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Pengaruh Infusa Daun Sambiloto (Andrographis paniculata Nees) Terhadap Pertumbuhan Bakteri Shigella dysenteriae Secara In Vitro Fitri Nadifah; Siti Fatimah; Indah Yuliana Barekola Lamablawa
Journal of Health (JoH) Vol 1 No 1 (2014): Journal of Health - January 2014
Publisher : LPPM STIKES Guna Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (213.963 KB) | DOI: 10.30590/vol1-no1-p29-31

Abstract

Background: Bacteria Shigella dysenteriae is pathogenic bacterium which cause diarrheal diseases that enter into inside the body through intestine. Shigella dysenteriae constitute one of the gram negative bacterium which causes inflammation on intestinal, pain abdominal, tenesmus, and a bowel movements which accompanied with blood and mucus. Bad impact irational use of synthethic drugs and antibiotics can cause organ damage and germs resistance. Utilization of herbs as traditional medicine evolving rapidly and alternatively used by most. Traditional medicine has relatively low of side effect and affordable prices. Sambiloto (Andrographis paniculata Nees) is a plant which is ordinary used as a traditional medicine that can easily found around Indonesian islands. Sambiloto constitute one of the medicinal plants which is allegedly has the antibacterial effect because its chemical content of flavonoid saponins and tannin. The objective of this study is to know the influence of sambiloto (Andrographis paniculata Nees) leaves infuse against the growth of Shigella dysenteriae bacteria. Method: This is an experimental research using diffusion method. Statistical test who used namely analysis of variant one road (One Way anova). Analysis data were done descriptively and statistically and presented with of tables or graphs. Results: There is influence of sambiloto leaf infusa (Andrographis paniculata Nees) against the growth of Shigella dysenteriae bacteria in vitro with a mean diameter zone of radical on concentrations the smallest 20% is 12 mm and the greatest concentration 100% is 24.3 mm. Conclusion: Infuse leaf has a strong and positive influence in inhibit the growth of bacteria Shigella dysenteriae bacterium.
Helminth Infection of Children in Ngemplak Seneng Village Klaten Fitri Nadifah; Desto Arisandi; Nurlaili Farida Muhajir
Journal of Health (JoH) Vol 3 No 1 (2016): Journal of Health - January 2016
Publisher : LPPM STIKES Guna Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.553 KB) | DOI: 10.30590/vol3-no1-p14-18

Abstract

Helminth infection remains one of health problems of Indonesian people for years. The tropical condition of Indonesian climates with high level humidity has believed to be the one of factors that increase the number of this infection cases. Based of many studies, the number of helminth infections is related to the poor of hygiene and sanitation. This study is conducted to know the percentages of helminth infections among 0-14 years old children in Dusun Ngemplak, Desa Ngemplak Seneng, Klaten and also their personal hygiene with questionaire instruments. To know helminth infection in subjects, we examine faeces sample with simple method and 1% eosin dye. As the results of examination, we found Trichuris trichiura egg in three samples. This might related to the poor of their hygienic behaviors. Some of the behavior such as wash hand before eating and after defecation, use sandal or shoes when playing, consume helminthic drug for last 6 months, and defecate in toilet has shown percentage under 70%. Other behaviors such as cutting and cleaning nails and use a spoon when eating has shown percentage above 70%.
Identification of Malaria Parasites in Chasan Boesoirie General Hospital Ternate East Nusa Tenggara Desto Arisandi; Sofvirah R. Sohy; Fitri Nadifah
Journal of Health (JoH) Vol 3 No 1 (2016): Journal of Health - January 2016
Publisher : LPPM STIKES Guna Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (241.014 KB) | DOI: 10.30590/vol3-no1-p39-44

