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All Journal PROSIDING SEMINAR NASIONAL Biogenesis: Jurnal Ilmiah Biologi Jurnal Kesehatan Masyarakat Andalas (Andalas Journal of Public Health) Prosiding Seminar Biologi Indonesian Journal of Biotechnology Jurnal Teknologi Laboratorium Journal of Health (JoH) Adi Widya : Jurnal Pengabdian Masyarakat Martabe : Jurnal Pengabdian Kepada Masyarakat ABDIMAS SILIWANGI Jurnal Biomedika Sang Pencerah: Jurnal Ilmiah Universitas Muhammadiyah Buton Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Jurnal Ilmiah Permas: Jurnal Ilmiah STIKES Kendal Jurnal Nasional Teknologi Terapan Nusantara Science and Technology Proceedings Jurnal Mitra Kesehatan Jurnal Biosense Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) J.Abdimas: Community Health SEMINAR NASIONAL PENELITIAN DAN PENGABDIAN KEPADA MASYARAKAT Jurnal Vokasi Kesehatan Gema Kesehatan Journal Fatmawati Laboratory & Medical Science
Fitri Nadifah
Program Studi D3 Analis Kesehatan STIKES Guna Bangsa Yogyakarta
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Potensi Ekstrak Serai Wangi (Cymbopogon nardus (L.) Rendl.) sebagai Larvasida Nyamuk Aedes aegypti Fitri Nadifah; Desto Arisandi; Siti Mahmuda
Seminar Nasional Penelitian dan Pengabdian Kepada Masyarakat 2022: Prosiding Seminar Nasional Penelitian dan Pengabdian Kepada Masyarakat (SNPPKM 2022)
Publisher : Universitas Harapan Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.455 KB) | DOI: 10.35960/snppkm.v2i1.1170

Abstract

Demam Berdarah Dengue (DBD) merupakan salah satu permasalahan kesehatan masyarakat utama di seluruh daerah tropis, termasuk Indonesia. Nyamuk Aedes aegypti merupakan perantara utama penularan penyakit DBD. Salah satu cara untuk mengendalikan jumLah nyamuk adalah menggunakan larvasida. Serai wangi (Cymbopogon nardus (L.) Rendle) merupakan tanaman yang tersebar di banyak wilayah di Indonesia dan berpotensi sebagai larvasida alami karena mengandung sejumLah zat aktif, terutama sitronela dan geraniol. Tujuan penelitian ini adalah untuk mengetahui kematian larva nyamuk Ae. aegypti dengan perlakuan berbagai konsentrasi ekstrak batang serai wangi. Penelitian ini bersifat deskriptif menggunakan ekstrak serai wangi dengan konsentrasi 4%, 8%, 12%, 16% dan 20%. Sebagai kontrol negatif, digunakan air keran. Dua puluh larva nyamuk Ae. aegypti digunakan sebagai subyek penelitian pada masing-masing perlakuan. Pengamatan kematian larva dilakukan setiap 30 menit sekali selama 2 jam. Kontrol negatif dan konsentrasi terendah ekstrak tidak mengakibatkan kematian larva (0%), sedangkan konsentrasi 8% menghasilkan 40% kematian larva. Pada konsentrasi ekstrak 12%, 16% dan 100%, seluruh larva mati setelah perlakuan 2 jam. Konsentrasi ekstrak minimum yang efektif dapat mengakibatkan 50% kematian larva adalah 12%. Dari penelitian ini dapat disimpulkan bahwa ekstrak batang serai wangi memiliki potensi sebagai larvasida dan menggantikan larvasida konvensional dengan mengikuti standar etika yang telah ditetapkan.
Daya Hambat Minyak Atsiri Rimpang Kunyit terhadap Pertumbuhan Candida Albicans In Vitro Fitri Nadifah; Nurlaili Farida Muhajir; Fitri Retnoningsih
Jurnal Vokasi Kesehatan Vol 4, No 1 (2018): Januari 2018
Publisher : Poltekkes Kemenkes Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (237.5 KB) | DOI: 10.30602/jvk.v4i1.124

