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All Journal Buletin Peternakan Jurnal Veteriner Jurnal Ilmiah Aplikasi Isotop Radiasi Jurnal Ilmu Ternak Veteriner JURNAL GALUNG TROPIKA Jurnal Hilirisasi IPTEKS (JHI) Wahana Peternakan Jurnal Biosense Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Ananda Ananda
Universitas Andalas
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary Other

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Journal : Jurnal Bioteknologi

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VITAMIN C SUPPLEMENTATION IN EGG YOLK CITRATE EXTENDER OPTIMIZES BANGKOK ROOSTER SEMEN QUALITY DURING COLD STORAGE Ananda; Faizal, Noval Jumatul; Jaswandi
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11346

Abstract

This study aimed to evaluate the effect of different vitamin C concentrations (0, 0.1, 0.2, and 0.3 g/100 mL) in egg yolk citrate extender and storage time (0, 24, 48, 72, and 96 hours) on the quality of Bangkok rooster semen stored at 5°C. Using a split-plot-in-time design with four replications, semen quality was assessed for motility, viability, abnormality, intact plasma membrane (IPM), and longevity. Results showed a significant interaction (P<0.01) between vitamin C concentration and storage time on motility, and significant effects (P<0.05) on viability, abnormality, and IPM. The 0.2 g vitamin C dose preserved >40% motility and >50% viability for 96 hours and extended sperm longevity up to 11.25±0.95 days. These findings suggest that 0.2 g/100 mL vitamin C is the optimal concentration for preserving rooster semen quality during short-term cold storage.
KINEMATICS OF SPERMATOZOA IN FRESH AND FROZEN SEMEN OF PESISIR CATTLE Ramadhan, Rio; Ananda; Hendri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11410

Abstract

This study aimed to evaluate the quality and kinematics of spermatozoa in fresh and frozen semen of coastal cattle. Semen of coastal cattle was collected using an artificial vagina from 2 males, fresh semen was tested and frozen using tris egg yolk diluent. Fresh and frozen semen of coastal cattle were evaluated using a Computer Assisted Sperm Analyzer (CASA). This study used a t-test analysis by comparing the quality and kinematics of spermatozoa of fresh semen and frozen semen of coastal cattle. The parameters observed were macroscopic, microscopic and CASA quality observations. The results showed the kinematics of fresh and frozen semen motility (83.83%; 71.68%) and the kinematics of progressive motility of fresh and frozen semen (80.81%; 59.56%). Based on the results of the t-test analysis, it was found that t count > t table, the kinematics of fresh semen spermatozoa had a significant effect on the frozen semen of Pesisir cattle on the motility of fresh semen and frozen semen, on the progressive motility of fresh semen and frozen semen. The characteristic values ​​of kinematics in fresh semen and frozen semen of Pesisir cattle were respectively velocity curvilinear (VCL) 178.94 μm/s and 108.52 μm/s; velocity straight line (VSL) 96.61 μm/s and 49.34 μm/s; velocity average path (VAP) 114.99 μm/s and 64.04 μm/s; linearity (LIN) 0.53% and 0.44%; straightness (STR) 0.83% and 0.75%; wobble (WOB) 0.64% and 0.58%; lateral head displacement (ALH) amplitude 5.83 μm and 5.15 μm; beat cross frequency (BCF) 35.28 Hz and 24.65 Hz. The kinematics of fresh and frozen semen spermatozoa significantly affect the value of motility characteristics. The conclusion of the results of this study is that there is a decrease in the quality of fresh semen kinematics and frozen semen kinematics spermatozoa on the value of motility characteristics, namely on the speed of spermatozoa at the velocity curvilinear (VCL), velocity straight line (VSL), and velocity average path (VAP), linearity (LIN), straightness (STR), and wobble (WOB) and on the distance value of spermatozoa amplitude lateral head displacement (ALH) and beat cross frequency (BCF).
Co-Authors - Montesqrit Af&#039;ida Khofsoh Ahmad Dody Setiadi Arif Darmawan Ekayanti Mulyawati Kaiin Elly Roza ENDRI ENDRI Faizal, Noval Jumatul Harif Gusdinal Herbert Sipahutar Hilda Susanty Inggit Kresna Maharsih Jaswandi . Juwita Fitri, Rama Khalil Khalil Kusnadidi Subekti Linda Suhartati M Agus Setiadi Meida Nugrahalia Muhammad Hamzah Solim N Nurhasanah Ni Wayan Kurniani Karja Ramadhan, Rio Rizqan Rizqan Robi Amizar Rusfidra rusfidra Rusli, Ridho Kurniawan Sabrina, S Yulia Yelita Yulia Yellita Zurmiati