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Journal : Jurnal Bios Logos

Optimasi Konsentrasi DNA dan MgCl2 pada Reaksi Polymerase Chain Reaction-Random Amplified Polymorphic DNA untuk Analisis Keragaman Genetik Tanaman Faloak (Sterculia quadrifida R.Br) (Optimization of DNA and MgCl2 Concentrations in Polymerase Chain Reacti Uslan, Uslan; Pharmawati, Made
JURNAL BIOS LOGOS Vol 5, No 1 (2015): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.5.1.2015.9316

Abstract

Abstrak Faloak  merupakan tanaman yang tumbuh di lahan kritis. Sebagai upaya mendukung pemuliaan dan konservasi tanaman faloak diperlukan informasi keragaman genetiknya. Salah satu metode analisis keragaman genetik adalah menggunakan penanda DNA yang berbasis PCR. Untuk itu diperlukan kondisi PCR (Polymerase Chain Reaction) yang tepat sehingga diperoleh hasil yang dapat dianalisis lebih lanjut. Penelitian ini bertujuan menentukan kondisi optimum PCR-RAPD (Polymerase Chain Reaction-Random Amplified Polymorphic DNA) tanaman faloak. Ekstraksi DNA dilakukan dengan metode CTAB. Optimasi dilakukan dengan menggunakan beberapa konsentrasi DNA cetakan dan MgCl2. Kondisi optimum PCR-RAPD tanaman faloak yang menghasilkan pita produk PCR yang jelas diperoleh  menggunakan 50 ng/ul DNA, 3 mM MgCl2 serta jumlah siklus termal 45 x. Kata kunci : PCR-RAPD, optimasi, tanaman faloak Abstract Faloak is a plant that grows on critical lands. In an effort to support breeding and conservation of faloak, information about its genetic diversity is required. One of the methods of genetic diversity analysis is using PCR-based DNA markers. For that purpose, proper PCR conditions is needed in order to obtain results that can be further analyzed. This study aimed to determine the optimum conditions for PCR-RAPD of faloak plants. DNA extraction was conducted using CTAB. Optimization was done by using several concentrations of DNA templates and MgCl2. The optimum conditions of PCR-RAPD of faloak plants that produce clear band of PCR products were obtained using 50 ng/ ul DNA, 3 mM MgCl2 and 45x thermal cycles Keywords : PCR-RAPD, optimization, faloak plant
Co-Authors Abdul Syahril Muh Abuk, Maria Getrudis Ahmad ahmad yani Ahmad, Rizqy Amelia Ramadhaniyah Aiman, Ummu Aini, Nur Qurotul Ambu, Valentina Inya Ari Setiawan Asmiati Asmiati Azizah, Wiwil Binti Abdullah, Norazilawati Buchari, Abu Saman Djahamouw, Julaiha Endang Triwahyuni Ernawati Ernawati fatimah Fatimah Fauzana, Nana Gigin Ginanjar Handayani, Sri Harun, Makmur Haryanti, Yulia Hasyda, Suryadin Helmia Tasti Adri Helzi, Helzi Hoar, Febriana Holbala, Dervino I. P. Honest Ummi Kaltsum Huki, Riski Dan. A. Rame Huky, Weldiana Rame Ihwan Ihwan Ihwan Imam Mujahid Irma Inesia Sri Utami Julhidayat Muhsam Kase, Monica Lakapu, Abner Lamahala, Muhaimin H Lamen , Sumarni Leki, Martha Bano LETASADO, MUHAMAD RUSADI Linome, Bilam S Linome, Bilam S. M. Saleh, Mega MADE PHARMAWATI Mahfud Mahfud Mahmud, Nur Robiah Adawiyah Marsum Marsum Maryam Nurlaila, Maryam Masneno, Jamila Amir Meilani, Dian Minsih Missa, Irfan Muhammad, Nur'aini Nahak, Engly M. J. Nahak, Maria Devawanti Fontes Nasir, Gamar Abdul Nitano, Adelina Nurdiyah Lestari NURIYAH Nurjannah Nurjannah Nurlailah Parera, Adelviana Raisia, Alifah Ramadiansyah Ratnasari Diah Utami Rusnilawati Saleh, Mega M Selan, Debora Silfeto, Nofianti Siti Marpuah, Siti Siti Muthmainnah Yusuf Sose, Maria Fenisiana Suhada, Farhan Sya, Mega Febriani Tadzkiroh, Ummi Tamonob, Trivena R.M. Tanalinal Khasna, Fenny Tanau, Vebi Elisabet Tanehe, Tresly Lilan Taneo, Noniansi T Tapotubun, Alfonsina M. Taruddin, Taruddin Tausbele, Martensi B Trisnowati Josiah Yenni Yenni Zahra Khusnul Lathifah