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Telaah Literatur Potensi Lutein dari Bunga Marigold Lokal sebagai Suplemen Kesehatan Jovine Marcella Kurniawan; Melisa Megawati Yusuf; Heriyanto Heriyanto; Tatas Hardo Panintingjati Brotosudarmo
Media Penelitian dan Pengembangan Kesehatan Vol 30 No 2 (2020)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/mpk.v30i2.2874

Abstract Lutein is a type of xanthophyll that accumulated predominantly in eye macula, and is distributed in several parts of the human body, such as skin and brain. Lutein accumulation in the human body is associated with the health benefits of lutein, including being an antioxidant and protecting, the eyes and skin from exposure to blue light, and playing an important role to maintain human cognitive health. Lutein cannot be synthesized by human, so consumption of lutein can be obtained from foods such as fruits and green vegetables, as well as from supplements. The purpose of writing this article was to increase awareness of the importance of adequate lutein intake early in the body, as well as increased research interest in exploring local resources as a potential source of lutein, the Mega Orange marigold from Bali as a raw material for health supplements. The potential lutein article as health supplement was prepared by reviewing articles from international journals and followed by testing the composition of carotenoids in Mega Orange marigolds and a health supplement product by HPLC analysis. Various studies and article publications regarding the health benefits of lutein have been found in the last decade. There are at least two main benefits of lutein, namely as an antioxidant and to maintain the health of organs such as the eyes, brain and skin. Meanwhile, the local marigold flower variety of Mega Orange contains carotenoids lutein and zeaxanthin up to 13.88 mg/g dry weight, and this result is in the range of lutein concentrations in marigold flowers that grow in Thailand. Mega Orange marigold is a potential source of lutein that can be used as raw materials for health supplement products. The importance of lutein intake for the human body makes the use of lutein from local marigolds in the manufacture of health supplements needs to be optimized and utilized. Abstrak Lutein termasuk jenis xantofil yang terakumulasi secara dominan pada makula, serta terdistribusi pada beberapa bagian tubuh manusia seperti kulit dan otak. Akumulasi lutein pada tubuh manusia dikaitkan dengan manfaat kesehatan lutein, diantaranya sebagai antioksidan serta melindungi mata dan kulit dari paparan sinar biru, dan memainkan peranan penting untuk menjaga kesehatan kognitif manusia. Lutein tidak dapat disintesis oleh manusia, sehingga konsumsi lutein dapat diperoleh dari makanan seperti buah dan sayuran hijau, serta dari suplemen. Tujuan penulisan artikel ini adalah untuk meningkatkan kesadaran akan pentingnya kecukupan asupan lutein sejak dini dalam tubuh, serta menambah minat penelitian akan eksplorasi sumber daya lokal yang sebagai sumber potensial lutein yaitu bunga marigold Mega Orange dari Pulau Bali sebagai bahan baku suplemen kesehatan. Artikel potensi lutein sebagai suplemen kesehatan ini disusun dengan menelaah artikel-artikel pada jurnal internasional dan diikuti dengan pengujian komposisi karotenoid pada bunga marigold Mega Orange dan satu produk suplemen kesehatan dengan analisis Kromatografi Cair Kinerja Tinggi (KCKT). Berbagai penelitian dan publikasi artikel mengenai manfaat kesehatan lutein telah banyak ditemukan dalam dekade terakhir. Setidaknya terdapat dua manfaat utama lutein, yaitu sebagai antioksidan dan untuk menjaga kesehatan organ tubuh seperti mata, kulit, dan otak. Adapun, bunga marigold lokal varietas Mega Orange mengandung karotenoid lutein dan zeaksantin hingga 13,88 mg/g berat kering, dan hasil ini berada pada rentang konsentrasi lutein dalam bunga marigold yang tumbuh di Thailand. Bunga marigold Mega Orange merupakan sumber potensial lutein yang dapat digunakan sebagai bahan baku produk suplemen kesehatan. Pentingnya asupan lutein bagi tubuh manusia menjadikan pemanfaatan lutein dari bunga marigold lokal dalam pembuatan suplemen kesehatan perlu dioptimalkan dan dimanfaatkan.