Abstract

Abstract: Inhibition Activity Of Turmeric Essential Oil Against The Growth Of Candida Albicans. Turmeric has rhizomes which contain essential oils and curcuminoid that has anti-fungal activity. The purpose of this research was to know the influence of essential oil of turmeric Rhizome (Curcuma domestica Val.) against the growth of Candida albicans in vitro. This was an experimental research with diffusion method. Essential oil obtained through distillation methods and the concentration used was 20%, 40%, 60%, 80% and 100%. The treatment was repeated three times. Anova test was used. Inhibition zone is formed at a concentration of 20%, 40%, 60%, 80%, 100% were 6,16 mm, 6,50 mm, 6,66 mm, 7,00 mm and 7.83 mm, respectively Results in analysis by one way ANOVA showed there was a significant difference in the variance of essential oil concentration against the inhibition zone (p< 0.05).Abstrak: Daya Hambat Minyak Atsiri Rimpang Kunyit Terhadap Pertumbuhan Candida Albicans In Vitro. Kunyit merupakan  salah satu rimpang yang diketahui memiliki kandungan minyak atsiri serta curcuminoid yang bersifat anti jamur. Tujuan penelitian ini untuk mengetahui pengaruh minyak atsiri rimpang kunyit (Curcuma domestica Val.) terhadap pertumbuhan Candida albicans secara in vitro. Jenis penelitian ini adalah penelitian eksperimental dengan metode difusi cara sumuran. Minyak atsiri didapatkan melalui   metode destilasi dan konsentrasi  yang digunakan adalah 20%, 40%, 60%, 80% dan 100%. Perlakuan diulang sebanyak tiga kali. Hasil penelitian di analisis dengan Anova. Hasil zona hambat yang terbentuk pada konsentrasi minyak atsiri 20%, 40%, 60%, 80%, 100% berturut-turut adalah 6,16 mm, 6,50 mm, 6,66 mm, 7,00 mm,  dan 7,83 mm. Hasil analisis dengan one way anova menunjukkan terdapat perbedaan yang signfikan pada berbagai konsentrasi minyak atsiri terhadap zona hambat yang dihasilkan (p<0,05). Dari penelitian ini dapat disimpulkan bahwa berbagai konsentrasi minyak atsiri rimpang kunyit dapat menghambat pertumbuhan Candida albicans. Kemampuan ini berpotensi dikembangkan lebih lanjut sebagai alternatif dalam pengobatan terhadap penyakit karena infeksi Candida albicans.
IDENTIFIKASI IMMUNOGLOBULIN MIU (IgM) IMMUNOGLOBULIN GAMMA (IgG) ANTI SALMONELA PADA SERUM PASIEN DEMAM TIFOID DI PUSKESMAS GODEAN II, SLEMAN, YOGYAKARTA Yuliana Prasetyaningsih; Fitri Nadifah; Desto Arisandi; Dieta Dieon Saputri
GEMA KESEHATAN Vol. 12 No. 2 (2020): Desember 2020
Publisher : POLTEKKES KEMENKES JAYAPURA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47539/gk.v12i2.134

Abstract

Widal examination is a serological method to diagnose typhoid fever used by Godean II Public Health Center, Sleman Regency, Yogyakarta Special Region (DIY). However, this method is difficult to hold onto because there is no agreement on agglutination standards. A positive Widal test result is not necessarily typhoid fever. Therefore, the results really need to be confirmed by other supporting examinations. IgM anti Salmonella IgG rapid test is one of the serological tests to support the diagnosis of typhoid fever which has better sensitivity and specificity to handle specific antibodies against Salmonella typhi in serum. This study aims to examine the results of the serological test of Immunoglobulin Miu (IgM) Anti Salmonella gamma immunoglobulin (IgG) on positive widal samples at Godean II Public Health Center and to see the lowest positive widal titers IgM antisalmonella in the sample. This research is a descriptive study with accidental sampling technique. The method of examination used a rapid test immunochromatography to examine IgM IgG anti Salmonella typhi on positive widal serum samples at Puskesmas Godean II, Sleman, DIY. The results showed that 21.7% of the samples were positive IgG negative IgM; 8.7% of IgM samples were positive IgG positive, and 69.6% IgM negative IgG negative. Anti Salmonella IgM in widal positive samples appeared at titer 320. Anti Salmonella typhi IgM and IgG were detected in positive widal samples at Godean II Public Health Center. Immunoglobulin Miu (IgM) appears at titer 320.
Overabundance of Lactobacillus iners in the Urogenital Microbiome of a Female Patient with Urinary Tract Infection Fitri Nadifah; Wayan Tunas Artama; Budi Setiadi Daryono; Endah Retnaningrum
Nusantara Science and Technology Proceedings 2nd Bioinformatics and Biodiversity Conference
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/ nstp.2022.2108

