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All Journal PROSIDING SEMINAR NASIONAL Biogenesis: Jurnal Ilmiah Biologi Jurnal Kesehatan Masyarakat Andalas (Andalas Journal of Public Health) Prosiding Seminar Biologi Indonesian Journal of Biotechnology Jurnal Teknologi Laboratorium Journal of Health (JoH) Adi Widya : Jurnal Pengabdian Masyarakat Martabe : Jurnal Pengabdian Kepada Masyarakat ABDIMAS SILIWANGI Jurnal Biomedika Sang Pencerah: Jurnal Ilmiah Universitas Muhammadiyah Buton Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Jurnal Ilmiah Permas: Jurnal Ilmiah STIKES Kendal Jurnal Nasional Teknologi Terapan Nusantara Science and Technology Proceedings Jurnal Mitra Kesehatan Jurnal Biosense Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) J.Abdimas: Community Health SEMINAR NASIONAL PENELITIAN DAN PENGABDIAN KEPADA MASYARAKAT Jurnal Vokasi Kesehatan Gema Kesehatan Journal Fatmawati Laboratory & Medical Science

Fitri Nadifah

Program Studi D3 Analis Kesehatan STIKES Guna Bangsa Yogyakarta

Author-ID : 484168

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Pengaruh Infusa Daun Sambiloto (Andrographis paniculata Nees) Terhadap Pertumbuhan Bakteri Shigella dysenteriae Secara In Vitro Fitri Nadifah; Siti Fatimah; Indah Yuliana Barekola Lamablawa
Journal of Health (JoH) Vol 1 No 1 (2014): Journal of Health - January 2014
Publisher : LPPM STIKES Guna Bangsa

Background: Bacteria Shigella dysenteriae is pathogenic bacterium which cause diarrheal diseases that enter into inside the body through intestine. Shigella dysenteriae constitute one of the gram negative bacterium which causes inflammation on intestinal, pain abdominal, tenesmus, and a bowel movements which accompanied with blood and mucus. Bad impact irational use of synthethic drugs and antibiotics can cause organ damage and germs resistance. Utilization of herbs as traditional medicine evolving rapidly and alternatively used by most. Traditional medicine has relatively low of side effect and affordable prices. Sambiloto (Andrographis paniculata Nees) is a plant which is ordinary used as a traditional medicine that can easily found around Indonesian islands. Sambiloto constitute one of the medicinal plants which is allegedly has the antibacterial effect because its chemical content of flavonoid saponins and tannin. The objective of this study is to know the influence of sambiloto (Andrographis paniculata Nees) leaves infuse against the growth of Shigella dysenteriae bacteria. Method: This is an experimental research using diffusion method. Statistical test who used namely analysis of variant one road (One Way anova). Analysis data were done descriptively and statistically and presented with of tables or graphs. Results: There is influence of sambiloto leaf infusa (Andrographis paniculata Nees) against the growth of Shigella dysenteriae bacteria in vitro with a mean diameter zone of radical on concentrations the smallest 20% is 12 mm and the greatest concentration 100% is 24.3 mm. Conclusion: Infuse leaf has a strong and positive influence in inhibit the growth of bacteria Shigella dysenteriae bacterium.

Pengaruh Lama Penyimpanan Susu Kedelai dalam Lemari Es Terhadap Pertumbuhan Bakteri Psikrofilik Bayu Nur Khotib; Yuliana Prasetyaningsih; Fitri Nadifah
Journal of Health (JoH) Vol 2 No 1 (2015): Journal of Health - January 2015
Publisher : LPPM STIKES Guna Bangsa

Background: Soy milk is a drink with full of nutrients, especially contains high protein and vitamin. In room’s temperature, soy milk easy to be damage because of bacterial’s activity. Storaging in low temperature is one of control effort to prevent damaging in soy milk. Research Purpose: To find out whether there is influence of soy milk long-time storaging in refrigator toward bacterial psychrophilic’s growth. Research methods: Methods which use in this research is quasi experimental with pre and post test with control design program. Intervention to sample is storaging the sample in refrigator during 1 until 5 days. Results: Data results obtained from this research was the highest number of colony’s growth occured in days 3 with 4733,33 CFU/ml and that number not exceed yet the bacterial pollution number based on soy milk SNI that is 1 x 106. Conclusion: There was influence of soy milk long-time storaging in refrigator toward bacterial psychrophilic’s growth.

