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All Journal Natural Science: Journal of Science and Technology Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Transformasi: Jurnal Pengabdian Masyarakat KOVALEN: Jurnal Riset Kimia Jurnal Teknologi Agro-Industri Indonesian Journal of Chemistry AR-RAZI Jurnal Ilmiah Fullerene Journal of Chemistry Jurnal Ilmiah Teknik Kimia Indonesian Journal of Chemical Science and Technology
Jaya Hardi
Chemistry Department, Science And Mathematic Faculty Of Tadulako University, Bumi Tadulako Tondo Campus, Palu Sulawesi Tengah 94111
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PENGGUNAAN PROTEASE DARI GETAH BIDURI DALAM PRODUKSI FLAVOR UDANG WINDU (PENAEUS MONODON) Hardi, Jaya; Diharnaini, Diharnaini
Natural Science: Journal of Science and Technology Vol 3, No 2 (2014): Volume 3 Number 2 (August 2014)
Publisher : Univ. Tadulako

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Abstract

Shrimp flavor including one of the high-value product that can be produced from the sap biduri protease activity. This research intends to know the concentration of biduri protease isolated and reaction times that result high-quality shrimp flavor. Achievement of objectives has been applied randomized block design with factorial treatment pattern that consists of two factors, namely the biduri protease concentration factor with three levels and hydrolysis time factor with three levels. Observed parameters include water content, soluble protein content and organoleptic quality of shrimp flavor. The results obtained indicate the concentration of biduri protease having an affect on the water content, soluble protein content and organoleptic quality, while the hydrolysis time having an affect only on the soluble protein content and organoleptic quality. The highest soluble protein content (71.65%) and the lowest water content (11.08%) was found in the combination of biduri protease concentration of 2.5% for 2 hours hydrolysis.
OPTIMALISASI PENERAPAN BIOTEKNOLOGI DALAM PRODUKSI BIOETANOL DARI SAGU (METROXYLON SP.) Widayanti, Sayu Nila; Mappiratu, Mappiratu; Hardi, Jaya
Natural Science: Journal of Science and Technology Vol 5, No 1 (2016): Volume 5 Number 1 (March 2016)
Publisher : Univ. Tadulako

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Abstract

"> Telah dilakukan penelitian tentang optimalisasi penerapan bioteknologi dalamproduksi bioetanol dari sagu. Optimalisasi mencangkup pengaruh konsentrasi enzim ?-amilase dan waktu hidrolisis terhadap glukosa yang dihasilkan dari hidrolisis pati sagu sertapengaruh waktu fermentasi terhadap kadar bioetanol yang dihasilkan. Penelitian ini dirancang menggunakan Rancangan Acak Lengkap (RAL) dengan tiga taraf ulangan. Variasi konsentrasi enzim ?-amilase dirancang dengan 5 taraf (0; 0,05; 0,06; 0,07; 0,08% (v/v)) dan waktu hidrolisis (1; 1,5; 2; 2,5; 3 jam) dimana kadar glukosa yang dihasilkan dianalisis menggunakan metode DNS. Hasil penelitian menunjukkan konsentrasi enzim 0,08% (v/v) dengan waktu hidrolisis tetap (1 jam) menghasilkan kadar glukosa tertinggi yaitu sebesar 9,25% dan waktu hidrolisis 3 jam dengan konsentrasi enzim tetap (0,08% (v/v)) menghasilkan kadar glukosa tertinggi yaitu sebesar 11,41%. Variasi waktu fermentasi dengan 5 taraf (3; 4; 5; 6; 7 hari) dimana bioetanol yang dihasilkan dimurnikan dengan metode destilasi dan diukur dengan alkohol meter. Hasil penelitian menunjukkan waktu fermentasi tertinggi diperoleh pada hari ke-7 dengan kadar bioetanol 5,75%.
SIFAT FISIKO-KIMIA EDIBLE FILM AGAR – AGAR RUMPUT LAUT (GRACILARIA SP.) TERSUBTITUSI GLYSEROL Setyaningrum, Annisa; Sumarni, Ni Ketut; Hardi, Jaya
Natural Science: Journal of Science and Technology Vol 6, No 2 (2017): Volume 6 Number 2 (August 2017)
Publisher : Univ. Tadulako

