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All Journal Pharmaciana: Jurnal Kefarmasian Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat EduChemia: Jurnal Kimia dan Pendidikan Klinikal Sains (Jurnal Analis Kesehatan) Jurnal Ilmiah Kesehatan Anakes: Jurnal Ilmiah Analis Kesehatan Jurnal Abdimas Kartika Wijayakusuma Mukhtabar: Journal of Medical Laboratory Technology
Ayi Furqon
Universitas Jenderal Achmad Yani Cimahi
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Engineering Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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STABILITAS KONSENTRASI GLUKOSA DARAH SIMPAN JANGKA PENDEK DALAM TABUNG BERTEKNOLOGI PEMISAH JELl Furqon, Ayi; Nurmukhlis, Hilman; Kasiman, Sussylawati
Pharmaciana Vol 5, No 2 (2015): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (424.448 KB) | DOI: 10.12928/pharmaciana.v5i2.2310

Abstract

Serum  glucose  level  is  screening  for  diabetic  patients.  Preanalitical  factors  such  as  sample preparation  and  handling  will  influence  the  quality  of  final  result  of  determination.  Gel  sparation technology used for faster sparation between plasma  or serum and  blood cells. This  technology was applied in clinical laboratory technology in stead of public health laboratory. Requested test of glucose level  determination  is  higher  then  other  analytes. Direct  contact  of  blood  cells  to serum  will  reduce availability  of  glucose  in  serum  due to  glycolysis  process as  well  as  without  additional  enzyme inhibitors  such as  fluoride  and  without  separating  cells  from  serum  immediately. Serum separating Tube (SST)contains gel and clot activators, it will work faster than plain tube does to become serum. Main  purpose  of  research,how  stability  of  glucose  level  between  SST  tube  and  plain  tube . Experimental steps   are preparing fresh   blood that   is  collected   from 16 voluntered   students, incorporating the glucose oxidase enzymatic assay and quality control. Test performed at immediately late 4 hours, and very late 12 haours. Statistical method for the time to time analysis, one way Anova is  used within significant level 0.05. From 16 serum (duplicates), glucose serum level was reduced early at 4 -12 hours by 8,1  mg/dl  (p-value  =  0.013) and  7,7  mg/dl  (p-value  0.012).  Percents increases  of glucose level within plain tube  4 hours by 4,75% and within 12 hours by 13,12%. The increased serum glucose  level  in SST  tube were  at  early 4 hours and 12 hoursby 4,33%  and 12,29% respectively. Conclussion, glucose serum level is in steady at room temperature until 4 hours for both SST Tube as well as plain tube.
STABILITAS KONSENTRASI GLUKOSA DARAH SIMPAN JANGKA PENDEK DALAM TABUNG BERTEKNOLOGI PEMISAH JELl Ayi Furqon; Hilman Nurmukhlis; Sussylawati Kasiman
Pharmaciana Vol 5, No 2 (2015): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (424.448 KB) | DOI: 10.12928/pharmaciana.v5i2.2310

Abstract

Serum glucose level is screening for diabetic patients. Preanalitical factors such as sample preparation and handling will influence the quality of final result of determination. Gel sparation technology used for faster sparation between plasma or serum and blood cells. This technology was applied in clinical laboratory technology in stead of public health laboratory. Requested test of glucose level determination is higher then other analytes. Direct contact of blood cells to serum will reduce availability of glucose in serum due to glycolysis process as well as without additional enzyme inhibitors such as fluoride and without separating cells from serum immediately. Serum separating Tube (SST) contains gel and clot activators, it will work faster than plain tube does to become serum. Main purpose of research, how stability of glucose level between SST tube and plain tube . Experimental steps are preparing fresh blood that is collected from 16 voluntered students, incorporating the glucose oxidase enzymatic assay and quality control. Test performed at immediately, late 4 hours, and very late 12 haours. Statistical method for the time to time analysis, one way Anova is used within significant level 0.05. From 16 serum (duplicates), glucose serum level was reduced early at 4 - 12 hours by 8,1 mg/dl (p-value = 0.013) and 7,7 mg/dl (p-value 0.012). Percents increases of glucose level within plain tube 4 hours by 4,75% and within 12 hours by 13,12%. The increased serum glucose level in SST tube were at early 4 hours and 12 hours by 4,33% and 12,29% respectively. Conclussion, glucose serum level is in steady at room temperature until 4 hours for both SST Tube as well as plain tube
HUBUNGAN LAMA KERJA DENGAN AKTIVITAS ENZIM ALKALINE PHOSPHATASE (ALP) PADA PETANI DI DAERAH LEMBANG Fini Ainun Qolbi Wasdili; Cindy Dhea Octaviani; Ayi Furqon
Klinikal Sains : Jurnal Analis Kesehatan Vol 10 No 2 (2022): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v10i2.2707

