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All Journal The Journal of Pure and Applied Chemistry Research Biocelebes KOVALEN: Jurnal Riset Kimia al Kimiya : Jurnal Ilmu Kimia dan Terapan
Pasjan Satrimafitrah
Department Of Chemistry, Faculty Of Mathematics And Science, Tadulako University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Materials Science & Nanotechnology Medicine & Pharmacology

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Potensi Ekstrak Batang Kayu Gempol (Nauclea orientalis L.) dalam Menghambat Pertumbuhan Bakteri: Potency of Gempol (Nauclea orientalis L.) Stem Extracts to Inhibiting Bacterial Growth Ni Kadek Atmiyanti; Pasjan Satrimafitrah; Abd. Rahman Razak; Nov Irmawati Inda; Indriani; Dwi Juli Puspitasari
KOVALEN: Jurnal Riset Kimia Vol. 8 No. 2 (2022): August Edition
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/kovalen.2022.v8.i2.15962

Abstract

The study of the potential of Gempol wood stem extract (Nauclea orientalis L.) with three solvent polarities has been exercised to determine the extract with a certain polarity and the smallest concentration that can inhibit the growth of bacteria Staphylococcus aureus and Escherichia coil. Extraction is performed using the maceration method with n-hexane, ethyl acetate, and ethanol as solvents. Furthermore, the potency in inhibiting the growth of bacteria was observed by the diffusion method with a range of extract concentrations from the largest to the smallest. The extract's ability to inhibit bacterial growth in the time range of up to 24 hours was measured using the turbidimetric method. The results showed that ethyl acetate and ethanol extracts were able to inhibit the growth of both bacteria in a wide concentration range (100 %– 5%), although n-hexane extracts showed the highest percentage in inhibiting both bacteria (15.27 mm and 16.89 mm, respectively). Turbidimetric assays showed the extract was stronger in inhibiting S. aureus compared to E. coli in the logarithmic phase range of 12 hours. This study showed that semipolar and polar extracts can inhibit the growth of bacteria with a wider concentration range compared to non-polar extracts despite having a higher inhibition capability.
Produksi Konsentrat Protein dari Ampas Kelapa (Cocos nucifera L.) Menggunakan NaOH dan (NH4)2SO4 : Production of Protein Concentrate from Coconut Pulp (Cocos nucifera L.) using NaOH and (NH4)2SO4 Hasdinda Hasdinda; Pasjan Satrimafitrah; Syaiful Bahri; Abd. Rahman Razak; Dwi Juli Puspitasari; Indriani; Aini Auliana Amar
KOVALEN: Jurnal Riset Kimia Vol. 9 No. 1 (2023): April Edition
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/kovalen.2023.v9.i1.16290

Abstract

Coconut pulp wasted from processed coconut oil has a protein content of up to 18.20%. The potential coconut pulp protein can be processed in protein concentrate which has many benefits. This research was conducted to determine the effect of NaOH and (NH4)2SO4 concentrations in isolating protein and producing protein concentrates that have the highest yield and protein content. Isolation of coconut pulp protein was carried out using NaOH at several concentrations, namely 0, 0.2, 0.4, 0.6, 0.8, and 1 M, while the protein concentrate was prepared using the salting out method with a saturation level of the ammonium sulfate salt of 55%, 60%, and 65%. Analysis of protein content was carried out using the Kjeldahl method. The use of NaOH 0.2 M and (NH4)2SO4 with a saturation level of 60% was the chosen treatment with crude protein content and yield of 75.63% and 33.83%, respectively.
Penentuan Kadar Albumin Ikan Gabus (Channa striata) dengan Metode Biuret setelah Dikukus dan Dipepes: Determination of Albumin Content of Snakehead Fish (Channa striata) by Biuret Method after Steamed and Wrapped Inda Hastuti N Naser; Syaiful Bahri; Pasjan Satrimafitrah; Abd. Rahman Razak; Ruslan; Ahmad Ridhay; Dwi Juli Puspitasari; Khairuddin
KOVALEN: Jurnal Riset Kimia Vol. 9 No. 2 (2023): August Edition
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/kovalen.2023.v9.i2.16391

