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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Agrotrop : Journal on Agriculture Science Jurnal Pengendalian Hayati BIOTROPIA - The Southeast Asian Journal of Tropical Biology AGRIKA Cropsaver : Journal of Plant Protection Plumula : Berkala Ilmiah Agroteknologi Proceeding International Conference on Global Resource Conservation Jurnal Agroekoteknologi BIOEDUSCIENCE Jurnal SOLMA Jurnal Pertanian Agros Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Jurnal Agrotek Tropika Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) JURNAL AGROHITA Jurnal Ilmiah RESPATI Agrotechnology Research Journal Jurnal Pembelajaran dan Biologi Nukleus Agrienvi : Jurnal Ilmu Pertanian Nusantara Science and Technology Proceedings Bioscientist : Jurnal Ilmiah Biologi Berkala Penelitian Hayati AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Eduvest - Journal of Universal Studies Journal of Applied Plant Technology Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Arika Purnawati
Agrotechnology, Faculty Of Agriculture, Universitas Pembangunan Nasional “Veteran” East Java, Surabaya 60294, Indonesia
Aerospace Engineering Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Social Sciences Veterinary Other

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Selection and Formulation of Endophytic Bacteria as Plant Resistance Elicitor against Wilt Disease of Tomato Arika Purnawati; Wiwik Harjani; Herry Nirwanto
Agrotechnology Research Journal Vol 3, No 2 (2019): Agrotechnology Research Journal
Publisher : Perkumpulan Agroteknologi/Agroekoteknologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.264 KB) | DOI: 10.20961/agrotechresj.v3i2.33866

Abstract

Wilt disease of Solanaceae caused by Ralstonia solanacearum reduce the crop quality and negatively affect the crop product. The objective of this research was to discover of endophytic bacteria formulation that effectively decreases bacterial which cause wilt disease on Solanaceae. The research consisted of purification of Ralstonia solanacearum, endophytic bacteria were obtained from the sample and the screening of endophytic bacteria using this following assay: antagonist assay, seedling assay and in planta assay. The results showed that in antagonist assay, the bacterial isolate code PS1, PS2, and PS8 could inhibit growth of R. solanacearum. From the seedling assay, it obtained that all of the isolates increased of percentage of germination, seed coating and powder formulation can decrease disease incidence of bacterial wilt disease.
Natural Ingredients of Beans as Growth Medium and Propagation of Endophytic Bacteria from Lowland Eggplant Arika Purnawati; Safira Rizka Lestari; Iis Purnamawati
Nusantara Science and Technology Proceedings 7st International Seminar of Research Month 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3308

Abstract

Endophytic bacteria are defined as a group of microbes that live in plant tissues that do not cause disease and are mutually related to the host plant, can be isolated from all parts of the plant were roots, stems, leaves, and seeds. Endophytic bacteria have the potential to produce natural products for agriculture, increase plant growth, and support the sustainable development of agriculture. The growth of endophytic bacteria in vitro generally uses a medium that contains nutrients including macronutrients, namely elements (C, H, O, and N), micronutrients, namely non-metallic elements (S and P), metallic elements (Ca, Zn, Na, K, Cu). , Mn, Mg, and Fe), vitamins, water, and energy. To meet these growth requirements, currently the growth and propagation of endophytic bacteria generally still use synthetic media which are expensive and in the field, mainly by farmers, are not easy to obtain. The study aimed to obtain an alternative medium made from beans for the growth and propagation of endophytic bacteria from lowland eggplants. The types of beans were soybeans, green beans, red beans, cowpeas, peanuts, and Nutrient Agar (NA) as control. Inoculation into the medium was done using the spread plate technique. This technique is preceded by a dilution and at dilution of 10-7 was taken 0.1 ml then to be spread on the growing medium. Observations were growing and colony counting was done 24 hours after inoculation. The results were: (1) endophytic bacteria from lowland eggplant can grow well on all mediums with the best growth on NA medium, (2) all media can be used as the propagation of endophytic bacteria from lowland eggplant plants, (3) the assumed of the growth curve is logarithmic with 4 phases were lag phase, exponential phase, stationer phase, death phase.
Formulation of Endophytic Bacteria in Organic Fertilizer as Health Promoter for Corn Plants in Mentaos, Gudo, Jombang Arika Purnawati; Tri Mujoko; Endang Triwahyu Prasetyawati
Nusantara Science and Technology Proceedings 7st International Seminar of Research Month 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3309