Abstract

The microbiome that inhabits the human body plays a role not only in maintaining health but also in causing diseases, such as urinary tract infections (UTIs). This occurs due to changes in the host's body and causes changes in the composition of the microbiota that make up the microbiome. This case report contains the results of 16S rRNA gene sequencing in the urine specimens of a 41-year-old female patient diagnosed with UTI. Illumina MiSeq was carried out for 16S rRNA gene sequencing in the V3-V4 hypervariable region. The 16S rRNA sequence yielded a total of 98,754 high-quality reads with 283 species and 299 operational taxonomic units (OTUs) identified. Based on the sequencing results, we found an overabundance (93%) of Lactobacillus iners as the most abundant and dominant taxa. Lactobacillus iners is one of the bacteria that is difficult to culture. However, with the 16S rNA gene sequencing technology, these bacteria can be detected along with their abundance in the structure of the urogenital microbiome. Bacterial overabundance may have occurred as a result of changes in the host ecology, resulting in UTI clinical symptoms. This result indicates that, in addition to maintaining the health of the urogenital organs, L. iners can also be the causative agent of UTI. An ecological perspective appears to be necessary to determine the composition and function of the microbiota, especially in patients with UTI. The data set has been sent via NCBI as a Sequence Read Archive (SRA).
AKTIVITAS ANTIBAKTERI EKSTRAK DAUN LIDAH MERTUA (Sansivieria tirfasciata var.laurentii) TERHADAP BAKTERI Klebsilla pneumonia Fitriani, Audiah; Sawitri, Nining Eka; Khatrina, Syahdapuan Dara; Nadifah, Fitri; Arisandi, Desto
Journal of Indonesian Medical Laboratory and Science Vol 5 No 2 (2024): In Progress
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v5i2.217

Abstract

Background: Pneumonia is an acute infection of the lower respiratory tract characterized by coughing and shortness of breath caused by Klebsiella pneumoniae bacteria. Sansevieria contains flavonoids, saponins, alkaloids, and steroids known to have antibacterial properties that can destroy the bacterial cell walls.  Objectives: This study aimed to determine the antibacterial activity of Sansevieria leaf extract against the growth of K. pneumoniae.  Materials and Methods: Detection of antibacterial activity of Sansevieria was conducted with disc-diffusion test. The presence of antibacterial activity is indicated by the appearance of inhibition (clear) zone after applying the extract at a concentration of 60-100% to the culture medium. As a positive control, the antibiotic chloramphenicol was used.  Results: The higher the extract concentration, the higher the inhibition zone formed. The inhibition zone formed from extract concentrations of 60%, 70%, 80% is classified as medium (d = 8; 8.5; 9 mm). Meanwhile, the inhibition zones at concentrations of 90% and 100% are 10.5 and 13.5 mm and are classified as strong. The clear zone of the positive control was 29 mm (very strong). Conclusions: Sansevieria leaf extract has the potential to inhibit the growth of K. pneumoniae bacteria.
Potential of Hibiscus (Hibiscus rosa-sinensis L.) Extract as a Substitute for Eosin in Hematoxylin-Eosin Histological Staining Nadifah, Fitri; Prasetyaningsih, Yuliana; Muhajir, Nurlaili Farida; Murtiningrum, Sri; Ohoiwutun, Clara Shinta D.P; Puspita, Elisabet Della
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 1 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i1.368

Abstract

Staining is an important stage in preparing microscopic preparations so that the histological structure can be observed clearly. Hematoxylin-Eosin is the most widely used dye. Unfortunately, eosin is a synthetic chemical compound that is carcinogenic. To reduce the health and environmental impacts of eosin compounds, it is important to look for alternative dyes from natural ingredients. Hibiscus (Hibiscus rosa-sinensis L.) is a plant known to contain anthocyanins which can be used as eosin substitutes. This study aims to determine the potential of hibiscus extract as a substitute for eosin in histological staining. In this study, unstained liver tissue preparations from mice (Mus musculus) were used. Hibiscus extract dye was compared with eosin as a control. The color quality of the tissue preparations was used as an experimental parameter. The results of the microscopic assessment showed that liver tissue stained with Hibiscus extract at concentrations of 60% and 80% was less good than eosin. However, because tissue can stain reddish, hibiscus extract has the potential to be used as an eosin substitute. The right extraction method may be able to produce intact anthocyanin so that it can color the tissue optimally.
Uji Cemaran Bakteri Coliform pada saus sambal jajanan Bakso Tusuk Sepanjang Jalan Malioboro Yogyakarta Prasetyaningsih, Yuliana; Nadifah, Fitri; Marthaning Tyas, Wina
Jurnal Fatmawati Laboratory & Medical Science Vol 1 No 1 (2021): Bacteriology dll
Publisher : POLTEKKES KEMENKES BENGKULU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33088/flms.v1i1.185

