Garuda - Garba Rujukan Digital

Pengaruh Pemberian Ekstrak Buah Pala terhadap Kadar Eritrosit (RBC) dan Granulosit pada Tikus Putih (Rattus norvegicus) dengan Anemia Hasanah, Uswatun; Pangesti, Ira; Puspodewi, Dini
Jurnal Laboratorium Khatulistiwa Vol 9, No 1 (2025): November 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i1.2106

Anemia is a condition characterized by a decreased number of erythrocytes and an increased number of leukocytes. According to the 2023 Indonesian Health Survey, the prevalence of anemia across all age groups is 16.2%, with a higher rate in women (18%) compared to men (14.4%). Nutmeg (Myristica fragrans Houtt) contains bioactive compounds such as saponins, flavonoids, alkaloids, and essential fatty acids that may contribute to increasing red blood cell counts and modulating leukocyte levels. This study aimed to determine the effect of nutmeg fruit extract on increasing RBC and reducing granulocyte levels in anemic white rats. A total of 25 male rats (200–350 g) were divided into five treatment groups. Anemia was induced using NaNO₂ at a dose of 1.5 mg/kg BW orally. Nutmeg extract was administered at doses of 200 mg/kg BW, 300 mg/kg BW, and 400 mg/kg BW. There were no significant differences (p > 0.05) between the positive control and treatment groups, nor between pre- and post-extract administration. At doses of 200 and 300 mg/kg BW, RBC percentage reached 20.7%. For granulocytes, the 300 mg/kg BW dose produced a higher percentage (27.6%) compared to the 200 and 400 mg/kg BW doses. These findings suggest that nutmeg fruit extract has the potential to support erythrocyte balance in anemia conditions, although further research with larger samples and controlled dosing is needed.%.

CORRELATION OF HBA1C LEVELS TO LDL, HDL AND TRIGLYCERIDE LEVELS IN TYPE 2 DIABETES MELLITUS PATIENTS IN KESUGIHAN II PUBLIC HEALTH CENTER, CILACAP DISTRICT Lestari, Tika; Puspodewi, Dini; Farabi, Meka Faizal
Jurnal Mitra Kesehatan Vol. 8 No. 1 (2025): Jurnal Mitra Kesehatan
Publisher : STIKes Mitra Keluarga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47522/jmk.v8i1.421

Introduction: Diabetes mellitus or DM is a disease characterized by hyperglycemia due to abnormalities in insulin secretion, insulin action, or both. To avoid complications, patients with type 2 DM with uncontrolled blood glucose levels require comprehensive treatment. Poor glycemic control results in changes in lipid profiles in type 2 DM patients. The purpose of this study was to prove the correlation of HbA1c levels with LDL, HDL, and Triglyceride levels in Type 2 DM patients at the Kesugihan II Cilacap Health Center. Methods: This study design was cross-sectional. Examination of HbA1c, LDL, HDL, and Triglyceride without comorbidities of Heart, Hypertension, and Stroke. The sample was Prolanis Type 2 DM patients who met the inclusion and exclusion criteria. The analysis used for the normality test was the Shapiro-Wilk test and the nonparametric test used the Spearman test. Results: The Spearman test results between HbA1c levels and LDL levels showed a p value = 0.216, between HbA1c levels and HDL levels showed a p value = 0.123, between HbA1c levels and triglyceride levels showed a p value = 0.326. This shows that the probability is more than 0.05. The conclusion of this study is that there is no correlation between HbA1c levels with LDL, HDL and triglyceride levels in Prolanis DM Type 2 patients at the Kesugihan II Cilacap Health Center.

PERBANDINGAN HASIL HITUNG JUMLAH ERITROSIT MENGGUNAKAN TABUNG VACUTAINER EDTA BERDASARKAN SEBELUM DAN SESUDAH KADALUARSA 6 BULAN DAN 12 BULAN Endra Topan; Pangesti, Ira; Puspodewi, Dini
Pharmaqueous: Jurnal Ilmiah Kefarmasian Vol. 7 No. 1 (2025): Volume 7, Nomor 1, Mei 2025
Publisher : Universitas Al-Irsyad Cilacap