Abstract

Corn (Zea mays) has an important role in the food supply in Indonesia, as the second commodity after rice and the second source of carbohydrates after rice which is a derivative of rice and is still considered feasible to meet national needs. The increase in national corn production is strongly supported by several corn production center provinces such as East Java and one of the districts that have the potential to support corn production in East Java is Jombang, but the reality the production experiences several obstacles, one of which is the presence of plant pest organisms (OPT). namely the fungus Peronosclerospora maydis caused by downy mildew. In the control of disease so far in the field, many use fungicides that are harmful to humans and the environment. The solution to reduce the use of fungicides is to utilize endophytic bacteria from eggplant and it has been formulated with organic fertilizer so that it is easier to apply to corn plants, this solution is expected to control disease rates, reduce losses, and improve corn plant health. The method used in community service in Mentaos, Gudo, and Jombang were situation analysis, problem identification, socialization that was attended by 25 farmers, and application of endophytic bacteria from eggplant plants in organic fertilizer formulas. The results obtained are that the people of Mentaos, Gudo, and Jombang were able to apply the endophytic bacterial formula in organic fertilizer with the sprinkle technique. The results show that the community can apply the endophytic bacterial formula, corn plants become healthy, and disease intensity is low.
Beberapa Konsentrasi Ekstrak Umbi Gadung (Dioscorea hispida Dennst.) Sebagai Insektisida Nabati Terhadap Spodoptera litura Fabr. Secara In Vitro Novi Nafisah Najmi; Wiwin Windriyanti; Arika Purnawati
JURNAL PERTANIAN Vol 14, No 1 (2023): Jurnal Ilmiah Respati
Publisher : Universitas Respati Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52643/jir.v14i1.3058