Abstract

Saus sambal sering disajikan sebagai pelengkap berbagai macam makanan lainnya, contohnya jajanan bakso tusuk yang sering dikonsumsi oleh anak sekolah maupun orang dewasa. Coliform merupakan golongan bakteri yang hidup di saluran pencernaan manusia dan hewan. Adanya bakteri coliform di dalam makanan atau minuman menunjukkan kemungkinan adanya mikroorganisme yang bersifat enteropatogenik dan/atau toksigenik yang berbahaya bagi kesehatan. Penelitian ini bertujuan untuk mengetahui ada atau tidaknya bakteri coliform dan jumlah bakteri coliform pada saus sambal jajanan bakso tusuk sepanjang jalan Malioboro Yogyakarta dan untuk mengetahui mutu saus sambal jajanan bakso tusuk sepanjang jalan Malioboro Yogyakarta berdasarkan standar SNI 7388:2009. Jenis Penelitian observasi yang bersifat deskriptif bertujuan untuk mengetahui ada atau tidaknya bakteri coliform dan mutu bakteri coliform pada saus sambal jajanan bakso tusuk sepanjang jalan Malioboro Yogyakarta. Hasil Penelitian menunjukkan bahwa dari 9 sampel ada bakteri coliform pada sampel pedagang 5 (4 APM/gr), pedagang 8 (2 APM/gr), pedagang 9 (5 APM/gr) dan tidak ada bakteri coliform pada sampel pada pedagang 1 (0 APM/gr), pedagang 2 (0 APM/gr), pedagang 3 (0 APM/gr), pedagang 4 (0 APM/gr). Dari hasil penelitian yang diperoleh dapat disimpulkan bahwa 100% saus sambal yang digunakan oleh pedagang bakso tusuk di sepanjang jalan Malioboro Yogyakarta memenuhi SNI 7388:2009
Overabundance of Lactobacillus iners in the Urogenital Microbiome of a Female Patient with Urinary Tract Infection Fitri Nadifah; Wayan Tunas Artama; Budi Setiadi Daryono; Endah Retnaningrum
Nusantara Science and Technology Proceedings 2nd Bioinformatics and Biodiversity Conference
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/ nstp.2022.2108

Abstract

The microbiome that inhabits the human body plays a role not only in maintaining health but also in causing diseases, such as urinary tract infections (UTIs). This occurs due to changes in the host's body and causes changes in the composition of the microbiota that make up the microbiome. This case report contains the results of 16S rRNA gene sequencing in the urine specimens of a 41-year-old female patient diagnosed with UTI. Illumina MiSeq was carried out for 16S rRNA gene sequencing in the V3-V4 hypervariable region. The 16S rRNA sequence yielded a total of 98,754 high-quality reads with 283 species and 299 operational taxonomic units (OTUs) identified. Based on the sequencing results, we found an overabundance (93%) of Lactobacillus iners as the most abundant and dominant taxa. Lactobacillus iners is one of the bacteria that is difficult to culture. However, with the 16S rNA gene sequencing technology, these bacteria can be detected along with their abundance in the structure of the urogenital microbiome. Bacterial overabundance may have occurred as a result of changes in the host ecology, resulting in UTI clinical symptoms. This result indicates that, in addition to maintaining the health of the urogenital organs, L. iners can also be the causative agent of UTI. An ecological perspective appears to be necessary to determine the composition and function of the microbiota, especially in patients with UTI. The data set has been sent via NCBI as a Sequence Read Archive (SRA).
PENYULUHAN PENCEGAHAN DEMAM BERDARAH DENGUE DAN PEMBUATAN SPRAY ANTI-NYAMUK BERBAHAN SERAI WANGI BAGI MASYARAKAT PADUKUHAN NOLOGATEN SLEMAN Nadifah, Fitri; Fuadah Hidayat, Tsamrotul; Velisshia, Velisshia; Clara Pattirane, Evelin; Famela Adelena, Fitria; Maloko, Seprianto; Melani Lesmono, Inez; Nindhea Ferliza, Yoan; Dewi Rahadian, Titah; Arisandi, Desto
Martabe : Jurnal Pengabdian Kepada Masyarakat Vol 8, No 2 (2025): MARTABE : JURNAL PENGABDIAN KEPADA MASYARAKAT
Publisher : Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jpm.v8i2.796-800