Multispectral Imaging and Convolutional Neural Network for Photosynthetic Pigments Prediction Kestrilia Prilianti; Ivan C. Onggara; Marcelinus A.S. Adhiwibawa; Tatas H.P. Brotosudarmo; Syaiful Anam; Agus Suryanto
Proceeding of the Electrical Engineering Computer Science and Informatics Vol 5: EECSI 2018
Publisher : IAES Indonesia Section

The evaluation of photosynthetic pigments composition is an essential task in agricultural studies. This is due to the fact that pigments composition could well represent the plant characteristics such as age and varieties. It could also describe the plant conditions, for example, nutrient deficiency, senescence, and responses under stress. Pigment role as light absorber makes it visually colorful. This colorful appearance provides benefits to the researcher on conducting a nondestructive analysis through a plant color digital image. In this research, a multispectral digital image was used to analyze three main photosynthetic pigments, i.e., chlorophyll, carotenoid, and anthocyanin in a plant leaf. Moreover, Convolutional Neural Network (CNN) model was developed to deliver a real-time analysis system. Input of the system is a plant leaf multispectral digital image, and the output is a content prediction of the pigments. It is proven that the CNN model could well recognize the relationship pattern between leaf digital image and pigments content. The best CNN architecture was found on ShallowNet model using Adaptive Moment Estimation (Adam) optimizer, batch size 30 and trained with 15 epoch. It performs satisfying prediction with MSE 0.0037 for in sample and 0.0060 for out sample prediction (actual data range -0.1 up to 2.2).

AKTIVITAS TERMOPROTEKSI DAN FOTOPROTEKSI EKSTRAK KASAR KAROTENOID MESOKARP KELAPA SAWIT (Elaeis guineensis Jacq.) TERHADAP KESTABILAN KLOROFIL-A) Dece Elisabeth Sahertian; Indriatmoko Indriatmoko; Leenawaty Limantara; Tatas Hardo Panintingjati Brotosudarmo
Al-Kauniyah: Jurnal Biologi Vol 12, No 1 (2019): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

AbstrakKelapa sawit (Elaeis guineensis Jacq.) merupakan tanaman yang mengandung karotenoid tinggi pada mesokarp yang merupakan bagian dari buah. Cahaya dan suhu dalam proses fotosintesis memberi pengaruh bagi kestabilan dan ketidakstabilan karotenoid dan klorofil. Penelitian ini bertujuan untuk mengetahui aktivitas fotostabilitas dan termostabilitas ekstrak karotenoid mesokarp terhadap klorofil-a yang diiradiasi dengan lampu volpi (intralux 4100) daylight pada intensitas cahaya 31960 lux, 47040 lux dan 76640 lux dalam seri waktu penyinaran 0, 5, 10, 15, 20, 25, dan 30 menit serta dipanaskan pada suhu 25, 50, 65, dan 90 °C dengan seri waktu pemanasan 0, 1, 2, 3, 6, 9, dan 24 jam. Pengukuran spektrum serapan tiap perlakuan diukur menggunakan Spektrofotometer UV-Tampak pada panjang gelombang 300–800 nm. Hasil penelitian menunjukkan bahwa karotenoid tidak melakukan fungsi proteksi dengan baik pada perlakuan pemanasan 65 °C dan 90 °C selama 24 jam, serta perlakuan iradiasi hingga 30 menit pada ketiga intensitas cahaya. Kesimpulan dari penelitian ini adalah klorofil-a murni lebih stabil pada perlakuan pemanasan dan iradiasi. Kemampuan proteksi karotenoid terhadap kestabilan klorofil-a yaitu pada suhu di bawah 50 °C dan pada intensitas cahaya di bawah 31960 lux.Abstract Oil palm (Elaeis guineensis Jacq.) is a plant that contains high carotenoids in its mesocarp which is part of the fruit. Light and temperature in photosynthesis may affect the stability and instability of carotenoid and chlorophyll. The aim of this research was to examine the thermostability and photostability activities of the carotenoids in mesocarp extract on chlorophyll which were irradiated by Volpi lamp (4100 intralux) daylight at light intensity of 31960 lux, 47040 lux and 76640 lux in series of time radiation 0, 5, 10, 15, 20, 25, and 30 minutes and heated at 25, 50, 65, and 90°C with series of heating time 0, 1, 2, 3, 6, 9, and 24 hours. Measurement of the absorption spectrum of each treatment was measured using a UV-Vis Spectrophotometer at a wavelength range of 300–800 nm. The results showed the protective function of carotenoids did not perform well at the heating treatments of 65°C and 90°C in 24 hours and at the irradiation for 30 minutes in all the light intensities. In conclusion, pure chlorophyll-a is more stable in heating and irradiation treatments. Carotenoid protection ability against chlorophyll-a is at temperatures below 50 °C and at a light intensity below 31960 lux.