Abstract

Spodoptera litura merupakan hama penting yang dapat menyebabkan kerugian cukup tinggi pada tanaman sawi dan pengendalian yang dilakukan saat ini menggunakan insektisida yang berbahaya untuk manusia dan lingkungan, menyebabkan resistensi hama, resurgensi hama, musnahnya musuh alami, dan residu pestisida pada produk pertanian sehingga perlu cara pengendalian alternatif menggunakan umbi gadung. Umbi mengandung senyawa aktif bersifat racun bagi serangga hama. Penelitian ini dilakukan untuk menguji toksisitas ekstrak umbi gadung terhadap S. litura in vitro, dilaksanakan pada bulan Agustus-Desember 2022 di Laboratorium Kesehatan Tanaman, Fakultas Pertanian, Universitas Pembangunan Nasional “Veteran” Jawa Timur. Rancangan penelitian menggunakan Acak Lengkap (RAL) yang terdiri dari 10 perlakuan, setiap perlakuan diulang 3 kali. Data dianalisis menggunakan analisis ragam dan BNJ 5%. Hasil penelitian adalah : (1) waktu kematian tercepat terjadi pada hari kedua dengan konsentrasi 9% dan 10% pada saat 4 jam setelah aplikasi dan (2) mortalitas tertinggi pada konsentrasi 9% dan 10% sebesar 100%. Nilai terendah pada konsentrasi 10% selama 25,4 jam. Nilai  dan  berturut-turut sebesar 5,40% dan 9,71%.
POTENSI KONSORSIUM Bacillus spp. DAN Pseudomonas fluorescens UNTUK MENGENDALIKAN PENYAKIT LAYU FUSARIUM PADA TANAMAN CABAI RAWIT Nensi Agustina; Arika Purnawati; Endang Triwahyu Prasetyawati
Plumula : Berkala Ilmiah Agroteknologi Vol. 10 No. 1 (2022): Plumula : Berkala Ilmiah Agroteknologi
Publisher : Agrotechnology Study Program, UPN "Veteran" Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Cabai rawit (Capsicum frutescens L.) merupakan salah satu tanaman hortikultura yang bernilai ekonomi tinggi. Produksi cabai rawit seringkali terkendala oleh penyakit layu batang yang diakibatkan cendawan Fusarium sp. Penelitian ini bertujuan untuk mengetahui pengaruh konsorsium bakteri Bacillus spp. dan Pseudomonas fluorescens sebagai agensia hayati pengendali penyakit layu fusarium pada tanaman cabai rawit. Penelitian ini dilaksanakan di screenhouse UPN “Veteran” Jawa Timur. Penelitian dimulai pada bulan Januari 2021 sampai bulan April 2021. Penelitian disusun menggunakan Rancangan Petak Terbagi (RPT) dengan dua faktor yaitu bakteri antagonis dan waktu aplikasi dan diulang sebanyak 4 kali. Faktor pertama yaitu bakteri antagonis dengan 5 taraf perlakuan yaitu Tanpa pemberian bakteri antagonis, Bacillus spp. kode isolat Ba9, Bacillus spp. kode isolat Ba17, P. fluorescens, Bacillus spp. kode isolat Ba9 + P. fluorescens, Bacillus spp. kode isolat Ba17 + P. fluorescens. Faktor kedua yaitu waktu aplikasi dengan 2 taraf perlakuan yaitu sebelum pindah tanam bibit dan setelah pindah tanam bibit. Jika diketahui adanya pengaruh yang nyata dari perlakuan maka dilakukan Uji Jarak Ganda Duncan atau Duncan Multiple Range Test (DMRT) pada taraf 5%. Perlakuan pemberian konsorisum Bacillus spp. kode isolat Ba9 dan P. fluorescens menyebabkan penundaan masa inkubasi Fusarium sp. sebesar 23,42%, meningkatkan tinggi tanaman sebesar 20,55 %, dan mempercepat munculnya calon bunga, sedangkan perlakuan pemberian konsorisum Bacillus spp. kode isolat Ba17 dan P. fluorescens menekan intensitas penyakit layu fusarium 29,03%, meningkatkan jumlah daun sebesar 56,58% dibandingkan dengan pemberian bakteri secara tunggal.
POLA DISTRIBUSI PENYAKIT KRESEK PADA PERTANAMAN PADI DI LAHAN DENGAN TINGKAT KEASAMAN BERBEDA BERBASIS CITRA FOTO UDARA Maria Rodhya Alfa; Herry Nirwanto; Arika Purnawati
Plumula : Berkala Ilmiah Agroteknologi Vol. 10 No. 2 (2022): Plumula : Berkala Ilmiah Agroteknologi
Publisher : Agrotechnology Study Program, UPN "Veteran" Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tanaman padi (Oryza sativa L.) merupakan salah satu tanaman pangan paling penting di dunia setelah jagung dan gandum. Produksi padi mengalami penurunan dikarenakan salah faktor pembatasnya yaitu hawar daun bakteri (HDB) / kresek. Tujuan penelitian ini adalah mengetahui pengaruh derajat keasaman/kebasaan(pH) tanah pada pola sebaran penyakit kresek dan pada lahan pertanaman padi di Sidoarjo melalui pengamatan langsung pada tanaman dan pengamatan melalui foto udara. Penelitian dilakukan dengan mengamati pertanaman padi yang terserang penyakit bakteri pada lahan seluas 800 m2. Pengamatan langsung dilakukan untuk mendapatkan insidensi penyakit dan faktor edafik nilai pH genangan air. Pengamatan melalui foto udara dilakukan untuk memperoleh citra digital lahan padi yang berpenyakit kresek. Data insidensi penyakit diolah secara deskriptif, data nilai pH dibandingkan dengan data pola sebaran, sedangkan citra digital diolah menggunakan metode RGB dengan aplikasi MATLAB R2018a. Pola sebaran penyakit kresek berdasarkan pengamatan langsung di darat dan melalui foto udara adalah mengelompok pada minggu 1 dan 2, lalu acak pada minggu 4 hingga 6. Minggu 3 terdeteksi acak pada pengamatan langsung namun terdeteksi mengelompok pada pengamatan melalui foto udara. Pengamatan melalui foto udara dinilai lebih tinggi validitasnya daripada pengamatan langsung di darat. Pola sebaran penyakit bakteri pada lahan padi tidak dipengaruhi nilai pH.
POLA DISTRIBUSI PENYAKIT KRESEK PADA PERTANAMAN PADI DI LAHAN DENGAN TINGKAT KEASAMAN BERBEDA BERBASIS CITRA FOTO UDARA Alfa, Maria Rodhya; Nirwanto, Herry; Purnawati, Arika
Plumula : Berkala Ilmiah Agroteknologi Vol. 10 No. 2 (2022): Plumula : Berkala Ilmiah Agroteknologi
Publisher : Program Studi Agroteknologi, Fakultas Pertanian, UPN "Veteran" Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33005/plumula.v10i2.81

