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Enrichment Media Selection and Co-Culture Potential among Exoelectrogen Bacteria Vary with Ecological Factors Khoirunnisa, Nur Syafira; Anwar, Syaiful; Sudadi, Untung; Santosa, Dwi Andreas
Makara Journal of Science Vol. 27, No. 1
Publisher : UI Scholars Hub

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Abstract

Staphylococcus saprophyticus ICBB 9554 and Citrobacter freundii ICBB 9763 are exoelectrogen bacteria applied as microbial fuel cells (MFC). We selected enrichment media for both these exoelectrogen bacteria, characterized their synergistic traits, and evaluated the growth conditions under different ecological factors. In this study, different enrichment media, such as those containing sugar, molasses, and palm sugar (2, 4, and 6% w/v) were tested for S. saprophyticus ICBB 9554. Meanwhile, technical sodium acetate (NaAc), commercial vinegar 25%, and cider vinegar (5, 10, and 15 mM acetate concentration) were tested for C. freundii ICBB 9763. Both the exoelectrogen bacteria were tested for the synergistic trait by inhibitory test, metabolic activity, and electricity generation performance in MFC. Different ecological factors, including salinity (2, 3, and 4 dS/m), culture media pH (5.8, 6.5, and 6.9), and temperature (20 °C, 27 °C, and 31 °C) were investigated for bacterial growth. The selective enrichment medium ingredient for S. saprophyticus ICBB 9554 was 2% molasses while that for C. freundii ICBB 9763 was 0.05 M technical sodium acetate. Both bacteria were suspected to grow synergistically and could be inoculated simultaneously as a co-culture in MFC. The effect of different ecological factors indicated that co-culture could grow better than pure culture. This study's findings provide important data on potential enrichment media for exoelectrogen bacteria that are beneficial for scale-up to reduce the operational cost.
Pengaruh Bulai pada Perubahan Indeks Kadar Klorofil, Serapan Fosforus dan Boron pada Jagung Manis Achmad Azhari Sidik; Budi Nugroho; Untung Sudadi
Jurnal Ilmu Pertanian Indonesia Vol. 29 No. 4 (2024): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.29.4.667

Abstract

Maize production and quality are affected by infection with plant pathogens. One of the maize's essential and main diseases is downy mildew caused by Peronosclerospora spp. Downy mildew is a limiting factor in increasing production and can reduce production by 80-100%. It is because the affected plant cannot produce cobs. Pathogens obtain nutrients from the host cell, which can kill the cell and damage the surrounding tissues, resulting in visible downy mildew symptoms. Boron (B) plays a role in forming phloem, increasing the fruit's number, weight, bunch weight, and diameter. The primary function of B at the molecular level is the cross-linking of pectin in the plant cell wall. Ramnogalacturonan II (RG II) is a pectic polysaccharide that contributes to the mechanical strength and properties of the primary wall cross-linked by borate diesters. Phosphorus (P) controls the downsides in the greenhouse and field conditions. This study aims to measure changes in chlorophyll index, P and B uptakes in downy mildew affected plants. The field experiment used a group randomized design with six natural phosphate (FA) application treatments and four groups of borax doses as replicates. The results showed that the downy mildew decreased the chlorophyll index of the leaves at different levels of attack. The results of P concentration according to the position of healthy plant leaves were significantly different due to P treatment. In contrast to concentration B, there is no real difference. P and B uptake results in downy mildew-infested plants showed a significant difference only in P uptake in leaves with 1 FA treatment. Keywords: boron, downy mildew, maize, phosphate
Amelioration of Potassium Humate as Ligand Exchanger for Adsorbed P Fractions and their Relationships with Chemical Properties of an Indonesian Andisol Putri, Elsa Kumala; Suwardi, Suwardi; Sudadi, Untung
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol 14 No 4 (2024): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Pusat Penelitian Lingkungan Hidup, IPB (PPLH-IPB) dan Program Studi Pengelolaan Sumberdaya Alam dan Lingkungan, IPB (PS. PSL, SPs. IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.14.4.875

