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All Journal Folia Medica Indonesiana Jurnal Biosains Pascasarjana MPI (Media Pharmaceutica Indonesiana) Journal of Natural Sciences and Mathematics Research THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST PEDAGO BIOLOGI The Indonesian Journal of Infectious Diseases Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Humanism : Jurnal Pengabdian Masyarakat Indonesian Academia Health Sciences Journal KARYA: Jurnal Pengabdian Kepada Masyarakat Empowerment: Jurnal Pengabdian Masyarakat Jurnal Pengabdian Mandiri Prosiding Seminar Nasional Pengabdian Kepada Masyarakat Mukhtabar: Journal of Medical Laboratory Technology Camellia
Yeti Eka Sispita Sari
Universitas Muhammadiyah Surabaya
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Gambaran Cemaran Kapang Kontaminan Pada Buah Pepaya (Carica papaya L) Selama Penyimpanan Yeti Eka sispita Sari; Dita Artanti
PEDAGO BIOLOGI Vol 10 No 2 (2022)
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/pb:jppb.v10i2.17633

Abstract

ABSTRAK Buah pepaya (Carica papaya L) merupakan jenis buah yang menjadi primadona bagi masyarakat karena rasanya yang manis dan kaya akan vitamin serta gizi. Pada kenyataannya kualitas buah pepaya segar maupun saat proses panen sampai pendistribusian tidak langsung bisa dikonsumsi karena adanya cemaran mikroorganisme penghasil mikotoksin yaitu kapang. Hal ini menyebabkan pepaya menjadi cepat busuk dan berbahaya apabila terus dikonsumsi. Tujuan penelitian ini untuk mengetahui gambaran cemaran kapang kontaminan pada buah pepaya (Carica papaya L).. Jenis penelitian yang digunakan adalah deskriptif. Sampel uji sebanyak 30 sampel pepaya yang sudah disimpan pada suhu ruang selama 5 hari dengan luka fisik di buah. Kapang yang tumbuh akan dilakukan pemeriksaan langsung secara mikroskopis menggunakan pewarna Lactophenol Cutton Blue. Berdasarkan hasil penelitian dari 30 sampel buah pepaya (Carica papaya L) ditemukan tiga jenis kapang dengan persentase masing-masing yaitu Aspergillus flavus (60%), Rhizopus oryzae (23,3%) dan Aspergillus niger (16,7%). Hal ini menunjukkan bahwa buah pepaya yang dibiarkan pada suhu ruang dengan adanya luka fisik dapat memicu pertumbuhan kapang. Kata kunci : Buah Pepaya, Aspergillus flavus, Rhizopus oryzae, Aspergillus niger ABSTRACTPapaya fruit (Carica papaya L) is a type of fruit that is a prima donna for the community because of its sweet taste and rich in vitamins and nutrients. In fact, the quality of fresh papaya fruit and during the harvesting process until distribution can not be consumed directly due to the contamination of mycotoxin-producing microorganisms, namely mold. This causes papaya to rot quickly and is dangerous if it continues to be consumed. The purpose of this study was to determine the description of contaminant mold contamination in papaya fruit (Carica papaya L).. The type of research used is descriptive. The test samples were 30 papaya samples that had been stored at room temperature for 5 days with physical injuries in the fruit. The growing mold will be microscopically examined using the Lactophenol Cutton Blue dye. Based on the results of research from 30 papaya fruit samples (Carica papaya L) found three types of mold with a percentage of each, namely Aspergillus flavus (60%), Rhizopus oryzae (23.3%) and Aspergillus niger (16.7%). This suggests that papaya fruits left at room temperature in the presence of physical injuries can trigger mold growth.  Keywords : Papaya Fruit, Aspergillus flavus, Rhizopus oryzae, Aspergillus niger 
Edukasi Dan Sosialisasi Cuci Tangan Pakai Sabun Di KB – TK Aisyiyah Kureksari Pasca Pandemi Covid-19 Anindita Riesti Retno Arimurti; Ellies Tunjung Sari Maulidiyanti; Fitrotin Azizah; Nur Vita Purwaningsih; Dita Artanti; Yeti Eka Sispita Sari
Empowerment: Jurnal Pengabdian Masyarakat Vol. 2 No. 4 (2023): JULI 2023
Publisher : Pusat Riset Manajemen dan Publikasi Ilmiah Serta Pengembangan Sumber Daya Manusia Sinergi Cendikia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55983/empjcs.v2i4.408