Abstract

Pestisida adalah bahan kimia yang digunakan untuk meracuni hama. Petani di Indonesia banyak yang menggunakan pestisida namun banyak pula yang tidak memahami aturan penggunaan nya. Semakin sering petani terpapar pestisida maka akan menimbulkan dampak kesehatan yaitu gangguan fungsi hati, salah satu parameter pemeriksaan fungsi hati yaitu enzim Alkaline Phosphatase (ALP). Enzim ALP adalah enzim yang diproduksi oleh sel hati dan sel pembentuk tulang. Penelitian ini bertujuan untuk mengetahui hubungan lama kerja dengan aktivitas enzim ALP pada petani di daerah Lembang. Metode penelitian yang digunakan bersifat deskriptif kemudian dilakukan analisis statistik One Way ANOVA untuk mengetahui hubungan.Pengumpulan data dilakukan melalui kuesioner, sampel berasal dari 22 Petani pada Kelompok Tani X Di Desa Cibodas Kecamatan Lembang, Kabupaten Bandung Barat, Jawa Barat. Rata-rata aktivitas ALP petani yang bekerja 1-5 tahun 57 U/I sedangkan yang lebih dari 5 tahun 35 U/I. Hasil Uji statistik One Way ANOVA diperoleh nilai signifikan 0,000 < 0,05. Kesimpulan dalam penelitian ini ada hubungan antara lama kerja dengan aktivitas ALP pada petani di Kelompok Tani X.
WEBINAR PEMULASARAN JENAZAH COVID-19 DI LUAR FASILITAS PELAYANAN KESEHATAN Wasdili, Fini Ainun Qolbi; Mahargyani, Wikan -; Romlah, Sitti; Herawati, Iis; Novilla, Arina; Furqon, Ayi; Ningrum, Nining Ratna
DHARMAKARYA: Jurnal Aplikasi Ipteks untuk Masyarakat Vol 13, No 1 (2024): Maret, 2024
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/dharmakarya.v13i1.40607

Abstract

Pandemi Covid 19 di Indonesia semakin meningkat, tenaga kesehatan, masyarakat dan pengurus Dewan Kemakmuran Mesjid (DKM) berperan dalam membantu pemulasaran jenazah. Prodi Teknologi Laboratorium Medis D-3 memfasilitasi pelaksanaan webinar pemulasaran jenazah Covid-19 untuk wilayah cimahi dan Kabupaten Bandung. Tahapan pelaksanaan terdiri dari identifikasi permasalahan pandemi Covid-19, menyusun dan mengkordinasikan kegiatan webinar, penyebaran informasi pendaftaran dan kegiatan, pelaksanaan kegiatan webinar, sesi diskusi, dan evaluasi. Tingkat pengetahuan masyarakat terhadap pemulasaran jenazah Covid-19 sebanyak 43% tidak mengetahui. Berdasarkan hasil kuesioner 96% menyatakan sangat bermanfaat dan penilaian keseluruhan kegiatan adalah 75% sangat sesuai. Di akhir acara perwakilan DKM diberikan Gown untuk membantu proses pemulasaran jenazah Covid-19.
Analysis and Optimization of Betanin Extraction from Juice of the Peel and Fleshes of Red Dragon Fruit (Hylocereus costaricencis) Kania, Prina Puspa; Furqon, Ayi
EduChemia: Jurnal Kimia dan Pendidikan Vol 9, No 1 (2024)
Publisher : Department of Chemical Education Faculty of Teacher Training and Education Universitas Su

