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Pemsi M. Wowor, Pemsi M.
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Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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UJI TOKSISITAS AKUT EKSTRAK SPONS LAUT (CALLYSPONGIA AERIZUSA) TERHADAP LARVA ARTEMIA SALINA LEACH DENGAN METODE BRINE SHRIMP LETHALITY TEST Sondakh, Refrando M.; Posangi, Jimmy; Wowor, Pemsi M.
eBiomedik Vol 5, No 2 (2017): eBiomedik
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/ebm.5.2.2017.18312

Abstract

Abstract: Indonesia has a great potential of marine biological resources. One of the coral reef ecosystems is marine sponge Callyspongia aerizusa that contains compounds of steroid, alkaloid, flavonoid, and terpenoid. In order to develop those substances as components of drugs, it is necessary to perform initial screening of its acute toxicity potential. This study was aimed to obtain the acute toxicity potential of marine sponge extract using Brine Shrimp Lethality Test (BSLT). This was an experimental study using the posttest only control group design. Samples were 180 larvae of Artemia salina Leach divided into 6 groups of 10 larvae. Each group was tested with three replications. The marine sponge extract final concentrations in the media which contained larvae consecutively in groups 1, 2, 3, 4, 5, and 6 were 1000 ?g/ml, 500 ?g/ml, 250 ?g/ml, 100 ?g/ml, 50 ?g/ml, and 0 ?g/ml as the negative control. The observation was focused to larvae that died 24 hours after the administration of the extract. LC 50 value of the marine sponge extract was analyzed by using probit analysis with SPSS 16.0. The results indicated that the extract final concentrations that killed the larvae were 1000 ?g/ml, 500 ?g/ml, and 250 ?g/ml. The result of probit analysis indicated that LC 50 value of marine sponge extract was 992.468 ?g/mL. Conclusion: The marine sponge extract had acute toxicity potential against Artemia salina Leach larvae according to BSLT with a LC 50 value <1000 ?g/Ml.Keywords: Acute toxicity test, BSLT, LC 50, Callyspongia aerizusa marine sponge Abstrak: Indonesia memiliki potensi sumber daya alam hayati laut yang besar. Salah satu ekosistem terumbu karang ialah spons laut Callyspongia sp. yang mengandung senyawa steroid, alkaloid, flavonoid, dan terpenoid. Diperlukan skrining awal mengenai potensi toksisitas akut untuk pengembangan bahan baku obat. Penelitian ini bertujuan untuk mengetahui potensi toksisitas akut ekstrak spons Callyspongia sp. menggunakan Brine Shrimp Lethality Test (BSLT). Jenis penelitian ialah eksperimental dengan posttest-only control group design. Hewan uji ialah 180 ekor larva Artemia salina Leach yang dibagi dalam 6 kelompok, masing-masing terdiri dari 10 ekor. Tiap kelompok dilakukan pengulangan percobaan 3 kali. Ekstrak spons laut diberikan dalam media yang berisi larva. Konsentrasi akhir ekstrak dalam media yang berisi larva berturut-turut dalam kelompok 1, 2, 3, 4, 5, dan 6 ialah 1000 ?g/ml, 500 ?g/ml, 250 ?g/ml, 100 ?g/ml, 50 ?g/ml dan 0 ?g/ml sebagai kontrol negatif. Pengamatan dilakukan terhadap larva yang mati 24 jam setelah pemberian bahan uji. LC 50 ekstrak spons laut ditentukan dengan analisis probit menggunakan SPSS 16.0 for windows. Hasil penelitian menunjukkan beban konsentrasi ekstrak dalam media dapat membunuh larva secara berturut-turut dengan konsentrasi 1000 ?g/ml, 500 ?g/ml, dan 250 ?g/ml. Hasil analisis probit menunjukkan nilai LC 50 dari ekstrak spons laut ialah 992,468 ?g/mL. Simpulan: Ekstrak spons laut menunjukkan adanya potensi toksisitas akut terhadap larva Artemia salina Leach menggunakan BSLT dengan nilai LC50 <1000 ?g/mL.Kata kunci: toksisitas, BSLT, spons laut Callyspongia aerizusa
Uji Efek Daya Hambat Jamur Endofit Rimpang Jahe (Zingiber officinale Rosc) terhadap Bakteri Staphylococcus aureus dan Escherichia coli Abdul, Jihan A.; Posangi, Jimmy; Wowor, Pemsi M.; Bara, Robert A.
Jurnal Biomedik : JBM Vol 12, No 2 (2020): JURNAL BIOMEDIK : JBM
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.12.2.2020.29163

