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All Journal Jurnal Ilmu Pertanian Indonesia Buletin Peternakan Jurnal Veteriner Jurnal Ilmu Ternak TELKOMNIKA (Telecommunication Computing Electronics and Control) Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak Veteriner Jurnal Peternakan Indonesia Animal Production : Indonesian Journal of Animal Production Jurnal Ternak Indonesian Journal of Electrical Engineering and Computer Science Jurnal Triton Jurnal Kedokteran Hewan Jurnal Agripet Animal Production
Rudi Afnan
IPB University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary Other

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Journal : Jurnal Agripet

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Reduce Heat Stress on Broilers During Transport by Supplying Drinking Water Insan Mujahid Afnan; Niken Ulupi; Rudi Afnan
Jurnal Agripet Vol 22, No 2 (2022): Volume 22, No. 2, Oktober 2022
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v22i2.26391

Abstract

ABSTRACT. The transportation process causes heat stress in broilers. This research aims to test the effectiveness of the drinking water supply system for broilers during transport to reduce the effects of heat stress caused by the transportation process. Broilers are grouped into four treatments: morning transport with water (T1), morning transport without water (T2), afternoon transport with water (T3), and afternoon transport without water (T4). Results show that broilers consume more water (317.26 ml/bird/hour) during morning than during afternoon transport (61.53 ml/ bird/hour). However, these numbers are estimated to be lower, with water spillage contributing to the high consumption, especially during morning transport. Rectal temperature for birds is lower in T1 and T3 broilers compared to T2 and T4 broilers, although still within normal range. A decrease in hematocrit and blood glucose levels while still at normal levels is observed for all treatment groups. An increase in the ratio of heterophile and lymphocyte (HL ratio) is observed within the normal range except for T1. Percentage of body weight loss is lower on broilers with access to water: T1 (3.5%) and P3 (4.4%) compared to broilers without access to water: T2 (4.0%) and T4 (5.0%) in the same time of transport although not statistically significant (P0.05). Based on weight loss percentage, it can be concluded that water intake during transport has some effectiveness in reducing heat stress with the best results shown on broilers transported in the morning with drinking water..(Mengurangi cekaman panas pada ayam broiler saat transportasi melalui pemberian air minum)ABSTRAK. Proses transportasi menyebabkan ayam mengalami cekaman panas. Penelitian ini bertujuan untuk mengkaji efektifitas pemberian air minum selama transportasi dalam upaya mengurangi dampak cekaman panas dan meningkatkan kesejahteraan hewan dengan cara mengurangi dehidrasi dan cekaman yang disebabkan proses transportasi pada ayam broiler. Ayam dikelompokkan menjadi empat perlakuan: transportasi pagi dengan air minum (T1), transportasi pagi tanpa air minum (T2), transportasi siang dengan air minum (T3), dan transportasi pagi tanpa air minum (T4). Hasil menunjukkan ayam lebih banyak mengonsumsi air (317,26 ml/ekor/jam) pada transportasi pagi daripada transportasi siang (61,53 ml/ekor/jam). Namun, nilai ini diestimasi lebih rendah dari yang didapatkan, dengan air yang tumpah berkontribusi terhadap tingginya konsumsi air, terutama pada transportasi pagi hari. Suhu rektal lebih rendah pada broiler T1 dan T3 dibandingkan T2 and T4 namun berada dalam rentang normal. Penurunan kadar glukosa darah dan hematokrit terjadi pada seluruh perlakuan. Peningkatan rasio HL terjadi pada seluruh perlakuan kecuali T1. Persentase penurunan bobot badan lebih rendah pada ayam yang diberi air minum T1 (3,5%) dan T3 (4,4%) dibandingkan dengan ayam yang tidak diberi air minum T2 (4,0%) dan T4 (5,0%) pada waktu transportasi yang sama meskipun secara statistik tidak signifikan (P0,05). Berdasarkan perbedaan persentase penurunan bobot badan, dapat disimpulkan bahwa konsumsi air mengurangi dampak cekaman panas yang dialami ayam broiler selama transportasi dengan hasil terbaik didapatkan pada transportasi pagi dengan air minum.
Pengaruh Polimorfisme Gen Heat Shock Protein 70 (HSP70) SacII terhadap Toleransi Panas Itik Lokal Sumatera Barat Kusnadidi Subekti; Dedi Duryadi Solihin; Rudi Afnan; Asep Gunawan; Cece Sumanri
Jurnal Agripet Vol 19, No 2 (2019): Volume 19, No. 2, Oktober 2019
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v19i2.14876

