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Segregation Analysis of Transgenic Rice Plants cv Rojolele Harboring cry1B Gene and Plant Selection for Potential to Yellow Stem Borer Estiati, Amy; Sulistyowati, Yuli; Zahra, Fatimah; Nurhasanah, Ade Nena; Rachmawati, Syamsidah; Loedin, Inez Hortenza Slamet
Annales Bogorienses Vol. 16 No. 1 (2012): Annales Bogorienses
Publisher : BRIN

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Abstract

Transgenic rice plants harboring resistant gene to yellow stem borer, cry1B gene had been obtained. However, the cry1B gene in second generation of transgenic rice lines still segregating following Mendelian ratio 3:1. For further use in the breeding programs, it is important to ensure that the gene is dominant gene and the plants are homozygous for cry1B gene. Selection of homozygous transgenic rice lines containing cry1B gene at third, fourth and fifth generation had been conducted by PCR. The presence of cry1B gene was determined by showing the PCR product of 1.9 kb. Segregation analysis proved that six transgenic rice lines i.e. 3R7.8.15.1, 3R7.8.15.9, 3R7.8.15.10, 3R7.8.15.15, 3R7.8.15.21, 3R7.8.15.29 are homozygous lines for cry1B gene. Moreover, bioassay studies at vegetative stage on six homozygous transgenic rice lines showed that these transgenic rice lines are potential resistant to yellow stem borer comparing with non-transgenic plants, with the score of 0 (indicated no symptom) for six transgenic lines and score of 9 (more than 60% damaged tillers ) for non-transgenic plants. However, to confirm the efficacy of cry1B gene to yellow stem borer in natural condition, confined field trial in endemic area of yellow stem borer should be conducted. 
Self-Control and Hedonism: How Generation Z Paylater Users in Bandar Lampung Manage Their Finances Defanko, Velicia Anindita; Nurhayaty, Any; Zahra, Fatimah
Philanthropy: Journal of Psychology Vol. 9 No. 2 (2025): (July-December)
Publisher : Faculty of Psychology, Universitas Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26623/philanthropy.v9i2.12782

Abstract

Generation Z is known as the group most familiar with technology, making them more susceptible to using paylater services. Self-control and hedonistic tendencies are assumed to play a role in influencing their financial management behavior. This study aims to examine the relationship between self-control, hedonism, and financial management among paylater users in Bandar Lampung. The research design used was quantitative correlational with 332 participants aged 17–28 years selected through purposive sampling. The results of multiple regression analysis showed that self-control and hedonism had a significant effect on financial management with a significance level of 0.01 < 0.05. Partially, self-control has a strong positive influence (p=0.001) and an effective contribution of 14.9%, while hedonism shows a significant negative influence (p=0.004) and an effective contribution of 5.5%. These findings confirm that self-control is more dominant in shaping healthy financial management behavior than hedonism, which tends to have the opposite effect.
The relationship between workplace spirituality and organizational citizenship behavior among health workers in christian-based hospitals: A quantitative correlational study Chindie Mutiara Dihan; Tansri Adzlan Syah; Fatimah Zahra
Empathy : Jurnal Fakultas Psikologi Vol. 8 No. 2 (2025): [Edition December] EMPATHY Journal Faculty of Psychology
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/empathy.v8i2.31431

Abstract

This study aims to analyze the relationship between workplace spirituality and organizational citizenship behavior (OCB) among healthcare professionals in Christian faith-based hospitals. A quantitative research design was employed using a total sampling technique involving 113 full-time employees. The workplace spirituality scale was developed based on Milliman’s conceptualization, encompassing three dimensions meaningful work, sense of community, and alignment with organizational values (α = 0.956). The OCB scale was constructed following Organ’s framework, consisting of five dimensions: altruism, conscientiousness, sportsmanship, courtesy, and civic virtue (α = 0.940). Pearson correlation analysis revealed a very strong and significant relationship between workplace spirituality and OCB (r = 0.961; p < 0.001). These findings indicate that workplace spirituality plays a critical role in enhancing organizational citizenship behavior among healthcare professionals.
Komunikasi Persuasif Pimpinan Terhadap Peningkatan Prestasi Mahasiswa Fatimah Zahra; Riswanto
Jurnal Khabar: Komunikasi dan Penyiaran Islam Vol. 7 No. 2 (2025): Desember
Publisher : STAI Bumi Silampari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37092/khabar.v7i2.1227

Abstract

This study aims to examine the influence of persuasive leadership communication on improving student achievement in higher education. Based on the hypothesis regarding the effect of persuasive leadership communication on student achievement, the F-test results indicate that the regression model is statistically significant simultaneously, with an F value of 49.901 and a significance level of 0.000 (p < 0.05). Partially, the t-test results show that openness (t = 2.516; p = 0.013), support (t = 2.908; p = 0.004), positive attitude (t = 3.926; p = 0.000), and equality (t = 2.254; p = 0.026) have a significant influence on student achievement. In contrast, empathy does not have a statistically significant effect (t = 1.690; p = 0.093). The most dominant dimension is a positive attitude, with the highest beta coefficient (? = 0.259).This research employed a quantitative method involving 150 students from the Faculty of Social and Political Sciences at Universitas Muhammadiyah Bengkulu, using a Likert-scale-based questionnaire. Data analysis was carried out using descriptive statistics, Pearson correlation, and multiple linear regression via SPSS. The findings highlight the importance of leadership communication strategies that are open, supportive, positive, and egalitarian in building an academic environment that fosters and motivates student achievement. This study contributes both theoretically and practically to the development of leadership communication in higher education institutions.
Pengaruh Model Problem Based Learning (PBL) terhadap Hasil Belajar Peserta Didik Kelas V SD pada Materi Keliling Bangun Datar Zahra, Fatimah; Masniladevi, Masniladevi; Andika, Refiona; Ningsih, Yarisda
TSAQOFAH Vol 5 No 4 (2025): JULI
Publisher : Lembaga Yasin AlSys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/tsaqofah.v5i4.6605