Pengaruh Infusa Daun Jambu Biji (Psidium guajava Linn.) Terhadap Pertumbuhan Bakteri Escherichia coli Secara In Vitro Fitri Nadifah; Siti Fatimah; Lisa Susanti
Journal of Health (JoH) Vol 2 No 2 (2015): Journal of Health - July 2015
Publisher : LPPM STIKES Guna Bangsa

Background: Diarrhea is still a major health problem in Indonesia. This is due to the lack of knowledge about society hygiene and sanitation as well as there are still many people who haven't got health access. Guava (Psidium guajava) is a plant that has long been known by locals as traditional medicine. Some research has shown that extracts of the guava flesh is effective as an antidiarrheal. In addition, guava possessed efficacy as an anti-inflammatory, anti-mutagenic, anti-microbial and analgesic. Objective: To determine the effect of various concentrations of guava infusion leaves inhibiting E. coli growth in vitro. Methods: Third, fourth and fifth leaf from the tip were used as the materials. Leaves infusion concentration used was 20%, 40%, 60%, 80% and 100%. Each concentration infusion loaded into wells of Mueller-Hinton (MH) agar media. Radical zone around the wells measured as bacterial growth inhibition. The data were analyzed using one way analysis of variance (One-way ANOVAs). Results: There is the influence of infusion of guava leaves (Psidium guajava) on the growth of E. coli in vitro with a mean diameter of the smallest concentration of radicals zone which is 20% of 15.3 mm and the greatest concentration of 100% by 22 mm. Conclusion: Infusion of guava leaves have a strong influence in inhibiting E. coli growth as it has antibacterial chemical components.

Helminth Infection of Children in Ngemplak Seneng Village Klaten Fitri Nadifah; Desto Arisandi; Nurlaili Farida Muhajir
Journal of Health (JoH) Vol 3 No 1 (2016): Journal of Health - January 2016
Publisher : LPPM STIKES Guna Bangsa

Helminth infection remains one of health problems of Indonesian people for years. The tropical condition of Indonesian climates with high level humidity has believed to be the one of factors that increase the number of this infection cases. Based of many studies, the number of helminth infections is related to the poor of hygiene and sanitation. This study is conducted to know the percentages of helminth infections among 0-14 years old children in Dusun Ngemplak, Desa Ngemplak Seneng, Klaten and also their personal hygiene with questionaire instruments. To know helminth infection in subjects, we examine faeces sample with simple method and 1% eosin dye. As the results of examination, we found Trichuris trichiura egg in three samples. This might related to the poor of their hygienic behaviors. Some of the behavior such as wash hand before eating and after defecation, use sandal or shoes when playing, consume helminthic drug for last 6 months, and defecate in toilet has shown percentage under 70%. Other behaviors such as cutting and cleaning nails and use a spoon when eating has shown percentage above 70%.

