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All Journal Jambi Medical Journal : Jurnal Kedokteran dan Kesehatan Jurnal Natur Indonesia Indonesian Journal of Forestry Research Natural B JURNAL BIOLOGI INDONESIA Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Jurnal Akuatiklestari Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Journal of Tropical Marine Science Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Jurnal Abdi Masyarakat Indonesia BIOVALENTIA: Biological Research Journal Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Hippocampus: Jurnal Pengabdian Kepada Masyarakat AMMA : Jurnal Pengabdian Masyarakat Indonesian Science Education Journal (ISEJ) Jurnal Perikanan Indonesian Journal of Forestry Research Berita Biologi Analit: Analytical And Environmental Chemistry

Widiastuti, Endang Linirin

Universitas Lampung

Author-ID : 2616202

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Efek Ekstrak Metanol Makroalga Cokelat (Sargassum sp.), Merah (Gracillaria sp.) dan Taurin Terhadap Gambaran Histopatologi Hepar Mencit Jantan (Mus musculus) yang Diinduksi Benzo(?)Piren Hervidea, Radella; Widiastuti, Endang Linirin; Nurcahyani, Endang; Sutyarso, Sutyarso; Susanto, G. Nugroho
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i1.3670

ABSTRACTCancer is a disease marked by the existence of abnormal growth of cell continuously and uncontroll in an organism body. Benzo(?)pyrene is one of the substances that can cause a cancer. The methanolic extract of Sargassum sp. and Gracillaria sp. has anticancer and antioxidant activity. Other compounds with antioxidant activity is taurine. The aim of this research is to examine the effect from methanolic extract of Sargassum sp. and Gracillaria sp .and also taurine on hepar histopatology of male mice (Mus musculus) induced by benzo(a)pyrene. This research used Completely Randomized Design. 30 male mice were divided into 6 groups of treatments, those were: K1 = (K-), K2= induced by benzo(?)pyrene for 10 days, K3= after induced by benzo(?)pyrene, were given the Sargassum sp. orally with a dose 8mg/mice during 15 days, K4=after induced by benzo(a)pyrene, were given the combination of Sargassum sp. (8mg/mice) and taurine(15,6mg/mice) orally during 15 days, K5= after induced by benzo (?)pyrene, were given the Gracillaria sp.orally with a dose 8mg/mice during 15 days, and K6=after induced by benzo(a)pyrene, were given the combination of Gracillaria sp. (8mg/mice) and taurine (15,6mg/mice) orally during 15 days. Data were analyzed using ANOVA (? = 5%). The result shows that the combination of Sargassum sp. and Gracillaria sp. and taurine can increase the number of erythrocyte , decrease the number of leucocyte and protect the damage of hepar histopatology of male mice (Mus musculus) induced by benzo (?)pyrene. Combination of extract Gracillaria sp. and taurine is the most effective to protect the damage hepar histopatology of male mice(Mus musculus) induced by benzo(? )pyrene.Keywords: male mice,Sargassum sp., Gracillaria sp., taurine, benzo(a)pyrene, hepar

Respon Histopatologis Hepar Mencit (Mus musculus) yang Diinduksi Benzo(α)Piren terhadap Pemberian Taurin dan Ekstrak Daun Sirsak (Annona muricata) Agata, Annisa; Widiastuti, Endang Linirin; Susanto, G. Nugroho; ', Sutyarso
Jurnal Natur Indonesia Vol 16, No 2 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Cancer is a disease that is characterized by the existence of damage and cell abnormality in growth and differentiation. Liver cancer is a disorder of hepar tissue derivated from its tumors. Taurine is known as antioxidant but its role as anticancer needs to be explored more as well the role of Annona muricata leaf extract which is believed to have its role as anticancer substance. This research, therefore, aimed to explore the effect of taurine and Annona muricata leaf extract on the hepar histopathology of male mice (Mus musculus) induced by benzo(α)pyren in vivo. This research was carried out by using a complete randomized design, which consisted of 5 treatment groups which was repeated 5 times. Group I was given 0.2 mL corn oil for 15 days, group II was induced by benzo(α)pyren without taurin nor A. Muricata leaf extract for 10 days, group III was given 7.8 mg taurine/BW/day (twice a day) starting from the 15 th days before the induction of benzo(α)pyren, group IV, after induced with benzo(α)pyren, taurine was given with dosage of 7.8 mg/BW/day, group V, after induced with benzo(α)pyren, soursop leaf extract was given with amount of 277.8 mg/BW/day. Data analyzed by Kruskal-Wallis test and one way ANOVA with Fisher test (p>0.05). The results indicated that taurine had ability to recover the liver tissue induced by benzo(α)pyren as (carcinogenic) while, Annona muricata leaf extract had not shown any recover of tissue damage