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36760/jp.v7i1.620

Erythrocytes or red blood cells are one of the components of blood that are dense shaped like a disc and do not have a nucleus with a size of 7-8 μm that is immobile, reddish-yellow and chewy so that it can change shape according to the blood vessels that are passed. If the blood collection vacutainer tube containing the anticoagulant is used past the expiration date, it is likely that the vacuum does not draw the required amount of blood, so the anticoagulant does not work effectively and does not prevent the blood from clotting it. Based on this background, the purpose of this study is to determine the comparison of the results of calculating the number of erythrocytes using edta vacutainer tubes based on before and after the expiration of 6 months and 12 months. The type of study is a descriptive analytical study by looking at whether there is a comparison of the results of calculating the number of erythrocytes using EDTA vacutainer tubes based on before and after the expiration of 6 months and 12 months with univariate and bivariate data collection and analysis techniques. Based on the results of statistical analysis, it can be seen that the average score of participants decreased from before the ED intervention (M = 4.559; SD = 0.310) to 4.453 (SD = 0.379) at 6 months after ED, and decreased again to 4.437 (SD = 0.438) at 12 months after ED. Despite this downward trend in mean values, the results of the Repeated Measures ANOVA test showed that this difference was not statistically significant (F(1.216) = 2.871; p = 0.086), although p was close to the significance threshold value (p < 0.05). This means that there was no significant change in the score between measurement times, either before or after the ED in the 6 and 12-month periods. Although statistical tests say there is no significant difference, checking the number of erythrocytes using vacutainer EDTA tubes before expiration is still recommended because descriptively there is an increase and decrease in the number of erythrocytes.

KORELASI KADAR ALBUMIN, AST (ASPARTATE TRANSAMINASE) DAN ALT (ALANINE TRANSAMINASE) PADA PASIEN TUBERKULOSIS DI RSU SANTA MARIA CILACAP Sari, Windia Arta; Puspodewi, Dini; Farabi, Meka Faizal
Pharmaqueous: Jurnal Ilmiah Kefarmasian Vol. 7 No. 1 (2025): Volume 7, Nomor 1, Mei 2025
Publisher : Universitas Al-Irsyad Cilacap

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36760/jp.v7i1.628

Tuberkulosis (TBC) adalah penyakit menular yang disebabkan oleh spesies Mycobacterium. Mycobacterium Tuberculosis, sejenis kuman berbentuk batang. Sebagian besar dinding kuman terdiri atas asam lemak (lipid), peptidoglikan dan arabinomanan yang menyebabkan kuman ini lebih tahan terhadap asam sehingga disebut bakteri tahan asam (BTA). Tujuan dari penelitian ini untuk mengetahui korelasi kadar Albumin, AST dan ALT pada pasien Tuberculosis di RSU Santa Maria Cilacap. Metode penelitian ini menggunakan studi analitik observasional dengan pendekatan cross-sectional untuk mengetahui korelasi kadar albumin, AST, dan ALT pada pasien TBC di RSU Santa Maria Cilacap ,pengambilan data menggunakan data sekunder dari Sistem Informasi Manajemen Rumah Sakit (SIM RS) pada periode September 2022 sampai dengan September 2023. Berdasarkan perhitungan rumus slovin, besar sampel yang dibutuhkan sebanyak 46 sampel. Sampel yang diambil berdasarkan teknik probability sampling tipe random sampling. Hasil penelitian menunjukkan bahwa rata-rata nilai Albumin (ALB) adalah 3.387 g/dL dengan rentang 2.110–4.690 g/dL. Rata-rata AST menunjukkan nilai yang sangat tinggi, yaitu 78.109 U/L dengan deviasi standar yang besar (158.941), yang mengindikasikan adanya pencilan atau distribusi data yang tidak normal. Demikian pula, ALT memiliki sebaran luas dari 5 hingga 605 U/L, dengan rata-rata 58.891 U/L. Hasil yang telah di uji tidak terdapat korelasi antara kadar albumin dengan AST maupun ALT, namun AST dan ALT memiliki hubungan yang signifikan dan sangat kuat (p-value < 0,001)

Subchronic Toxicity Test of Burdock Root (Arctium Lappa L.) Nanoemulsion Based on Histopathology on The Albino Rat Rattus noverticus (Wistar strain): Uji Toksisitas Subkronik Nanoemulsi Akar Burdock (Arctium Lappa L.) Berdasarkan Histopatologi pada Tikus Albino Rattus noverticus (strain Wistar) Imam Agus Faizal; Dini Puspodewi; Ajeng Puspo Aji
Indonesian Journal of Innovation Multidisipliner Research Vol. 2 No. 2 (2024): April - Juni
Publisher : Institute of Advanced Knowledge and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69693/ijim.v2i2.140

