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ANALISIS PROKSIMAT DAN FITOKIMIA BUAH PEDADA (Sonneratia ovata Back.) Maria Dewi Astuti; Mahrita Wulandari; Kholifatu Rosyidah; Radna Nurmasari
Jurnal Sains dan Terapan Kimia Vol 15, No 2 (2021)
Publisher : Program Studi Kimia, Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (449.797 KB) | DOI: 10.20527/jstk.v15i2.10728

Abstract

Penelitian tentang analisis proksimat dan fitokimia buah pedada (Sonneratia ovata Back.) telah dilakukan.  Tumbuhan pedada (S. ovata Back.) telah digunakan sebagai bahan pangan ataupun sebagai bahan obat tradisional oleh masyarakat di Kotabaru, Kalimantan Selatan. Hal ini berkaitan erat dengan zat gizi dan fitokimia dari buah tersebut. Penelitian ini bertujuan untuk mengetahui kadar proksimat dan kandungan fitokimia pada buah S. ovata Back.. Penetapan kadar proksimat meliputi kadar air, abu, protein, lemak, dan karbohidrat. Analisis fitokimia meliputi skrining fitokimia dan penetapan kadar saponin berdasarkan metode gravimetri dan flavonoid berdasarkan metode spektrofotometri. Hasil penelitian menunjukkan buah pedada (S. ovata Back.) memiliki kadar air 64,28%, abu 1,04%, lemak 1,80%, protein 9,33%, dan karbohidrat 2,19%. Skrining fitokimia menunjukkan buah pedada mengandung flavonoid, saponin, karotenoid, dan steroid. Kadar saponin total sebesar  0,99% dan kadar flavonoid total sebesar 4,6154 mgQE/g.
UJI ATRAKTAN PADA EKSTRAK PURUN TIKUS (Eleocharis dulcis) SEBAGAI PERANGKAP HAMA PENGGEREK BATANG PADI PUTIH Kholifatu Rosyidah; Zacky Insan; Dahlena Ariyani
Jurnal Hutan Tropis Vol 9, No 2 (2021): JURNAL HUTAN TROPIS VOLUME 9 NOMER 2 EDISI JULI 2021
Publisher : Lambung Mangkurat University-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jht.v9i2.11289

Abstract

Purun tikus (Eleocharis dulcis) are wild plants found on swamp land which are quite abundant in South Kalimantan. This research was conducted to find out the most active extract from Chinese water chestnut as an attractant of white rice stem borer (Scirpophaga innotata). Purun tikus were made into extracts using the stepwise extraction method using n-hexane, ethyl acetate, and methanol, with yields of 0.74%, 0.81%, and 7.58%, respectively. The results of the attractant test on purun tikus extract showed that the extract of n-hexane purun tikus was the most active extract as an attractant in white rice stem borer (Scirpophaga innotata). The results of the analysis using GC-MS showed that the extract of n-hexane purun tikus contained several compounds, namely 2-methyltetrahydrofuran, isopulegol, citronellal, citronellyl acetate, βcitronellol, Z-citral, 1-bromo-3,7-dimethyl-2,6-octadiene, citral, linalyl acetate, trans-caryophyllen, trans-phytol, verticellol.
ISOLASI DAN UJI SITOTOKSIK SENYAWA LIMONEN DARI KULIT BATANG KASTURI (Mangifera casturi) Kholifatu Rosyidah
Bioscientiae Vol 8, No 2 (2011): Bioscientiae Volume 8 No 2
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.888 KB) | DOI: 10.20527/b.v8i2.191

Abstract

The air-stem barks of M.casturi were extracted with Methylene Chloride (MTC). The MTC extract’s had treatment with Brine Shrimp Lethality Test (BSLT) than analyzed with probit and resulted LC50= 47,31 ppm. MTC extract’s was fractionated by Vacuum Liquid Chromatography and recrystalization obtained pure compounds. The compounds was analyzed with GC-MS (Gas chromatography-Mass Spectroscopy) and cytotoxic test with cancer cell Leukemia P388. This compound is limonene and have IC50=70 ppm.
Uji Sitotoksik Ekstrak Alkaloid Daun Belimbing Wuluh (Averrhoa bilimbi L.) terhadap Larva Udang (Artemia salina) Mariani Mariani; Kholifatu Rosyidah; Kamilia Mustikasari
Jurnal Natural Scientiae Vol 1, No 1 (2021): November 2021
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jns.v1i1.4421

Abstract

Research on cytotoxic test of alkaloids extract of belimbing wuluh leaves has been conducted. The objective of this research are to determine the yield, toxicity, TLC chromatogram pattern, and compounds in alkaloids extract of belimbing wuluh leaves with GC-MS. Alkaloid extraction process uses acid base method, the phytochemical tests was carried out with reagents Dragendorff, Wagner and Meyer. Toxicity test of alkaloids extract of belimbing wuluh leaves with BSLT method on A. salina larvae to determine LC50 value. Alkaloids extract of belimbing wuluh leaves were analyzed by TLC and GC-MS. The yield of alkaloids extract of belimbing wuluh leaves obtained was 10, 13 %. The results of phytochemical tests were carried out which showed positive containing alkaloids. LC50 value of the alkaloids extract of belimbing wuluh leaves indicated that is very toxic. The LC50value obtained is 9.170 ppm. Analysis using TLC with eluent of n-hexane: ethyl acetate (8:2) showed 5 stains. GC-MS analysis showed are alkaloid compound with an odd molecular weight.
Personal Hygiene Practices To Reduce The Risk Of Soil-Transmitted Helminth Infection In Farmers Rosyidah, Kholifatur; Maulidia, Aulia; Indrasasi, Adzraa Sadira Ranu; Kurniyawan, Enggal Hadi; Afandi, Alfid Tri; Nur, Kholid Rosyidi Muhammad; Kurniawan, Dicky Endrian
International Journal of Midwifery and Health Sciences Vol. 2 No. 2 (2024): IJMHS Vol 2 No 2 (2024)
Publisher : Rajaki of Tulip Medika Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61777/ijmhs.v2i2.75