Abstract

Background: Malaria is still a global health problem especially in tropical countries, such as Indonesia. Based on survey 35% of the Indonesia population were live in endemic areas of malaria, such as Provinces of Maluku, North Maluku, Papua, West Papua, North Sumatra, and East Nusa Tenggara. Malaria is caused by a mosquito bite of female Anopheles sp. containing Plasmodium parasite. Generally it bites humans at night or dawn. Such malaria parasites are Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, and Plasmodium ovale. Malaria is a disease of both highest in Ternate and mostly occur in general hospitals of Chasan Boesoirie General Hospital. This research aims to identify the type of malaria parasites in the Chasan Boesoirie General Hospital Ternate. Method: This is a descriptive research conducted in Chasan Boesoirie General Hospital in Ternate, East Nusa Tenggara in March 2015. Research data obtained include: age, sex, education, and employment. Identification of malaria is carried out based on an examination of the existence of Plasmodium in preparation of thin and thick blood apus. Descriptive analysis using STATA programs for Windows version 12.0 (Stata Corp LP., College Station, TX, USA) are presented in the form of tables and narratives. Result: This research subjects were 69 people suspected with malaria infection. Suspected malaria to be infected by Plasmodium is more are young age group 94%, 88% were males, 100% have a poor education or un-educated, as well as the of 100% were fishermen. As much as 54 people (78%) were infected by Plasmodium parasites consisting of Plasmodium falcifarum 48 people (89%) and Plasmodium vivax 6 people (11%). Conclusion: The incidence of malaria infections in Chasan Boesoirie General Hospital amounted to 78% and the type of infected parasites were Plasmodium falcifarum and Plasmodium vivax.
Efektivitas Infusa Daun Talok (Muntingia calabura) dalam Menghambat Pertumbuhan Bakteri Klebsiella pneumoniae Fitri Nadifah; Desto Arisandi; Siti Fatimah; Dani Setiawan
Jurnal Nasional Teknologi Terapan (JNTT) Vol 2, No 3 (2018): NOVEMBER
Publisher : Penelitian dan Pengabdian Kepada Masyarakat Sekolah Vokasi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (192.005 KB) | DOI: 10.22146/jntt.44933

Abstract

Klebsiella pneumoniae is one of the most bacteria found in urine of Urinary Tract Infection (UTI) Patients. Study in many countries has found that this species become resistant to more than one antibiotics. This research goal is to know the effectiveness of talok (Muntingia calabura) infused water inhibiting the growth of K. pneumoniae. This was an experimental study using K. pneumoniae bacteria isolated from urine samples using diffuse agar method. The concentration of talok infused water was varied from 25-100% and the antibiotics used was cefotaxime, meropenem and chloramphenicol. The inhibition activity was measured by the diameter of inhibition zone. The results show that K. pneumoniae is still give sensitive response to antibiotic treatments. While the inhibition zone from talok infused water treatments can be categorized in middle and strong activity. From this study we can conclude that talok infused water potential in inhibiting the growth of K. pneumoniae. Future study still needed to develop this potential, especially for the bacteria that has been resistant to some antibiotics.
Penyuluhan Perilaku Hidup Bersih Dan Sehat (PHBS) Di Dusun Berjo Wetan Godean Sleman Yogyakarta Dian Wuri Astuti; Fitri Nadifah; Yuliana Prasetyaningsih; Vixa Meindara; Anggraeni; Diah Irawati; May Tri Mulyani
J.Abdimas: Community Health Vol 1 No 1 (2020): J.Abdimas: Community Health - November 2020
Publisher : LPPM STIKES Guna Bangsa Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30590/jach.v1i1.232

Abstract

Clean and healthy living behavior (PHBS) is a step that must be taken to achieve an optimal health degree for everyone. Cultivating a healthy lifestyle in society must be initiated and worked on from oneself. Meanwhile, public awareness of health and hygiene and healthy lifestyles, especially rural communities is still very low. For this reason, presenting counseling is expected to increase public knowledge and awareness of the importance of making clean and healthy living behaviors (PHBS) in daily life so that a better health degree can be realized.
Lactobacillus Composed Urinary Microbiome of Healthy People and Urinary Tract Infection Patients Fitri Nadifah; Wayan Tunas Artama; Budi Setiadi Daryono; Endah Retnaningrum
Sang Pencerah: Jurnal Ilmiah Universitas Muhammadiyah Buton Vol 8 No 4 (2022): Sang Pencerah: Jurnal Ilmiah Universitas Muhammadiyah Buton
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Universitas Muhammadiyah Buton

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (328.812 KB) | DOI: 10.35326/pencerah.v8i4.2196