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30870/educhemia.v9i1.23409

Abstract

Currently, health issues are no longer related to how to treat diseases but rather prioritize the prevention of various diseases by eating foods that contain high levels of nutrients, known as functional foods. Red dragon fruit contains betanin dye, which has various health benefits and can be grouped into functional foods. Some studies have been conducted to obtain betanin from red dragon fruit, but it is not yet known how much betanin content is present in the juice of the flesh and peel of red dragon fruit (Hylocereus costaricencis) and how to determine the optimum extraction conditions. This study aims to analyze the betanin content in the skin and flesh of red dragon fruit and the optimal extraction conditions so that betanin can be obtained in maximum quantities. The extraction method used was the maceration method with three solvents, namely, water, ethanol, and methanol. Analysis of the betanin content in the skin and flesh of dragon fruit was carried out using UV‒Vis spectrophotometry. The results of the study revealed that water solvents can extract betanin well. In addition, the ratios of dragon fruit to water were 2:1 (flesh and water) and 1:1 (skin and water), respectively, while the optimum extraction temperature was 4–8°C. The optimum extraction time was 24 hours. Betanin was obtained from the skin and flesh of red dragon fruit at a sample:solvent ratio of 1:1, with values of 21.71 mg/mL and 17.8 mg/mL, respectively.
Skrining Talasemia Pada Mahasiswa TLM (D3) Fakultas Ilmu Dan Teknologi Kesehatan Universitas Jenderal Achmad Yani Cimahi: Skrining Talasemia Pada Mahasiswa TLM (D3) Fakultas Ilmu Dan Teknologi Kesehatan Universitas Jenderal Achmad Yani Cimahi Novilla, Arina; Clarisa, ⁠Tiara Nur; Furqon, Ayi; Romlah, Sitti
Jurnal Ilmiah Kesehatan Vol 16 No 2 (2024): Jurnal Ilmiah Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/jik.v16i2.2327

Abstract

Menurut data Organisasi Kesehatan Dunia (WHO) pada tahun 2021, diperkirakan bahwa sekitar 20% dari seluruh populasi dunia menderita talasemia beta mayor. Di Indonesia, prevalensi talasemia berkisar antara 6-10%, dan provinsi Jawa Barat mempunyai kasus talasemia mayor tertinggi. Talasemia mayor terjadi akibat perkawinan antara individu yang membawa gen talasemia atau menderita talasemia minor, yang seringkali tanpa gejala, sehingga deteksi dini sangat penting untuk mencegah kelahiran anak dengan talasemia mayor.Penelitian ini bertujuan untuk mengetahui skrining talasemia menggunakan uji fragilitas osmotic (OTOF/One Tube Osmotic Fragility), hasil positif dilanjutkan dengan penentuan nilai indeks eritrosit, lalu dilanjutkan dengan penentuan Indeks Mentzer. Penelitian ini menggunakan metode deskriptif, subjek penelitian adalah mahasiswa TLM (D3) di Fakultas Ilmu dan Teknologi Kesehatan Universitas Jenderal Achmad Yani, Cimahi. Berdasarkan hasil penelitian dari 155 mahasiswa TLM (D3), hasil skrining talasemia minor menunjukkan bahwa 10 orang (6%) menunjukkan hasil OTOF positif. Hasil yang positif dilanjutkan pemeriksaan darah lengkap untuk menentukan Indeks Eritrosit, sebanyak 8 orang (80%) memiliki MCV <80 fL dan MCH <27 pg. Hasil MCV dan MCH rendah tersebut kemudian ditentukan nilai Indeks Mentzer dan didapatkan sebanyak 2 orang (25%) memiliki Indeks Mentzer <13. Dari penelitian ini disimpulkan bahwa terdapat 2 orang (1%) yang terindikasi menderita talasemia beta minor, dan disarankan selanjutnya untuk dilakukan pemeriksaan elektroforesis hemoglobin.
The Effect of Stress Levels on Blood Glucose Levels in Medical Laboratory Technology Final-Year Students Nursidika, Perdina; Astari, Risti Maulani; Furqon, Ayi
Mukhtabar Journal of Medical Laboratory Technology Vol 2 No 2 (2024): Mukhtabar: Journal of Medical Laboratory Technology (October 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v2i2.684

