi Agroteknologi Fakultas Pertanian Universitas Tadulako, Palu
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Physics Social Sciences

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THE GROWTH OF JACKFRUIT (ARTOCARPUS HETEROPHYLLUS L.) SHOOTS ON VARIOUS CONCENTRATION BENZYLAMINO PURINE (BAP) IN VITRO Frisca Nanda Bulo; Enny Adelina; Ramal Yusuf; Hawalina Kasim
AGROLAND: The Agricultural Sciences Journal Vol 5, No 2 (2018)
Publisher : Tadulako University

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Abstract

The development of Central Sulawesi superior commodity,which is Tulo-5, the drough-resistant cultivars, through tissue culture technique can be used as an alternative, in addition to conventional breeding. This study aims to determine the growth of jackfruit shoots in  various concentrations of benzylamino purine in vitro. The research has been conducted at the Laboratory of Plant Biotechnology, Agriculture Faculty of Tadulako University, Palu.  The research began on December 2017 and ended up on February 2018. This research used Completely Randomised Design of one-factor treatment which is BAP concentrations consist of 4 stage which is1,5 ppm BAP, 2,0 ppm BAP, 2,5 ppm BAP, 3,0 ppm BAP. The result showed that 6 weeks after planting, BAP concentration had significant effect on shoots time to emerge and shoots number but no significant effect on leaf number.  The result of Tukey’s HSD test showed that concentration2,5 ppm gave the fastest shoot emergence with an average of 2,875 days after planting, in concentration 2,0 ppm gave the highest number of shoots with an average of 2.125 shoots per explant, and for the highest number of leaves found in the concentration of 2,0 ppm with the average number of leaves is 1 strand per explant. 
EFFECT OF TWO SEAWEED PRODUCTS ON RADISH (RAPHANUS SATIVUS) GROWTH UNDER GREENHOUSE CONDITIONS Ramal Yusuf; Paul Kristiansen; Nigel Warwick
AGROLAND: The Agricultural Sciences Journal Vol 2, No 1 (2015)
Publisher : Tadulako University

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Abstract

Several studies have revealed a wide range of beneficial effects of seaweed products applications on plants growth and development. Seaweeds may cause different effect in plant responses because there are a range possible factors acting in concert. These factors may include to the amount of plant growth regulator (PGR) and nutrients present in the products. PGR activity in two seaweed products (Maxicrop® and Seasol®) was investigated using Radish (Raphanus sativus). Treatments included a control, standard concentrations of the seaweed Products (SS1 and MC1),    2 and 4 times the recommended rates (SS2, SS4, MC2 and MC4) ashed seaweed products (SS-ash and MC-ash), and equivalent amounts of N, P and K to that found in seaweed products (SS-NPK and MC-NPK). The results obtained show that both Maxicrop® and Seasol® can significantly increase crop performance. Maxicrop® increased chlorophyll content and root and shoot growth, while Seasol® only increased the chlorophyll content.   Key Words: Plant growth regulator, radish, seaweed.
PROPAGATION OF LEMBAH PALU SHALLOT SOMATIC EMBRYOSAS EFFORTSTO PROVIDE GOOD QUALITY SEED Maemunah,Ramal Yusuf,Hawalina, Yusran
AGROLAND: The Agricultural Sciences Journal Vol 2, No 2 (2015)
Publisher : Tadulako University

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Abstract

Shallot (Allium wakegi Arakic.v.LembahPalu) is a horticulture commodity prospective as a very good business opportunity, since becomesthe major ingredient of fried shallot, the most unique souvenir of Palu. The productivityhowever, is generally low due to lack in availability of good seed and seed production technology.The purpose of this study is to develop seed production technology through multiplication of somatic embryos. This assessment is the second part of research stages regarding the development of shallotsomatic embryo technology in Central Sulawesi. The first stage of the research is setrilization and callus induction of Lembah Palu shallot. This second stage is maturation dan germination of somatic embryos.The experiment was arranged as factorial completely randomized design, comprising of two factors. The first factor was 2,4-D concentration; consisting of three levels i.e 0.00, 0.25 and 0.50 mg/L. The second factor is kinetin concentration with the same levels as in 2,4-D. Each treatment combination was replicated three times, thus there were 27 experimental units. The result showed that addition of kinetin in MS basal media strongly affected the percentage of mature embryos and the percentage of mature embyos producing shoots, with the highest rate was obtained from the media fortified with 0,50 mg/L kinetin (26,913% and 43,318% respectively). Addition of 2,4-D to the MS basal media tended to inhibit the rate of regenerating shoot from the embryos. There is no interaction between 2,4-D and kinetin concentrations in affecting maturation and germination of the somatic embryos.
WASTE APPLICATION OF SEAWEED (EUCHEUMA COTTONII) ON PLANT GROWTH AND RESULTS OF MUSTARD (BRASSICA JUNCEA L.) suriyani suriyani; Ramal Yusuf; Abd Syakur
AGROLAND: The Agricultural Sciences Journal Vol 4, No 2 (2017)
Publisher : Tadulako University