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Abstract

Telah dilakukan penelitian tentang pengaruh penambahan gliserol pada pembuatanedible film dari agar ? agar rumput laut (gracilaria sp.). Tujuan penelitian ini adalah untuk mengetahui sifat fisiko-kimia terbaikedible film berbasis agar dengan plasticizer gliserol. Penelitian ini menerapkan variasi konsentrasi gliserol antara lain 30, 35, 40, 45, dan 50%terhadap massa agar-agar atas dasar (b/b). Sifat fisiko-kimia Edible filmyang diuji antara lain ketebalan menggunakan micrometer, laju transmisi uap air, kuat tarik dan elongasi masing-masing diuji dengan Universal Testing Machine. Konsentrasi gliserol terbaik diperoleh pada penambahan gliserol 45% yang memiliki ketebalan 0,0167 mm, laju transmisi uap air sebesar 57,18 g/24jam.m2, kuat tarik sebesar 14,23 MPa, dan elongasi sebesar 12,75%
SINTESIS O-KARBOKSIMETIL KITOSAN PADA BERBAGAI KONSENTRASI NaOH DAN SUHU REAKSI SERTA APLIKASINYA SEBAGAI ANTIBAKTERI Jaya Hardi; Nurakhirawati Nurakhirawati; Ahmad Ridhay; Musdalifah Musdalifah
KOVALEN: Jurnal Riset Kimia Vol. 3 No. 1 (2017): Edisi April
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

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Abstract

The research about the influence of NaOH concentrations and reaction temperature to O-Carboxymethyl Chitosan (O-CMC) synthesis and it is antibacterial application was done. This research intent on detect NaOH concentration and reaction temperature that resulting of O-CMC with highest of yield, solubility, and substitution degrees, and also to find out FTIR spectrum and antibacterial activity of O-CMC. The produce of O-CMC was done by using a Completely Randomized Design (CRD) with the variation of NaOH concentration of 20%, 30%, 40%, 50%, and 60%, and also reaction temperature of 20, 30, and 40oC. The result was showed that on the NaOH concentration of 60% (w/v) and temperature of 30oC was resulted highest of yield, solubility, and substitution degrees of 41.5%, 31 g/L, and 0.67, respectively. FTIR spectrum was showed that bending vibration of primary amine (-NH2) give two bands for O-CMC. The broadest inhibition area of O-CMC (1.43 cm) was showed on Salmonella sp.Keywords: chitosan, O-CMC, substitution degree, antibacterial
KARAKTERISASI ENZIM KITINASE DARI ISOLAT BAKTERI TERMOFILIK B1211 ASAL AIR PANAS BORA Jaya Hardi; Ruslan Ruslan; Abd. Rahman Razak; Silva Silva
KOVALEN: Jurnal Riset Kimia Vol. 3 No. 2 (2017): Edisi Agustus
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

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Abstract

Penelitian ini bertujuan untuk mengkarakterisasi enzim kitinase dari bakteri termofilik asal sumber air panas Bora. Isolat bakteri B1211 digunakan pada produksi enzim kitinase pada media MSM cair yang diperkaya dengan konsentrasi koloidal kitin 1% pada suhu inkubasi 60oC selama 3 hari. Aktivitas enzim kitinase dianalisis berdasarkan standar N-asetil-D-glukosamin untuk kondisi suhu dan pH optimum, pengaruh substrat, dan pengaruh ion logam. Hasil penelitian menunjukkan enzim kitinase memiliki suhu optimum, pH optimum, dan konsentrasi substrat terbaik, masing-masing 600C, pH 7, dan 2%. Kinetika enzim kitinase memiliki Vmaks dan Km, yaitu 0,72 U/mL dan 0,01 mg/mL. Ion logam Hg2+, Zn2+, Al3+, dan Cu2+ berfungsi sebagai activator, sedangkan ion Li3+, Ca2+, dan K+ cenderung sebagai inhibitor terhadap enzim kitinase.Kata kunci: bakteri termofilik, isolat B1211, kitinase, karakterisasi
PEMANFAATAN KULIT UBI KAYU SEBAGAI BIOSORBEN PADA PENJERNIHAN AIR DI KELURAHAN BALAROA Syaiful Bahri; Musafira Musafira; Jaya Hardi; Pasjan Satrimafitrah
KOVALEN: Jurnal Riset Kimia Vol. 3 No. 2 (2017): Edisi Agustus
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