Identification of Malaria Parasites in Chasan Boesoirie General Hospital Ternate East Nusa Tenggara Desto Arisandi; Sofvirah R. Sohy; Fitri Nadifah
Journal of Health (JoH) Vol 3 No 1 (2016): Journal of Health - January 2016
Publisher : LPPM STIKES Guna Bangsa

Background: Malaria is still a global health problem especially in tropical countries, such as Indonesia. Based on survey 35% of the Indonesia population were live in endemic areas of malaria, such as Provinces of Maluku, North Maluku, Papua, West Papua, North Sumatra, and East Nusa Tenggara. Malaria is caused by a mosquito bite of female Anopheles sp. containing Plasmodium parasite. Generally it bites humans at night or dawn. Such malaria parasites are Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, and Plasmodium ovale. Malaria is a disease of both highest in Ternate and mostly occur in general hospitals of Chasan Boesoirie General Hospital. This research aims to identify the type of malaria parasites in the Chasan Boesoirie General Hospital Ternate. Method: This is a descriptive research conducted in Chasan Boesoirie General Hospital in Ternate, East Nusa Tenggara in March 2015. Research data obtained include: age, sex, education, and employment. Identification of malaria is carried out based on an examination of the existence of Plasmodium in preparation of thin and thick blood apus. Descriptive analysis using STATA programs for Windows version 12.0 (Stata Corp LP., College Station, TX, USA) are presented in the form of tables and narratives. Result: This research subjects were 69 people suspected with malaria infection. Suspected malaria to be infected by Plasmodium is more are young age group 94%, 88% were males, 100% have a poor education or un-educated, as well as the of 100% were fishermen. As much as 54 people (78%) were infected by Plasmodium parasites consisting of Plasmodium falcifarum 48 people (89%) and Plasmodium vivax 6 people (11%). Conclusion: The incidence of malaria infections in Chasan Boesoirie General Hospital amounted to 78% and the type of infected parasites were Plasmodium falcifarum and Plasmodium vivax.

Efektivitas Infusa Daun Talok (Muntingia calabura) dalam Menghambat Pertumbuhan Bakteri Klebsiella pneumoniae Fitri Nadifah; Desto Arisandi; Siti Fatimah; Dani Setiawan
Jurnal Nasional Teknologi Terapan (JNTT) Vol 2, No 3 (2018): NOVEMBER
Publisher : Penelitian dan Pengabdian Kepada Masyarakat Sekolah Vokasi Universitas Gadjah Mada

Klebsiella pneumoniae is one of the most bacteria found in urine of Urinary Tract Infection (UTI) Patients. Study in many countries has found that this species become resistant to more than one antibiotics. This research goal is to know the effectiveness of talok (Muntingia calabura) infused water inhibiting the growth of K. pneumoniae. This was an experimental study using K. pneumoniae bacteria isolated from urine samples using diffuse agar method. The concentration of talok infused water was varied from 25-100% and the antibiotics used was cefotaxime, meropenem and chloramphenicol. The inhibition activity was measured by the diameter of inhibition zone. The results show that K. pneumoniae is still give sensitive response to antibiotic treatments. While the inhibition zone from talok infused water treatments can be categorized in middle and strong activity. From this study we can conclude that talok infused water potential in inhibiting the growth of K. pneumoniae. Future study still needed to develop this potential, especially for the bacteria that has been resistant to some antibiotics.

Penyuluhan Perilaku Hidup Bersih Dan Sehat (PHBS) Di Dusun Berjo Wetan Godean Sleman Yogyakarta Dian Wuri Astuti; Fitri Nadifah; Yuliana Prasetyaningsih; Vixa Meindara; Anggraeni; Diah Irawati; May Tri Mulyani
J.Abdimas: Community Health Vol 1 No 1 (2020): J.Abdimas: Community Health - November 2020
Publisher : LPPM STIKES Guna Bangsa Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30590/jach.v1i1.232

Clean and healthy living behavior (PHBS) is a step that must be taken to achieve an optimal health degree for everyone. Cultivating a healthy lifestyle in society must be initiated and worked on from oneself. Meanwhile, public awareness of health and hygiene and healthy lifestyles, especially rural communities is still very low. For this reason, presenting counseling is expected to increase public knowledge and awareness of the importance of making clean and healthy living behaviors (PHBS) in daily life so that a better health degree can be realized.