Abstract

World Health Organization (WHO) menyatakan bahwa demam berdarah dengue (DBD) masih menjadi satu dari 20 ancaman kesehatan di dunia. Padukuhan Nologaten Sleman adalah Padukuhan dengan kepadatan penduduk yang tinggi. Data dari Puskesmas Depok III Sleman menunjukkan bahwa tercatat 4 kasus DBD di wilayah RT 06 Padukuhan Nologaten per-Maret 2024. Kegiatan pengabdian masyarakat ini dilakukan dalam bentuk Penyuluhan “Kenali dan Cegah Demam Berdarah Dengue” disertai edukasi pembuatan spray anti-nyamuk berbahan serai wangi. Pembuatan spray ini sangat mudah karena serai wangi dapat diperoleh di sekitar rumah. Kegiatan ini kami nilai berhasil, dilihat dari antusiasme peserta selama mengikuti kegiatan.   
KENALI DAN CEGAH DEMAM BERDARAH DENGUE SEBAGAI UPAYA PENCEGAHAN DAN PENANGGULANGAN PENYAKIT Arisandi, Desto; Bulu, Martinus Tagu; De Jesus, Maria Carolina; Saraswati, Farikhatun Nisaa; Mosa, Rambu Pedi; Nono, Arianto; Bolosan, Fabio Andre; Dapa Ole, Ericson Umbu Robaka; Nadifah, Fitri
Adi Widya : Jurnal Pengabdian Masyarakat Vol 9 No 1 (2025): Adi Widya: Jurnal Pengabdian Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33061/awpm.v9i1.11824

Abstract

Dengue Hemorrhagic Fever (DHF) is transmitted through the bites of Aedes aegypti and Aedes albopictus mosquitoes. Efforts that can be made to reduce the incidence of dengue fever are through the 3M plus movements (draining, burying and covering) and carrying out preventive activities such as sprinkling larvicide powder, using mosquito repellent, and planting mosquito repellent plants. The aim of this community service is to increase public knowledge about recognizing and preventing dengue fever as an effort to prevent and control the disease. The activities were carried out at Padukuhan Nologaten, Caturtunggal Village, Sleman Regency, Yogyakarta Province in May-June 2024. The activities carried out include situation analysis, preparation, implementation, monitoring and evaluation, and analysis and reporting. The participants of this activity numbered 37 people with the majority aged 46-65 years (48%) and college education background (48%). The average level of community knowledge about the prevention and control of dengue fever before counseling (pre-test) was good (89%) and sufficient (11%) and after counseling, the level of community knowledge increased to good (95%).
Co-Authors Anggraeni Anwarudin, Anwarudin Arum Nuryati Bayu Nur Khotib Bayu Nur Khotib, Bayu Nur Bolosan, Fabio Andre Budi Setiadi Daryono BUDI SETIADI DARYONO Budi Setiadi Daryono Bulu, Martinus Tagu Clara Pattirane, Evelin Dani Setiawan Dapa Ole, Ericson Umbu Robaka De Jesus, Maria Carolina Desto Arisandi Dewi Rahadian, Titah Diah Irawati Dian Wuri Astuti Dian Wuri Astuti, Dian Dieta Dieon Saputri Endah Retnaningrum Endah Retnaningrum Endah Retnaningrum Famela Adelena, Fitria Farida Muhajir, Nurlaili Farida Muhajir, Nurlaili Fitri Nadifah Fitri Retnoningsih Fitriani, Audiah Fuadah Hidayat, Tsamrotul Ika Susilowati Indah Yuliana Barekola Lamablawa Indah Yuliana Barekola Lamablawa, Indah Yuliana Barekola Islami Burhanudin Khatrina, Syahdapuan Dara Lisa Susanti Lisa Susanti, Lisa Liza Novia Lani Maloko, Seprianto Maria D. Owa Lobo Maria Yasintha Bhoga Marthaning Tyas, Wina May Tri Mulyani Melani Lesmono, Inez Merry Elisabeth Mosa, Rambu Pedi Nindhea Ferliza, Yoan Nono, Arianto Nurlaili Farida Muhajir Nurlaili Farida Muhajir, Nurlaili Farida Ohoiwutun, Clara Shinta D.P Puspita, Elisabet Della Reni Aqwil Masithah Retnoningsih, Fitri Sanusi , Kristi Elisabeth Saraswati, Farikhatun Nisaa Sawitri, Nining Eka Siti Fatimah Siti Mahmuda Sofvirah R. Sohy Sofvirah R. Sohy, Sofvirah R. Sri Murtiningrum, Sri SRI RAHAYU Susliyanti, Maria Trisno Agung Wibowo Urfiyah Lisa Azizah, Urfiyah Lisa Velisshia, Velisshia Vixa Meindara Wayan Tunas Artama Wayan Tunas Artama Wayan Tunas Artama Yola Ramadhani Yuliana Prasetyaningsih Yuliana Prasetyaningsih Yuliana Prasetyaningsih Yuliana Prasetyaningsih Yuliana Prasetyaningsih Yuliana Prasetyaningsih