Abstract

The COVID-19 pandemic has changed many aspects of our daily lives, including in the school environment. School is an environment where children learn and interact, making them vulnerable to spreading infectious diseases. Washing hands with soap is a simple tion effective in preventing the spread of viruses and maintaining health in schools. Washing hands with soap is practical in killing viruses and bacteria that stick to hands. Soap has properties that can destroy the fat layer of the virus, so the virus cannot survive. By washing hands with soap regularly, children can reduce the risk of contracting COVID-19 and other infectious diseases. This community court activity consists of three stages: preparation, implementation and evaluation. This community service activity was conducted at KB – TK Aisyiyah 1 Kureksari, Sidoarjo. The method used is in the form of posters and extension demonstrations with movement and music methods. After participating in this activity, students can apply hand washing with soap after activities outside the home or before eating.
SOSIALISASI PENTINGNYA SERTIFIKAT HALAL UNTUK PENINGKATAN PELAYANAN UNGGUL SUPLAIER GIZI UNIT PONED PUSKESMAS KEDUNDUNG KOTA MOJOKERTO Muhammad Anas; Luluk Latifah; Andre Ridho Saputro; Yeti Eka Sispita Sari
KARYA: Jurnal Pengabdian Kepada Masyarakat Vol 3 No 3 (2023): KARYA: Jurnal Pengabdian Kepada Masyarakat
Publisher : FKIP Universitas Samawa

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Abstract

Pemerintah melalui Badan Penyelenggara Jaminan Produk Halal (BPJPH) mewajibkan sertifikasi halal baik untuk produk maupun jasa, tidak terkecuali para suplair gizi di sebuah unit pelayanan obstetry neonatal emergensi dasar (PONED) di puskesmas Kedundung. Halal menurut ketentuan syariat Islam adalah sesuatu yang baik, suci atau bersih, dan tidak berbahaya. Sertifikat halal berfungsi sebagai salah satu alat bagi para suplair gizi untuk meningkatkan nilai tambah pelayanan yang dihasilkan, serta memberikan ketenangan bagi pasien, terutama bagi pasien Muslim. Selain itu, para suplair ditantang untuk menerapkan Sistem Jaminan Halal sehingga mutu dan kualitas pelayanan bahkan integritas suatu unit pelayanan menjadi lebih baik di mata pasien. Hal tersebut merupakan kondisi saat ini yang dimanfaatkan oleh Tim Pengabdi. Tim Pengabdi memfasilitasi para suplair gizi unit PONED di puskesmas Kedundung dalam meningkatkan kesadaran akan pentingnya kaidah halal pada produk-produknya melalui sosialisasi dan pendampingan sertifikasi halal.
Identification of Salmonella sp Bacteria in Processed Balado Shrimp Sold in Food Stalls Around Mulyosari Area Mustofa, Nabila Amelia; Sari, Yeti Eka Sispita; Artanti, Dita; Kartikorini, Nastiti
Mukhtabar Journal of Medical Laboratory Technology Vol 2 No 1 (2024): Mukhtabar: Journal of Medical Laboratory Technology (April 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v2i1.545