Abstract

Tanaman padi (Oryza sativa L.) merupakan salah satu tanaman pangan paling penting di dunia setelah jagung dan gandum. Produksi padi mengalami penurunan dikarenakan salah faktor pembatasnya yaitu hawar daun bakteri (HDB) / kresek. Tujuan penelitian ini adalah mengetahui pengaruh derajat keasaman/kebasaan(pH) tanah pada pola sebaran penyakit kresek dan pada lahan pertanaman padi di Sidoarjo melalui pengamatan langsung pada tanaman dan pengamatan melalui foto udara. Penelitian dilakukan dengan mengamati pertanaman padi yang terserang penyakit bakteri pada lahan seluas 800 m2. Pengamatan langsung dilakukan untuk mendapatkan insidensi penyakit dan faktor edafik nilai pH genangan air. Pengamatan melalui foto udara dilakukan untuk memperoleh citra digital lahan padi yang berpenyakit kresek. Data insidensi penyakit diolah secara deskriptif, data nilai pH dibandingkan dengan data pola sebaran, sedangkan citra digital diolah menggunakan metode RGB dengan aplikasi MATLAB R2018a. Pola sebaran penyakit kresek berdasarkan pengamatan langsung di darat dan melalui foto udara adalah mengelompok pada minggu 1 dan 2, lalu acak pada minggu 4 hingga 6. Minggu 3 terdeteksi acak pada pengamatan langsung namun terdeteksi mengelompok pada pengamatan melalui foto udara. Pengamatan melalui foto udara dinilai lebih tinggi validitasnya daripada pengamatan langsung di darat. Pola sebaran penyakit bakteri pada lahan padi tidak dipengaruhi nilai pH.
Potential of The Biological Agent Bacillus spp in Inhibiting Fusarium Wild Disease and its Effects on The Growth and Production of Cayenne Papper (Capsicum frutescens L.) Zakqy, Nauval; Wuryandari, Yenny; Purnawati, Arika
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 10, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2024
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i1.5366

Abstract

Cayenne pepper (Capsicum frutescens L.) is a horticultural crop in Indonesia that often has many obstacles. The main obstacle that is often faced is wilt disease caused by the fungus Fusarium oxysporum. Bacillus spp. bacteria have the potential to control the fungus F. oxysporum. The purpose of this study was to determine the ability of Bacillus spp. bacteria in controlling fusarium wilt disease. This research was conducted from August to November 2023 at the Plant Health Laboratory and screen house of the Faculty of Agriculture, National Development University "Veteran" East Java. The study used a Factorial Complete Randomized Block Design with three replications and nine treatments so that there were 27 experimental units. The observation variables included incubation period, disease intensity, plant height, number of leaves, first flower appearance and fruit wet weight. The research data were analyzed using ANOVA analysis of variance procedure to determine whether there is an effect of each treatment. If it is known that there is a significantly different effect from the treatment, it will be continued with the DMRT test at the 5% level. The results showed that the combination of Bacillus spp bacteria and dosage had an effect on the incubation period and disease intensity. The results showed that the combination of Bacillus spp bacteria and doses had an effect on vegetative phase parameters, namely plant height and number of leaves. However, the combination of Bacillus spp and dosage showed that the generative phase parameters did not significantly affect the first flower appearance and fruit wet weight
Secondary Metabolites of Trichoderma sp. as Antifungal Against Rice Seed-borne Pathogen Fungi Amalia, Anisa Rizki; Purnawati, Arika; Prasetyawati, Endang Triwahyu; Yanti, Vika
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 3: Jurnal Pembelajaran Dan Biologi Nukleus November 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i3.4822

Abstract

The use of quality seeds is necessary to increase rice productivity. The existence of pathogenic fungi infections causes the quality of rice seeds to decrease and impacts the ability to grow plants. Trichoderma sp. is known to produce secondary metabolites that can inhibit the growth of pathogens. This study aimed to examine the ability of the secondary metabolites of Trichoderma sp. at various concentration levels and immersion time to suppress pathogenic fungi infections carrying rice seeds. The research was carried out by giving seed immersion treatment. Then identify the pathogenic fungi the seeds carry for further testing with the blotter test method. The parameters tested were infection rate, infection suppression, and seed viability. The identification results showed types of pathogenic fungi carried by rice seeds included Fusarium sp., Alternaria sp., Aspergillus sp. Penicillium sp., and Rhizopus sp. Research also shows that the secondary metabolites of Trichoderma sp. are effective in improving the quality of rice seeds. The test results showed that the secondary metabolites of Trichoderma sp. can suppress the level of pathogenic fungi infection. There was no interaction between the treatment of metabolite concentrations and immersion time on all test parameters. Seed immersion treatment with secondary metabolites in the 5-20% concentration range has effectively reduced fungi infection rates and increased seed viability. Meanwhile, various levels of soaking time did not affect reducing the level of fungi infection but instead affected the viability of the rice seeds.
Potential of Endophytic Fusarium sp. Against Bacterial Wilt Disease in Tomato Plants Nur Aini Mahfud; Ahmad Adibul Akrom; Arika Purnawati
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi 2023
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2024.4008