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Abstract

Kajian mengenai penjerapan ion logam Fe dalam air tanah menggunakan biosorben kulit ubi kayu telah berhasil dilakukan. Tujuan dari kajian ini adalah untuk menentukan pengaruh biosorben kulit ubi kayu terhadap sejumlah ion Fe dengan modifikasi perangkat alat penjernihan. Air tanah diperoleh dari Kelurahan Balaroa, Kota Palu, Indonesia. Biosorben kulit ubi kayu dikombinasikan dengan arang dan kerikil dalam perangkat alat penjernihan. Hasil kajian menunjukkan bahwa air tanah hasil penyaringan lebih jernih dibandingkan sebelum perlakuan. Air tanah hasil penyaringan tidak menghasilkan endapan besi setelah penyimpanan selama dua minggu. Kata kunci: ion Fe, biosorben, kulit ubi kayu, air tanah
SINTESIS HIDROKSIAPATIT DARI TULANG SAPI DENGAN METODE BASAH PADA BERBAGAI WAKTU PENGADUKAN DAN SUHU SINTERING Reflin Yuliana; Erwin Abdul Rahim; Jaya Hardi
KOVALEN: Jurnal Riset Kimia Vol. 3 No. 3 (2017): Edisi Desember
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

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Abstract

Synthesis of hydroxyapatite (HAp) can be obtained from a reaction between calcium of cow bones and phosphate under wet method. Completely Random Design (CRD) was used in this research with three levels of stirring time and four levels of sintering temperature i.e. 60, 90, 120 minutes and 800oC, 850oC, 900oC, 950oC, respectively. The result showed that synthesis reaction at the sintering temperature of 850oC for 120 minutes were the best conditions to produce HAp. The yield of HAp product in those conditions was 48,5%. XRD spectrum of HAp had the highest intensity of 1000 at a 2Ɵ angel of 31.78o.Keywords: Hydroxyapatite, Stirring Time, Sintering Temperature, Cow Bone
Mikroenkapsulasi Ekstrak Kulit Buah Naga Super Merah (Hylocereus costaricensis) pada Berbagai Massa Maltodekstrin dan Aplikasinya Sebagai Antioksidan: Microencapsulation of Super Red Dragon Fruit (Hylocereus costaricensis) in Various of Maltodextrin Mass and its Application as an Antioxidant Sucianti; Nurhaeni; Jaya Hardi
KOVALEN: Jurnal Riset Kimia Vol. 6 No. 3 (2020): Edisi Desember
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/kovalen.2020.v6.i3.9889

Abstract

Red super dragon fruit peel (Hylocereus costaricensis) extract has capability to scavenge DPPH and the activity could be maintained by using maltodextrin as coating. The aims of the research are to determine ratio of red super dragon fruit peel ethanol extract toward maltodextrin producing microcapsule with the highest coating efficiency and DPPH scavenging content by microcapsule of red super dragon fruit peel extract. Microencapsulation was carried out using freeze drying technique. Comparison of red super dragon fruit peel extract and maltodextrin were 1: 8, 1: 10, 1: 12, and 1:14 (w / w). The results show that the highest efficiency ratio of coating extract of red super dragon fruit peel with maltodextrin was 1: 8 with percentage of 90,77%. Level of DPPH scavenging content of red super dragon fruit peel extract microcapsule with ratio of 1:8 on 5 minutes and 60 minutes each is 27.36% and 46.62%. Keywords: Hylocereus costaricensis, freeze drying, maltodextrin, microencapsulation
KAJIAN EKSTRAKSI GELATIN DARI TULANG IKAN MUJAIR (Oreochromis mossambicus) Darwin Darwin; Ahmad Ridhay; Jaya Hardi
KOVALEN: Jurnal Riset Kimia Vol. 4 No. 1 (2018): Edisi April
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (408.324 KB) | DOI: 10.22487/kovalen.2018.v4.i1.10177