Pengaruh Pemberian Senyawa Taurin dan Ekstrak Daun Dewa Gynura segetum (Lour) Merr terhadap Eritrosit dan Leukosit Mencit (Mus musculus) yang Diinduksi Benzo[α]Piren Marlinda, Henny; Linirin Widiastuti, Endang; Susanto, G. Nugroho; ', Sutyarso
Jurnal Natur Indonesia Vol 17, No 1 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Blood cancer (leukemia) is a cancer that occurs due to malignancy of blood cells. Treatment of leukemia generally causes damage to normal cells. Therefore, it needs a drug that has the effect of repairing cell damage and the ability to boost immunity of normal such as taurine and Gynura leaves which are expected to have anticancer and antioxidant properties. The purpose of this study was to investigate the effect of taurine and dewa leaf extract on blood tissues induced by benzo [α] pyrene in vivo, by looking at changes in body weight, the number of red blood cells (erythrocytes), the total number and differentiation of white blood cells (leukocytes) in mice (Mus musculus). Data were analyzed by One Way ANOVA test followed by LSD at 5% significance level. The treatment groups were the group I was given 0.2 mL of corn oil (negative control), group II (given benzo [α] pyrene as a positive control, Group III (given taurine 7.8 mg/BW/day starting from day 1 to 15 before the induction of benzo [α] pyrene until the end of the study), Group IV was given benzo [α] pyrene, then were given taurine 7.8 mg/BW/day were given 2 times a day, as well as the V group was given benzo [α] pyrene, then given a dose of Gynura leaf extract 277.8 mg /BW/day. The results showed taurine and Gynura leaf extract were able to obstruct leukemia by increasing body weight, erythrocyte, leukocyte, and the number of leukocyte differentiation which becomes normal again. In conclusion taurine has better ability for therapoitic than Gynura leaf extact against blood cells induced by benzo[α]piren

Pengaruh Pemberian Taurin terhadap Gambaran Histopatologi Paru Mencit (Mus musculus) yang Diinduksi Karsinogen Benzo(α)Piren secara In Vivo Roselyn, Arini Pradita; Widiastuti, Endang Linirin; Susanto, G. Nugroho; ', Sutyarso
Jurnal Natur Indonesia Vol 17, No 1 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Lung cancer is a disease that causes high mortality. Drugs used to prevent and cure cancer mostly causes intoxicity to the normal tissues due to its less effectiveness. Therefore, it is necessary to find out any agent or substance which works much more effective and safe for cancer treatment. The aim of the study was to elucidate the role of taurine on the lung tissue of mice (Mus musculus) induced by carcinogenic benzo(α)pyrene. The experiment was conducted in a completely randomized design with 5 replications. Six treatment groups were perfomed. Group I was given 0.2 mL of corn oil and given aquadest until the end of the study period, group II was induced by benzo(α)pyrene without administration of taurine, group III before induced with benzo(α)pyrene, was given taurine dosage 7.8 mg/BW/day for two weeks, group IV after induced benzo(α)pyrene, was given taurine with dosage 3.9 mg/BW/day, group V after induced benzo(α)pyrene, was given taurine with dosage7. 8 mg/BW/day, group VI after induced with benzo(α)pyrene, was given taurine with dosage 15.6 mg/BW/day. The results of the Kruskal-Wallis analysis and one way ANOVA with LSD (p>0,05) showed that taurine reduced lung tissue damage 72.73% due to the administration of benzo(α)pyrene of 0.3 mg/BW/day. In addition, the effective dose of taurine reduce lung tissue damage was 15.6 mg/BW/day.

