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Lipid Profile (Total Cholesterol, LDL, HDL, Triglyceride) of Injection Contraceptive Acceptors in Semarang Surati, Surati; Priyatno, Djoko
Journal of Maternal and Child Health Vol. 6 No. 5 (2021)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

Background: Hormonal injectable contraceptives are the most common choice taken by women. The 3-month injection of contraception is DMPA, one of the most effective contraceptive methods currently used. Using injectable contraceptives, especially DMPA, influences fat metabolism, especially lipoproteins. Changes in fat metabolism cause disturbances in the balance of blood fat fractions (fluctuation of HDL, LDL and total cholesterol levels) due to hormonal influences. Subjects and Method: Descriptive with an observational design study. Seventy-three women accepted injection contraceptive three months with a usage period of more than three years recorded in the Tlogosari Kulon Community Health Center. They were selected based on sample criteria, including inclusion criteria and exclusion criteria, where these criteria determine whether or not the sample can be used. The dependent was lipid profile (cholesterol, trigliceryd, LDL, HDL). The independent variables was contraceptive injections three months. There are seventy-three respondents. Results: The respondents in this study had 3-month injection contraceptive use of were less than the same as six years of use, namely 41 people, while for use more than the same as seven years there were 32 people and show that most of the research respondents had a lipid profile that was still within normal limits. For the cholesterol levels, 23% is normal, and 76% is abnormal, Trigliceryd level 66% is normal, and 34 % is abnormal. LDL levels 100% is normal, and for the HDL levels, 63% is normal, and 37% is abnormal. Conclusion: Most of the lipid profiles of respondents were normal. However, on cholesterol levels, respondents had more abnormal levels than normal, but for other lipid profiles (Trigliceryd, LDL, HDL), most respondents had normal levels.

Hubungan Kadar Vitamin D3 Dengan Derajat Kepositifan BTA Pada Penderita Tuberkulosis Paru Kuncara, Rachmad Bayu; Istiqomah, Anisa; Hartono, Rodhi; Priyatno, Djoko
Jurnal Analis Kesehatan Vol. 12 No. 2 (2023): Jurnal Analis Kesehatan
Publisher : Department of Health Analyst, Politeknik Kesehatan, Kementerian Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jak.v12i2.3925

Tuberkulosis paru adalah penyakit menular yang disebabkan bakteri Mycobacterium tuberculosis (MTB). Pemeriksaan mikroskopis Bakteri Tahan Asam (BTA) merupakan pemeriksaan yang penting untuk membantu diagnosis tuberculosis paru. Penderita tuberculosis paru dapat mengalami peningkatan jumlah BTA apabila mengalami defisiensi vitamin D. Vitamin D dapat menghambat multiplikasi MTB dalam makrofag dengan induksi peptida anti mikroba. Penelitian ini bertujuan untuk mengetahui hubungan kadar vitamin D3 dengan derajat kepositifan bakteri tahan asam pada penderita tuberkulosis paru. Jenis penelitian ini adalah observasional analitik dengan desain cross sectional. Sampel penelitian sebanyak 30 responden diambil dari Puskesmas Bangetayu, Puskesmas Tlogosari Wetan, dan Puskesmas Kedungmundu di Kota Semarang. Sampel tersebut diperiksa BTA secara mikroskopis dan pemeriksaan vitamin D3 dilakukan dengan metode Enzym Linked Immunosorbent Assay (ELISA). Hasil pemeriksaan BTA menunjukan sebanyak 12 (40,0%) penderita dengan hasil scanty, sebanyak 7 (23,3%) penderita positif 1, sebanyak 7 (23,3%) penderita positif 2 dan 4 (13,4%) penderita positif 3. Hasil pemeriksaan vitamin D menunjukan sebanyak 23 (76,7%) penderita dengan status defisiensi, 5 (76,7%) penderita dengan status insufisiensi dan 2 (6,7%) penderita dengan status sufisiensi. Data tersebut dianalisa secara statiskik menggunakan uji Spearman dengan hasil p-Value = 0,023 (p< 0,05) sehingga dapat disimpulkan terdapat hubungan kadar vitamin D3 dengan derajat kepositifan BTA pada penderita tuberculosis paru.

