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Effectiveness Soaking Duration of Rice Seed with Trichoderma sp. for Controlling Seed-Borne Pathogenic Fungi Mahfud, Nur Aini; Purnawati, Arika; Suryaminarsih, Penta
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i1.6847

Abstract

 Low rice productivity due to the use of low-grade seeds. The quality of the seeds can suffer a decrease caused by the attack of pathogenic fungi carried by the seeds both at the time of planting and storage, which will have an impact on the disruption of plant growth. Biopriming using Trichoderma sp. be an effort to control because it is antagonistic to pathogens. The success of biopriming is also influenced by the duration of seed priming. This study aims to examine the duration of soaking rice seeds with Trichoderma sp. the most effective for lowering seed-borne pathogenic infections. The study was conducted by providing seed soaking treatment using Trichoderma sp. suspension for 6 hours, 12 hours, and 24 hours with PDA medium incubation test method and Growing on Test. The parameters tested are the percentage of infection rate and the percentage of germination. Identification results showed that pathogenic fungi carried by seeds included Rhizopus sp., Fusarium sp., Aspergillus sp., and Mucor sp. The results showed the duration of soaking rice seeds with Trichoderma sp. more effective in decreasing the infection rate is at a 24-hour duration. However, the duration of soaking rice seeds with Trichoderma sp. there was no noticeable difference for the percentage parameter of seed germination.
Application of Fobio Biopesticide in Suppressing the Intensity of Bacterial Leaf Blight Disease in Paddy Plants Using Integrated Pest Management (IPM) in Rice Fields of Puton Village, Diwek District, Jombang Regency, East Java Haykal, Arkan; Wiyatiningsih, Sri; Purnawati, Arika
Eduvest - Journal of Universal Studies Vol. 5 No. 5 (2025): Eduvest - Journal of Universal Studies
Publisher : Green Publisher Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59188/eduvest.v5i5.51233

Abstract

Control of bacterial leaf blight in rice plants is often done by applying chemical pesticides whose future effects will be very bad for the soil and plants that will be planted next. An alternative that can be applied is using the biopesticide Fobio. The aim of this research was to measure the effectiveness of the application of fobio in reducing the intensity of bacterial leaf blight attacks. This research was carried out in June-September 2023 in Diwek District, Jombang Regency. The study used a Randomized Block Design with two factors, namely the Fobio dose consisting of 3 levels, namely P0 (without giving Fobio / 0ml/liter), P5 (dose 5 ml/liter), and P10 (dose 10 ml/liter), the second factor was long The soaking time for seeds using Fobio consists of 3 levels, namely W10 (10 minutes soaking time), W20 (20 minutes soaking time), and W30 (30 minutes soaking time) so there are 9 treatment combinations. Observation parameters include plant height, number of leaves, number of tillers, and intensity of disease attacks. The results of the combination treatment were not significantly different for the number of leaves and number of tillers, for single and combination treatments the plant height and intensity of disease attacks were significantly different. Application of Fobio can increase plant height, number of leaves, and number of tillers on rice plants, and Fobio can suppress bacterial leaf blight attacks that attack rice plants.
POTENTIAL OF ENCAPSULATION Bacillus cereus BTH-22 AGAINTS BACTERIAL WILT DISEASE ON EGGPLANT: - Purnawati, Arika; Triwahyu P, Endang; Fari A, Haidar
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.3736

Abstract

Endophytic bacteria are a group of bacteria that live in plant tissues, potentially as biological agents of plant diseases, especially wilt disease caused by Ralstonia solanacearum and as an inducer of eggplant (Solanum melongena) plant growth. One of these bacteria is Bacillus cereus BTH-22 which was isolated from healthy eggplant stems in Kediri. The purpose and formulation of the problem are to obtain an encapsulation formulation of B. cereus BTH-22 which has the potential as a biological agent against wilt disease caused by R. solanacearum and as an inducer of eggplant (S. melongena) plant growth. The novelty of the research is the encapsulation formulation of B. cereus which is applied to eggplant plants with wilt, because the application generally uses root soaking, watering to the soil surface, mixing with fertilizer, liquid and powder formulations but all are easily washed away by rain. The research method is as follows: (1) Making encapsulation formulation: 75% Na-alginate with 10% aloe vera extract (Na-1) and 75% Na-alginate, 0.875% zeolite and 0.875% sago flour (Na-2), (2) Sterile soil, inoculate R. solanacearum and prepare 1 month old plants, (3) Application of encapsulation formulation was carried out 3 days after inoculation of R. solanacearum (107CFU/mL) using a Completely Randomized Design with 3 replications. Observations were made on days 7, 14, 21, 28, 35, 42, 49 including : disease intensity, plant height, number of leaves, number of flowers. The results of the study showed: viability in the Na-1 and Na-2 treatments was higher than control at the 48th to 96th hours, disease intensity in the Na-1 and Na-2 treatments was lower than control at the 7th to 49th day, plant height in the Na-1 and Na-2 treatments was higher than control at the 14th to 49th day.
POTENSI BAKTERI ENDOFIT ASAL LAHAN BASAH KALIMANTAN SELATAN SEBAGAI AGENSIA HAYATI NEMATODA PURU AKAR (Meloidogyne sp. ) PADA TANAMAN SELEDRI (Apium graveolens L.) SECARA IN VITRO Althaf, Marcellino; Widayati, Wilujeng; Purnawati, Arika
Agros Journal of Agriculture Science Vol 24, No 2 (2022): edisi Juli
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v24i2.1929