Tanaman burdock (Arctium lappa L.) merupakan tanaman herbal yang berkhasiat sebagai penambah darah (hemoglobin dan hematokrit) pada penderita anemia defisiensi besi. Sebanyak 15 ekor tikus (Rattus norvegicus) strain Wistar, jantan dan betina dibagi menjadi 3 kelompok secara random sampling yaitu Kelompok 1 Kontrol negatif (KN) tidak diberi A. lappa L. nanoemulsion. Kelompok Dosis 1 (KD1) diberi A. lappa L. nanoemulsion dengan dosis 150 mg/kgBB/hari. Kelompok Dosis 2 (KD2) diberikan A. lappa L. nanoemulsion dengan dosis 450 mg/kgBB/hari (selama 14 hari dimulai hari ke 8). Indikator penelitian ini dilihat kadar pemeriksaan biokimia kadar darah. Organ hati dan ginjal dinilai menggunakan Hematoxylin-Eosin (HE). Hasil pemeriksaan biokimia darah relatif normal. Sedangkan pewarnaan HE pada kelompok KD1 dan KD2 terjadi perubahan pada sel organ ginjal dan hati, menunjukkan bahwa sediaan nanoemulsi akar burdock bersifat toksik, namun terdapat faktor pengganggu yaitu ditemukan cacing parasit Taenia taeniaeformis, sehingga interpretasi hasilnya terganggu.

Subchronic Toxicity Test of Burdock Root (Arctium Lappa L.) Nanoemulsion Based on Histopathology on The Albino Rat Rattus noverticus (Wistar strain): Uji Toksisitas Subkronik Nanoemulsi Akar Burdock (Arctium Lappa L.) Berdasarkan Histopatologi pada Tikus Albino Rattus noverticus (strain Wistar) Imam Agus Faizal; Dini Puspodewi; Ajeng Puspo Aji
Indonesian Journal of Innovation Multidisipliner Research Vol. 2 No. 2 (2024): April - Juni
Publisher : Institute of Advanced Knowledge and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69693/ijim.v2i2.140

Tanaman burdock (Arctium lappa L.) merupakan tanaman herbal yang berkhasiat sebagai penambah darah (hemoglobin dan hematokrit) pada penderita anemia defisiensi besi. Sebanyak 15 ekor tikus (Rattus norvegicus) strain Wistar, jantan dan betina dibagi menjadi 3 kelompok secara random sampling yaitu Kelompok 1 Kontrol negatif (KN) tidak diberi A. lappa L. nanoemulsion. Kelompok Dosis 1 (KD1) diberi A. lappa L. nanoemulsion dengan dosis 150 mg/kgBB/hari. Kelompok Dosis 2 (KD2) diberikan A. lappa L. nanoemulsion dengan dosis 450 mg/kgBB/hari (selama 14 hari dimulai hari ke 8). Indikator penelitian ini dilihat kadar pemeriksaan biokimia kadar darah. Organ hati dan ginjal dinilai menggunakan Hematoxylin-Eosin (HE). Hasil pemeriksaan biokimia darah relatif normal. Sedangkan pewarnaan HE pada kelompok KD1 dan KD2 terjadi perubahan pada sel organ ginjal dan hati, menunjukkan bahwa sediaan nanoemulsi akar burdock bersifat toksik, namun terdapat faktor pengganggu yaitu ditemukan cacing parasit Taenia taeniaeformis, sehingga interpretasi hasilnya terganggu.

PERBANDINGAN HASIL PEMERIKSAAN KOLESTEROL TOTAL MENGGUNAKAN SAMPEL SERUM DAN PLASMA EDTA SEGERA DAN DISIMPAN SELAMA 4 JAM Luvia Isma Hanifah; Dini Puspodewi; Ira Pangesti
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.5111

Cholesterol is a lipid substance (fat component) being one among other known components, fat being a nutrient that is fulfilled by the body. The purpose of the study was to determine the comparative results of total cholesterol examination using serum and plasma EDTA immediately and stored for 4 hours. This study is analytic using cross sectional research design. The results showed in statistical tests using the Paired Sample T-Test test on serum samples immediately examined and stored for 4 hours found (p=0.015) so that the results (p>0.05) concluded there was no difference in serum samples. EDTA plasma samples immediately examined and stored for 4 hours found the results (p<0.001), comparison of total cholesterol levels using serum and EDTA plasma samples immediately examined and stored for 4 hours found (p<0.001) so that the results (p<0.05) are concluded there is a difference between total cholesterol levels in EDTA plasma immediately examined and stored for 4 hours and serum and EDTA plasma immediately examined and stored for 4 hours. Key Words : Cholesterol, Serum, EDTA Plasma, 4 Hour Delay

DIFFERENCES IN NON FASTING TRIGLICERIDE LEVELS BETWEEN 8 HOURS FASTING AND 12 HOURS FASTING Desika Dewi; Dini Puspodewi; Meka Faizal Farabi
Klinikal Sains : Jurnal Analis Kesehatan Vol. 13 No. 2 (2025): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v13i2.6604