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Abstract

 Seeing the importance of consuming vegetables and increasing of  public demand each day, to increase the maximum production using the fertilizer is one of the solutions, either organic fertilizers or inorganic fertilizers. The objective of this study is to determine the effect of seaweed Eucheuma cottonii waste application on growth and yield of mustard. This study was conducted on  January and February 2016. Located in Sidondo, district torch and Seed Technology Laboratory Sciences, Tadulako University, Palu, Central Sulawesi. This study was conducted using a randomized block design (RBD), with treatment consisting of R0 (control), R1 (20% of waste seaweed), R2 (40% of waste seaweed), R3 (60% of waste seaweed), R4 (80% of waste seaweed) and R5 (100% of waste seaweed). Each treatment was repeated four times so that there are 24 experimental units. The results showed that the treatment of waste RO seaweed significantly affect the growth and yield of mustard. R5 (100% of waste seaweed) generating plant height, leaf number, fresh weight, dry weight, better than other treatments.
APPLICATION OF SOME TYPES OF SEAWEEDS ON THE GROWTH AND YIELD OF SHALLOT (Allium ascalonicum L.) Ramal Yusuf; Abdul Syakur; Budiatno Budiatno; Hidayati Mas'ud
AGROLAND: The Agricultural Sciences Journal Vol 3, No 2 (2016)
Publisher : Tadulako University

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Abstract

Shallot plant is often used as a flavor enhancer and appetite enhancer food. Research on shallotcrop has been done. Application of seaweedsgive a different effect on increasing the productivity and growth of shallots. This study aims to determine the effect of applying different types of seaweed on growth and yield of shallot variety Lembah Palu. This research was conducted at the Green House of Faculty of Agriculture, University of Tadulako from March to May 2016. The study conducted using a randomized block design (RBD).  The treatments: B0 = control, B1 = NPK 0.2 g / pot, B2 = (Cauelerpa sp) 100 ml/pot, B3 = (Sargassum sp) 100 ml/pot, B4 = (Eucheuma cottonii) 100 ml /pot, B5 = Sea lettuce (Ulva sp) 100 ml/pot. there are six treatments, each treatment was replicated four times, so there are 24 experimental units. The results indicatedthat the application of various types of seaweed significant effect on plant height, fresh weight of the plants, the fresh weight of tuber and diameter of the bulb.
CALLUS INDUCTION OF CACAO CLONE SULAWESI 1 ON VARIOUS CONCENTRATIONS OF 2,4 -D AND COCONUT WATER VIA IN VITRO CULTURE Asmila Asmila; Zainuddin Basri; Ramal Yusuf; Hawalina Hawalina
AGROLAND: The Agricultural Sciences Journal Vol 4, No 1 (2017)
Publisher : Tadulako University

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Abstract

Cacao is one of important plantation crops grouped in the Sterculiaceae family.  Sulawesi is the main area of cacao production and has a number of superior clones, such as Sulawesi 1 and Sulawesi 2.  Based on data in 2012/2014 cacao production to consumption reached 174,000 tons, while in 2013/2014 was projected a deficit of 115,000 tonnes. Nonetheless, cacao agribusiness in Indonesia is still facing complex problems, among others gardener productivity is still low due to borer attacks cacao, the quality of products and the number is still low and still not optimal development of cacao products and providing superior amount of cacao seedlings. The primary problem of cacao production recently is low productivity.  The main cause of low cacao productivity in Central Sulawesi is the use of inferior clones.  To enhance cacao productivity, it is crucial to use cacao clones having high genetic potential via tissue culture or micropropagation techniques.  The aim of this experiment was to assess the effect of different concentrations of 2,4-D and coconut water on the growth of cacao callus via in vitro culture.  This experiment used Completely Randomozed Block Design in factorial patteren with treatments tested namely 2,4-D and coconut water concentrations.  The concentrations of 2,4-D tested including 1 ppm, 2 ppm and 3 ppm, whilst coconut water concentrations tested consisting of 10%, 15% and 20%, and therefore there were 3 x 3 = 9 treatment combinations.  Each treatment utilized 4 replications; and each unit combination used 5 explants (staminodia).  Results of this experiment indicated that the addition of 3 ppm 2,4-D and 10% coconut water had a highly significant effect on the callus color 4 weeks after culture.  The addition of 3 ppm 2,4-D in culture media showed a significant effect on callus color 4 weeks after culture, but had an insignificant effect on the callus formation, callus color 8 weeks after culture an callus texture.  Supplementation of 20% coconut water had a significant effect on callus texture 8 weeks after culture, whilst the addition of 10% coconut water showed a significant effect on callus color 4 weeks after culture.
BIOASSAY DEVELOPMENT USING PEA (Pisum sativum L.) Ramal Yusuf; Paul Kristiansen; Nigel Warwick
AGROLAND: The Agricultural Sciences Journal Vol 1, No 1 (2014)
Publisher : Tadulako University