Abstract

Backgrounds: Soil-transmitted Helminths infections have become a frequent phenomenon among farmers due to their lack of hygiene, making it easy for nematode worms to enter the body. Soil-transmitted helminths can affect nutritional status, resulting in anemia, loss of appetite, and damage to the intestines. To overcome this phenomenon, adequate personal hygiene in farmers is needed. Purpose: The purpose of this study is to analyze how personal hygiene can affect the incidence of Soil-Transmitted Helminths infection. Methods: The method used is a literature review using 10 kinds of literature from 3 sources, namely Google Scholar, PubMed, and Science Direct with a publication range from 2020-2024. Result: The results showed that some articles showed an association between personal hygiene and the incidence of soil-transmitted helminths. Good personal hygiene can prevent the entry of nematode worms into the digestive tract of the body. Besides that, some articles also showed no association between personal hygiene and soil-transmitted helminth infection. Conclusion: Adequate personal hygiene such as always using gloves and complete personal protective equipment while working, always washing hands before eating, and maintaining environmental hygiene is needed by farmers to avoid the incidence of soil-transmitted helminth infection.
Pengaruh Waktu Reaksi Terhadap Viskositas Dan Densitas Tetraetil Ortosilikat Dari Silika Abu Sekam Padi Mujiyanti, Dwi Rasy; Nisa, Hayatun; Rosyidah, Kholifatu; Ariyani, Dahlena; Abdullah, Abdullah
Indonesian Journal of Chemical Research Vol 8 No 1 (2020): Edisi Bulan Mei (Edition for May)
Publisher : Jurusan Kimia, Fakultas Sains dan Teknologi, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2020.8-dwi

Abstract

Tetraethyl Ortosylicate (TEOS) is a material is widely used in industrial fields. One source of silica (SiO2) is rice husk ash. In this study was determined the effect of reaction time on viscosity and density in making TEOS from silica rice husk. Silica resulting from the purification of rice husk ash is used in the TEOS manufacturing process by examining the variation of reaction time. One mole of ethanol and 0.25 mole of silica powder were added into 250 ml of round bottom flask followed by the addition of 1 gram of CuO/Al2O3 catalyst then the mixture was refluxed for 30, 35, 40, 45 and 50 hours with sufficient stirring and a temperature of 90 °C. The FTIR characterization results show that there are three main functional groups, namely the -OH, Si-O and C-O groups in the five TEOS synthesis results. Wavenumbers of the –OH functional groups obtained ranged from 3349 cm-1 - 3315 cm-1; Si-O functional groups range from 813 cm-1 - 606 cm-1 and C-O functional groups range from 1105 cm-1 - 1040 cm-1. Reaction time has no significant effect on viscosity. Reaction time has no significant effect on the density and density of the resulting TEOS.
Skrining Fitokimia dan Aktivitas Antibakteri Ekstrak Metanol Daun Kalangkala (Litsea garciae Vidal) Idris, Muddatstsir; Rosyidah, Kholifatu; Mustikasari, Kamilia; Kasumawati, Fahrina
Jurnal Pharmascience Vol 12, No 1 (2025): Jurnal Pharmascience
Publisher : Program Studi Farmasi FMIPA Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v12i1.21785

Abstract

Kalangkala (Litsea garciae Vidal) dikenal sebagai tumbuhan dengan berbagai bioaktivitas, menjadikannya kandidat yang menjanjikan untuk aplikasi farmasi dan pengobatan tradisional. Penelitian ini bertujuan untuk  mengetahui kandungan fitokimia dan aktivitas antibakteri pada ekstrak metanol daun tumbuhan tersebut. Metode skrining fitokimia dilakukan  untuk menganalisis  kandungan alkaloid, triterpenoid, flavonoid, dan fenolik dalam ekstrak metanol. Aktivitas antibakteri dievaluasi secara in vitro terhadap bakteri Gram positif (Staphylococcus aureus) dan Gram negatif (Salmonella typhimurium). Hasil penelitian menunjukkan bahwa ekstrak metanol mengandung senyawa triterpenoid, fenolik, dan flavonoid. Pengujian antibakteri menunjukkan bahwa S. aureus menunjukkan zona penghambatan sebesar 12,00±0,08 mm, 16,00±0,12 mm, dan 19,00±0,22 mm pada konsentrasi ekstrak masing-masing 25%, 50%, dan 100%. Demikian pula, S. typhimurium menunjukkan zona penghambatan sebesar 18,00±0,23 mm, 20,00±0,11 mm, dan 22,00±0,07 mm pada konsentrasi yang sama. Hasil ini mengindikasikan potensi L. garciae Vidal sebagai agen antibakteri.