Abstract

ABSTRAK. Tujuan dari penelitian ini adalah untuk mengidentifikasi pengaruh polimorfisme gen heat shock protein 70 (HSP70) terhadap sifat toleransi panas. Genotiping dilakukan pada 110 itik lokal dari 4 populasi itik Sumatera Barat (pitalah, bayang, kamang, dan payakumbuh). Enzim restriksi yang digunakan adalah SacII. Cekaman panas dilakukan selama 1 jam dengan sampel itik lokal sebanyak 24 ekor untuk menganalisa status hematologi. PCR-RFLP dan Sanger sequencing assay digunakan untuk mengidentifikasi polimorfisme. Analisis polimorfisme dilakukan dengan menggunakan Software MEGA 7 dan pengaruhnya dianalisis dengan ANOVA menggunakan software SAS 9.4. Produk amplifikasi yang dihasilkan yaitu 466 pasang basa. Lokus HSP70/SacII mengenali satu situs SNP (Single nucleotide Polymorphism) g.1702TC, menghasilkan dua alel (T dan C) dan tiga genotype (TT, TC, CC). Gen HSP70/SacII bersifat polimorfik pada semua populasi. Hasil penelitian menunjukkan bahwa alel T memiliki frekuensi yang lebih tinggi daripada alel C pada semua populasi. Analisis chi-kuadrat (2) menunjukkan bahwa semua populasi itik lokal memenuhi kaidah kesetimbangan Hardy-Weinberg. Pengaruh genotipe gen HSP70 terhadap status hematologi menunjukkan perbedaan (P0,05) terhadap nilai heterofil dan nilai H/L dengan genotipe CT menunjukkan toleransi panas yang lebih baik dibanding genotipe lainnya. Gen HSP70/SacII dapat dijadikan marka molekuler sifat toleransi panas pada itik lokal berdasarkan keragaman dan pengaruhnya terhadap status hematologi.(The effect of heat shock protein 70 (HSP70) SacII gene polymorphism on heat tolerance of West Sumatera local duck)ABSTRACT. The objective of this study was to identify the effect of HSP70/SacII gene polymorphism associated with the thermotolerance trait. Genotyping was performed on 110 local duck from the West Sumatera ducks population (pitalah, bayang, kamang, and payakumbuh). The restriction enzyme used was SacII. Heat stress was done for 1 hour with samples used as much as 24 birds to analyze hematological status. PCR-RFLP and Sanger sequencing assays were used to identify polymorphism. Analysis of polymorphism was conduct by MEGA 7 software and its effect is analyzed with ANOVA using Statistical SAS 9.4 software. The product of amplification was 466 bp. HSP70/SacII recognized one SNP g.1702TC, producing two alleles (T and C) and three genotypes (TT,TC,CC). The HSP70/SacII locus were polymorphic in all population. T allele had a higher frequency than C allele in all populations. The analysis of chi-square (2) showed that all local ducks population were fit with Hardy-Weinberg equilibrium. The effect of HSP70/SacII gene on hematological status showed differences (P0,05) of heterophil and H/L ratio with CT genotype show better heat tolerance than other genotypes. HSP70/SacII gene can be used as a marker of heat tolerance in local ducks based on polymorphism and its effect on hematological status.
Co-Authors A.S. Satyaningtijas Adam Kustiadi Nugraha Agasi, Satri Yusasra ahmad yani Anas Qurniawan Andi Yulyani Fadwiwati Any Aryani Arif Hidayad Ashari Arif Kurnia Wijayanto Asep Gunawan Aulia Fitriawati Polontalo Bahri Syamsuryadi Bayu Adhitya Nugraha Cece Sumanri Cece Sumantri Damiana Rita Ekastuti Daryatmo Daryatmo Dedi Duryadi Solihin Dewi Kartika Dhini Nova Widyasari Epi Taufik Fajar Hidayat Hariono Hariono Hariono Hariono Hidayat, Numan Idana, Danar Bias Insan Mujahid Afnan Irma Isnafia Arief Irman Hermadi Jonathan Anugrah Lase Kadarisman, Agus Kaiin, Ekayanti Mulyawati Kudang Boro Seminar Kusnadidi Subekti Luki Abdullah Mahmud, Andi Tenri Bau Astuti Mohammad Yamin Mohammad Yamin, Mohammad Niken Ulupi Nu’man Hidayat Priyambodo, Danang R. Iis Arifiantini Rita Mutia Roni Fadilah Rosikin, Rosikin Rudy Priyanto SATRIYAS ILYAS Shabrina Dyah Wibawanti Shabrina Dyah Wibawanti Sri Darwati sri murtini . Sumiarti Sumiarti Sumiati Sumiati Sumiati Sumiati T. Suryati Tarigan, Indri Nur Krisna TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Tike Sartika Tuti Suryati Wahyuni Wahyuni Wibawanti, Shabrina Dyah Yusra Fernando Zakiah Wulandari