Abstract

Background & Objective: Final-year students are a group that experiences stress due to academic life, responsibilities, and demands from the academic world, such as completing theses, fieldwork, and other academic burden. In addition, the current pandemic may cause stress related to communication and daily life changes. Stress increases the release of epinephrine, catecholamine, glucagon, glucocorticoids, beta-endorphin, and growth hormone, which causes excessive cortisol production, increasing blood sugar levels. This study aimed to determine the relationship between stress levels and blood sugar levels in undergraduate students in medical laboratory technology. Method: This research method used a cross-sectional approach, with 35 samples that fit the inclusion criteria. The stress measuring tool used was the Depression Anxiety Stress Scale (DASS) questionnaire. Blood glucose examination using the Glucose Oxidase Peroxidase Aminoantypirin method. The analysis used in this study is the correlation test using the Chi-square test Result: Stress levels in students show normal stress at as much as 11% mild stress at 26% moderate stress at 46%, and severe stress at as much as 17%. The results of bivariate analysis between stress levels and blood glucose levels were p=0.012 (p<0.05). Conclusion: there is a significant relationship between stress levels and blood glucose levels in final-year students of D4 Medical Laboratory Technology at Jenderal Achmad Yani University, Cimahi. The results of this study became one of the prediabetes screening data. The need for stress management in students can be one of the factors to avoid the risk of diabetes.
Optimasi Metode Pemeriksaan HbA1c Secara Elektrokimia Berbasis Screen Printed Carbon Electrode Furqon, Ayi; Kania, Prina Puspa
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 2 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i2.2314

Abstract

The quantification of HbA1c as an analyte used to monitor the success of diabetes treatment is generally examined in a laboratory with sophisticated technological equipment and professional staff. Point of care testing (POCT) is generally designed for practical examination at the patient's side. POCT HbA1c uses a screen-printed electrode (SPCE) reagent strip labeled with aptamer molecular imprinted polymer (MIP), boronate, or anti-HbA1c antibody and then quantified electrochemically. The linearity of the measurement works at a normal detection concentration of 5.5-8.5% but has not been tested at high critical concentrations. The purpose of this study was to optimize the electrochemical quantification method of HbA1c. The research procedure, SPCE was first activated with 0.1 M NaOH by cyclic voltammetry. Aptamer labeling (Fe3O4@MIPHbA1c) was carried out using the chronoamperometric technique and quantification was determined by the differential pulse voltammetry (DPV) technique. HbA1c stock of 6% and 10% in PBS pH 7.4 was used as normal and pathological concentration solution in accuracy and precision study (target quality CV <3%). The result, the average normal concentration of HbA1c was 5.9% ± 0.1%, CV=1.69% and pathological concentration was 10.4% ± 0.21%, CV=1.92%. The linearity test result of measurement ranged from 2%-15% R2=0.987. In conclusion, MIPHbA1c-activated SPCE showed optimal condition in HbA1c measurement in the normal and pathological range. Keywords: SPCE, HbA1c POCT, HbA1c Magnetit, HbA1c Aptamer
Optimasi Metode Pemeriksaan HbA1c Secara Elektrokimia Berbasis Screen Printed Carbon Electrode Furqon, Ayi; Kania, Prina Puspa
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 2 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i2.2314