Abstract

Andisols are specifically characterized by a very high P adsorption capacity owing to their acidic reaction and dominance of variable-charged clay minerals. This crop cultivation problem can be overcome by potassium humate amelioration intended to exchange the adsorbed soil P fractions into the more available (labile) forms. This study aims to evaluate the effects of potassium humate application at 0, 20, 40, and 60 kg ha-1 on the dynamics of P fractions and their relationships with the chemical properties of an Indonesian Andisol. Using the Tiessen and Moir procedure, PH2O, PNaHCO3-Pi-Po, PNaOH-Pi-Po, and PHCl fractions were obtained in topsoil (0–30 cm) and subsoil (30–60 cm) samples 16 weeks after the treatment. The organic soil P (PPo) fractions tended to increase and were attributed positively to total P and negatively to base saturation (BS) at the topsoil, whilst the subsoil PPo fractions tended to increase with the decreasing soil BS. At 60 kg ha-1, the potassium humate application effectively decreased PPo and increased PPi fractions in the subsoil. The results also revealed that humic acid component of potassium humate was an effective organic ligand exchanger for the adsorbed PPo fractions that simultaneously altered soil anion-cation equilibrium in the studied Andisol. As an implication, soil P availability was increasing.
Dynamics of Tropical Peatlands Characteristics and Carbon Stocks as Affected by Land Use Conversion and Ages of Land Use in Riau Province, Indonesia Juliano, Gian; Suwardi, Suwardi; Sudadi, Untung
JOURNAL OF TROPICAL SOILS Vol 29, No 1: January 2024
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2024.v29i1.23-32

Abstract

The dynamics and patterns of relationships between the characteristics of Indonesian tropical peat involving a function of time (ages of land use) in the context of land conversion have yet to be widely reported. The effects of tropical peatland use change are generally discussed regarding C emissions, with the limited literature identifying dynamic soil characteristics as these changes occur. This study used a survey method. We identified the physical, chemical, and C stock characteristics of peat in forest locations, oil palm plantations with a land use age of 0-5 years,>5-10 years,>10 years, and agricultural land with a land use age of 0-5 years and >5-10 years to find out the dynamics of soil characteristics. Land use changes from peat swamp forests to oil palm plantations and agricultural land, and the longer age of land use results in an increase in BD (especially at the top layer), ash content, pH, Total-N, total-P2O5, total-K2O, but there was a decrease in water content, fiber content, Organic-C, E400/E600, and soil C stocks. The alignment of economic, social, and ecological interests was directed to water and land management by regulating the area’s hydrological system and increasing peat stability.
Short-term natural restoration effects on groundwater table in several peatland conditions of Acacia plantation forests Qalbi, Andria Harfani; Tarigan, Suria Darma; Barus, Baba; Sudadi , Untung
Journal of Degraded and Mining Lands Management Vol. 12 No. 4 (2025)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2025.124.8191

Abstract

One significant approach to restore the ecosystem function of degraded peatlands is natural restoration. This study evaluated the effect of short-term natural restoration on groundwater table in several peatland conditions in the Acacia plantation retired since 2016. Four types of land: burned land, Acacia post-harvest land, retired Acacia plantation forest, and the natural forest were considered as our study area. This study analyzed soil properties and remote sensing using the NDVI index to evaluate vegetation density in 2016, 2019, and 2022. This study found that natural restoration in the period of 2016-2022 increased vegetation density, almost similar to natural forest vegetation. Both soil properties and groundwater table on Acacia plantation forest and natural forest were not significantly different. High saturated hydraulic conductivity and low bulk density on both areas supported the stability of peat hydrology. In addition, variations in rainfall affected the pattern of changes in groundwater table, with a decrease during the dry season and an increase during the rainy season. The findings of this study confirm that short-term natural restoration can restore hydrological and vegetation functions in degraded peatlands, especially in Acacia plantation areas. These findings can be scientific recommendations for sustainable peatland management.
Inaktivasi In Situ Pencemaran Kadmium pada Tanah Pertanian Menggunakan Amelioran dan Pupuk pada Dosis Rasional untuk Budidaya Tanaman Sudadi, Untung; Sabiham, Supiandi; Sutandi, Atang; Saeni, Muchammad Sri
JOURNAL OF TROPICAL SOILS Vol. 13 No. 3: September 2008
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2008.v13i3.171-178