EFEK EKSTRAK METANOL MAKROALGA COKELAT (SARGASSUM SP.), MERAH (GRACILLARIA SP.) DAN TAURIN TERHADAP GAMBARAN HISTOPATOLOGI HEPAR MENCIT JANTAN (MUS MUSCULUS) YANG DIINDUKSI BENZO(?)PIREN Hervidea, Radella; Widiastuti, Endang Linirin; Nurcahyani, Endang; Sutyarso, Sutyarso; Susanto, G. Nugroho
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i1.3670

The Effect of Ethanol Extracts of Jeruju (Holy Mangrove) Leaves (Acanthus ilicifolius L.) and Taurine on Hematopoiesis Profiles of Mice (Mus musculus L.) Induced by Alloxan Ardiansyah, Budi Khoiri; Widiastuti, Endang Linirin; Nurcahyani, Nuning; Sutyarso, Sutyarso
Jurnal Natur Indonesia Vol 18, No 2 (2020): oktober
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.18.2.62-68

The high exposure to free radicals and glucose blood levels in diabetes potentially damage the body cells and decrease body immune status that is shown by changes in the number of blood cells that can cause complications. The purpose of this research was to study the effect of ethanol extracts of jeruju (holy mangrove) leaves (Acanthus ilicifolius L.) and taurine on hematopoiesis profile by measuring the number of erythrocytes, the number of leukocytes and the leukocytes differential. This research used a Completely Randomized Design (CRD) by 5 treatments with 5 repetitions conducted for 14 days. The treatment groups were: K1 negative control, K2 positive control (alloxan induced only), P1 induced by alloxan and given 100% ethanol extract of jeruju leaves at dose of 22.4 mg/bb/day, P2 induced by alloxan and given 50% ethanol extract of jeruju leaves dose of 22.4 mg/bb/day, and P3 induced by alloxan and given taurine dose of 15.6 mg/bb/day. Data were analyzed by ANOVA and followed by LSD test at the 5% level. The results showed that the administration of jeruju and taurin can maintain the number of leukocytes and the number of lymphocyte cells that were close to normal after induced by alloxan. But has no effect in the number of erythrocytes in mice

INNOVATION OF SINBIOTIC FORMULA FOR THE GROWTH OF WHITE SHRIMP LARVAE (Litopenaeus vannamei) Sumardi, Sumardi; Nugroho Ekowati, Christina; Linirin Widiastuti, Endang; Rohmawati Bareta, Ainun; Sarno, Sarno
BIOVALENTIA: Biological Research Journal Vol. 7 No. 2 (2021)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