Abstract

Background & Objective: Food quality is an issue of great concern in developing countries. Because many nutrients are obtained through food. Balado shrimp is a typical Padang dish with a spicy, sweet and savoury taste that is widely admired by all ages from children to adults. The presence of pathogenic bacteria, one of which is Salmonella sp, which contaminates balado shrimp food can cause health problems in the human body until death. The purpose of this study was to determine the presence or absence of Salmonella sp bacteria in processed shrimp balado sold in food stalls in the Mulyosari area. Method: In this study use as many as 30 samples of shrimp balado sold in food stalls Mulyosari area. This sample examination uses a streaking plate procedure and IMViC test. Data analysis using descriptive qualitative. Result: The results of this study showed that there were 3 samples identified Salmonella sp. Of the 30 samples, there was a range of 10% contaminated with Salmonella sp bacteria and 90% were not contaminated with Salmonella sp bacteria. Conclusion: This indicates that positive samples of balado shrimp must be further examined to determine the feasibility of consumption.
Perbedaan Frekuensi Aplikasi Handsanitizer Terhadap Jumlah Total Bakteri Telapak Tangan Artanti, Dita; Eka Sispita Sari, Yeti; Vita Purwaningsih, Nur; Riesti Retno Arimurti, Anindita; Azizah, Fitrotin; Rohmayani, Vella
Camellia : Clinical, Pharmaceutical, Analytical and Pharmacy Community Journal Vol 3 No 1 (2024): Camellia (Clinical, Pharmaceutical, Analytical, and Pharmacy Community Journal)
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/cam.v3i1.22920

Abstract

Virus Corona 2019, juga dikenal sebagai COVID-19, menyebar antar manusia melalui droplet atau cairan yang dikeluarkan saat batuk dan bersin dan menempel pada hal-hal di sekitarnya. Pandemi ini telah menjadi pandemi di seluruh dunia. Oleh karena itu, untuk mencegah penyebaran virus tersebut, setiap orang sekarang menjadi kebiasaan menggunakan sabun tangan. Hand sanitizer adalah antiseptik yang mengandung etil alkohol antara 60 dan 95 persen, dengan alkohol 70% yang paling umum digunakan. Tujuan penelitian ini adalah untuk mengetahui pengaruh frekuensi aplikasi cuci tangan menggunakan hand sanitizer terhadap jumlah total bakteri pada telapak tangan. Jenis penelitian ini adalah deskriptif. Populasi dalam penelitian ini adalah total bakteri pada telapak tangan yang diperiksa di Balai Besar Laboratorium Kesehatan Surabaya dengan sampel yang diambil sebanyak 21 swab telapak tangan dan dibagi menjadi 4 perlakuan (30 menit, 1 jam, 2 jam, dan 3 jam) dengan 1 kontrol. Hasil penelitian menunjukkan waktu aplikasi 30 menit pertumbuhan bakteri sebesar 970000 CFU, 1 jam sebesar 1410000 CFU, 2 jam sebesar 4788 CFU, 3 jam sebesar 18002 CFU. Waktu semprot yang paling efektif adalah penggunaan hand sanitizer dengan waktu penyemprotan yaitu 2 jam sekali dengan hasil 4786 CFU. Data yang diperoleh dianalisis menggunakan uji kruskall-wellis hasil probabilitas menunjukkan nilai signifikansi ρ= 0.754. Jadi terdapat pengaruh yang signifikan dalam pengaruh frekuensi aplikasi cuci tangan menggunakan hand sanitizer terhadap total bakteri pada telapak tangan.
Identifikasi Bakteri Makanan Ringan Kiloan Yang Dijual Di Pasar Tradisional Dengan Metode Angka Lempeng Total Sispita Sari, Yeti Eka; Azizah, Fitrotin; Retno Arimurti, Anindita Riesti; Artanti, Dita; Rohmayani, Vella; Ainutajriani, Ainutajriani; Saputro, Tri Ade
Indonesian Academia Health Sciences Journal Vol 4 No 1 (2023): Indonesian Academia Health Sciences Journal
Publisher : Universitas Muhammadiyah Surabaya

