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All Journal Jurnal Ilmu Pertanian Indonesia Vegetalika Journal of Degraded and Mining Lands Management Jurnal Agroekoteknologi Proceeding Seminar LPPM UMP Tahun 2014 Planta Tropika BERITA BIOLOGI Jurnal Penelitian Pertanian Terapan Panrita Abdi - Jurnal Pengabdian pada Masyarakat Kultivasi Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Agrin : Jurnal Penelitian Pertanian Jurnal Agrotek Tropika Dinamika Journal : Pengabdian Masyarakat Jurnal Abdi Insani Jurnal Puruhita
Sapto Nugroho Hadi
Agroecology Laboratory, Faculty Of Agriculture, Jenderal Soedirman University
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Social Sciences Other

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Journal : Planta Tropika

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GENETIC DIVERSITY OF POTATO BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA AND SIMPLE SEQUENCE REPEAT MARKER Hadi, Sapto Nugroho; Nurchasanah, Siti
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 8, No 1 (2020)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2020.114.54-62

Abstract

Various potato clones are cultivated by farmers in Banjarnegara and Wonosobo, Central Java, Indonesia such as MZ, NH1, NH2, Vega, Gareta, Granola, Bliss, Merah (Red Potato), Ungu (Purple Potato), Klon_17 (K17), Local Dieng, Margahayu, and X. This encourages the importance of genetic diversity and genetic similarities. This study aimed to analyze genetic diversity and genetic similarities between 13 accessions of potatoes based on seven Random Amplified Polymorphic DNA (RAPD) primers and nine pairs of Simple Sequence Repeat Marker (SSR) primers. The results showed that RAPD and SSR primers could be used to analyze genetic diversity and genetic similarities of 13 potatoes accessions from Banjarnegara and Wonosobo. The PLP value was 80.9% in the RAPD primer and 65.41% in the SSR primer. Four RAPD primers were informative based on PIC value: OPG 08, OPM 19, OPP 13, and OPX 04. Three SSR primer were informative: STM 2005, RGH- SSR 8, and StI 035. Genetic similarities presented by Phylogenetic tree analysis resulted in two main clusters. The first cluster consisted of Granola, MZ, Ungu (Purple potato), Merah (Red Potato), Local Dieng, Margahayu, Gareta, Vega, NH2, NH1, Klon_17 (K17), and Bliss. The second cluster consisted of X. Granola and MZ having a high genetic similarity with a genetic distance of 0.07 and 0.132. Meanwhile, K17 and X had a low genetic similarity with a genetic distance of 0.31 and 0.987.
Identification of Potential Biofertilizer and Bioremediator Bacteria from Upland Soil Based on 16s rDNA Sequence Analysis Hadi, Sapto Nugroho; Widiyawati, Ida; Fauzi, Ahmad; Dewi, Prita Sari; Ahadiyat, Yugi R.
PLANTA TROPIKA Vol 11, No 2 (2023)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v11i2.12744

Abstract

The long-term presence of synthetic pesticides on agricultural land can lead to a decline in soil fertility. Synthetic pesticides inhibit the activity of essential enzymes in the soil and suppress beneficial microbial populations for plants. One potential approach to mitigate the extent of contamination caused by synthetic pesticides involves the utilization of indigenous pesticide-resistant bacteria. Several upland soil bacteria from Banyumas Regency, Central Java Province, Indonesia, were successfully isolated from a previous study. The isolated bacteria have the potential to be developed as pesticide bio-remediators and biofertilizers. The bacterial isolates are expected to have characters that support plant growth through their ability to provide dissolved phosphate. However, the potential bacterial isolates need to be identified by molecular approaches. This study was conducted to identify bacterial isolates of GT2, SR1, SW1, and PA1 by 16S rDNA sequencing analysis. The results showed that isolate GT2 was placed within a group of reference strains of Bacillus proteolyticus, isolate SR1 was placed within a group of B. paramycoides, isolate SW1 was set within a group of B. albus, and isolate PA1 was placed within a group of Acidovorax delafieldii. The genetic distance of isolate GT2 and B. B. proteolyticus, isolate SR1 and B. paramycoides, isolate SW1 and B. albus were 0.0000 each, and isolate PA1 and A. delafieldii were 0.0061. 
Co-Authors ., Saparso Afandi, Dadang Agung Adi Nugroho AHADIYAT YUGI R. Ahmad Fauzi Anung Slamet Dwi Cahyani, Wilis Entis Sutisna Faozi, Khavid Fatichin, Fatichin Fauzi Ahmad Muda Giriluhita Indraswari, Giriluhita Herliana, Okti Ida Widiyawati Ida Widiyawati Ika Fitriana Dyah Ratnasari Indra Syahputra Intan Abas Nur Khairunnisa MARTIANSYAH, Irfan Mukmin, Restu Prasetya Musthafa, Muhammad Bachtiar Nani Mulyani Noviyanti, Rintis Nurchasanah, Siti Okti Herliana Okti Herliana, Okti Prita Sari Dewi PUTRANTO, Riza Arief Risa Okiana Safitri Rostaman Sari, Dini Astika Sarjiya Antonius Slamet Rohadi Suparto Syaeful Anwar Tamad, Tamad Tirta Kumala Dewi Totok Agung Dwi Haryanto TRI HARJOSO Wilis Cahyani Wilis Cahyani