In the hatchery of white shrimp larvae, there are some problems, such as a decreased water quality and diseases caused by bacteria which cause a decrease in shrimp growth. The solution to reduce these problems is by applying synbiotics. This research aimed to make an innovative synbiotic formula for the absolute length growth and survival rate of white shrimp larvae (Litopenaeus vannamei) as well as the total number of bacteria and the number of Vibrio sp. In this study, there were four treatments, as follows: C-: negative control (without synbiotic application) C+: positive control (commercial synbiotic) P: probiotic 2.5 x 105 cfu/ml and prebiotic 1,5 ppm Q: probiotic 5 x 105 cfu/ml and prebiotic 1 ppm R: probiotic 7,5 x 105 cfu/ml and prebiotic 0,5 ppm S: probiotic 106 cfu/ml and prebiotic 2 ppm, each treatment with four repetitions. This research was set up in a completely randomized design experiment using twenty-four plastic tanks with 50 L total volume filled with 20 L sterile sea water and stocked with 4,000 nauplii in the PT. Citra Larva Cemerlang hatchery, Kalianda, Lampung. Variables observed in this research were survival rate, absolute length growth, total bacterial and Vibrio sp. counts, and water quality in the white shrimp larvae (Litopenaeus vannamei). The results showed that the best survival rate is in treatment S (probiotic 106 cfu/ml and prebiotic 2 ppm) (87.7%), the highest absolute length growth is in treatment P (3.8 mm), the highest total bacteria was in treatment S (11.1 log cfu/ml), and the best total Vibrio sp. is in treatment S (3.5 log cfu/ml). Water quality of the six treatments shows results that are still in normal conditions following Indonesian National Standard SNI 7311: 2009.

PROBIOTIC CANDIDATE PROTEOLYTIC Bacillus sp. COLLECTED FROM MANGROVE OF MARGASARI, LAMPUNG Sumardi Sumardi; Komang Rima; Salman Farisi; Endang Linirin Widiastuti
Indonesian Journal of Forestry Research Vol 8, No 1 (2021): Indonesian Journal of Forestry Research
Publisher : Secretariat of Agency for Standardization of Environment and Forestry Instruments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/ijfr.2021.8.1.73-82

Intensive shrimp culture has encountered many problems, such as declining water quality through disease caused by pathogenic microbes, which affected mortality. This study aimed to determine any potential probiotic from Bacillus sp. collected from mangrove in East Lampung, which could be used to improve the cultured shrimps' proteolytic and probiotic activity. This is a descriptive research with sampling and data collection of bacteria from many samples of mangrove. Result shows 128 isolates Bacillus from which then it has arrived at five potential probiotic Bacillus sp. The study five Bacillus sp. has been isolated with potential properties for probiotic (KPP212, IP121, UJ131, UJ132, SB141). Each isolate has characteristics with proteolytic property, growth in a wide range of pH 4–10 and osmotic stress (0–6% NaCl), non-pathogenic, ability for glucose fermentation, non-motile, and has negative catalase activity. The five potential Bacillus sp. can be used as probiotics for shrimp farming.

Coral Reef Fish and Plankton Diversity toward Coral Reef Coverage in Panjang Island of Anak Krakatoa Mountain Gita Puspitasari; Endang Linirin Widiastuti; Henni Wijayanti; Tugiyono Tugiyono
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 6 No. 1 (2019)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v6i1.31

The coral reef is part of the marine ecosystem that important because of its role as a source of life, especially for marine variety creatures. This study aims to determine the relationship of the diversity of reef fish and plankton to coral reef cover on the Panjang Island of Anak Krakatoa Mount, Lampung. Line Intercept Transect used for retrieval of coral reef data and the Underwater Visual Census method for collecting coral reef fish data. Diversity and dominance index are utilized to analyze correlations between them. A good coral reef is obtained at point 2 (5 meters in depth) while at other points the condition of the coral reef is classified as moderate. The highest number of fish is found at point 1 (5 meters in depth) with 56 individuals. The highest plankton diversity is 3.04 Index whereas located at point 2 (0 meters in depth). They have a positive correlation (0.955-coral reef coverage with fishes, 0.916-diversity of reef fish and coral reefs, 0.833-diversity of reef fish with plankton) upon their relationship.

