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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Pendidikan Biologi Undiksha Jurnal Penelitian Pendidikan IPA (JPPIPA) Biologica Samudra PROFICIO: Jurnal Pengabdian Masyarakat Farmers: Journal of Community Services Maximus: Journal of Biological and Life Sciences
Yanti Ariyanti, Yanti
Department of Biology, Faculty of Mathematic and Natural Sciences, Bogor Agricultural University, Bogor 16680, Indonesia.
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Physics Social Sciences Veterinary Other

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DNA Barcoding for Selected Mangrove-Based Estuary Fishes from Way Kambas National Park, Lampung Province, Indonesia Ariyanti, Yanti; Rini, Ika Agus; Oktaviani, Indah; Leksikowati, Sovia Santi
Journal of Tropical Life Science Vol 11, No 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.02.04

Abstract

Over the past decade, DNA barcoding has provided new insight into fish ecology and biosystematics and led to new species' discovery. DNA barcoding is a method for the recognition and identification of species using short, standardised DNA fragments. The correct taxonomic identification of species is critical for the assessment and monitoring of biodiversity. This study applied DNA barcoding techniques to identify selected fish species from a mangrove-based estuary in Way Kambas National Park, Lampung Province, Indonesia. The gene encoding cytochrome c oxidase subunit I (COI) was amplified and bi-directionally sequenced from 22 specimens. The resulting 680 base pairs (bp) sequence was used to identify species, obtain phylogenetic information, and analyse genetic distances. A neighbour-joining tree was constructed based on the mitochondrial COI gene using the Kimura two-parameter model. This study also exhibits conservation status for those identified species. Our findings will facilitate future studies of fish species diversity in mangrove estuary-based ecosystems and provide preliminary data in policymaking in conservation areas such as National Park.
Co-Authors Adhha, Nurul Andreas, Dicky ANDY DARMAWAN Anisa, Hida Arliani Nur Azmi, Sarah Hariyati, Dewi Ika Agus Rini, Ika Agus Imaniar, Lisana Husna Jaya, Bima Zein Leksikowati, Sovia Santi Maulana, Muhammad Indra Mu'awanah, Erina Mulyana, Jeane Siswitasari Novriadi Nur Hidayah Nurhayu, Winati Oktaviani, Indah Pakpahan, Suhendra Riana, Elisa Nurma Sari, Dian Anggria Sattia, Ana Simamora, Irma Dermawan Tarigan, Marta Rosi Br Veithzal Rivai Zainal Yeni Rahayu