Hubungan Tingkat Pengetahuan tentang Diabetes Melitus, Tingkat Asupan Karbohidrat dan Tingkat Asupan Serat dengan Kadar Glukosa Darah Postprandial pada Penderita Diabetes Melitus Tipe 2 Rawat Jalan RSUP Dr. Kariadi Semarang Rahmawati, Pradina; Mintarsih, Sri Noor; Priyatno, Djoko
JURNAL RISET GIZI Vol 1, No 1 (2013): Mei 2013
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jrg.v1i1.37

Background: the prevalence of type 2 diabetes mellitus in the province of Central Java in 2009 at 0, 19% experienced improvement when compared to the prevalence in 2008 of 0, 16%. incidence of diabetes mellitus is influenced by several factors. knowledge about the disease diabetes mellitus diabetes mellitus and its treatment is important in holding peranana control blood sugar levels. intake of carbohydrates and fiber that fits your needs can help control blood sugar levels. Purpose: This study aims to determine the relationship level of knowledge about diabetes mellitus, the level of carbohydrate intake and level of fiber intake with postprandial blood glucose levels in patients with type 2 diabetes mellitus poly outpatient department of Dr. Kariadi Semarang. Methods: the study design was cross sectional approach. Data rates of diabetes mellitus knowledge obtained by a questionnaire, the data rate of carbohydrate intake and level of fiber intake obtained by food frequency questionnaire semi-quantitative. data analysis using chi square test. Results: the results showed that all samples were aged over 40 years, the number of samples of postprandial blood glucose levels are high as 51.5%, the level of knowledge of diabetes mellitus with less category at 72.7% and a high level of carbohydrate intake 72.7 % and the level of fiber intake by as much as 72.7% less category. Conclusion: there is a relationship between the level of knowledge about diabetes mellitus in post prandial glucose levels, while the level of carbohydrate intake and level of fiber intake did not show any relationship with post-prandial blood glucose levels. need to make a referral to outpatients and provide counseling on a regular basis.

Deskripsi Skrining Tuberkulosis Pada Anak Penderita Tuberkulosis Di Puskesmas Bangetayu Amirul Baldi, Wildan; Priyatno, Djoko; Surati, Surati
Jurnal Media Analis Kesehatan Vol 15 No 2 (2024): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Potekkes Kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/jmak.v15i2.811

Tuberkulosis adalah penyakit menular yang menjadi salah satu penyebab utama kematian di dunia. Pemeriksaan metode Immunochromatography Tuberculosis (ICT-TB) merupakan salah satu alternatif deteksi dini adanya kasus baru terhadap penderita tuberkulosis anak. Tujuan penelitian ini yaitu untuk mendeskripsikan skrining tuberkulosis pada anak penderita tuberkulosis dengan metode Immunochromatography Tuberculosis. Metode penelitian ini merupakan penelitian observasional deskriptif. Hasil pemeriksaan tuberkulosis paru metode Immunochromatography Tuberculosis dengan mengambil sampel darah kapiler anak menggunakan autoclick didapatkan bahwa seluruh sampel penelitian dinyatakan negatif penyakit tuberkulosis paru, yaitu sebanyak 24 sampel (100%). Karakteristik penelitian berdasarkan jenis kelamin didapatkan laki-laki 11 anak (46%) dan perempuan 13 anak (54%). Berdasarkan usia balita 4 anak (16%), anak-anak 10 anak (42%), dan remaja 10 anak (42%). Berdasarkan status gizi didapatkan status gizi kurang 5 anak (62%) dan status gizi normal 9 anak (38%). Kesimpulan dari hasil penelitian ini yaitu pemeriksaan tuberkulosis paru menunjukkan bahwa seluruh sampel penelitian dinyatakan negatif penyakit tuberkulosis paru yang dilakukan menggunakan metode Immunochromatography Tuberculosis.

Hubungan Tingkat Pengetahuan tentang Diabetes Melitus, Tingkat Asupan Karbohidrat dan Tingkat Asupan Serat dengan Kadar Glukosa Darah Postprandial pada Penderita Diabetes Melitus Tipe 2 Rawat Jalan RSUP Dr. Kariadi Semarang Rahmawati, Pradina; Mintarsih, Sri Noor; Priyatno, Djoko
JURNAL RISET GIZI Vol 1, No 1 (2013): Mei 2013
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jrg.v1i1.37