Abstract

Lactobacillus has long been known to reconstruct the microbiome of the urogenital organs of healthy people. However, changes in lactobacillus abundance are known occurring in urogenital tract infections (UTI) patients. It is also known that there are Lactobacillus species that have specific genomic characteristics and nutritional requirements that cause them to turn into pathogens. Understanding population characteristics and taxa composition that reconstruct urine microbiome is very important for the diagnosis and treatment of urogenital infections. This review provides an update information of metagenomic sequencing to identify the urinary microbiome profile and the role of lactobacilli in healthy and UTI patients.
Characterization of the urogenital microbiome in patients with urinary tract infections Fitri Nadifah; Wayan Tunas Artama; Budi Setiadi Daryono; Endah Retnaningrum
Indonesian Journal of Biotechnology Vol 27, No 3 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.69212

Abstract

Standard microbiological culture techniques can only identify a fraction of the urogenital microbiome. Meanwhile, identifying and characterizing infectious microorganisms are very important for the success of diagnosis and treatments, especially for Urinary Tract Infection (UTI) patients. This study aimed to characterize the urogenital microbiome of UTI patients using 16S rRNA gene sequencing. We sequenced two pooled DNA samples from voided urine of UTI patients (21 females and 13 males). To determine the structure and composition of taxa in the samples, 16S rRNA gene sequencing was performed using the Illumina Mi‐Seq paired‐end platform. The most abundant genera were Burkholderia‐Caballeronia‐Paraburkholderia (71%) followed by Prevotella (33%), Escherichia‐Shigella (24%), Klebsiella (23%) and Sneathia (10%). The female microbiome was dominated by Prevotella bivia (28%), Escherichia coli (24%), Sneathia sanguinegens (7%) and Klebsiella pneumoniae (4%). On the other hand, the male microbiome was dominated by K. pneumoniae (23%) and E. coli (2%). K. pneumoniae and E. coli were the most abundant species found in both microbiomes. The 16S rRNA gene sequencing used in this study successfully uncovered the composition of the urogenital microbiome, which might not have been possible with conventional culture methods.
Kasus Malaria di Puskesmas Amban Manokwari Papua Barat Nurlaili Farida Muhajir; Fitri Nadifah; Trisno Agung Wibowo; Yola Ramadhani
Jurnal Ilmiah Permas: Jurnal Ilmiah STIKES Kendal Vol 12 No 3 (2022): Jurnal Ilmiah Permas: jurnal Ilmiah STIKES Kendal: Supp Juli 2022
Publisher : Sekolah Tinggi Ilmu Kesehatan Kendal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (266.35 KB)

Abstract

Penyakit malaria merupakan permasalahan global. Malaria yang ditularkan kepada manusia melalui gigitan nyamuk Anopheles betina, mengancam jiwa dan dapat menimbulkan kematian. Indonesia mempunyai beberapa wilayah endemis penyakit ini. Insidensi tertinggi ditemukan di Provinsi Papua dan Papua Barat, dengan API (annual parasite incidence) per 1000 penduduk pada tahun 2017 yaitu 59,00 dan 14,97. Untuk mengetahui jumlah persentase kasus malaria serta gambaran perilaku pencegahan terhadap gigitan nyamuk di desa endemik malaria yaitu kabupaten Amban, Manokwari, Papua Barat. Jenis penelitian ini adalah deskriptif. Subjek penelitian sejumlah 80 responden yang diduga terinfeksi malaria dan melakukan pemeriksaan darah di laboratorium Puskesmas Amban Kabupaten Manokwari Papua Barat. Pengambilan spesimen darah kapiler, pembuatan preparat sediaan malaria serta pewarnaan dengan pewarna Giemsa, dilakukan pada bulan Juni-Juli 2019 di Laboratorium Puskesmas Amban, sedangkan pembacaan preparat dilakukan di Laboratorium Klinik STIKES Guna Bangsa Yogyakarta. Analisis data disajikan dalam bentuk tabel dan narasi. Dari 80 preparat yang diperiksa, ditemukan sampel yang positif malaria sebanyak 42 subjek (52,5%) dan negatif malaria sebanyak 38 subjek (47,5%). Spesies penyebab malaria adalah Plasmodium falciparum sebanyak 41 subjek (97,6%) dan Plasmodium vivax sebanyak 1 subjek (2,4%). Perilaku pencegahan dari gigitan nyamuk subjek terinfeksi malaria menunjukkan bahwa 39 subjek (48,7%) tidak menggunakan repelan; 25 subjek (31,3%) melakukan aktivitas di luar rumah pada malam hari. Persentase tertinggi kasus malaria di Puskesmas Amban disebabkan oleh spesies Plasmodium falcifarum. Faktor perilaku pencegahan gigitan nyamuk yang diduga berperan dalam terjadinya infeksi adalah tidak menggunakan repelan dan melakukan aktivitas di luar rumah pada malam hari.
Potensi Ekstrak Serai Wangi (Cymbopogon nardus (L.) Rendl.) sebagai Larvasida Nyamuk Aedes aegypti Fitri Nadifah; Desto Arisandi; Siti Mahmuda
Seminar Nasional Penelitian dan Pengabdian Kepada Masyarakat 2022: Prosiding Seminar Nasional Penelitian dan Pengabdian Kepada Masyarakat (SNPPKM 2022)
Publisher : Universitas Harapan Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.455 KB) | DOI: 10.35960/snppkm.v2i1.1170