STUDY ON THE STRUCTURAL BASIS OF PERIPHERAL LIGHT HARVESTING COMPLEXES (LH2) IN PURPLE NON-SULPHUR PHOTOSYNTHETIC BACTERIA Tatas H.P. Brotosudarmo; Richard J. Cogdell
Indonesian Journal of Chemistry Vol 10, No 3 (2010)
Publisher : Universitas Gadjah Mada

Photosynthesis provides an example of a natural process that has been optimized during evolution to harness solar energy efficiently and safely, and finally to use it to produce a carbon-based fuel. Initially, solar energy is captured by the light harvesting pigment-protein complexes. In purple bacteria these antenna complexes are constructed on a rather simple modular basis. Light absorbed by these antenna complexes is funnelled downhill to reaction centres, where light drives a trans-membrane redox reaction. The light harvesting proteins not only provide the scaffolding that correctly positions the bacteriochlorophyll a and carotenoid pigments for optimal energy transfer but also creates an environment that can modulate the wavelength at which different bacteriochlorophyll molecules absorb light thereby creating the energy funnel. How these proteins can modulate the absorption spectra of the bacteriochlorophylls will be discussed in this review.

Carotenoid Analysis from Commercial Banana Cultivars (Musa spp.) in Malang, East Java, Indonesia Jodiawan Jodiawan; Devi Natalia Chrisdiyanti; Nur Vi’atin; Monika Nur Utami Prihastyanti; Rosita Dwi Chandra; Heriyanto Heriyanto; Chandra Ayu Siswanti; Lia Hapsari; Tatas Hardo Panintingjati Brotosudarmo
Indonesian Journal of Chemistry Vol 21, No 3 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.60865

Banana, as the world’s most consumed fruit, is a good source of carbohydrate, potassium, fiber, and other essential nutrients such as pro-vitamin A carotenoids. The carotenoid content and composition in bananas commonly sold in fruit shops in Malang have become interesting studies. Five types of banana, i.e. Berlin, Mas, Cavendish, Candi and Raja were characterized using high-performance liquid chromatography and UV-Vis spectrophotometer. The carotenoid concentrations ranged between 347–3,660 µg/100 g dry weight (dw), with the level of vitamin A activity at the range between 6-139 µg RAE/100 g dw. The Mas banana had a higher carotenoid concentration compared to the Berlin, Candi, Raja, and Cavendish varieties.

Optimization of Adsorption and Desorption Time in the Extraction of Volatile Compounds in Brewed Java Arabica Coffee Using the HS-SPME/GC-MS Technique Lidwina Angelica Soetantijo; Hendrik O. Lintang; Heriyanto Heriyanto; Mitha Ayu Pratama Handojo; Tatas Hardo Panintingjati Brotosudarmo
Jurnal Kimia Sains dan Aplikasi Vol 25, No 2 (2022): Volume 25 Issue 2 Year 2022
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