Lactobacillus Composed Urinary Microbiome of Healthy People and Urinary Tract Infection Patients Fitri Nadifah; Wayan Tunas Artama; Budi Setiadi Daryono; Endah Retnaningrum
Sang Pencerah: Jurnal Ilmiah Universitas Muhammadiyah Buton Vol 8 No 4 (2022): Sang Pencerah: Jurnal Ilmiah Universitas Muhammadiyah Buton
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Universitas Muhammadiyah Buton

Lactobacillus has long been known to reconstruct the microbiome of the urogenital organs of healthy people. However, changes in lactobacillus abundance are known occurring in urogenital tract infections (UTI) patients. It is also known that there are Lactobacillus species that have specific genomic characteristics and nutritional requirements that cause them to turn into pathogens. Understanding population characteristics and taxa composition that reconstruct urine microbiome is very important for the diagnosis and treatment of urogenital infections. This review provides an update information of metagenomic sequencing to identify the urinary microbiome profile and the role of lactobacilli in healthy and UTI patients.

Characterization of the urogenital microbiome in patients with urinary tract infections Fitri Nadifah; Wayan Tunas Artama; Budi Setiadi Daryono; Endah Retnaningrum
Indonesian Journal of Biotechnology Vol 27, No 3 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.69212

Standard microbiological culture techniques can only identify a fraction of the urogenital microbiome. Meanwhile, identifying and characterizing infectious microorganisms are very important for the success of diagnosis and treatments, especially for Urinary Tract Infection (UTI) patients. This study aimed to characterize the urogenital microbiome of UTI patients using 16S rRNA gene sequencing. We sequenced two pooled DNA samples from voided urine of UTI patients (21 females and 13 males). To determine the structure and composition of taxa in the samples, 16S rRNA gene sequencing was performed using the Illumina Mi‐Seq paired‐end platform. The most abundant genera were Burkholderia‐Caballeronia‐Paraburkholderia (71%) followed by Prevotella (33%), Escherichia‐Shigella (24%), Klebsiella (23%) and Sneathia (10%). The female microbiome was dominated by Prevotella bivia (28%), Escherichia coli (24%), Sneathia sanguinegens (7%) and Klebsiella pneumoniae (4%). On the other hand, the male microbiome was dominated by K. pneumoniae (23%) and E. coli (2%). K. pneumoniae and E. coli were the most abundant species found in both microbiomes. The 16S rRNA gene sequencing used in this study successfully uncovered the composition of the urogenital microbiome, which might not have been possible with conventional culture methods.

Kasus Malaria di Puskesmas Amban Manokwari Papua Barat Nurlaili Farida Muhajir; Fitri Nadifah; Trisno Agung Wibowo; Yola Ramadhani
Jurnal Ilmiah Permas: Jurnal Ilmiah STIKES Kendal Vol 12 No 3 (2022): Jurnal Ilmiah Permas: jurnal Ilmiah STIKES Kendal: Supp Juli 2022
Publisher : Sekolah Tinggi Ilmu Kesehatan Kendal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (266.35 KB)