Background: the prevalence of type 2 diabetes mellitus in the province of Central Java in 2009 at 0, 19% experienced improvement when compared to the prevalence in 2008 of 0, 16%. incidence of diabetes mellitus is influenced by several factors. knowledge about the disease diabetes mellitus diabetes mellitus and its treatment is important in holding peranana control blood sugar levels. intake of carbohydrates and fiber that fits your needs can help control blood sugar levels. Purpose: This study aims to determine the relationship level of knowledge about diabetes mellitus, the level of carbohydrate intake and level of fiber intake with postprandial blood glucose levels in patients with type 2 diabetes mellitus poly outpatient department of Dr. Kariadi Semarang. Methods: the study design was cross sectional approach. Data rates of diabetes mellitus knowledge obtained by a questionnaire, the data rate of carbohydrate intake and level of fiber intake obtained by food frequency questionnaire semi-quantitative. data analysis using chi square test. Results: the results showed that all samples were aged over 40 years, the number of samples of postprandial blood glucose levels are high as 51.5%, the level of knowledge of diabetes mellitus with less category at 72.7% and a high level of carbohydrate intake 72.7 % and the level of fiber intake by as much as 72.7% less category. Conclusion: there is a relationship between the level of knowledge about diabetes mellitus in post prandial glucose levels, while the level of carbohydrate intake and level of fiber intake did not show any relationship with post-prandial blood glucose levels. need to make a referral to outpatients and provide counseling on a regular basis.

Kepatuhan Pemeriksaan PPIA (Pencegahan Penularan HIV dari Ibu Ke Anak) Ibu Hamil dengan Risiko HIV (Human Immunodeficiency Virus) Wibowo, Anang; Priyatno, Djoko
Jaringan Laboratorium Medis Vol. 1 No. 1 (2019): May 2019
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v1i1.5164

Human Immunodeficiency Virus (HIV) is an infectious disease with a death rate and the number of disease incidence is high and requires the diagnosis and the therapy long enough. The success of the program depends on the PPIA socialization of HIV and Syphilis in pregnant women, as well as compliance checked her pregnancy during pregnancy for early detection of HIV and Syphilis that is useful for the treatment of early and proper while the results the examination showed reactive. The purpose of this research to know description of compliance inspection of PPIA (prevention of HIV transmission from mother to child) of pregnant women with HIV risk in public health II in Baturraden. The method is observational approach time crosssectional tests. Population in the study are all pregnant women and pregnant women throughout the research sample of trimester I, timester II, and trimester III who programme PPIA in public health II in Baturraden of this year in 2018 as many as 100 people. The research results obtained compliance examination of the PPIA pregnant women HIV risk i.e. age 35-44 in 76%; secondary education diploma 100%; and employment status of civil servant or the student as much as 100%. While 55% of disobedience at 15-24 years old; elementary school level 73%; and a housewife 59%. The higher the education, age, and occupation of a person then the PPIA program to carry out compliance higher.

Prevalensi Hasil Pemeriksaan HBsAg dan Anti HBs pada PSK (Pekerja Seks Komersial) Priyatno, Djoko; Qomariyah, Nurul
Jaringan Laboratorium Medis Vol. 1 No. 2 (2019): November 2019
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v1i2.5453

Commercial sex workers are a high risk group of STD infection (HIV-AIDS, syphilis, gonorrhea and Hepatitis-B). Transmission of HBV 100x faster than the HIV. Diagnosis HBsAg and Anti-HBs is a marker of HBV infection can be detected at least 2 weeks before clinical symptoms appear. The incidence of STIs Semarang (in 2012) is the highest in the resocialization Argorejo as much as 1,623 cases. Monitoring of Hepatitis B in resocialization has not become a routine examination. The formulation of the research problem is "How prevalence of HBsAg and Anti-HBs on resocialization PSK Argorejo Semarang?". This study is observational analytic cross-sectional design. Specimens research is venous blood PSK Argorejo resocialization who check monitoring of HIV-AIDS in August-September 2015. Â Sampling a total of sampling for examination imunoserologis HBsAg and Anti-HBs in the strip method. Analysis of the relationship between host factors with HBsAg and anti-HBs using Spearman's test. Negative values on the results of HBsAg while the percentage of anti-HBs PSK resocialization some 29%. There is no relationship between age, education and knowledge with Anti-HBs. There is a relationship between sexual behavior with Anti-HBs. Upregulation required screening and immunization of Hepatitis-B infection in the community resocialization Argorejo Semarang.

Studi Literatur Gambaran C-Reactive Protein Pada Penderita Hipertensi Fadilah, Umi; Priyatno, Djoko
Jaringan Laboratorium Medis Vol. 2 No. 2 (2020): November 2020
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v2i2.7696