Triglycerides are a type of blood fat that plays an important role in energy metabolism, but excessive levels can increase the risk of cardiovascular disease. The examination of triglyceride levels generally requires fasting, but the optimal duration of fasting is still debatable. This study aims to analyze the differences in triglyceride levels in non-fasting conditions, 8-hour fasting, and 12-hour fasting. This study is an analytical study with a cross sectional design involving 39 students of the Bachelor of Applied Medical Laboratory Technology Study Program at Al-Irsyad University Cilacap as samples. Blood was taken in three conditions: non-fasting, 8-hour fasting, and 12-hour fasting, then examined using the GPO-PAP colorimetric enzymatic method. The analysis showed that the average triglyceride level in the non-fasting condition was 104.59 mg/dL, 8-hour fasting was 91.90 mg/dL, and 12-hour fasting was 87.08 mg/dL. The results of the Paired Sample T-Test test showed that there was a significant difference between non-fasting conditions and 8-hour fasting (p = 0.009), as well as between non-fasting and 12-hour fasting (p < 0.001). However, there was no significant difference between 8-hour fasting and 12-hour fasting (p = 0.172). Based on these findings, it can be concluded that fasting for 8 hours is enough to significantly reduce triglyceride levels, so it can be considered as the minimum feasible duration for triglyceride. Keywords: Triglycerides, Non Fasting, 8 Hours Fasting, 12 Hours Fasting

PERBEDAAN PROFIL PROTEIN PADA TAHU SUTERA, TAHU KUNING DAN TAHU PONG METODE SDS-PAGE Raden Bagus Muhamad Tsabitul Fikriyansyah; Meka Faizal Farabi; Dini Puspodewi
Klinikal Sains : Jurnal Analis Kesehatan Vol. 13 No. 2 (2025): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar

A food made from soybeans has a nutritional content in the form of protein, namely tofu. The principle of making tofu is made from white soybeans and the process is soaked in water, ground until smooth, boiled, filtered then separate the tofu dregs and filtrate (water) and added coagulants and food additives (BTP) then molded, but there are differences in the process of making tofu. Silken tofu has a soft texture and the process of making the curd is not pressed and not separated from the liquid so it resembles jelly. Yellow tofu has a yellow color from the addition of turmeric juice solution in the manufacturing process and its dense texture. Pong tofu has a distinctive feature after the manufacturing process is fried first before being packaged. To determine the differences in protein profiles, protein profile analysis was carried out using the SDS-PAGE method, for samples of silken tofu, yellow tofu and raw pong tofu. This type of research is descriptive analytical. The first analysis of total protein content with a standard curve and using a UV-Vis spectrophotometer, then continued with protein profile analysis using an SDS-PAGE electrophoresis tool. Results of total protein content and protein profile Silken tofu has a total protein content of 0.63 µg/µL and 1 protein band. Yellow tofu has a total protein content of 0.79 µg/µL and 2 protein bands. Pong tofu has a total protein content of 1.10 µg/µL and 4 protein bands. The difference in the process of the three tofus can affect the protein content of tofu, which can increase or decrease

PENGARUH PERBANDINGAN ANTIKOAGULAN EDTA DAN VOLUME DARAH YANG TIDAK SESUAI PADA PEMERIKSAAN HEMOGLOBIN, ERITROSIT, DAN TROMBOSIT PADA PASIEN MEDICAL CHECK UP DI RS PERTAMINA CILACAP Kartika Palupi; Meka Faizal Farabi; Dini Puspodewi
Klinikal Sains : Jurnal Analis Kesehatan Vol. 13 No. 2 (2025): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar

Medical Check Up (MCU) is a procedure intended to determine the overall health condition. The results of this examination can be a reference for companies to measure the physical ability of employees to perform tasks. The purpose of this study was to determine the effect of the use of EDTA anticoagulant that was not in accordance with blood volume on the examination of hemoglobin, erythrocytes, and platelets in Medical Check Up patients at RSPC. The research sample was taken using purposive sampling technique consisting of 33 Medical Check Up patients.This study is an observational analytic study with cross sectional method. The results showed that the average hemoglobin level of 2 ml blood volume in EDTA vacutainer tubes was 15.488 g/dL while the average hemoglobin level of 3 mL blood volume in EDTA vacutainer tubes was 15.515 g/dL and the Paired Sample T-Test test showed no significant difference (p>0.05). The average erythrocyte level of 2 ml blood volume in EDTA vacutainer tubes is 5.518 million /µL, while the average erythrocyte level of 3 mL blood volume in EDTA vacutainer tubes is 5.117 million /µL and the Paired Sample T-Test test shows no significant difference (p>0.05). The average platelet level of 2 ml blood volume in EDTA vacutainer tubes is 274.576 thousand/µL while the average platelet level of 3 mL blood volume in EDTA vacutainer tubes is 267.970 thousand/µL and from the Wilcoxon Signed Rank Test which shows that there is a significant difference in platelet levels (p<0.05).

Title

Found 26 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 26 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 26 Documents
Search