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All Journal Jurnal Ampere PENDIPA Journal of Science Education JUTIS : Jurnal Teknik Informatika Pelita Eksakta Suluah Bendang: Jurnal Ilmiah Pengabdian Kepada Masyarakat Jurnal Abdimas Bina Bangsa Publikasi Hasil Pengabdian Kepada Masyarakat. UNP Journal of Statistics and Data Science Indonesian Journal of Statistics and Its Applications
Dina Fitria, Dina
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Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Education Electrical & Electronics Engineering Engineering Languange, Linguistic, Communication & Media Mathematics Physics Social Sciences Other

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Journal : PENDIPA Journal of Science Education

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Pemeriksaan TiLV (Tilapia Lake Virus) Pada Ikan Nila (Oreochromis niloticus) Budidaya Fitria, Dina; Fathonah, Deffya; Afrianti, Rini; Turrohmah, Azizah; Mujaidiah; Destiana, Anggari Linda; Day, Dafzel; Astuti, Indri
PendIPA Journal of Science Education Vol 9 No 2 (2025): June
Publisher : UNIB Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/pendipa.9.2.310-317

Abstract

Nile Tilapia (Oreochromis niloticus) is highly sought after by Indonesian consumers and is one of the leading commodities cultivated in freshwater. However, tilapia farming still frequently encounters challenges that lead to a decline in production, one of which is infection by pathogens such as Tilapia Lake Virus (TiLV). TiLV can infect fish in waters with temperatures above 25°C, with mass mortality peaking at 30°C, reaching a 50% death rate. Proper handling and control measures are essential to manage TiLV outbreaks effectively. TiLV detection in fish can be conducted using the Polymerase Chain Reaction (PCR) method. PCR-based virus detection is a diagnostic technique that identifies the presence of viral genetic material with high specificity and accuracy. This study aimed to detect TiLV infection in farmed Nile tilapia. The procedures included sample collection, necropsy, organ fixation, RNA extraction, PCR amplification, electrophoresis, and gel documentation analysis. Based on the PCR results, Nile tilapia samples tested positive for TiLV infection, as indicated by the presence of a 250 bp DNA band in the gel documentation system, aligning with the TiLV positive marker. Among the three fish showing suspected TiLV symptoms, two (66.6%) were confirmed infected.
Co-Authors Abdillah, Muhammad Mukhlis Abdul Aziz, Ahmad Malik Abdur Rahim, Abdur Al Aziz, Saddam Alamin, M. Anggari Linda Destiana Antasya, Faridah Darussalam, Muhammad Illyas Day, Dafzel Dewi Rawani Dinul Haq, Asra Dodi Vionanda Dony Permana Fathonah, Deffya Ghaly, Fayyadh Hendrawan, Muhammad Indri Astuti Joni Joni Khalida, Rizqiana Maulana, Muhammad Arif Moh. Wahyu Aminullah Moulita, R.A. Nurul Muhni Pamuji, Muhni Mujaidiah Mukhti, Tessy Octavia Nahdah, Athaya Nisrina Nonong Amalita Nugroho, Agung Pajrini, Nurul Hasni Permana, Dony Putri, Sindy Amelia Revelino, Imanuel Ridho, Fakhru Rini Afrianti Salma, Admi Suhady, Suhady Suherman, Suherman Suparmin Suparmin Syafriandi Syafriandi Turrohmah, Azizah Yarman Yarman, Yarman Yenni Kurniawati Yudi Irwansi, Yudi Yuwon, Yuwon zafira, Rufaidah Zamahsary Martha Zilrahmi, Zilrahmi