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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Buletin Tanaman Tembakau, Serat & Minyak Industri Indonesian Green Technology Journal AGRIVITA, Journal of Agricultural Science Buletin Tanaman Tembakau, Serat & Minyak Industri Jurnal HPT (Hama Penyakit Tumbuhan)
Bambang Tri Rahardjo
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Engineering Environmental Science Other

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Isolation and Identification Of Triterpenoid Saponin From Baringtonia asiatica Kurz Seeds. Meity N Tanor; Abdul Latief Abadi; Bambang Tri Rahardjo; Jantje Pelealu
Journal of Tropical Life Science Vol. 4 No. 2 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.4.2.%x

Abstract

The study aimed to identify the content of the triterpenoid saponin compound from Barringtonia asiatica seeds collected from coastal of Malalayang beach, Manado, North Sulawesi. The method of extraction was the maceration with methanol solvent. The identification of the isolate was by thin layer chromatography, column chromatography, and GCMS. The phytochemistry test result of B. asiatica seed extract contained the compounds of alkaloid, saponin, and tannin. Then, the compound was separated by a thin layer chromatography method with a solvent system of methanol:chloroform:water. It produced three nodes that were spread around polar (Rf 0.24), semipolar (Rf 0.6) and non-polar (Rf 0.78) areas. Meanwhile, the process of column chromatography could only separate two chemical components namely semipolar (Rf 0.6) and polar (Rf 0.76). The identification with GCMS resulted in three compounds of Triterpenoid saponins, namely 2.4-bis-(1.1-dimethyl ethyl); 4-Dodecylphenol; and 2.6 bis-(1.1-dimethyl ethyl-4-methyl).
MECHANISM OF INFECTION Spodoptera litura Nucleopolyhedrosis Multiple Virus (SpltMNPV) ON MIDGUT EPITHELIAL CELL ARMY WORM (Spodoptera litura) Observed by TEM Mahanani Tri Asri; Siti Rasminah Chaelani; Bambang Tri Rahardjo; Sutiman Bambang Sumitro
AGRIVITA Journal of Agricultural Science Vol 35, No 1 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i1.115

Abstract

Spodoptera litura is one of  agricultural crop pests. They are resistant to chemical insecticides. One of arternate biological control is Spodoptera litura Nucleopolyhedrosis Multiple Virus (SpltMNPV) Penelitian ini dilakukan untuk mengetahui bagaimana mekanisme SpLtMNPV dalam menginfeksi sel epithel midgut larva S. The research was conducted to determine how SpltMNPV infected midgut epithelial cells of S. litura secara in vitro . litura larvae in vitro.  The mechanism of infection was observed by a transmission electron microscopy (TEM).  The result of this observation showed that infecting midgut cell by  SpltMNPV involved 5 phases, they are, 1) the attachment of  SpltMNPV at the membrane of suitable host, 2) the penetration, formation of channels and release of protein envelope, 3) the biosynthesis of virus components in the cell nucleus, 4) the assembling of virus components, and 5) the releasing of MNPV/multiplenucleocapsid through budding. Keywords: mechanisms of infection, Spodoptera litura multiple nucleopolyhedrosis virus, midgut army worm larvae cells.
Co-Authors Abdul Latief Abadi Akhmad Rizali Arifin Noor Sugiarto Bachtiar Rachmad Sugiyono Bagyo Yanuwiadi Bobby Polii Budi Pratama Exa Ricky Choirul Putra Hagus Tarno Jantje Pelealu Kafif Andani Kristiana Sri Wijayanti Ludji Pantja Astuti Mahanani Tri Asri Marianus Marianus Meity Nelltje Tanor Muhammad Indar Pramudi Retno Dyah Puspitarini Siti Rasminah Chaelani Sumeru Ashari Sutiman Bambang Sumitro Syahrullah Bagus Harmana Toto Himawan