Penyakit malaria merupakan permasalahan global. Malaria yang ditularkan kepada manusia melalui gigitan nyamuk Anopheles betina, mengancam jiwa dan dapat menimbulkan kematian. Indonesia mempunyai beberapa wilayah endemis penyakit ini. Insidensi tertinggi ditemukan di Provinsi Papua dan Papua Barat, dengan API (annual parasite incidence) per 1000 penduduk pada tahun 2017 yaitu 59,00 dan 14,97. Untuk mengetahui jumlah persentase kasus malaria serta gambaran perilaku pencegahan terhadap gigitan nyamuk di desa endemik malaria yaitu kabupaten Amban, Manokwari, Papua Barat. Jenis penelitian ini adalah deskriptif. Subjek penelitian sejumlah 80 responden yang diduga terinfeksi malaria dan melakukan pemeriksaan darah di laboratorium Puskesmas Amban Kabupaten Manokwari Papua Barat. Pengambilan spesimen darah kapiler, pembuatan preparat sediaan malaria serta pewarnaan dengan pewarna Giemsa, dilakukan pada bulan Juni-Juli 2019 di Laboratorium Puskesmas Amban, sedangkan pembacaan preparat dilakukan di Laboratorium Klinik STIKES Guna Bangsa Yogyakarta. Analisis data disajikan dalam bentuk tabel dan narasi. Dari 80 preparat yang diperiksa, ditemukan sampel yang positif malaria sebanyak 42 subjek (52,5%) dan negatif malaria sebanyak 38 subjek (47,5%). Spesies penyebab malaria adalah Plasmodium falciparum sebanyak 41 subjek (97,6%) dan Plasmodium vivax sebanyak 1 subjek (2,4%). Perilaku pencegahan dari gigitan nyamuk subjek terinfeksi malaria menunjukkan bahwa 39 subjek (48,7%) tidak menggunakan repelan; 25 subjek (31,3%) melakukan aktivitas di luar rumah pada malam hari. Persentase tertinggi kasus malaria di Puskesmas Amban disebabkan oleh spesies Plasmodium falcifarum. Faktor perilaku pencegahan gigitan nyamuk yang diduga berperan dalam terjadinya infeksi adalah tidak menggunakan repelan dan melakukan aktivitas di luar rumah pada malam hari.

Co-Authors Anggraeni Anwarudin, Anwarudin Arum Nuryati Bayu Nur Khotib Bayu Nur Khotib, Bayu Nur Bolosan, Fabio Andre Budi Setiadi Daryono Budi Setiadi Daryono BUDI SETIADI DARYONO Bulu, Martinus Tagu Clara Pattirane, Evelin Dani Setiawan Dapa Ole, Ericson Umbu Robaka De Jesus, Maria Carolina Desto Arisandi Dewi Rahadian, Titah Diah Irawati Dian Wuri Astuti Dian Wuri Astuti, Dian Dieta Dieon Saputri Endah Retnaningrum Endah Retnaningrum Endah Retnaningrum Famela Adelena, Fitria Farida Muhajir, Nurlaili Farida Muhajir, Nurlaili Fitri Nadifah Fitri Retnoningsih Fitriani, Audiah Fuadah Hidayat, Tsamrotul Ika Susilowati Indah Yuliana Barekola Lamablawa Indah Yuliana Barekola Lamablawa, Indah Yuliana Barekola Islami Burhanudin Khatrina, Syahdapuan Dara Lisa Susanti Lisa Susanti, Lisa Liza Novia Lani Maloko, Seprianto Maria D. Owa Lobo Maria Yasintha Bhoga Marthaning Tyas, Wina May Tri Mulyani Melani Lesmono, Inez Merry Elisabeth Mosa, Rambu Pedi Nindhea Ferliza, Yoan Nono, Arianto Nurlaili Farida Muhajir Nurlaili Farida Muhajir, Nurlaili Farida Ohoiwutun, Clara Shinta D.P Puspita, Elisabet Della Reni Aqwil Masithah Retnoningsih, Fitri Sanusi , Kristi Elisabeth Saraswati, Farikhatun Nisaa Sawitri, Nining Eka Siti Fatimah Siti Mahmuda Sofvirah R. Sohy Sofvirah R. Sohy, Sofvirah R. Sri Murtiningrum, Sri SRI RAHAYU Susliyanti, Maria Trisno Agung Wibowo Urfiyah Lisa Azizah, Urfiyah Lisa Velisshia, Velisshia Vixa Meindara Wayan Tunas Artama Wayan Tunas Artama Wayan Tunas Artama Yola Ramadhani Yuliana Prasetyaningsih Yuliana Prasetyaningsih Yuliana Prasetyaningsih Yuliana Prasetyaningsih Yuliana Prasetyaningsih Yuliana Prasetyaningsih

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