Abstract

Abstract: Ginger rhizome contains antimicrobial compounds of phenol group, flavonoids, terpenoids, and aetheric oil. Endophytic fungi are commonly found in ginger plant Zingiber officinale Rosc. which is widely available in Indonesia. There is a symbiosis between these endophytic fungi and its host plants in a form of genetic material transform, thus these fungi could produce the same active compounds as the host does, or at least involved in the host’s biosynthetic pathway. This study was aimed to determine the inhibitory effect of endophytic fungi isolated from ginger (Zingiber officinale Rosc) against clinical isolates Staphylococcus aureus and Escherichia coli. The endophytic fungi were isolated and their activities were screened. Initial screening of 6 isolates showed that only 3 isolates had relatively strong inhibitory effect against the bacteria. Therefore, they were cultured again, and their extracts were tested against the same types of bacteria. The mean diameters of the inhibition zones against S. aureus were, as follows: 18.7 mm (JJR extract), 11 mm (JJA 1.3 extract), and 17.3 mm (JJA 2.1 extract). Moreover, the mean diameters of the inhibition zone against E. coli were, as follows: 18 mm (JJR extract), 17.3 mm (JJA 1.3 extract), and 23.3 mm (JJA 2.1 extract). In conclusion, endophytic fungi isolated from Zingiber officinale Rosc. had inhibitory effect against S. aureus and E. coli. The inhibitory effect against E. coli was stronger than against S. aureus.Keywords: Zingiber officinale Rosc, endophytic fungi, E. coli, S. aureus Abstrak: Rimpang jahe mengandung senyawa antimikroba fenol, flavonoid, terpenoid, dan minyak atsiri. Jamur endofit sering ditemukan pada tumbuhan jahe Zingiber officinale Rosc, yang tersebar di Indonesia. Terdapat simbiosis antara jamur endofit dan tanaman inang dalam bentuk transformasi materi genetik sehingga jamur tersebut dapat menghasilkan senyawa aktif yang sama, atau setidaknya terlibat dalam jalur biosintesis inang. Penelitian ini bertujuan untuk mengetahui daya hambat jamur endofit yang terdapat pada jahe (Zingiber officinale Rosc) terhadap bakteri Staphylococcus aureus dan Escherichia coli. Jamur endofit diisolasi dan diskrining aktivitasnya. Hasil skrining awal dari 6 isolat yang diperoleh memperlihatkan hanya 3 isolat jamur endofit memiliki daya hambat yang relatif kuat terhadap bakteri uji. Ketiga isolat tersebut dikultur kembali dan ekstraknya diuji aktivitas dengan bakteri yang sama. Hasil pengukuran zona hambat 3 isolat jamur ialah sebagai berikut: pada bakteri Staphylococcus aureus didapatkan jamur JJR 18,7 mm, jamur JJA 1.3 11 mm, dan JJA 2.1 17,3 mm; pada bakteri Escherichia coli didapatkan jamur JJR 18 mm, JJA 1.3 17,3 mm, dan JJA 2.1 23,3 mm. Simpulan penelitian ini ialah jamur endofit isolat dari Zingiber officinale Rosc. memiliki aktivitas antibakteri terhadap S. aureus dan E. coli. Daya hambat jamur endofit lebih besar terhadap E. coli dibandingkan S. aureus.Kata kunci: Zingiber officinale Rosc, jamur endofitik, E. coli, S. aureus
Uji Efek Antibakteri Chromodoris annae terhadap Bakteri Staphylococcus aureus dan Escherichia coli Faidiban, Aqueline N.; Posangi, Jimmy; Wowor, Pemsi M.; Bara, Robert A.
Medical Scope Journal Vol 1, No 2 (2020): Medical Scope Journal
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/msj.1.2.2020.27847