Abstract

The low academic performance of students in mathematics serves as the primary motivation for this study, indicating that conventional teaching methods have not yet succeeded in fostering effective learning processes. The aim of this research is to analyze the effect of the Problem-Based Learning (PBL) model on students' learning outcomes in the topic of perimeter of plane figures. A quantitative approach was employed using a one-group pretest–posttest experimental design. The sample consisted of fifth-grade students (class VB) selected through cluster sampling as part of probability sampling. The results show an improvement in learning outcomes following the implementation of the PBL model, as evidenced by an increase in average scores from 29.86 (pretest) to 72.71 (posttest). A statistical test using the Mann-Whitney method yielded a significance value of 0.002 (p < 0.05), indicating a significant effect of the PBL model on student learning outcomes. It can therefore be concluded that the application of the PBL model contributes positively to enhancing students' conceptual understanding of mathematics, particularly in the topic of perimeter of plane figures, and can be implemented regularly to foster more meaningful learning experiences.
Aktivitas Antioksidan Ekstrak dan Antihiperpigmentasi Serum Biji Alpukat (Persea americana Mill.) sebagai Inhibitor Enzim Tirosinase Fatimah Zahra; Arini Khaerunnisa; Dhyneu Dwi Jayantie; Eva Vauziah; Irfan Iryana
Journal of Pharmascience Vol. 12 No. 2 (2025): Jurnal Pharmascience
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v12i2.21458

Abstract

Melanin merupakan pigmen utama yang memiliki fungsi melindungi kulit dari kerusakan akibat paparan sinar ultraviolet. Sintesis melanin berlebih dapat menyebabkan terjadinya hiperpigmentasi. Kandungan utama dalam biji alpukat (Persea americana Mill) adalah katekin yang berpotensi sebagai inhibitor enzim tirosinase. Tujuan dalam penelitian ini adalah untuk membuat formulasi sediaan serum ekstrak biji P.americana Mill dan mengetahui bagaimana aktivitas penghambat enzim tirosinase. Sampel Biji P.americana di maserasi dengan etanol 70%. Diformulasi dengan tiga formula yaitu F0 sebagai basis, F1 formula dengan 50x nilai antioksidan, dan F2 formula dengan 100x nilai antioksidan. Uji stabilitas sediaan dilakukan selama 28 hari uji stabilitas sediaan meliputi uji organoleptis, viskositas, pH, daya sebar, dan homogenitas. Hasil penelitian menunjukkan %rendemen ekstrak sebesar 13,168%. Hasil skrining fitokimia ekstrak Biji P. americana mengandung flavonoid, tanin, saponin, steroid dan alkaloid. Ekstrak biji P. americana memiliki nilai IC50 sebesar 22,26 ppm. Pengujian stabilitas sediaan serum ekstrak biji P. americana memiliki stabilitas yang baik. Aktivitas inhibisi enzim tirosinase F0, F1, F2 dan serum komersial memiliki nilai IC50 masing-masing sebesar 3274, 3585, 2809 dan 2165 ppm. Aktivitas inhibisi enzim tirosinase sediaan serum ekstrak biji P. americana mempunyai nilai IC50 yang paling baik berada pada sediaan serum dengan formula 100x nilai antioksidan. Kata Kunci: Alpukat, Antioksidan, Hiperpigmentasi, Serum, Tirosinase  Melanin is the main pigment that has the main function of protecting the skin from damage due to ultraviolet exposure, excess melanin synthesis can lead to the occurrence of hyperpigmentation. The main ingredients in Avocado Seeds (Persea americana Mill) are catechins which have potential as tyrosinase enzyme inhibitors. The aim in this study was to create a formulation of P. americana Mill seed extract serum preparation and to find out how tyrosinase enzyme inhibitor activity in P. americana seed extract serum preparations. A sample of P. americana seed was macerated with 70% ethanol. It is then formulated with three formulas namely F0 as the base, F1 formula with 50x the antioxidant value, and F2 formula with 100x the antioxidant value. A preparation stability test was performed over a 28-day preparation stability test covering organoleptic, viscosity, pH, dispersibility, and homogeneity assays. The tyrosinase enzyme inhibition assay was analyzed in vitro. Results of phytochemical screening of P. americana seed extract contain flavonoid group compounds, tannins, saponins, steroids and alkaloids. P. americana Seed Extract has an IC50 value of 22.26 ppm. Stability testing of serum preparation P. americana Seed extract has good stability. The inhibitory activity of tyrosinase enzyme F0, F1, F2 and commercial serum had IC50 values of 3274, 3585, 2809 and 2165 ppm respectively. The inhibitory activity of tyrosinase enzyme of serum preparation of P. americana seed extract had the best IC50 value in serum preparation with formula 100x antioxidant value.