Abstract

Arable soils located in the vicinity of urban and industrial area are considered vulnerable to heavy metals pollution.  Chemical inactivation, an in situ remediation method that used inexpensive chemicals to reduce pollutant solubility in polluted soil, was examined on a Cd-spiked arable soil. The study was aimed at to investigate the effectiveness of ameliorants and fertilizers normally recommended for crop cultivation, using tomato as the test plant, from the point of view to reduce soil Cd extractability and plant Cd concentration. A 100-day greenhouse experiment in completely randomized de­sign consisting of three rates of rationale dosage of ameliorants and fertilizers (RDAF) [0, 50, 100%] and four levels of soil Cd spike [0, 10, 20, 40 mg Cd kg-1 soil, using 3CdSO4.8H2O] in triplicate was conducted. The 100% rate of RDAF applied was: 4 ton dolomite ha-1, 30 ton cow dung ha-1, 150 kg N ha-1 (½ Urea + ½ Ammonium Sulfate), 150 kg P2O5 ha-1 (SP-36) and 100 kg K2O ha-1 (KCl). Significant reductions in soil extractable Cd (CdNH4OAc-EDTA) [from 13.35 to 8.77 mg kg-1, 34%] and plant shoots-Cd (Cdps) [from 8.66 to 5.46 μg g-1, 37%] were measured at 100% RDAF treatment as compared to the control soil, indicating the occurrence of an in situ soil Cd inactivation and plant element selective-uptake phenomenon. Multiple regression analysis that incorporating all 36 data pairs resulted in the following equations: (1) soil CdNH4OAc-EDTA = – 0.18 pHH2O + 0.21 PBray#1 + 0.51 organic-C – 0.57 exch.-Mg + 0.65 Cdaqua regia (R2 = 0.76), and (2) Cdps = 0.08 Nps – 0.17 Kps – 0.19 Mgps – 0.24 Pps + 0.27 Sps – 0.41 Caps (R2 = 0.54, 3 outliers removed). These results suggest that a proper amelioration and fertilization program may be prospective to be recommended as a low-cost Cd pollution remediation strategy for arable soils
Dynamics of Tropical Peatlands Characteristics and Carbon Stocks as Affected by Land Use Conversion and Ages of Land Use in Riau Province, Indonesia Juliano, Gian; Suwardi, Suwardi; Sudadi, Untung
JOURNAL OF TROPICAL SOILS Vol. 29 No. 1: January 2024
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2024.v29i1.23-32

Abstract

The dynamics and patterns of relationships between the characteristics of Indonesian tropical peat involving a function of time (ages of land use) in the context of land conversion have yet to be widely reported. The effects of tropical peatland use change are generally discussed regarding C emissions, with the limited literature identifying dynamic soil characteristics as these changes occur. This study used a survey method. We identified the physical, chemical, and C stock characteristics of peat in forest locations, oil palm plantations with a land use age of 0-5 years,>5-10 years,>10 years, and agricultural land with a land use age of 0-5 years and >5-10 years to find out the dynamics of soil characteristics. Land use changes from peat swamp forests to oil palm plantations and agricultural land, and the longer age of land use results in an increase in BD (especially at the top layer), ash content, pH, Total-N, total-P2O5, total-K2O, but there was a decrease in water content, fiber content, Organic-C, E400/E600, and soil C stocks. The alignment of economic, social, and ecological interests was directed to water and land management by regulating the area’s hydrological system and increasing peat stability.
Typology of artisanal and small-scale gold mining sites along the Singingi River, Riau Province Okalia, Deno; Iskandar; Mulyanto, Budi; Sudadi, Untung
Journal of Degraded and Mining Lands Management Vol. 12 No. 5 (2025)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2025.125.8719

Abstract

Artisanal and small-scale gold mining (ASGM) in the Singingi Watershed has precipitated a multifaceted transformation of the landscape, resulting in severe geomorphological, sedimentological, and hydrological alterations. This study leveraged an integrative quantitative descriptive framework and advanced spatial analysis to systematically delineate post-mining land typologies along a 51.25 km stretch of the watershed, using high-resolution remote sensing data and validation from 243 strategically distributed sample points. Eight distinct land typologies (A-H) were identified based on detailed assessments of topographic attributes, particle size distribution, pit depth, and tailing mound characteristics. Notably, typology H—characterized by a flat terrain (slope <8%), shallow pit depths and tailing mound heights (<2 m), and a predominance of fine-textured soils—emerged as the most amenable to passive reclamation and natural vegetation regeneration. Overlay analyses further demonstrated that Sungai Paku Village harbors the largest expanse of typology H (108.16 ha), underscoring its critical priority for reclamation interventions. These findings provide a robust, evidence-based foundation for the development of adaptive reclamation strategies, offering valuable insights for restoring ecological and physical functions in mining-impacted environments. The study underscores the importance of integrating multidisciplinary spatial data to inform sustainable land management practices in regions severely affected by ASGM activities and as a reference for the community for reclamation priorities based on land typology.
Pengaruh Steel Slag, Fly Ash dan Bottom Ash terhadap Pertumbuhan Tanaman Padi di Tanah Gambut Purnamasari, Laili; Sudadi, Untung; Anggria, Linca; Hartono, Arief
Jurnal Ilmu Tanah dan Lingkungan Vol 26 No 1 (2024): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitl.26.1.48-53