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Abstract

The rise in food poisoning incidents lately has made researchers want to know if there is bacterial contamination in kilo snacks which are always the top choice for serving and serving at certain events such as celebrations, recitations, thanksgiving, weddings, and other events because the prices are cheaper and the taste is better. not inferior to packaged snacks. Many places selling kilo snacks are found in traditional markets which generally have low environmental conditions of air circulation and high humidity, so they can become breeding grounds for harmful microbes, one of which is bacteria that can pollute the environment and reduce the cleanliness of indoor air. The aim is to find out the bacterial contamination of kilo snacks sold in traditional markets. The population in this study was a total of 5 types of kilo snacks in traditional markets. The sample in this study were 30 samples of 5 types of kilo snacks were tested using the Total Plate Number method. No samples of kilo snacks were found that did not meet the Indonesian National Standard (SNI). Kilo snacks sold in traditional markets meet the requirements of the Indonesian National Standard (SNI) Number 7388 of 2009
Aktivitas Lalat Rumah (Musca domestica) pada Pemberian Ekstrak Daun Pepaya (Carica papaya) daesusi, ruspeni; Anindita Riesti Retno Arimurti; Dita Artanti; Fitrotin Azizah; Novaulia Kinasih; Sispita Sari , yeti Eka
PEDAGO BIOLOGI Vol 12 No 1 (2024): April
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/pbjppb.v12i1.22677

Abstract

ABSTRACT House flies (Musca domestica) are vectors or mechanical transmitters of various infectious diseases through their body parts. The use of chemical insecticides to control houseflies has harmful effects on non-target insects, as well as exposure to humans and the environment. The use of insecticides made from natural ingredients is a solution to overcome the use of synthetic chemical insecticides. Papaya (Carica papaya) is a plant that is easy to grow and contains flavonoids, tannins, alkaloids, saponins, papain, calpain, and a number of other secondary metabolic compounds that are anti-mycoorganisms and parasites. This type of experimental research with a post test only group design aims to test the effect of papaya leaf extract on house fly activity. There are 3 treatments, namely P1 (papaya leaf extract), P2 (aquades) and P3 (branded liquid mosquito repellent). Each treatment was repeated 6 times. The research sample was 104 house flies. The results of the Mann Whitney test showed that there was a very significant difference (p<0.01) in the number (presentation) of houseflies that experienced changes in normal activity to abnormal ones between the administration of papaya leaf extract (72%) and the negative control using distilled water (0%). Meanwhile, administering branded liquid mosquito repellent provides 100% of the effect of flies experiencing abnormal activity. Thus, papaya leaf extract has been proven to have potential as a house fly repellent, although the effect is not as great as branded liquid mosquito repellent (p<0.05). Key words: Carica papaya, leaf extract, Musca domestica
DNA ISOLATION FROM HUMAN URINE STAIN AS AN ALTERNATIVE MATERIAL FOR PERSONAL IDENTIFICATION EXAMINATION Yudianto, Ahmad; Sispitasari, Yeti Eka
Folia Medica Indonesiana Vol. 52 No. 4 (2016): OCTOBER - DECEMBER 2016
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (192.388 KB) | DOI: 10.20473/fmi.v52i4.5476

Abstract

Accurate determination of personal identity is crucial for an investigation since any inaccuracy may lead to fatal consequences in the judicial process. Identification through DNA analysis involves somatic chromosomes and mtDNA. Each part of the human body can be taken as a specimen since every nucleated cell in the body of an individual has identical DNA sequence. To date, samples for identification through DNA analysis are obtained from blood stains, semen stains, bones, vaginal swab, buccal swab etc. In certain cases, urine stains on the clothing have frequently been overlooked. So far, personal identification through DNA analysis by the use of urine stains has not been commonly carried out. The present study detected bands in the loci CSF1PO, THO1, TPOX and 106bp-112bp amelogenin in all samples visualized from the results of Polymerase Chain Reaction (PCR) with Polyacrylamid Agarose Gel Electrophoreses-silver staining for exposure durations of 1, 7 and 14 days. However, for exposure duration of 20 days (the maximum in the study), bands were only detected in the loci THO1 and TPOX in all samples (100%), whereas the loci CSF1PO and 50% amelogenin exhibited obvious bands. This indicated that DNA analysis of urine stains through detection of the locus STR CSF1PO, THO1, TPOX exhibited different detection responses for different exposure durations assigned to the samples of urine stain. Successful detection of these loci was supported by the differences in amplicon product and GC content at each locus. Of the loci studied, the ratio of GC content of the primers, sorted from the lowest, were as follows: locus CSF1PO of 42.6 1%, TPOX of 56.25%, and THO1 of 63.83%. In conclusion, the loci THO1 and TPOX had the same probability of success in the STR examination compared with the locus CSF1PO.
EDUKASI DAN PELAYANAN KARYAWAN DI UNIVERSITAS MUHAMMADIYAH SURABAYA MELALUI KONSUMSI BAKTERI ASAM LAKTAT UNTUK PENINGKATAN SISTEM IMUN DI COVID-19 Artanti, Dita; Purwaningsih, Nur Vita; Sari, Yeti Eka Sispita; Azizah, Fitrotin; Samsudin, Rinza Rahmawati; Rohmayani, Vella; Arimurti, Anindita Riesti Retno; Juniawan, Mulya Fitrah
Humanism : Jurnal Pengabdian Masyarakat Vol 3 No 2 (2022): Agustus
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/hm.v3i2.14354