Abstract

Abstract: This study was aimed to evaluate the antibacterial effect of Chromodoris annae taken from Bunaken waters. This was an experimental study. The antibacterial effect was tested by using the Kirby-Bauer method. Chromodoris annae extract was made by maceration using 95% ethanol and was tested to Staphylococcus aureus and Escherichia coli bacteria. Ciprofloxacin was used as the positive control and aquadest as the negative control. The results showed that the mean inhibition zone diameter of Chromodoris annae extract to Staphylococcus aureus was 22.3 mm meanwhile of ciprofloxacin was 34.7 mm. The mean inhibition zone diameter of Chromodoris annae extract to Escherichia coli was 23.0 mm meanwhile of ciprofloxacin was 40.3 mm. Moreover, aquadest showed no inhibition zone. In conclusion, Chromodoris annae had very strong antibacterial effect to the growths of Staphylococcus aureus and Escherichia coli.Keywords: Chromodoris annae, Staphlococcus aureus, Escherichia coli Abstrak: Penelitian ini bertujuan untuk mengevaluasi efek antibakteri dari Chromodoris annae yang diambil dari perairan Bunaken. Jenis penelitian ialah eksperimental. Pengujian aktivitas antibakteri menggunakan metode Kirby-Bauer. Ekstrak Chromodoris annae dibuat dengan cara maserasi menggunakan pelarut etanol 95% dan diujikan aktivitas antibakteri terhadap bakteri Staphylococcus aureus dan Escherichia coli. Sebagai kontrol positif digunakan antibiotik ciprofloxacin dan sebagai kontrol negatif digunakan akuades. Hasil penelitian memperlihatkan rerata zona hambat ekstrak Chromodoris annae terhadap Staphylococcus aureus sebesar 22,3 mm sedangkan zona hambat ciprofloxacin sebesar 34,7 mm. Rerata zona hambat ekstrak Chromodoris annae terhadap Escherichia coli sebesar 23,0 mm sedangkan zona hambat ciprofloxacin sebesar 40,3 mm. Akuades tidak memperlihatkan adanya zona hambat. Simpulan penelitian ini ialah Chromodoris annae memiliki efek antibakteri kategori sangat kuat terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli.Kata kunci: Chromodoris annae, Staphylococcus aureus, Escherichia coli
Co-Authors Abaa, Yoel P. Abdul, Jihan A. Adriansyah L. Putra, Adriansyah L. Bernat S. P. Hutagalung Christi Mambo Christy Mambo, Christy Christy N. Mintjelungan Dendhana, Didiet S. Edward Nangoy, Edward Faidiban, Aqueline N. Hedison Polii Henoch Awaloei Herlina I. S. Wungouw Jimmy Posangi Juliatri . Kartika Pangalila, Kartika Kipimbob, Eflentina Krista V. Siagian Lambiju, Eskha M. Mambo, Christy D. Mario S. Howarto, Mario S. Michael A. Leman Miranda A. J. Makalew, Miranda A. J. Rawung, Israel Rengkuan, Raissa Y.E. Robert A. Bara Rumampuk, Yuki B.J. Sibilang, Annabelle A. G. C. Sondakh, Refrando M. Supit, Aurelia S.R. Titi L. Faraknimella, Titi L. Trigisa Lasabuda, Trigisa Tumbel, Lisa K. Tuna, Ismiranti D.A. Watuseke, Anggara E. Wenda, Yaromis Wotulo, Febrina G. Yanti M. Mewo Zega, Victorson L.