Claim Missing Document
Check
Articles

Found 21 Documents
Search

Sumber Glukomanan Dari Edible Araceae Di Jawa Timur Gustini Ekowati; Bagyo Yanuwiadi; Rodiyati Azrianingsih
Indonesian Journal of Environment and Sustainable Development Vol 6, No 1 (2015)
Publisher : Graduate Program

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Sumber glukomanan dari edible Araceae terdapat pada genus Amorphophallus, Colocasia, Xanthosoma, Alocasia. Eksplorasi mengenai famili Araceae  mencakup berbagai macam tumbuhan Monokotil dengan ciri khas bunga majemuk bertipe "tongkol" yang berseludang (spatha). Araceae merupakan salah satu famili tanaman yang bermanfaat sebagai sumber makanan karena memiliki umbi yang mengandung karbohidrat, protein, glukomanan.  Tujuan pelaksanaan penelitian ini adalah untuk mendiskripsikan jenis dari famili Araceae di Jawa Timur yang berpotensi sebagai sumber glukomanan dan mengukur kadar glukomanannya. Pengambilan sampel Araceae di wilayah Jawa Timur dikategorikan menjadi 4 (empat) area geografis, yaitu: a. wilayah selatan dan tengah Jawa Timur (Blitar, Malang, Lumajang); b. wilayah timur Jawa Timur (Banyuwangi, Situbondo, Bondowoso); c. wilayah barat Jawa Timur (Madiun, Nganjuk, Tuban); d. wilayah pulau Madura (Bangkalan, Sampang, Pamekasan).  Pada tiap studi area diambil minimal tiga tanaman untuk masing-masing sampel spesies dari famili Araceae  secara acak, untuk diamati dan dideskripsikan keseluruhan bagian tanaman tersebut. Pengamatan morfologi dilakukan visual  maupun dengan alat bantu kaca pembesar dan mikroskop. Kadar glukomanan dianalisis setelah sentrifugasi ektrak umbi. Hasil pengamatan/pengukuran itu disusun dalam suatu tabel determinasi. Dari kunci tabel ini akan terlihat adanya perbedaan morfologi dan kadar glukomanan dari anggota famili Araceae yang ditemukan.  Diperoleh 12 jenis dari empat genus Araceae di 4 area geografis di Jawa Timur yang mengandung glukomanan.  Kadar glukomanan  sebagai berikut: tertinggi  Amorphophallus muelleri Bl. (porang) 9,92 % (berat basah), A.paeoniifolius (suweg) 3,2 % (bb), ), A. variabilis Blume. (walur) 2,52 % (bb), Colocasia esculenta (L.) Schott. (bentul) 2,4 % (bb), Alocasia macrorrhiza (L.) Schott. (sente) 1,3 % (bb) dan terendah Xanthosoma sp.(endro/mbote kuning) 0,64 % (bb). Kata kunci: Araceae, Sumber  Glukomanan, Jawa Timur
Variant Clustering Analysis Amorphophallus muelleri Blume Found in East Java Based on Molecular Marking CslA Mannan Synthase Encoder with PCR-RFLP Technique Novie Ary Priyanti; Arik Arubil; Laras Estri Arumningtyas; Rodiyati Azrianingsih
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1397.82 KB) | DOI: 10.21776/ub.natural-b.2013.002.02.4