Hypertension is called the silent killer, often known after complications occur. Based on the results of Riskesdas in 2018, the prevalence of hypertension in Indonesia at age â‰¥18 years increased to 34.1% in 2018. Endothelial injury caused by hypertension triggers inflammation or chronic inflammation of the arterial wall. Evidence of an inflammatory process is the discovery of C-Reactive Protein (CRP) which is a marker of inflammation from non-bacterial infections.The purpose of this study to knowing the description of C-Reactive Protein in patients with hypertension through research by Fadhliani (2016), Dolly (2018), Harahap (2019). This paper is a summary of Â research result, with the criteria for systematic study research through a systematic review approach. In patients with hypertension according to research Fadhliani (2016) obtained as much as 23% C-Reactive Protein positive and 77% C-Reactive Protein negative. In the Harahap study (2019) of 30 people with hypertension, 12 people (40%) had positive C-Reactive Protein and 18 people (60%) had negative C-Reactive Protein. Whereas in the study of Dolly (2018) the average level of hs-CRP in hypertensive patients is 4, 85 mg / L. According to Dolly's research (2018) in patients with hypertension for > 1 year have higher levels of hs-CRP compared to patients with hypertension for a period of â‰¤1 years. Examination of C-Reactive Protein (CRP) in patients with hypertension in the Harahap study (2019) showed positive CRP results dominated by subjects with age â‰¥ 50 years as many as 8 people (27%).

Histologi Jaringan Hepar Mencit (Mus Musculus) yang Difiksasi dengan Larutan Carnoy dengan Variasi Waktu 4 Jam, 8 Jam dan 12 Jam Afrida, Arin Dwi; Priyatno, Djoko
Jaringan Laboratorium Medis Vol. 3 No. 1 (2021): May 2021
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v3i1.7920

Histopathological examination is a routine examination process for each tissue in the anatomic pathology laboratory. The stages in making histology preparations are fixation stages. The type of solution that can be used as a fixative solution other than 10% NBF is Carnoy's solution. Carnoy's solution is a fixative solution with a relatively fast fixation process of about 1-4 hours. The advantages of Carnoy's solution can lyse erythrocytes and dissolve lipids, have the ability to maintain cell nuclei, and retain glycogen. The purpose of this study was to determine the histology of the liver tissue preparations of mice (Mus Musculus) which were fixed using Carnoy's solution with time variations of 4 hours, 8 hours, and 12 hours. This is an observational research with descriptive research design criteria. The results of the histology of the liver tissue preparations of mice (Mus Musculus) which were fixed using the 10% NBF solution group obtained 100% good preparations. In the Carnoy solution group with a time of 4 hours, it gave 2.2% results of poor preparations and 97.78% of good preparations. While the treatment group with Carnoy's solution with a time of 8 hours obtained as many as 11.11% of preparations that were not good and 88.89% of good preparations. and in the Carnoy solution group with a time of 12 hours, there were 44.44% of the poor preparations and 55.56% of the good preparations.

Minyak Zaitun Sebagai Pengganti Xylene pada Prosesing Jaringan Histologis untuk Pewarnaan Kulit dan Hepar Mencit dengan Hematoxylin Eosin: Sebuah Studi Perbandingan Sofyanita, Eko Naning; Iswara, Arya; Priyatno, Djoko
Jaringan Laboratorium Medis Vol. 4 No. 2 (2022): November 2022
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v4i2.8688

Clearing results in histological processing can be affected due to tissue density and viscosity of the clearing agent. XyleneÂ is the most commonly used aromatic solvent for clearing agents and deparaffinizing agents in histopathology laboratories, but xyleneÂ is one of the most dangerous chemicals found in histology laboratories.Â Based on the dangers posed by xylene, a replacement material is needed. Several xyleneÂ substitutes such as reagents derived from limonene, aliphatic hydrocarbons, vegetable oils and mineral oils have been commercially developed, but the available xyleneÂ substitutes are still less effective, more expensive, and still as dangerous as xyleneÂ itself. Substitutes that are considered safer are from natural oils, one of which is olive oil which has similarities with xylene, namely in hydrocarbon and phenol compounds. This study used two types of tissue, namely skin and liver from mice (Mus musculus) which were cut into two parts; the first part uses xyleneÂ as and the other part uses olive oil as a clearing agent. The assessment of hematoxylin eosin staining results were determined based on the category of assessment of cell structure, such as the cell nucleus, cytoplasm, and color uniformity carried out by three readers and five fields of view for each microscopic slide at 40X magnification of the objective lens. Comparison of observations of the xyleneÂ group in skin and liver tissue 100% got a good score on the cell nucleus, cytoplasm, and color uniformity. (p=1,000). The olive oil group had a slight difference in color uniformity in liver tissue when compared to skin, but not statistically different (p=0.773). The comparison of the overall readings of the xyleneÂ and olive oil groups on skin and liver tissue also showed no statistically different (p=0.262). So it can be concluded that olive oil can be recommended as a substitute for xyleneÂ in the clearing process in histological tissue processing.

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