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Pengaruh fungisida asam fosfit, dimetomorf, dan metalaksil terhadap perkecambahan konidia Peronosclerospora maydis dan intensitas penyakit bulai jagung Ginting, Cipta; Fauzia, Dita Nur; Swibawa, I Gede; Prasetyo, Joko
Jurnal Proteksi Agrikultura Vol. 2 No. 2 (2025): NOVEMBER, JURNAL PROTEKSI AGRIKULTURA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpa.2234-41

Abstract

Downy mildew is one of the main diseases in maize which can reduce production. The objectives of this study were to determine the effect of phosphorous acid, dimetomorph, and metalaxyl fungicides on the germination and tube length of Peronosclerospora maydis conidia and the effect of phosphorous acid, dimetomorph, and metalaxyl fungicide treatment on conidia on the intensity of downy mildew in plants inoculated with these conidia. This study consisted of two tests, i.e., in vitro tests (effect on conidia germination and tube length) and in vivo tests (effect on downy mildew intensity). In both tests, a randomized block design (RBD) was used with four treatments and six replications. The results showed that phosphorous acid and dimethomorph fungicides inhibitted germination and reduced the length of conidia sprout tubes and suppressed downy mildew intensity. Meanwhile, metalaxyl fungicide was not effective in suppressing germination and conidia tube length and the intensity of downy mildew.
Kemampuan produk bioinsektisida komersial berbahan aktif Metarhizium sp. dalam menyebabkan kematian larva Oryctes rhinoceros L. Fakhrurreza, Moch Fakhmi; Fitriana, Yuyun; Suharjo, Radix; Swibawa, I Gede
Jurnal Proteksi Agrikultura Vol. 1 No. 1 (2024): MAY, JURNAL PROTEKSI AGRIKULTURA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpa.1114-19

Abstract

The rhinoceros beetle (Oryctes rhinoceros L.) (Coleoptera: Scarabaeidae) is a significant pest reported to attack nearly all coconut and oil palm plantations in Indonesia. Metarhizium sp., a well-known biological control agent, has broad-spectrum insecticidal properties due to its production of destruxin toxins, which are particularly toxic to O. rhinoceros. However, the effectiveness of dry-formulation bioinsecticides containing Metarhizium for controlling this pest has not yet been fully assessed. Therefore, this study was conducted to evaluate the potential of a dry-formulated Metarhizium sp. bioinsecticide to induce mortality in O. rhinoceros larvae. The research objectives were: 1) to assess the ability of the dry formulation bioinsecticide with Metarhizium as the active ingredient to induce mortality in O. rhinoceros larvae; 2) to determine the most effective dose of the dry formulation bioinsecticide with Metarhizium sp. for causing high mortality rates in O. rhinoceros. The study employed a Completely Randomized Design (CRD) with five treatments: 37.5 g (P1), 28.13 g (P2), 18.75 g (P3), and 9.38 g (P4) of Metarhizium sp., along with a control (P5). Each experimental unit consisted of a 1000 ml jar containing 500 g of coconut stem powder and five O. rhinoceros larvae, replicated five times with a total of 125 larvae. Analysis was conducted using the LSD test at a 5% significance level. Results indicated that the bioinsecticide was effective, with the most effective dose being 18.75 g, which resulted in 76.00% mortality.
Pengaruh modifikasi media S terhadap produksi spora, viabilitas, dan patogenisitas jamur agensia hayati Mu’arif, Imam Al; Fitriana, Yuyun; Suharjo, Radix; Swibawa, I Gede
Jurnal Proteksi Agrikultura Vol. 1 No. 1 (2024): MAY, JURNAL PROTEKSI AGRIKULTURA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpa.1134-45