Abstract

Amorphophallus muelleri Blume is a ubiquotus plant found all over Indonesia and has the potential to be food substitute due to its high glucose content. This research was conducted to investigate the porang’s clustering based on CslA gene glucose coding by using PCR-RFLP technique. Porangs used as the subjet of this study were obtained from 4 different locations in East Java, wich are : (1) Nganjuk, (2) Madiun, (3) Blitar and (4) Jember, analysed by morphological characteristic and molecular traits. Morphological analysis showed that porang derived based on their stalk pattern. BlitarI, JemberI, MadiunI variant have large prismatic stalk patterns, Jember1 and Nganjuk2 have a tight prismatic stalk pattern. Glucomannan content analysis showed that highest content is from Blitar2 5,469% and the lowest is Madiun2 0,2313%. The amplification of CslA gene sequence using primary AkCslA680F and AkCslA1288R showed that CslA gene sequence was amplified on 200 bp. The cutting by using HaeIII restrictive enzyme produced two monomorphic band on 1000 bp and 2000 bp, and 8 polymorphic band on 100 bp-400 bp. The result of this analysis showed that porang had high diversity. The analysis of among variants clustering based on their molecules data showed that grouping fell into 3 clusters, which were: cluster I (JemberI, BlitarI and MadiunI variants), cluster II (Jember2, Blitar2 and Nganjuk2 variants) and cluster III (Blitar2 variant). The shortest distance was found between JemberI and BlitarI variants, while the furthest was found in Blitar2 variants. 
Inventory of Medicinal Plants in Kampung Adat Urug, Urug Village, Sukajaya District, Bogor Regency Muhammad Qais Izzuddin; Rodiyati Azrianingsih
Natural B, Journal of Health and Environmental Sciences Vol 3, No 1 (2015)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (420.109 KB) | DOI: 10.21776/ub.natural-b.2015.003.01.11

Abstract

This research purposed to find out species, preparation and uses of medicinal plants in Kampung Adat Urug. Ethnobotanical survey was conducted from November 2013 until May 2014 using interview (semi-structured) and observation activities. The respondents were determinated by snowball sampling with the head of Kampung Adat Urug as a key informant. The observation was conducted for documenting medicinal plants based on respondent recomendation. Data gained from this activity was qualitatively described. Medicinal plants used by the Kampung Urug were 53 species singly and 5 herbs. Those species belonged 27 families. The most recomended medicinal plants were Cardamom and Bitung Bamboo. Those plants often to treat cough with dringking extraxted water from trunk (dituak). Bitung Bamboo shoot soup is used to prevent aging. In Kampung Adat Urug, traditional midwife often use medicinal plants to make herbs for healing any diseases, such as headache, stomachache, kidney problem, and also increasing stamina for adult and women after pregnant. Medicinal plants and herbs which were recomended by respondents were adequate treatment for any diseases because they have evidences between ethic and emic  
ANATOMI DAN HISTOKIMIA ORGAN GENERATIF AMORPHOPHALLUS MUELLERI Dwi Gusmalawati; Serafinah Indriyani; Rodiyati Azrianingsih
Floribunda Vol. 4 No. 7 (2013)
Publisher : PTTI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.833 KB) | DOI: 10.32556/floribunda.v4i7.2013.110

Abstract

Porang is a tuber-producing plants and can be used as foodstuffs, cosmetics, pharmaceuticals and other industries. Porang growth cycle consists of vegetative phase and generative phase. The vegetative phase, when tuber produces petiole and lamina, while the generative phase, when tuber produces flower, fruit, and seeds. The aim of this study was to characterize the anatomy and histochemistry of porang generative organs. This research was conducted on the observations of anatomical characters, with the making of semi-permanent preparations and histochemical observations using fresh preparations. The results of the study showed that the surface of the adaxial and abaxial of the spathe structure of the epidermis was different, superior ovary, hemiantropus ovule, and bilobus anthers, each consisting of two loci. Porang fruit is a berry, thin pericarp with parenchymatous tissue that has a raphide sac and druse. Porang seed has thick testa and thin tegmen, as well as have the raphide sac. Based on testing of histochemistry, porang seeds contain starch, protein and lipid.
Struktur dan Perkecambahan Serbuk Sari Pada Porang (Amorphophallus Muelleri Blume) Dwi Gusmalawati; Serafinah Indriyani; Rodiyati Azrianingsih; Zainal Abidin
G-Tech: Jurnal Teknologi Terapan Vol 7 No 2 (2023): G-Tech, Vol. 7 No. 2 April 2023
Publisher : Universitas Islam Raden Rahmat, Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (415.171 KB) | DOI: 10.33379/gtech.v7i2.2449