Abstract

Demam Berdarah Dengue (DBD) merupakan salah satu permasalahan kesehatan masyarakat utama di seluruh daerah tropis, termasuk Indonesia. Nyamuk Aedes aegypti merupakan perantara utama penularan penyakit DBD. Salah satu cara untuk mengendalikan jumLah nyamuk adalah menggunakan larvasida. Serai wangi (Cymbopogon nardus (L.) Rendle) merupakan tanaman yang tersebar di banyak wilayah di Indonesia dan berpotensi sebagai larvasida alami karena mengandung sejumLah zat aktif, terutama sitronela dan geraniol. Tujuan penelitian ini adalah untuk mengetahui kematian larva nyamuk Ae. aegypti dengan perlakuan berbagai konsentrasi ekstrak batang serai wangi. Penelitian ini bersifat deskriptif menggunakan ekstrak serai wangi dengan konsentrasi 4%, 8%, 12%, 16% dan 20%. Sebagai kontrol negatif, digunakan air keran. Dua puluh larva nyamuk Ae. aegypti digunakan sebagai subyek penelitian pada masing-masing perlakuan. Pengamatan kematian larva dilakukan setiap 30 menit sekali selama 2 jam. Kontrol negatif dan konsentrasi terendah ekstrak tidak mengakibatkan kematian larva (0%), sedangkan konsentrasi 8% menghasilkan 40% kematian larva. Pada konsentrasi ekstrak 12%, 16% dan 100%, seluruh larva mati setelah perlakuan 2 jam. Konsentrasi ekstrak minimum yang efektif dapat mengakibatkan 50% kematian larva adalah 12%. Dari penelitian ini dapat disimpulkan bahwa ekstrak batang serai wangi memiliki potensi sebagai larvasida dan menggantikan larvasida konvensional dengan mengikuti standar etika yang telah ditetapkan.
Daya Hambat Minyak Atsiri Rimpang Kunyit terhadap Pertumbuhan Candida Albicans In Vitro Fitri Nadifah; Nurlaili Farida Muhajir; Fitri Retnoningsih
Jurnal Vokasi Kesehatan Vol 4, No 1 (2018): Januari 2018
Publisher : Poltekkes Kemenkes Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (237.5 KB) | DOI: 10.30602/jvk.v4i1.124