PENINGKATAN PERTUMBUHAN Daphnia sp. MENGGUNAKAN MEDIA KOTORAN AYAM YANG DICAMPUR DEDAK PADI DENGAN KONSENTRASI BERBEDA Putri Dara Yunda; Sri Murwani; Endang Linirin Widiastuti
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 3 No. 1 (2016)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v3i1.70

Penelitian ini bertujuan untuk mengetahui peningkatan populasi dan laju pertumbuhan populasi spesifik Daphnia sp. pada media kotoran ayam yang dicampur dedak padi dengan konsentrasi berbeda. Penelitian ini dilaksanakan di Laboratorium Biologi Molekuler II Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Lampung pada Bulan Desember 2014. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) terdiri dari 5 perlakuan yaitu P1 (kotoran ayam 100%), P2 (dedak padi 100%), P3 (kotoran ayam 75% + dedak padi 25%), P4 (kotoran ayam 50% + dedak padi 50%), dan P5 (kotoran ayam 25% + dedak padi 75%) dengan pengulangan sebanyak 4 kali pada masing-masing perlakuan. Parameter yang diamati adalah kepadatan populasi, laju pertumbuhan populasi spesifik, dan kualitas air. Data dianalisis dengan analisis sidik ragam (ANOVA) dan dilanjutkan dengan uji Beda Nyata Terkecil (BNT) pada taraf 5%. Hasil penelitian menunjukkan bahwa kepadatan tertinggi populasi Daphnia sp. adalah pada media campuran kotoran ayam 75% + dedak padi 25% yaitu dengan jumlah 970 ind/L, sedangkan kepadatan populasi terendah yaitu pada media menggunakan dedak padi 100% sebesar 730 ind/L. Media kultur terbaik adalah campuran kotoran ayam75% + dedak padi 25% dengan laju pertumbuhan populasi spesifik tertinggi Daphnia sp. yaitu sebesar 43,11%/hari.

Co-Authors ', Sutyarso Abdullah Aman Damai Agata, Annisa Agus Budi Utomo Agus Setyawan Alhafizoh, Fatimah Ardiansyah, Budi Khoiri Arifiyanto, Achmad Arlanda, Revilarita Azizah, Enur Berliana, Melisa Intan Elfa Verda Puspita Endah Setyaningrum Endah Setyaningrum, Endah Endang Nurcahyani Erna Rochana Ernawiati, Eti Fadilah Suci Farisi, Salman Fatimah Alhafizoh Gina Dania Pratami, Gina Dania Gita Puspitasari Hafidz, Muhammad Al Hafidz Hartoyo Hartoyo Hendri Busman Henni Wijayanti Henni Wijayanti Maharani Indra Gumay Febryano Indra Gumay Yudha Juliasih, Ni Luh Gede Ratna Kanedi, M. Kanedi Khairunnisa, Indah Khasana, Devi Ardiyati Khoirunisa Komang Rima Kresna Tri Dewi Leni, Yusyam Lestari, Sepnina Like Lia Anggraini Lili Chrisnawati Marlinda, Henny Mastuti Widianingsih Maya Riantini, Maya Nababan, Yolanda Ni Luh Gede Ratna Juliasih Nida Lidya Susanti Nita Sari Novita Tresiana Novriadi Novriadi Nugroho Ekowati, Christina Nuning Nurcahyani Nurafiah, Eva Nursafitri, Umy Pamungkas, Galuh Rara Primasari Pertiwi Putri Dara Yunda Qadar Hasani Radella Hervidea, Radella Rikha Aryanie Surya Rima, Komang Rizani Rohmawati Bareta, Ainun Romaskila, Ulfanida Roselyn, Arini Pradita RR. Ella Evrita Hestiandari Salman Farisi Saputra, Yosi Dwi Sari, Mutiara Pradita Sarno Sarno Siswahyudianto Sri Murwani Sri Wahyuningsih Sumardi . Sumardi, Sumardi Supono Supono Susanto, G. Nugroho Susanto, G. Nugroho Susanto, Gregorius Nugroho Susanto, Gregorius Nurgroho Sutyarso Sutyarso , Sutyarso Sutyarso Sutyarso Tugiyono Tugiyono Tugiyono Tugiyono Tugiyono Tugiyono Yolanda Nababan Yulianty Yulianty Soeradji Yulianty Yulianty Yuni Astuti

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