Abstract

Seledri (Apium graveolens L.) merupakan tanaman rempah penting yang dibudidayakan di dataran tinggi. Salah satu penyakit yang dapat menurunkan produksi tanaman seledri adalah puru akar yang disebabkan oleh nematode parasit Meloidogyne sp. Pengendalian nematoda sampai dengan saat ini menggunakan pestisida yang toksik, sulit terdegradasi, dan berdampak negatif terhadap lingkungan. Upaya pengendalian biologis saat ini banyak dikembangkan, salah satunya penggunaan mikroorganisme bakteri endofit. Tujuan penelitian: mengetahui potensi bakteri endofit asal lahan basah Kalimantan Selatan sebagai agensia hayati nematode puru akar Meloidogyne sp pada tanaman seledri. Penelitian dilaksanakan dua tahap yaitu : eksplorasi bakteri endofit di lahan basah Kalimantan Selatan Bulan November 2021 dan uji vitro dilaksanakan di Laboratorium Kesehatan Tanaman Fakultas Pertanian Universitas Pembangunan Nasional “Veteran” Jawa Timur Bulan Desember 2021 menggunakan Rancangan Acak Lengkap. Uji dilakukan dengan cara 1 ml suspensi nematoda berisi ± 50 ekor juvenile 2 nematoda hasil ekstraksi dituang dalam 5 ml suspensi bakteri endofit berumur 48 jam yang dipanen menggunakan media NB dan diuji keamanan hayatinya dengan kode isolate BLR 1.2, BLR 1.3, BLR 2.1, BLR 2.2, BLR 2.3, BLR 3.2, AKL 2.1, AKL 2.2, AKL 3.1. Isolate kode BLR 2.2, BLR 1.3 ,BLR 3.2, BLR 2.1 merupakan isolate dengan jumlah mortalitas tertinggi dengan jumlah 50.67%, 45.33%, 49.33% dan 45.33% pada 12 jam setelah perlakuan
POTENSI FORMULASI BIOFUNGISIDA Bacillus sp. (Bth-22) DAN Streptomyces sp. (TMP) TERHADAP PERKECAMBAHAN BENIH JAGUNG Maharani, Salsabilla Diva; Purnawati, Arika; Suryaminarsih, Penta
Agrika Vol. 19 No. 1 (2025): MEI 2025
Publisher : Badan Penerbitan Universitas Widyagama Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31328/ja.v19i1.6754

Abstract

Jagung (Zea mays) adalah salah satu tanaman pangan utama yang memiliki peran penting dalam ketahanan pangan global. Fusarium sp. merupakan patogen tanah yang sering ditemukan pada lahan jagung dan dapat mengganggu perkecambahan dan pertumbuhan tanaman jagung. Penggunaan bakteri Bacillus sp. dan Streptomyces sp. sebagai bahan aktif biofungisida dapat meningkatkan persentase perkecambahan benih dan pertumbuhan tanaman jagung yang terserang patogen Fusarium sp.. Penelitian ini bertujuan untuk mengetahui pengaruh jenis media dan potensi formulasi biofungisida dengan bahan aktif Bacillus sp. dan Streptomyces sp. dalam menunjang perkecambahan dan pertumbuhan tanaman jagung. Penelitian dilakukan dengan Rancangan Acak Lengkap (RAL) dengan dua faktor. Faktor pertama yakni media produksi bakteri: K: Air rebusan kedelai, E: Estrak Kentang Gula (EKG), dan A: Air kelapa. Faktor kedua adalah perbandingan formulasi Bacillus sp. dan Streptomyces sp. yang terdiri atas 4 taraf yaitu BS0 (0:0), BS1 (3:3), BS2 (2:4), dan BS3 (1:5). Hasil penelitian menunjukkan tidak terdapat interaksi antara kedua faktor perlakuan. Perlakuan BS2 merupakan perlakuan terbaik yang memberikan presentase perkecambahan sebesar 82%, hari berkecambah 3.64 HST, jumlah daun pada 35 HST sebanyak 5.7 helai dan 62.8 cm.
Antagonistic Test of Bacillus spp. against Fusarium sp., the Causal Agent of Wilt Disease of Red Chili Plants Heriyati, Sukma; Prasetyawati, Endang Triwahyu; Purnawati, Arika
CROPSAVER Vol 6, No 1 (2023)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v6i1.45386