Abstract

Snakehead fish (Channa striata) is a type of freshwater fish that contains high levels of protein albumin. The research was conducted to determine the albumin content in snakehead fish after the cooking process, both steamed and wrapped. The research was conducted using a completely randomized design with a factorial pattern, the first factor was the cooking method (steamed and wrapped) and the second was cooking time (10, 15, 20, and 20 minutes). Determination of protein albumin levels in snakehead fish after processing using the Biuret method. The steaming process for 15 minutes resulted in protein content in the snakehead fish of 0.76%, while the protein albumin that was lost was 19.28%. The wrapped method for 20 minutes has a remaining protein content of 0.59%, while the missing protein is 38.78%. The method of processing snakehead fish by steaming is better than the wrapping process.
Isolation and Characterization of Flavonoid Derivative of Ethyl Acetate Extract from Bauhinia latisiliqua Stem Bark and Its Activity as Antioxidant Sitti Hadijah Sabarwati; Indriani Indriani; Nurhaeni Nurhaeni; Ahmad Ridhay; Pasjan Satrimafitrah; Jaya Hardi; Rismawaty Sikanna
al Kimiya: Jurnal Ilmu Kimia dan Terapan Vol 10, No 2 (2023): al Kimiya: Jurnal Ilmu Kimia dan Terapan
Publisher : Department of Chemistry, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ak.v10i2.30372

Abstract

Various bioactivities on natural compounds, the antioxidant potency can provide a broad spectrum in their utilization and development into medicinal raw material. One of the plants that have potency for antioxidant activity is the Kemerakan sapenit (Bauhinia latisilqua) plant. The study aimed to isolate, characterize, and determine the antioxidant activity of a compound isolated in the ethyl acetate extract of the stem bark of B. latisilqua. This research includes extraction, fractionation, purification, and elucidation of secondary metabolite structure as well as testing its antioxidant activity. Extraction was carried out by maceration technique, fractionation, and purification using liquid vacuum chromatography and gravitation column chromatography. The elucidation of the structural compound was determined by analysis of the UV, IR, and NMR spectra. The antioxidant test was performed using the DPPH method. Based on the analysis of the spectral data, the isolated compound was catechin, a derivative of flavan-3-ol. Antioxidant test on catechin compound showed a strong antioxidant with an IC50 value of 35.01 µg /mL (IC50 for positive control, ascorbic acid, of 42.94 µg /mL).
pH Effect, Isothermal Adsorption, and Adsorption Kinetic Study of Copper(II) Ion into PVA/Alginate Beads Containing Banana Peels Satrimafitrah, Pasjan; Inda, Nov Irmawati; Ketut Sumarni, Ni; Sarapun, Veky; Fridian Aprilia, Natasya; Shiomori, Koichiro
The Journal of Pure and Applied Chemistry Research Vol. 12 No. 3 (2023): September-December 2023
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2023.012.03.3319

Abstract

Banana peels have been widely used as a biosorbent for metal ion adsorption. However, the small size of banana peels as a biosorbent leads to difficulty in the separation process of banana peels from the aqueous solution. Thus, the banana peels were impregnated into PVA/Alginate beads to increase the biosorbent size using the (W/O) emulsion process. Various amounts of banana peels were impregnated into beads. The obtained beads were used to adsorb copper(II) ions from aqueous solutions. The result shows that beads could extract the copper(II) ion from an aqueous solution containing 0.7875 ppm copper(II) ion at pH 4 with the quantity of extraction was 0.729 mmol/g. The effect of contact time and initial concentration indicates that the adsorption process of copper(II) ion from aqueous solution using beads follows the Langmuir isothermal adsorption and the adsorption kinetic follows the pseudo-second-order equation. Hence, the adsorption mechanism follows a chemisorption process with monolayer formation on the PVA/Alginate containing banana peels. Further investigation was carried out and found that the surface reaction and film diffusion occurred at initial stage of contact time, adsorptive diffusion through pores occurred at the later stage.
Co-Authors Abd. Rahman Razak Abd. Rahman Razak Abd. Wahid Wahab Ahmad Ridhay Ahmad Ridhay Aini Auliana Amar Angriani Sambali Dwi Juli Puspitasari Dwi Juli Pusptasari ellditna jenianti Enni Indriani Fridian Aprilia, Natasya Hardi Ys Hasdinda Hasdinda Hasnah Natsir Ikatami Putri Lestari Inda Hastuti N Naser Inda, Nov Irmawati Indriani Indriani Indriani Jaya Hardi Jaya Hardi Jusman, Jusman Khairuddin Khalil Mubarak Mappiratu Mappiratu Mohamad Afdal Musafira Musafira Ni Kadek Atmiyanti Ni Ketut Sumarni Nina Rahmadani Nov Irmawati Inda Nurhaeni Nurhaeni Nurhaeni Nurhaeni Nurhaeni NURUL CHOLIFAH Prismawiryanti Prismawiryanti Rismawaty Sikanna Ruslan Ruslan Ruslan Sarapun, Veky Shiomori, Koichiro SITTI HADIJAH SABARWATI Syaiful Bahri Syaiful Bahri Syaiful Bahri