Abstract

This study aims to analyze the structure and germination of pollen in porang. Pollen structure and germination were observed microscopically. Pollen germination observations were carried out three days before anthesis (M-3), two days before anthesis (M-2), one day before anthesis (M-1), anthesis (M) to four days after anthesis (M+4). Pollen in porang has a dimorphic structure (X and Y structures: monad and compound units). Structure X with axis length (P)= 30-50 µm, equatorial diameter (E)= 16.67-41.67 µm, and index P/E= 1.19-1.79, while structure Y with P= 50-84 µm, E= 34-67 µm, and P/E index= 1.25-1.40. Porang pollen was subspheroidal and prolate in shape; the length of the axes of the media and magna types; monocolpate aperture type. The highest viability of the X structure was at M (31.58%), while the Y structure was at M+1 (21.76%). M+3 viability begins to decline, so porang artificial pollination is appropriate when pollen viability is high (M or not more than M+3).
Uji Viabilitas Biji dan Pertumbuhan Porang (Amorphophallus muelleri Blume) dari Beberapa Variasi Bagian Tongkol Dwi Gusmalawati; Serafinah Indriyani; Rodiyati Azrianingsih; Zainal Abidin
G-Tech: Jurnal Teknologi Terapan Vol 7 No 4 (2023): G-Tech, Vol. 7 No. 4 Oktober 2023
Publisher : Universitas Islam Raden Rahmat, Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33379/gtech.v7i4.3042

Abstract

Tujuan dari penelitian ini yaitu untuk menentukan viabilitas biji dan pertumbuhan porang dari beberapa variasi bagian tongkol. Studi ini menggunakan Desain Acak Lengkap. Ulangan dilakukan sebanyak tiga kali dengan tiga perlakuan (bagian tongkol: ujung, tengah dan pangkal). Persentase biji bertunas menentukan viabilitas biji. Parameter pertumbuhan yang diukur adalah tinggi tanaman, penampang tangkai daun, penampang tajuk, ketebalan, penampang, dan bobot umbi. Variasi biji dari bagian tongkol buah tidak berpengaruh pada viabilitas biji porang, tetapi berpengaruh pada ukuran tinggi tanaman, penampang tangkai daun, penampang tajuk, ketebalan umbi, penampang umbi, dan bobot umbi. Biji dari bagian tengah dan pangkal menghasilkan parameter pertumbuhan yang terbaik daripada ujung, terutama pada umbi yang dipanen. Umbi yang paling berat adalah 1,79±1,15 g (dari biji bagian pangkal), kemudian 1,72±1,08 g (tengah), dan 1,09±0,77 g (ujung). Biji yang terbaik untuk digunakan sebagai sumber benih yaitu biji yang diambil dari bagian tengah dan pangkal.
Ethnobotany of Medicinal Plants as a Wellness Tourism Industry in Kalipuro- Gombengsari Village, Banyuwangi Regency Ikbal, Mohammad; Azrianingsih, Rodiyati; Hakim, Luchman
Journal of Indonesian Tourism and Development Studies Vol. 11 No. 2 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jitode.2023.011.02.04

Abstract

Natural resources such as medicinal plants are important in the formation of wellness tourism development. This study aims to determine medicinal plants, their utilization, and necessary strategies for their development in the wellness tourism industry. The research methodology involved data collection, observation, and documentation. The study had 40 respondents over 45 years old, selected based on their experience in traditional medicine. The study involved two types of informants: 13 key informants and 27 non-key informants. Data were analyzed using UVs, ICS, and SWOT. It was found that the informants in Kalipuro-Gombengsari Village utilized 106 species of medicinal plants. Cinnamomum burmannii (UV = 8), Centella asiatica (UV = 6), and Arenga pinnata (UV = 5) were the most commonly used plants. The findings revealed 75 prevalent diseases in the community, with stomach-related issues being the most common, treatable with 19 species of medicinal plants. Zingiber officinale, Carica papaya, and Musa paradisiaca were the top three plants with the highest ICS values, scoring 100 due to their numerous benefits. Plant conservation, education, and cultural preservation in Kalipuro-Gombengsari can be supported by the preservation of the Digital House and Kembang Galengan as centers for the development of wellness tourism. Keywords: Ethnobotany, medicinal plants, wellness tourism.
PENINGKATAN PENGETAHUAN DAN KETERAMPILAN PELAKU USAHA DALAM MENGIMPLEMENTASIKAN PROSES PRODUK HALAL Leksono, Amin Setyo; Herawati, Herawati; Azrianingsih, Rodiyati; Qurotaayuina, Riska Putri
JURNAL PENGABDIAN PAPUA Vol 8 No 3 (2024)
Publisher : LPPM Uncen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31957/jpp.v8i3.4186