Abstract

Wilt disease caused by Fusarium oxysporum is one of the diseases affecting chili plants. The utilization of Bacillus spp. can be a solution for plant disease control because it can colonize plants and produce useful microbe compounds to inhibit the development of plant pathogen. This study aimed to determine the ability of Bacillus spp. to suppress the growth of Fusarium sp. isolates from chili plants in vitro. Bacillus sp. isolates Ba-6, Ba-9, Ba-12, Ba-15, Ba-17, and a control (aquadest) were used as treatments, each replicated four times. The observation parameters were the inhibition zone test and microscopic observations of the morphology of Fusarium sp. after the inhibition zone test. The highest inhibition was shown in the treatment of Bacillus sp. isolate Ba-15, which was 20.02%. The observation of Fusarium sp. hyphae after the inhibition zone test showed abnormal growth, which was different for each isolate. Some hyphae were bent, coiled, shrunken, swollen, curled, or lysed.
Efficacy of Endophytic Bacteria as Entomopathogens against Spodoptera frugiperda (Lepidotera: Noctuidae) on Corn (Zea mays L.) Kurniawati, Kurniawati; Purnawati, Arika; Windriyanti, Wiwin
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 14 No. 3 (2025): June 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v14i3.1040-1050

Abstract

The fall armyworm (Spodoptera frugiperda) poses a problem for corn plants (Zea mays L.). The use of endophytic bacteria Bacillus sp. as an entomopathogen is expected to control the S. frugiperda pest. This study aims to determine the effective concentration of the bacteria and the active compounds produced by Bacillus sp. strain Bth 22 in controlling S. frugiperda. The research was conducted from August to October 2024 at the Plant Health Laboratory of the Faculty of Agriculture, UPN "Veteran" East Java, and the Airlangga Research Hub in Surabaya. The study was designed using a completely randomized design (CRD) with bacterial concentration treatments of 0% (control), 10%, 15%, 20%, 25%, 30%, and 35%, repeated five times. Observational parameters included mortality rate, number of pupae and imago formed, as well as the mechanisms and compounds produced by Bacillus bacteria based on HPLC and FT-IR tests. The application of Bacillus sp. Bth-22 affected the mortality parameters, the number of pupae formed, and the number of imago formed. The Bacillus sp. Bth-22 bacteria produced metabolites in the form of hydrocarbon derivatives and amide group compounds, disrupting metabolism and digestion, leading to the mortality of S. frugiperda. Keywords: Entomopathogen, Metabolite compounds, Spodoptera frugiperda, Zea mays 
Metabolit Sekunder Bacillus sp. sebagai Antijamur Jamur Patogen Tular Benih pada Benih Jagung dengan Metode Uji Blotter Setyowati, Laras; Purnawati, Arika; Mujoko , Tri; Mukaromah, Fathul
BIOEDUSCIENCE Vol 8 No 1 (2024): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/12588