Abstract

Abstract: Inhibition Activity Of Turmeric Essential Oil Against The Growth Of Candida Albicans. Turmeric has rhizomes which contain essential oils and curcuminoid that has anti-fungal activity. The purpose of this research was to know the influence of essential oil of turmeric Rhizome (Curcuma domestica Val.) against the growth of Candida albicans in vitro. This was an experimental research with diffusion method. Essential oil obtained through distillation methods and the concentration used was 20%, 40%, 60%, 80% and 100%. The treatment was repeated three times. Anova test was used. Inhibition zone is formed at a concentration of 20%, 40%, 60%, 80%, 100% were 6,16 mm, 6,50 mm, 6,66 mm, 7,00 mm and 7.83 mm, respectively Results in analysis by one way ANOVA showed there was a significant difference in the variance of essential oil concentration against the inhibition zone (p< 0.05).Abstrak: Daya Hambat Minyak Atsiri Rimpang Kunyit Terhadap Pertumbuhan Candida Albicans In Vitro. Kunyit merupakan  salah satu rimpang yang diketahui memiliki kandungan minyak atsiri serta curcuminoid yang bersifat anti jamur. Tujuan penelitian ini untuk mengetahui pengaruh minyak atsiri rimpang kunyit (Curcuma domestica Val.) terhadap pertumbuhan Candida albicans secara in vitro. Jenis penelitian ini adalah penelitian eksperimental dengan metode difusi cara sumuran. Minyak atsiri didapatkan melalui   metode destilasi dan konsentrasi  yang digunakan adalah 20%, 40%, 60%, 80% dan 100%. Perlakuan diulang sebanyak tiga kali. Hasil penelitian di analisis dengan Anova. Hasil zona hambat yang terbentuk pada konsentrasi minyak atsiri 20%, 40%, 60%, 80%, 100% berturut-turut adalah 6,16 mm, 6,50 mm, 6,66 mm, 7,00 mm,  dan 7,83 mm. Hasil analisis dengan one way anova menunjukkan terdapat perbedaan yang signfikan pada berbagai konsentrasi minyak atsiri terhadap zona hambat yang dihasilkan (p<0,05). Dari penelitian ini dapat disimpulkan bahwa berbagai konsentrasi minyak atsiri rimpang kunyit dapat menghambat pertumbuhan Candida albicans. Kemampuan ini berpotensi dikembangkan lebih lanjut sebagai alternatif dalam pengobatan terhadap penyakit karena infeksi Candida albicans.
Co-Authors Anggraeni Anwarudin, Anwarudin Arum Nuryati Bayu Nur Khotib, Bayu Nur Bolosan, Fabio Andre Budi Setiadi Daryono BUDI SETIADI DARYONO Budi Setiadi Daryono Bulu, Martinus Tagu Clara Pattirane, Evelin Dani Setiawan Dapa Ole, Ericson Umbu Robaka De Jesus, Maria Carolina Desto Arisandi Dewi Rahadian, Titah Diah Irawati Dian Wuri Astuti Dian Wuri Astuti, Dian Dieta Dieon Saputri Endah Retnaningrum Endah Retnaningrum Endah Retnaningrum Famela Adelena, Fitria Farida Muhajir, Nurlaili Farida Muhajir, Nurlaili Fitri Nadifah Fitri Retnoningsih Fitriani, Audiah Fuadah Hidayat, Tsamrotul Ika Susilowati Indah Yuliana Barekola Lamablawa Indah Yuliana Barekola Lamablawa, Indah Yuliana Barekola Islami Burhanudin Khatrina, Syahdapuan Dara Lisa Susanti, Lisa Liza Novia Lani Maloko, Seprianto Maria D. Owa Lobo Maria Yasintha Bhoga Marthaning Tyas, Wina May Tri Mulyani Melani Lesmono, Inez Merry Elisabeth Mosa, Rambu Pedi Nindhea Ferliza, Yoan Nono, Arianto Nurlaili Farida Muhajir Nurlaili Farida Muhajir, Nurlaili Farida Ohoiwutun, Clara Shinta D.P Puspita, Elisabet Della Reni Aqwil Masithah Retnoningsih, Fitri Sanusi , Kristi Elisabeth Saraswati, Farikhatun Nisaa Sawitri, Nining Eka Siti Fatimah Siti Mahmuda Sofvirah R. Sohy Sofvirah R. Sohy, Sofvirah R. Sri Murtiningrum, Sri SRI RAHAYU Susliyanti, Maria Trisno Agung Wibowo Urfiyah Lisa Azizah, Urfiyah Lisa Velisshia, Velisshia Vixa Meindara Wayan Tunas Artama Wayan Tunas Artama Wayan Tunas Artama Yola Ramadhani Yuliana Prasetyaningsih Yuliana Prasetyaningsih Yuliana Prasetyaningsih Yuliana Prasetyaningsih Yuliana Prasetyaningsih