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh waktu perendaman dan rasio antara berat tulang ikan mujair terhadap volume asam sitrat 9% (b/v) dalam menghasilkan gelatin dengan rendemen tertinggi dan mutu terbaik. Penelitian ini dilakukan menggunakan rancangan acak lengkap (RAL) dengan 5 taraf waktu kontak yaitu 24, 36, 48, 60,dan 72 jam dan 5 taraf rasio berat tulang ikan mujair: asam sitrat 1:2, 1:3, 1:4, 1:5 dan 1:6. Penentuan pengaruh waktu perendaman dan rasio dilakukan dengan menggunakan metode pembobotan berdasarkan hasil uji statistik dengan menggunakan 5 parameter pengujian yakni rendemen, kadar abu, kadar air, viskositas, dan kekuatan gel. Dari hasil penelitian menunjukkan bahwa pengaruh waktu perendaman dengan menggunakan 5 parameter tertinggi adalah 36 jam dan rasio adalah 1:6, dengan rendemen 9,439%, kadar abu 2,8%, kadar air 5,81%, viskositas 5,24 cP, dan kekuatan gel terbaik 38,1 mm/kg.sKata kunci : Gelatin, tulang ikan mujair, asam sitrat, viskositas, kekuatan gel
PENGGUNAAN KULIT UBI KAYU (Manihot utillisima Pohl) TERHADAP PENYERAPAN LOGAM Fe (III) PADA AIR PAYAU Delta Rinanda Duda; Syaiful Bahri; Jaya Hardi; Nov Irmawati Inda
KOVALEN: Jurnal Riset Kimia Vol. 5 No. 2 (2019): Edisi Agustus
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (380.243 KB) | DOI: 10.22487/kovalen.2019.v5.i2.11469

Abstract

The absorption of Fe (III) metal in brackish water in the Balaroa Village, West Palu District using Cassava peels (Manihot utillisima Pohl) has been carried out. The aim of this study is determining the lifetime of cassava peels (Manihot utillisima Pohl) on the absorption of Fe (III) ions in brackish water. The research was conducted in a simple purification water system. Brackish water was flowed through the simple purification water system containing Cassava peels (Manihot utillisima Pohl) with a volume of brackish water is 10 liters per day (measured flow rate) and been analyzed every 2 days using UV-Vis spectrophotometry for less than 1 month. The results of the study show that cassava peel could be used as biosorbent to absorb and reduce level of Fe (III) ions in brackish water for 2.821 months. The concentration of Fe (III) ions in brackish water in Balaroa Village, West Palu District is 1.666 ppm. Keywords : Fe (III) Ion, Biosorbent, Cassava Peel
Co-Authors Abd. Rahman Razak Ahmad Ridhay Ayu Satmah Bahri Syaiful Bryan Agung Rundubelo Darwin Darwin Delta Rinanda Duda Desi Hardianti Dian Citra Diharnaini Diharnaini Dini Noviyandari Dwi Juli Pusptasari Dwi Juli Pusptasari Erwin Abdul Rahim Gladys Hardi Ys Indriani Indriani Isabella de Nadra Khairunnisa Khairunnisa LAKSMI AMBARSARI Mappiratu Mappiratu Mappiratu Mappiratu Musafira Musafira Musdalifah Musdalifah Ni Ketut Sumarni Nov Irmawati Inda Novaldi Novaldi Nur A Atjiang Nurakhirawati Nurakhirawati Nurhaeni Nurhaeni Nurhaeni Nurhaeni Nurhaeni Nurhaeni Nurhaeni Nurhaeni Nurhaeni Pasjan Satrimafitrah Prismawiryanti Prismawiryanti Purwantiningsih Sugita Putri Hardiyanti Putri Wahyuni Reflin Yuliana Rian S Rauf Riza Aulia Putri Buheli Ruslan Lina Ruslan Ruslan Sayu Nila Widayanti, Sayu Nila Setyaningrum, Annisa Silva Silva Sucianti Syaiful Bahri Syaiful Bahri Syamsuddin Syamsuddin Windi Windi