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All Journal BIO-EDU: Jurnal Pendidikan Biologi Buguh: Jurnal Pengabdian Kepada Masyarakat OKTAL : Jurnal Ilmu Komputer dan Sains BULLET : Jurnal Multidisiplin Ilmu AMMA : Jurnal Pengabdian Masyarakat Analit: Analytical And Environmental Chemistry
Endah Setyaningrum
Universitas Lampung
Humanities Astronomy Chemistry Computer Science & IT Education Electrical & Electronics Engineering Environmental Science Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Social Sciences

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Bioaktivitas Larvasida Ekstrak Formula Daun Jeruk Nipis (Citrus aurantifolia) Dengan Jeruk Purut (Citrus hystrix) Terhadap Nyamuk Aedes aegypti Sofiya Anggi Salbia; Emantis Rosa; Endah Setyaningrum; Hendri Busman
BULLET : Jurnal Multidisiplin Ilmu Vol. 5 No. 5 (2026): BULLET : Jurnal Multidisiplin Ilmu (INPRESS)
Publisher : CV. Multi Kreasi Media

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Abstract

Dengue Hemorrhagic Fever (DHF), transmitted by Aedes aegypti mosquitoes, remains a public health problem, while the use of synthetic insecticides carries the risk of resistance and environmental pollution. This research aimed to identify the secondary metabolites in lime (Citrus aurantifolia) and kaffir lime (Citrus hystrix) leaf extracts, to determine the most effective extract combination for producing the highest mortality of third-instar Aedes aegypti larvae, and to observe the resulting morphological changes. The research employed a Completely Randomized Design (CRD) with four treatments: a control (distilled water), F1 (10 mL of lime leaf extract + 10 mL of kaffir lime leaf extract), F2 (20 mL of lime leaf extract + 20 mL of kaffir lime leaf extract), and F3 (30 mL of lime leaf extract + 30 mL of kaffir lime leaf extract). Larval mortality percentages were analyzed using the non-parametric Kruskal-Wallis test, followed by Dunn’s post hoc test. Phytochemical screening revealed the presence of alkaloids, flavonoids, tannins, and saponins. The results showed that the treatment containing 30 mL of lime leaf extract + 30 mL of kaffir lime leaf extract was the most effective, with a mean mortality of 95.40%. Morphological observations showed that increasing extract concentrations caused structural damage to Aedes aegypti larvae.
Co-Authors Ainun Rizqi Indriani Ammanda Sadiva Ajeng Pawesti Andinie Putri Arifin Bambang Hermanto Budi Santoso Emantis Rosa Endang L Widiastuti Eti Ernawiati Fatimah Alhafizoh Febrian Cahyadi Hendri Busman Indarto Indarto Indriyani Jani Master Jeany Audina Suryaningkunti Kusuma Handayani Lili Chrisnawati Lisa Vegita M. Kanedi Moh. Dwi Kurniawan Hasan Ni Luh Gede Ratna Juliasih Nida Lidya Susanti Nismah Nukmal Nuning Nurcahyani Salman Farisi Sofiya Anggi Salbia Tugiyono Tugiyono Widiastuti, Endang Linirin Yulianty Soeradji Yulianty Yulianty