The headspace solid phase microextraction (HS-SPME) technique has been recognized as a reliable technique for characterizing the aroma profile of Arabica coffee beans. The amount and content of the detected volatile compounds depend on the volatile analyte extraction process with HS-SPME, namely the adsorption and desorption processes. However, the optimal extraction time in applying coffee volatile compounds is still limited. This research aimed to obtain the optimum adsorption and desorption time in analyzing volatile compounds in brewed Java Arabica coffee. The adsorption time was optimized for 20 to 60 minutes with 5 minutes desorption time. The desorption time was optimized from 5 to 45 minutes with a 20 minutes of adsorption time. There are 14 volatile compounds with a peak area percentage of more than 2% from adsorption and desorption optimization. The optimal adsorption time was 50 minutes, where there were 5 of 7 compounds with the most significant area, such as 2-furfural (29%), 2-acetyl furan (3%), 2-furfuryl acetate (6%), 5-methyl furfural (12%), and 2-furfuryl alcohol (14%). Meanwhile, the most optimal desorption time was 5 minutes which detected 12 compounds, while the other desorption time only detected eight compounds. Furfuryl formate (2%), pyridine (12%), and 2-furfuryl alcohol (14%) had a higher peak area than the other compounds at a desorption time of 5 minutes. The results showed the same number of volatile compounds at each adsorption time. In conclusion, the adsorption time did not affect the number of compounds detected as in the optimization of desorption time. Adsorption and desorption time is crucial in analyzing volatile compounds from coffee using the HS-SPME/GC-MS technique.

Effect Of Coffee Drinking Habit to Blood Pressure and Hemoglobin Levels on Women of Childbearing Age Wartiningsih, Minarni; Brotosudarmo, Tatas Hardo Panintingjati; Gonaldy, Vincent Aurelius; Juliasih, Ni Njoman; Silitonga, Hanna; Tanzilia, May Fanny
Journal of Epidemiology and Public Health Vol. 9 No. 3 (2024)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26911/jepublichealth.2024.09.03.01

Background: Tenggerese people, especially women, have a habit of drinking coffee, and it has become a daily habit. Coffee is often associated with risk factors for diseases in the community such as anemia and hypertension. This study aims to determine the effect of coffee drinking quantity (1-2 cups, 3-4 cups, and ≥ 5 cups of coffee per day) on hemoglobin levels and blood pressure. Subjects and Method: A quantitative study with a cross-sectional study design was done. This study population was women of reproductive age (15 to 49 years) in Ngadipuro Hamlet, Tengger, East Java, Indonesia. The independent variable of this study is the of daily coffee drinking per cup and the dependent variable of this study is the hemoglobin levels in women of childbearing age. The study instrument used was a questionnaire to screen for those who were a daily coffee drinker and a blood hemoglobin rapid test kit was used to measure the level of hemoglobin.The number of samples in this study was 193 respondents which was chosen using the random sampling technique. Analysis of the quantity of coffee drinking on hemoglobin levels by the Annova test while on blood pressure by the Kruskal Wallis test through SPSS software. Results: The result showed that the quantity of coffee per day on hemoglobin levels had a significance p<0.001. While the quantity of coffee per day on blood pressure had a significance p=0.361. Conclusion: It can be concluded that drinking 1-2 cups of coffee, 3-4 cups, and ≥ 5 cups of coffee daily simultaneously affects hemoglobin levels. In this case, the more quantity of coffee per day, the lower the hemoglobin level. However, the quantity of coffee had no significant effect on blood pressure.

Does Drinking Coffee and Tea Affect to the Hemoglobine Level on Women of Reproductive Age at Tengger ? – A Preliminary Research Wartiningsih, Minarni; Brotosudarmo, Tatas Hardo Panintingjati; Fanny Tanzilia, May; Kodrat, David Sukardi; Sugiyatmi, Tri Astuti
Jurnal Aisyah : Jurnal Ilmu Kesehatan Vol 8, No 3: September 2023
Publisher : Universitas Aisyah Pringsewu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30604/jika.v8i3.2356

Drinking coffee and tea is a habit of the people of Tengger, East Java, Indonesia, including the women. The polyphenol content in coffee and tea, such as caffeine and tannin, is thought to be one of the factors that can inhibit the absorption of iron in the body. Polyphenols can bind to iron ions to produce compounds that are not useful and will be excreted from the body. So the excessive consumption of polyphenols is thought to cause a decrease in hemoglobin levels wichh indicates anemia. This research was conducted using the cross sectional method and processed using the SPSS application. The result showed that 94% of respondents consumed coffee, 96% consumed tea and 93% consumed coffee and tea every day. In general, the result showed that women of childbearing age with a high frequency of drinking coffe or tea without a mixture had a tendency to have relatively lower hemoglobin levels, compared to those who consumed coffee or tea with a mixture. Menawhile, data related to the habit of drinking coffee and tea without mixture shows relatively lower hemoglobin levels, compared to drinking coffee and tea with a mixture.