Abstract

The community service activity in the Kasembon area was focused on the community of Sukosari Village, Kasembon District, which has significant potential in product development through local SMEs. However, the lack of knowledge and skills in halal production and certification has become an obstacle. Therefore, this activity aims to improve the knowledge and skills of business actors in halal product processes and slaughtering techniques. The methods used include site surveys, involving the Kasembon Business Destination Association (PADUKA), followed by education and mentoring. The activity was attended by 20 participants, consisting of business actors and prospective halal slaughterers. Based on the results, it can be concluded that education/presentation increased the participants' knowledge, motivation, and interest in implementing halal product processes and certification in Kasembon SMEs. The participants' initial knowledge regarding halal product process criteria was quite good, but many were still unaware of the differences between self-declaration and regular certification, the Halal Product Assurance System Manual Document, halal slaughtering processes, and marketing strategies. With counseling and training, participants' knowledge and awareness increased significantly. As a result, participants' understanding increased from 15-60% to 30-50%, with a satisfaction rate of 50% being very satisfied, 45% satisfied, and 75% committed to following up, developing, and supporting halal product certification in Kasembon. Keywords: halal product; halal certification; UMKM.
DNA QR Code Using Internal Transcribed Spacer 1 (ITS1) Region of Commercial Cucumber Varieties (Cucumis sativus L.) Turhadi, Turhadi; Padmana, I Made Dhena Radya; Azrianingsih, Rodiyati
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n1.p1-7

Abstract

DNA Quick Response (DQR) is an accurate, efficient, fast, and cost-effective alternative to PCR-based or other sequencing methods. DQR is the method integrated seamlessly with digital systems. The DQR could be apply as effective approach to preventing seed counterfeiting, for example in cucumber. This study aimed to identify DNA barcodes based on the ITS1 region and develop DQR for commercial cucumber varieties in Indonesia. Two cucumber varieties, namely cv. Mars and cv. Makka were used. The genomic DNA was extracted using commercial DNA purification kit. The DQR for these two cucumber varieties were developed based on experimental data from the ITS1 region. The efficiency and characterization of ITS1 region were evaluated through homology analysis using BLAST-NCBI and DQR development. The DQR of cucumber cv. Mars and cv. Makka based on ITS1 region were successfully developed. The length of the ITS1 region encrypted in the QR code was 496 bp and 437 bp for cv. Mars and cv. Makka, respectively. The A-T and G-C proportions for cv. Mars were 45.2% and 54.8%, respectively, while for cv. Makka, the A-T and G-C proportions were 42.6% and 57.4%, respectively. Our findings are valuable for genetic labeling and authentication of horticultural crops, especially cucumber.
Molecular characterization of genus Tor from Indonesia based on 16S rRNA Rahayu, Dwi Anggorowati; Nugroho, Endik Deni; Azrianingsih, Rodiyati; Kurniawan, Nia
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 7 No. 01 (2022): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v7i01.1880

Abstract

Genus Tor is Indonesia's endangered local fish however, the reports on this species from South East Asia is currently limited. This study conducted to characterize molecular genetics and phylogenetic relationship of Tor fish from East Java, West Kalimantan, Padang and North Sumatra from BPBAT Bogor collection using a partial sequence of mtDNA 16S rRNA. A total of ten samples of Genus Tor were collected, then identified based on morphological characters, and the identification was confirmed using molecular data. PCR amplicons at 542 bp in length. The construction of phylogenetic topology was made based on ML and NJ method using Kimura-2 parameter model. Based on the phylogenetic topology showed that Tor fish from Pasuruan are closely related to Tor duoronensis from Padang with a bootstrap value of 66%, while Tor duoronensis fish from North Sumatra and Tor tambra from West Kalimantan are in separated clusters. This finding also contributes to the differentiate Genus Tor from Indonesia based on polymorphic sites