Abstract

Biological control fungi can be stored and produced in the form of spores on a specific medium. For mass production, a large quantity of spores is required in a short amount of time. This study aims to determine the effect of medium S on the sporulation, spore viability, and pathogenicity of the fungi Beauveria bassiana, Metarhizium flavoviride, Purpureocillium lilacinum, and Trichoderma asperellum. There were two sets of experiments in this study. The first experiment tested the growth ability of fungal isolates on modified medium S in vitro. The second experiment tested the viability of the fungi that had been grown on the medium used in the previous experiment. The results showed that the type of fungus and the type of medium affected growth and spore production but did not influence spore viability. Among the biological agents, there appears to be mutual inhibition, though this inhibition does not seem to affect their ability to cause the death of the test insects. The consortium of biological agents resulted in a higher mortality rate of the test insects compared to single-agent applications.
Sekolah Lapang Pemanfaatan Kompos Limbah Buah Kakao dan Bonggol Pisang di Perkebunan Kakao di Kecamatan Air Naningan Kabupaten Tanggamus Evizal, M.S., Dr. Ir. Rusdi; Hariri, Agus M.; Nurdin, Muhammad; Swibawa, I Gede; Sugiatno, Sugiatno; Prasmatiwi, Fembriarti Erry
Jurnal Pengabdian Kepada Masyarakat Sakai Sambayan Vol. 7 No. 3 (2023)
Publisher : Lembaga Penelitian dan Pengabdian Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jss.v7i3.444

Abstract

Limbah kulit buah kakao yang dibiarkan membusuk di kebun menimbulkan masalah sanitasi kebun kakao. Pisang merupakan tanaman tumpangsari utama di perkebunan kakao di Lampung dan bonggolnya merupakan limbah pada penjarangan tanaman pisang. Pengabdian kepada masyarakat ini bertujuan untuk meningkatkan pengetahuan dan keterampilan petani dalam pembuatan dan pemanfaatan kompos limbah kulit buah kakao dan bonggol pisang di perkebunan kakao. Kegiatan ini yang dilaksanakan di Desa Sidomulyo Kecamatan Air Naningan, menerapkan metode sekolah lapangan sehingga memerlukan jadwal pertemuan sesuai dengan topik yang disepakati. Kegiatan pengabdian kepada masyarakat ini telah terlaksana dengan lancar, mendapat dukungan petani, dan menawarkan soluasi terkait dengan sanitasi kebun kakao, pemanfaatan limbah buah kakao dan bonggol pisang, dan penyediaan bahan organik untuk mengkonservasi kesuburan tanah dan kesehatan agroekosistem. Kegiatan ini dapat meningkatkan pengetahuan dan keterampilan petani terkait topik pembuatan dan pemanfaatan kompos. Hasil post-test wawancara menunjukkan 90-100% peserta mampu menjawab pertanyaan. Kata kunci: Agroekosistem, bonggol pisang, biochar, kompos, kulit buah kakao, sanitasi, sekolah lapang
Preferensi Kumbang Beras (Sitophilus oryzae L.) pada Tujuh Varietas Jagung Rahman, Moch Rifki Andika; Pramono, Sudi; Solikhin, Solikhin; Swibawa, I Gede
Jurnal Proteksi Agrikultura Vol. 1 No. 2 (2024): NOVEMBER, JURNAL PROTEKSI AGRIKULTURA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpa.2146-55

Abstract

The rice weevil (Sitophilus oryzae L.) is a primary pest of rice but also has the capacity to infest other grain commodities, such as maize.The objective of this study is to determine the preference of rice weevils to seven different maize varieties.The research was conducted at the Plant Pest Science Laboratory, Faculty of Agriculture, University of Lampung, from March to August 2024.The preference test was assessed using the Free Choice Test Method (FCTM). The experiment involved the placement of 30 g of each maize variety in separate treatments, with 30 pairs of S. oryzae positioned at the center of the cage. This design ensured equal opportunity for the weevils to select any of the maize varieties. The preference test spanned seven days, after which each maize variety was subjected to an incubation period of one month. The observed variables included the preference or number of rice weevil imago (both male and female), the number of first-generation offspring, median development time, sensitivity index, percentage of damage, and weight loss of maize.The research results indicated that S. oryzae exhibited the highest preference for the Kristal Madura maize variety compared to six other maize varieties. The highest number of male and female imago, as well as first-generation offspring, was observed in the Kristal Madura variety.The susceptibility index of the Kristal Madura variety was the highest, making it the most vulnerable variety.The highest percentage of weight loss was observed in the Kristal Madura and Kretek Madura varieties.
Potensi jamur Purpureocillium lilacinum sebagai endofit pengendali nematoda puru akar pada tanaman tomat (Solanum lycopersicum L.) Pamungkas, Dimas Bagus; Swibawa, I Gede; Aeny, Titik Nur; Pramono, Sudi
Jurnal Proteksi Agrikultura Vol. 1 No. 2 (2024): NOVEMBER, JURNAL PROTEKSI AGRIKULTURA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpa.2195-101