Abstract

Background: Seed is a planting material that must have high viability and quality, including being free from pathogens. One of the obstacles in the supply of quality maize seeds is seed-borne fungal pathogen which can affect the quality of maize seeds and cause plant disease. Endophytic bacteria Bacillus sp. has been widely studied to be able to produce secondary metabolites as antifungals. This study aimed to determine the exact concentration of secondary metabolites of endophytic bacteria Bacillus sp. to decrease the infections of seed-borne pathogens fungal on maize seeds using the blotter test method. Methods: This study used a completely randomized design with four concentration levels (10%, 15%, 20%, 25%) and two strain codes of endophytic bacteria Bacillus sp. (Bth-31a and Bth-22). Results: The result of identifying seed-borne fungal pathogen on maize seed included Fusarium sp., Aspergillus flavus, Aspergillus niger, and Rhizopus sp. The Bth-22 treatment with a concentration of 25% could decrease the infection of seed-borne fungal pathogen and had the highest percentage until 50% compared to the control. While the lowest percentage to decrease the infections of seed-borne fungal pathogen occurred in the Bth-31a treatment with a concentration of 10%, which was 32.1% compared to the control. Conclusions: All seed treatments that used secondary metabolites of Bacillus sp. able to decrease the infections of seed-borne fungal pathogen on maize seed compared to the control. In addition, the treatment of secondary metabolites Bacillus sp. is able to increase the germination of maize seeds.
POTENTIAL OF ENCAPSULATION Bacillus cereus BTH-22 AGAINTS BACTERIAL WILT DISEASE ON EGGPLANT: - Purnawati, Arika; Triwahyu P, Endang; Fari A, Haidar
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.3736

Abstract

Endophytic bacteria are a group of bacteria that live in plant tissues, potentially as biological agents of plant diseases, especially wilt disease caused by Ralstonia solanacearum and as an inducer of eggplant (Solanum melongena) plant growth. One of these bacteria is Bacillus cereus BTH-22 which was isolated from healthy eggplant stems in Kediri. The purpose and formulation of the problem are to obtain an encapsulation formulation of B. cereus BTH-22 which has the potential as a biological agent against wilt disease caused by R. solanacearum and as an inducer of eggplant (S. melongena) plant growth. The novelty of the research is the encapsulation formulation of B. cereus which is applied to eggplant plants with wilt, because the application generally uses root soaking, watering to the soil surface, mixing with fertilizer, liquid and powder formulations but all are easily washed away by rain. The research method is as follows: (1) Making encapsulation formulation: 75% Na-alginate with 10% aloe vera extract (Na-1) and 75% Na-alginate, 0.875% zeolite and 0.875% sago flour (Na-2), (2) Sterile soil, inoculate R. solanacearum and prepare 1 month old plants, (3) Application of encapsulation formulation was carried out 3 days after inoculation of R. solanacearum (107CFU/mL) using a Completely Randomized Design with 3 replications. Observations were made on days 7, 14, 21, 28, 35, 42, 49 including : disease intensity, plant height, number of leaves, number of flowers. The results of the study showed: viability in the Na-1 and Na-2 treatments was higher than control at the 48th to 96th hours, disease intensity in the Na-1 and Na-2 treatments was lower than control at the 7th to 49th day, plant height in the Na-1 and Na-2 treatments was higher than control at the 14th to 49th day.
UJI EFEKTIVITAS EKSTRAK BAWANG PUTIH TERHADAP PERTUMBUHAN JAMUR PATOGEN Colletotrichum sp. SECARA IN VITRO Firmansyah, Moch Ryan; Purnawati, Arika; Mujoko, Tri
Agrika Vol. 19 No. 2 (2025): NOVEMBER 2025
Publisher : Badan Penerbitan Universitas Widyagama Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31328/ja.v19i2.7270

Abstract

Peningkatan permintaan cabai di Indonesia diiringi dengan tantangan produksi, salah satunya adalah penyakit antraknosa yang disebabkan oleh Colletotrichum sp. Penyakit ini menurunkan hasil dan kualitas buah, dan umumnya dikendalikan dengan fungisida kimia. Penggunaan fungisida kimia menimbulkan risiko terhadap kesehatan dan lingkungan, sehingga diperlukan alternatif pengendalian hayati. Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak bawang putih sebagai agen hayati terhadap Colletotrichum sp. secara in vitro dan membandingkannya dengan fungisida propineb. Penelitian menggunakan rancangan acak lengkap faktorial (RALF) dengan dua faktor, yaitu jenis perlakuan (ekstrak bawang putih dan fungisida kimia) dan konsentrasi (5%, 10%, dan 15%). Parameter yang diamati meliputi diameter koloni, persentase hambatan, intensitas serangan pada buah, serta karakteristik makro dan mikroskopis jamur. Hasil menunjukkan bahwa ekstrak bawang putih 15% mampu menghambat pertumbuhan jamur sebesar 38.73% dan intensitas serangan sebesar 21.33%, sedangkan fungisida propineb 5% menunjukkan hambatan tertinggi sebesar 78.43% dan intensitas serangan terendah sebesar 2.67%. Hasil ini menunjukkan bahwa ekstrak bawang putih memiliki potensi antijamur. namun efektivitasnya masih lebih rendah dibandingkan propineb.