Abstract

The quantification of HbA1c as an analyte used to monitor the success of diabetes treatment is generally examined in a laboratory with sophisticated technological equipment and professional staff. Point of care testing (POCT) is generally designed for practical examination at the patient's side. POCT HbA1c uses a screen-printed electrode (SPCE) reagent strip labeled with aptamer molecular imprinted polymer (MIP), boronate, or anti-HbA1c antibody and then quantified electrochemically. The linearity of the measurement works at a normal detection concentration of 5.5-8.5% but has not been tested at high critical concentrations. The purpose of this study was to optimize the electrochemical quantification method of HbA1c. The research procedure, SPCE was first activated with 0.1 M NaOH by cyclic voltammetry. Aptamer labeling (Fe3O4@MIPHbA1c) was carried out using the chronoamperometric technique and quantification was determined by the differential pulse voltammetry (DPV) technique. HbA1c stock of 6% and 10% in PBS pH 7.4 was used as normal and pathological concentration solution in accuracy and precision study (target quality CV <3%). The result, the average normal concentration of HbA1c was 5.9% ± 0.1%, CV=1.69% and pathological concentration was 10.4% ± 0.21%, CV=1.92%. The linearity test result of measurement ranged from 2%-15% R2=0.987. In conclusion, MIPHbA1c-activated SPCE showed optimal condition in HbA1c measurement in the normal and pathological range. Keywords: SPCE, HbA1c POCT, HbA1c Magnetit, HbA1c Aptamer
Skrining Talasemia Pada Mahasiswa TLM (D3) Fakultas Ilmu Dan Teknologi Kesehatan Universitas Jenderal Achmad Yani Cimahi: Skrining Talasemia Pada Mahasiswa TLM (D3) Fakultas Ilmu Dan Teknologi Kesehatan Universitas Jenderal Achmad Yani Cimahi Novilla, Arina; Clarisa, ⁠Tiara Nur; Furqon, Ayi; Romlah, Sitti
Jurnal Ilmiah Kesehatan Vol 16 No 2 (2024): Jurnal Ilmiah Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/jik.v16i2.2327

Abstract

Menurut data Organisasi Kesehatan Dunia (WHO) pada tahun 2021, diperkirakan bahwa sekitar 20% dari seluruh populasi dunia menderita talasemia beta mayor. Di Indonesia, prevalensi talasemia berkisar antara 6-10%, dan provinsi Jawa Barat mempunyai kasus talasemia mayor tertinggi. Talasemia mayor terjadi akibat perkawinan antara individu yang membawa gen talasemia atau menderita talasemia minor, yang seringkali tanpa gejala, sehingga deteksi dini sangat penting untuk mencegah kelahiran anak dengan talasemia mayor.Penelitian ini bertujuan untuk mengetahui skrining talasemia menggunakan uji fragilitas osmotic (OTOF/One Tube Osmotic Fragility), hasil positif dilanjutkan dengan penentuan nilai indeks eritrosit, lalu dilanjutkan dengan penentuan Indeks Mentzer. Penelitian ini menggunakan metode deskriptif, subjek penelitian adalah mahasiswa TLM (D3) di Fakultas Ilmu dan Teknologi Kesehatan Universitas Jenderal Achmad Yani, Cimahi. Berdasarkan hasil penelitian dari 155 mahasiswa TLM (D3), hasil skrining talasemia minor menunjukkan bahwa 10 orang (6%) menunjukkan hasil OTOF positif. Hasil yang positif dilanjutkan pemeriksaan darah lengkap untuk menentukan Indeks Eritrosit, sebanyak 8 orang (80%) memiliki MCV <80 fL dan MCH <27 pg. Hasil MCV dan MCH rendah tersebut kemudian ditentukan nilai Indeks Mentzer dan didapatkan sebanyak 2 orang (25%) memiliki Indeks Mentzer <13. Dari penelitian ini disimpulkan bahwa terdapat 2 orang (1%) yang terindikasi menderita talasemia beta minor, dan disarankan selanjutnya untuk dilakukan pemeriksaan elektroforesis hemoglobin.
Co-Authors Astari, Risti Maulani Cindy Dhea Octaviani Clarisa, ⁠Tiara Nur Friliansari, Lilis Puspa Gina Khairinisa Gunawan, Taufik Hilman Nurmukhlis Hilman Nurmukhlis, Hilman IIS HERAWATI Kania, Prina Puspa Kania, Prina Puspa Mahargyani, Wikan Naully, Patricia Gita Ningrum, Nining Ratna Novilla, Arina Perdina Nursidika Rachmawati, Firdha Rukmana SITTI ROMLAH Sussylawati Kasiman Sussylawati Kasiman, Sussylawati Wasdili, Fini Ainun Qolbi