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Abstract

The term bioassay is used to describe the use of living material to test the effect of known and putative biologically active substances. In places where advanced analytical equipment is not available, rapid screening using simple, affordable methods is needed. Seeds of Seeds of peas were germinated (Arditti and Dunn, 1969) and grown in the dark at 20°C for 2 days. Five pre-germinated seeds immersed in 30 mL solution containing test solutions of auxins (IBA), cytokinins (kinetin) and a gibberellin (GA3) all seeds were distributed at randomly to Petri dishes containing the test solution. Auxin activity peak was equivalent to 0.005 µM, while cytokinin activity peaked at 0.5 µM, and gibberellin at 0.5 to 5 µM based on root measurement.   KeyWords : Bioassay, elongation, Pea.
PERTUMBUHAN TUNAS ANGGUR HITAM (Vitis vinifera L.) PADA BERBAGAI KONSENTRASI BENZYLAMINO PURIN DAN INDOLEBUTYRIC ACID Mardiyah Mardiyah; Zainuddin Basri; Ramal Yusuf; Hawalina Hawalina
Agroland: Jurnal Ilmu-ilmu Pertanian Vol 24, No 3 (2017)
Publisher : Universitas Tadulako

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Abstract

This study aimed to determine media compositions supplied with BAP and IBA suitable for the growth of black grape shoots. This research was conducted at the Laboratory of Plant Biotechnology, Faculty of Agriculture, University of Tadulako, during April to September 2016. The study used a factorial completely randomized design. Two factors were tested, the concentrations of BAP i.e. 1.50 ppm, 2.00 ppm and 2.50 ppm; and the concentration of IBA i.e. 0.00 ppm, 0.25 ppm and 0.50 ppm. There were nine treatment combinations and each combination treatment was replicated three times to obtain 27 experimental units. Each experimental unit using an explant. Data were analyzed using analysis of variance and followed by Honestly Significant Difference test at level of 5% if the treatment effects were significant. The results showed that the composition of the culture media supplied with 2.00 ppm BAP without IBA is able to stimulate the formation of black grape leaves at the earliest, at an average of 18.67 days per explant, while the media added with 2.00 ppm 0.25 ppm BAP together with IBA can lead to longest shoot growth, at an average of 2.13 cm per bud. The culture media applied with 2.50 ppm BAP can trigger earliest shoot growth and highest number of leaf formation which were 11.11 days after culture and 2.44 leaves per explant. Earliest formation of black grape shoots of 11.89 days after culture is stimulated by the addition of 0.25 ppm IBA.
TEMPERATURE AFFECTING THE FLOWERING OF STURT’S DESERT PEA (Swainsona Formosa) Ramal Yusuf
Agroland: Jurnal Ilmu-ilmu Pertanian Vol 15, No 2 (2008)
Publisher : Universitas Tadulako