Abstract

Kegiatan industri di Indonesia menghasilkan limbah seperti steel slag, fly ash dan bottom ash yang mengandung hara makro, mikro dan benefisial. Limbah industri tersebut mampu meningkatkan kualitas tanah seperti tanah gambut. Namun bahan limbah tersebut juga mengandung logam berat beracun yang perlu dikaji. Penelitian ini bertujuan untuk mengetahui pengaruh steel slag, fly ash dan bottom ash terhadap sifat kimia tanah gambut, pertumbuhan padi (varietas IR 64), hasil produksi dan kandungan logam berat beracun pada beras. Bahan pembenah tanah yang digunakan pada masing-masing perlakuan adalah 0; 2,5; 5,0; 7,5 dan 10,0% dari berat kering oven tanah. Percobaan inkubasi menunjukkan penambahan steel slag dan fly ash dapat meningkatkan pH tanah, ketersediaan Ca, Mg dan Si. Sementara itu, perlakuan bottom ashhanya sedikit memperbaiki sifat kimia tanah gambut. Pada percobaan rumah kaca, aplikasi steel slag, fly ash dan bottom ash pada tanah gambut dapat meningkatkan pertumbuhan tanaman padi dan hasil produksi. Respon positif padi berhubungan dengan peningkatan pH tanah gambut dan ketersediaan Si, Ca dan Mg yang berasal dari pembenah tanah. Beras yang dihasilkan tidak mengandung Pb tetapi mengandung Cd. Kandungan Cd pada beras masih berada dalam batas maksimum yang diizinkan untuk cemaran logam berat pada beras yang disosoh menurut SNI 6128:2020.
Aplikasi kleinano dari tuf volkan Gunung Salak Indonesia sebagai adsorben alami kontaminan anionik: Fosfat perairan Sudadi, Untung; Anggriawan, Rendy; Anwar, Syaiful
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol 9 No 4 (2019): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan
Publisher : Pusat Penelitian Lingkungan Hidup, IPB (PPLH-IPB) dan Program Studi Pengelolaan Sumberdaya Alam dan Lingkungan, IPB (PS. PSL, SPs. IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.9.4.1032-1040

Abstract

Indonesia is rich with volcanic tuff-derived Andisols that contain variable charged minerals. This research was aimed at to extract nanoclays (diameter <200 nm) from volcanic tuffs, and to evaluate their potential as natural adsorbent of phosphate as contaminant in waters. Volcanic tuffs were taken from the 3rd (tv3) and 4th(tv4) layers of an Andisol profile at the foot slope of Salak Mountain Indonesia using dispersion-ultrasonication-centrifugation-dialysis separation procedure. By dispersing the materials in an acid condition (pH 4.0) followed with ultrasonication, it could be separated positively-charged nanoclays (nc3 and nc4) from the negative ones. The separated nanoclays were then purified using centrifugation and membrane dialysis techniques. Potential of the extracted nanoclays as natural phosphate adsorbent was evaluated by characterizing their adsorption maxima (b values) according to the Langmuir isothermal adsorption model. It could be extracted 2.82 mg nc3/g tv3 and 4.29 mg nc4/g tv4. The b values of nc3 at 12, 24, and 48 hours equilibration time (76.48, 92.10, and 117.54 mg P/g nc3) were higher than those of nc4 (50.17, 59.52, and 71.99 mg P/g nc4). The most effective equilibration-time was 48 hours. The extracted nanoclays were considered prospective as natural adsorbent for removal phosphate contaminant in waters