Abstract

This research aims to study the effectiveness of the fungus Purpureocillium lillacinum as a control agent for root-knot nematodes and its potential as an endophyte in tomato plants. The study was conducted from August 2023 until Februaty 2024 in the greenhouse of the Integrated Field Laboratory, Faculty of Agriculture, University of Lampung. The experiment followed a Completely Randomized Design (CRD) consisting of five treatments with five replications. The treatments tested were different dosage levels of P. lilacinum fungus culture on rice; 5g, 10g, 20g, 20g, 40g and 0g per polybag containing 2.5 kg of planting medium. P. lilacinum was applied by spreading it on the planting medium and soaking the roots in a suspension of fungal conidia before transplanting. Seven days after transplanting, the tomato plants were infested with 2000 root-knot nematode (RKN) Meloidogyne sp. eggs. Ninety days after planting, the plants were harvested to observe nematode populations in the roots and soil, root damage, and fungal colonization in the roots. Nematode (J2) in roots were extracted using a modified Baerman method from 5g of root samples, while nematode (J2) in the soil were extraced by sieving and sentrifugation with a sugar solution from 300 cc of soil. Root damage was assessed using a root gall score 0-10, and fungal colonies in the roots was expressed as a percentage after staining using tryphan blue. Data were analyzed for variance and followed by the Least Significant Difference (LSD) test for at a 5% significance level. The results showed that the application P. lilacinum at 40 g per plant was effective in reducing root-knot nematode damage, as well as decreasing RKN (J2) populations in the soil and roots. Additionally, P. lilacinum was confirmed to be endophyte in tomato plants.
Uji Efektivitas Trichoderma asperellum dalam Pengendalian Nematoda Puru Akar (Meloidogyne spp.) Secara In Vitro Indriyani, Sisi; Swibawa, I Gede; Dewi, Ni Kadek Emi Sintha; Ginting, Cipta
Jurnal Proteksi Agrikultura Vol. 3 No. 1 (2026): MAY, JURNAL PROTEKSI AGRIKULTURA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpa.1315-20

Abstract

Root-knot nematodes (Meloidogyne spp.) are significant pests that damage the root systems of plants and are difficult to control using conventional methods. The use of chemical nematicides carries the risk of environmental contamination, so environmentally friendly control alternatives are needed, one of which is using the biological agent Trichoderma asperellum. This study aims to determine the effect of spore concentration and exposure duration of T. asperellum on the hatching of Meloidogyne spp. eggs in vitro. The research was conducted at the Plant Pest Science Laboratory and the Biotechnology Laboratory of the Faculty of Agriculture, University of Lampung, from May to July 2025. The experiment used a completely randomized design (CRD). The first experiment tested five concentrations of spore suspension dilutions (10-1, 10-2, 10-3, 10-5, and a control), while the second experiment tested exposure durations of 10, 15, 30, and 60 minutes. The results showed that a concentration of 10-2 (2.7 × 105 spores/mL) was able to inhibit egg hatching by 50.90%, which was higher than the other concentrations. In the duration test, juvenile mortality increased over time, reaching 85.37% at 10 minutes and 99.83% at 60 minutes. In conclusion, a 10-2 concentration with a minimum exposure of 30–60 minutes is effective in reducing the hatching rate of Meloidogyne spp. eggs and has the potential to be applied as an environmentally friendly biological control.
Pengaruh implementasi Netamax-FP Unila terhadap keragaman dan kelimpahan nematoda dalam tanah pada pertanaman guava kristal di Lampung Saharani, Aulia Shalsha; Swibawa, I Gede; Helina , Selvi; Suharjo, Radix
Jurnal Proteksi Agrikultura Vol. 3 No. 1 (2026): MAY, JURNAL PROTEKSI AGRIKULTURA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpa.1321-30