Effect Of Coffee Drinking Habit to Blood Pressure and Hemoglobin Levels on Women of Childbearing Age Wartiningsih, Minarni; Brotosudarmo, Tatas Hardo Panintingjati; Gonaldy, Vincent Aurelius; Juliasih, Ni Njoman; Silitonga, Hanna; Tanzilia, May Fanny
Journal of Epidemiology and Public Health Vol. 9 No. 3 (2024)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26911/jepublichealth.2024.09.03.01

Background: Tenggerese people, especially women, have a habit of drinking coffee, and it has become a daily habit. Coffee is often associated with risk factors for diseases in the community such as anemia and hypertension. This study aims to determine the effect of coffee drinking quantity (1-2 cups, 3-4 cups, and ≥ 5 cups of coffee per day) on hemoglobin levels and blood pressure. Subjects and Method: A quantitative study with a cross-sectional study design was done. This study population was women of reproductive age (15 to 49 years) in Ngadipuro Hamlet, Tengger, East Java, Indonesia. The independent variable of this study is the of daily coffee drinking per cup and the dependent variable of this study is the hemoglobin levels in women of childbearing age. The study instrument used was a questionnaire to screen for those who were a daily coffee drinker and a blood hemoglobin rapid test kit was used to measure the level of hemoglobin.The number of samples in this study was 193 respondents which was chosen using the random sampling technique. Analysis of the quantity of coffee drinking on hemoglobin levels by the Annova test while on blood pressure by the Kruskal Wallis test through SPSS software. Results: The result showed that the quantity of coffee per day on hemoglobin levels had a significance p<0.001. While the quantity of coffee per day on blood pressure had a significance p=0.361. Conclusion: It can be concluded that drinking 1-2 cups of coffee, 3-4 cups, and ≥ 5 cups of coffee daily simultaneously affects hemoglobin levels. In this case, the more quantity of coffee per day, the lower the hemoglobin level. However, the quantity of coffee had no significant effect on blood pressure.

Ragam Metode Ekstraksi Karotenoid dari Sumber Tumbuhan dalam Dekade Terakhir (Telaah Literatur) Maleta, Hana Susanti; Indrawati, Renny; Limantara, Leenawaty; Brotosudarmo, Tatas Hardo Panintingjati
Jurnal Rekayasa Kimia & Lingkungan Vol 13, No 1 (2018): Jurnal Rekayasa Kimia & Lingkungan (June, 2018)
Publisher : Chemical Engineering Department, Syiah Kuala University, Banda Aceh, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23955/rkl.v13i1.10008

Karotenoid adalah pigmen yang memberikan warna kuning, jingga hingga merah. Karotenoid merupakan pigmen pendamping klorofil atau zat hijau daun yang menjalankan fungsi penyerapan energi cahaya untuk fotosintesis. Sumber karotenoid utama adalah tumbuhan, yang selanjutnya dikonsumsi dan dimetabolisme atau terakumulasi dalam tubuh hewan. Terdapat lebih dari 3000 jenis karotenoid, dan beberapa jenis telah diketahui memiliki manfaat bagi kesehatan. Beberapa studi menyebutkan karotenoid berfungsi sebagai antioksidan, antikanker, dan membantu memelihara kesehatan mata. Ekstrak karotenoid telah diaplikasikan dalam berbagai produk pangan dan nutrasetikal. Dengan demikian, perkembangan teknologi metode ekstraksi karotenoid menjadi penting untuk diikuti. Ekstraksi karotenoid dilakukan dengan metode konvensional seperti maserasi ataupun modern seperti cairan superkritis, ultrasound asssisted extraction, enzimatik, pulsed electric field, dan pressurized liquid extraction. Sejumlah studi metode ekstraksi karotenoid dalam dekade terakhir disajikan dan dibahas dalam review berikut.

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