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Abstract

Sturt;s Desert Pea (SDP) adalah suatu tanaman legum. Tanaman ini berpotensi sebagai tanaman bunga dalam pot, juga cocok pada pot-pot gantung dan bunga potong. Keberhasilan secara komersial sangat tergantung pada produksi yang konsisten dari tanaman yang berkualitas.  Tujuan penelitian ini adalah menemukan kondisi lingkungan yang sesuai untuk produksi tanaman. Faktor lingkungan yang diteliti adalah temperatur. Untuk mengetahui pengaruh temperatur pada pembungaan, maka penelitian  dilakukan  dengan  mengatur  temperatur  pada  25°C  konstan,  temperatur  10°C  malam  dan  22°C  siang,  dan  perlakuan  ketiga tanaman  ditumbuhkan  pada  temperatur  18°C  malam  dan  30°C  siang.  Hasil  penelitian  menunjukkan  bahwa  tanaman  yang ditumbuhkan pada temperatur 18°C malam dan 30°C siang menghasilkan bunga lebih banyak dari  perlakuan temperatur lainnya.
PERTUMBUHAN DAN HASIL TANAMAN SAWI (Brassica juncea L.) YANG TUMBUH PADA BERBAGAI MEDIA TANAM DAN PEMBERIAN PUPUK ORGANIK CAIR Sitti Patima; Sakka Samudin; Ramal Yusuf
Agroland: Jurnal Ilmu-ilmu Pertanian Vol 21, No 2 (2014)
Publisher : Universitas Tadulako

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Abstract

The experiment aimed to study the effect of media and to see the effect ofliquid organic fertilizer taking from goat manure on growth yield of mustard. This research has been conducted in the green house Faculty of Agriculture, University of Tadulako Palu from March 2014-April 2014. The research using Randomized Block Design (RBD), two factors. The first factor is the use of various types of media that is soil (M1); soil+sawdust (M2); soil+rice husk (M3). The second factor a liquid organic fertilizer of goat manure consisting of 3 levels ie1.6% liquid organic fertilizer/plant (C1), giving 2.4% /plants (C2) and the provision of 3.2% /plants (C3). Thus there are 9 treatment combination srepeated 3 times, so there are 27 experimental units. The results showed the use of various types of media and liquid organic fertilizer in fluence on components of plant height, leaf number, leaf area, fresh weight of root, leaf fresh weight, root dry weight, and dry weight of the plant leaf.  Based on the results of the study showed that aplication of 3.2% liquid organic fertilizer/plant (C3) in the mustard plant significant effectonall components of the observation. Medium soil+rice husk (M3) effect on growth of mustard. The interaction between the concentration of liquid organic fertilizers and media significant effecton the components of plant height, leaf area, fresh weight of roots, leaf fresh weight, root dry weight, dry weight of the leaf. Key Words : Mustard Plants, media, liquid organic fertilizer.
Co-Authors Abd Syakur Abd. Syakur Abd. Syakur Abd. Syakur Abdul Hadid Abdul Syakur Abdul Syakur Abdul Syakur Adam Malik Aiyen Tjoa Al Riski, Mohamad Alimudin Laapo Aminuyati An Yakhin Putos Arifuddin Lamusa Asmila Asmila Asmila Asmila Budiatno Budiatno Budiatno Budiatno Darwis Darwis Dastar Sarro Enny Adelina Esna Esna Fadel Fadel Fadel, Fadel Fajrin Fajrin Frisca Nanda Bulo Frisca Nanda Bulo Golar Golar Hadayani Hadayani Hadayani Hadayani Hasriani Muis Hawalina Hawalina Hawalina Hawalina Hawalina Hawalina Hawalina Hawalina Hawalina Kasim Hawalina Kasim, Hawalina Henry N .Barus Hidayati Mas'ud Hidayati Mas’ud Hijra Juanda Imran Rachman Intan yana sakti Jeki Jeki Maemunah Maemunah Maemunah Maemunah Mahfudz Mahfudz Mardiyah Mardiyah Mohammad Ansar Mohammad Rifqi Arianto Muhammad Anshar Muhammad Anshar Pasigai Mustakim Mustakim Naharuddin Naharuddin Ni luh Dian Purnama Sari Ni Made Setianingsih Ni Wayan Suastini Nigel Warwick Niluh Dewi Sri Susanti Nuraeni Nuraeni Oktaviana, Farnita Paul Kristiansen Putra, Bayu Wahyu Ramli Ratu Mentari Dewi Ratu Mentari Dewi Renaldi Renaldi S, Wandaria Sakka Samudin Samsurizal, M. Sulaeman Silvani Silvani Silvani, Silvani Sitti Patima Sri Anjar Lasmini Sri Anjar Lasmini Sudirman Daeng Massiri Suriyani Suriyani Syamsuddin Laude Trisnawati Trisnawati Vikram Tansidi Yohanis Tambing Yolanda Eva Rosalina Yusran Yusran Zaenuddin Zaenuddin, Zaenuddin Zainuddin Basri Zainuddin Basri