Abstract

Crystal guava (Psidium guajava L.) is a horticultural plant whose fruit is widely known and as a favorable fruit in Indonesia. Crystal guava productivity in Lampung is relatively lower compared to that in Central Java and East Java. One of the factors causing a decline in crystal guava production is pest attack, including plant-parasitic nematodes. The aim of this study was to determine the effect of Netamax-FP Unila bionematicide application on the composition of the nematode community and its effects on the diversity and abundance of nematodes in crystal guava plantations at PT Great Giant Food. The research was conducted from September 2023 to March 2024. Nematode observations were carried out before and after application bionematicide. Nematodes were identified to the genera taxonomic level. Nematode diversity was measured using the number of genera, funtional groups, the Shannon-Wiener index, species richness index, species evenness index and Simpson's index. Nematode abundance was measured base on the number of individuals of each genus. The research results showed that 16nematode genera were identified. These genera were classified into three functional groups, including parasitic nematodes (herbivores), bacterial-feeding nematodes (bacterivores), and fungal-feeding nematodes (fungivores). The application of the Netamax-FP Unila bionematicide combined with compost reduced the abundance of plant-parasitic nematodes without having a negative impact on free-living nematodes or overall nematode diversity.
Co-Authors Agus M. Hariri Agus Muhammad Hariri, Agus Muhammad Anak Agung Gede Sugianthara Aulia, Shifa Veronica Bainah Sari Dewi Basuki, Muh. Cipta Ginting, Cipta Dad Resiworo Sembodo, Dad Resiworo Dewi, Ni Kadek Emi Sintha Efri, Efri Erwin Yuliadi Evizal, M.S., Dr. Ir. Rusdi F.X. Susilo, F.X. Fakhrurreza, Moch Fakhmi Fauzia, Dita Nur Fembriarti Erry Prasmatiwi Fiandani, Ambar Firdaus, Fazri Fitriyani, Novi Nur Franciscus Xaverius Susilo, Franciscus Xaverius Gunito, Heru Hamim Sudarsono . Hariri, Agus M Hartaman, Maman Helina , Selvi Helina, Selvi Imawati Imawati, Imawati Indriyani, Sisi Indriyati . Joko Prasetyo Kaneko, Nobuhiro Kristina, Anastasia Kumbara, A. M. Lestari Wibowo M Hariri, Agus Marlinawati, Fransiska Dina Merdiana, Eryka Muhammad Nurdin Mu’arif, Imam Al Nabilah Nabilah Oktavia, Tiara Pamungkas, Dimas Bagus Panjerrino, Yoshua Gdemakarti PUJI LESTARI Purnomo Purnomo Radix Suharjo Rahma Dhona, Alfira Rahman, Moch Rifki Andika Ratdiana, Ratdiana Ringo, Andrew Y. C. Rosma Hasibuan, Rosma Ruruh Anjar Rwandini, Ruruh Anjar Saefudin Saefudin Saharani, Aulia Shalsha Saputra, Muhamad Jaya Saputra, Oded Setyo Dwi Utomo Sholih, Muhammad Badrus Solikhin ., Solikhin Solikhin Solikhin Sri Haryani Sudi Pramono . Sugiatno Sugiatno Suputa Suputa Susilo, F X Susilo, Fransiscus Xaverius Susilo, FX. Suskandini Ratih Dirmawati Syahrani, Kristinda Titik Nur Aeny Tri Maryono Wardiyani, Wardiyani Widayati, Wiludjeng Wilandari, Riri Windriyanti, Wiwin Yuyun Fitriana Zahra, Fatimah Az