Abstract

Ralstonia solanacearum is a pathogen that causes bacterial wilt in tomato plants (Lycopersicum esculentum), can decrease production until 90%. The controls generally use chemical pesticides which are harmful to the environment and humans, so it is necessary alternative controls using endophytic Fusarium sp. The aim of the research was to determine the effect of endophytic Fusarium sp. to bacterial wilt. Endophytic Fusarium sp. was explored on tomato plant in Tlekung, Junrejo-Batu and taken systematically randomly then brought to the laboratory. Isolation of endophytic fungi was done by washing the plants with running water, cutting 1cm then was sterilized. The sample was planted on Potato Dextrose Agar (PDA) and incubated at 37°C for 48 hours, if its growth was purified on new media. Isolation of R. solanacearum was done by washing the stems, cutting 0.5 cm then sterilizing. The piece is put into a test tube, added 10 ml of sterile water until the bacterial mass comes out and dissolves in sterile water. The mass of bacteria in sterile water is shaken using vortex until suspension forms. The suspension was grown on TZC agar and incubated at 280C for 24 hours, if it grow was purified on new media. Application of endophytic Fusarium sp. carried out using immersing technique of the roots of tomato plant. Inoculation of R. solanacearum was done into sterile soil before the plant were planted. The study used completely randomized design (CRD) with control and isolate of endophytic Fusarium sp. as treatments with 3 repeat and conducted in the greenhouse of the Faculty of Agriculture UPN "Veteran" East Java. Observations are incubation period and disease intensity, data were analyzed using BNT 5%. The results show that endophytic Fusarium sp. delayed the period incubation and suppressed the intensity of bacterial wilt disease in tomato plants.
Co-Authors Aditya, Haidar Fari Ahmad Adibul Akrom Akrom, Ahmad Adibul Al Rahmad, Nirmala Putri Alfa, Maria Rodhya Althaf, Marcellino Alvia Cindy Apriliani Amalia, Anisa Rizki Aris Cahyono Bambang HADISUTRISNO Diasi, Ananta Rizki Didik Utomo Pribadi Dwirizki Permata, Aprilla Hendiana Elly Syafriani Endang Triwahyu Prasetyawati Fari A, Haidar Fatimah Nabila Zahra Firmansyah, Moch Ryan Hadi Suhardjono Hasni Wardahni Haykal, Arkan Heriyati, Sukma Herry Nirwanto Iis Purnamawati Ika Noviyanti, Rizky Indah Pemuda Indarwati Indarwati Irsyad Irsyad Ketut Srie Marhaeni Julyasih Khairunnisa, Nabila Zahra Kurniawati Kurniawati Laily, Nurul Laras Setyowati Laras Setyowati Lestari, Safira Rizka Maharani, Salsabilla Diva Mahfud, Nur Aini Marcellino Althaf Maria Rodhya Alfa Maulina Aizah, Shinta Mujoko , Tri Mukaromah, Fathul Naufal, Muhammad Raihan Nensi Agustina Nensi Agustina Nensi Agustina Nijma Nurfadila Noni Rahmadhini Nora Augustien Novi Nafisah Najmi Nur Aini Mahfud Pawana Nur Indah Penta SURYAMINARSIH Prasetyawati, Endang Triwahyu Prasetyowati, Endang Triwahyu Presti Mardiyani Ramadhani Mahendra Kusuma Rizky Ika Noviyanti Saefur Rohman Safira Rizka Lestari Sayekti, Ninik Ari Setyowati, Laras Sri Wiyatiningsih Syamsul Arifin Tri Mudjoko Tri MUJOKO Triwahyu P, Endang Triwidodo ARWIYANTO Widayati, Wilujeng Widyananda, Kanaya Syafina Wilujeng Widayati Windriyanti, Wiwin Wiwik Harjani Yanti, Vika Yenny WURYANDARI Zakaria Wildan Fachrezzy Zakqy, Nauval