Abstract

Pada tahun 2020 seluruh dunia sedang mengalami kondisi pandemik akibat infeksi virus. Virus SARS-CoV-2 yang dapat menyebabkan penyakit COVID-19. Kondisi ini mengakibatkan “Panic Buying”, karena semua orang mencoba untuk menghindari penyebaran dan penularan penyakit COVID-19 dengan melakukan berbagai cara, salah satunya dengan mengambil tambahan suplemen seperti vitamin C dan suplemen probiotik. Probiotik merupakan kelompok bakteri asam laktat, jika dikonsumsi dalam jumlah yang cukup dapat memberikan manfaat kesehatan bagi tubuh. Bakteri asam laktat memiliki fungsi sebagai imunomodulator atau meningkatkan sistem kekebalan tubuh sehingga dapat mencegah infeksi penyakit pada hewan dan manusia. Kegiatan edukasi dan pelayanan dilakukan melalui poster dan penyampaian langsung dengan metode door to door. Sehingga karyawan di Universitas Muhammadiyah Surabaya lebih mudah memahami terkait konsumsi bakteri asam laktat untuk peningkatan sistem imun di COVID-19. Para karyawan sangat antusias dengan kegiatan edukasi yang dilakukan, mereka juga menjadi lebih disiplis untuk menghindari penularan dan pencegahan penyakit COVID-19.Kata Kunci: edukasi dan pelayanan karyawan, COVID-19, Bakteri Asam Laktat, dan sistem imun. 
Sertifikasi halal untuk peningkatan pelayanan unggul suplaier gizi unit poned puskesmas Anas, Muhammad; Latifah, Luluk; Saputro, Andre Ridho; Sari, Yeti Eka Sispita; Siswanto, Alfiansya Noval; Jamil, Ahmad Mochtar
Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Vol. 4 No. 3 (2023)
Publisher : Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jp2m.v4i3.20897

Abstract

Pemerintah melalui Badan Penyelenggara Jaminan Produk Halal (BPJPH) mewajibkan sertifikasi halal baik untuk produk maupun jasa. Puskesmas dengan pelayanan rawat inap pelayanan obstetri neonatus emergensi dasar (PONED) di puskesmas Kedundung terdapat suplaier gizi. Suplaier Gizi Puskesmas Kedundung saat ini belum tersertifikasi halal. Kondisi tersebut yang dimanfaatkan oleh Tim Pengabdi untuk memfasilitasi para suplaier gizi dalam meningkatkan kesadaran akan pentingnya kaidah halal pada produk-produknya melalui sosialisasi dan pendampingan sertifikasi halal. Sertifikasi halal produk unit gizi dilakukan dengan sosialisasi halal, pelatihan system jaminan produk halal (SJPH), komitmen halal, dan pendampingan sertifikasi halal. Sertifikasi halal produk gizi unit PONED Puskesmas Kedundung berfungsi sebagai salah satu sarana untuk meningkatkan nilai tambah pelayanan yang dihasilkan, serta memberikan ketenangan bagi pasien, terutama bagi pasien Muslim. Selain itu, para suplaier gizi ditantang untuk menerapkan Sistem Jaminan Halal secara berkesinambungan sehingga mutu dan kualitas pelayanan bahkan integritas suatu unit pelayanan menjadi lebih baik di mata pasien.
Co-Authors Adelia Septiyandari Ahmad Yudianto Ainutajriani, Ainutajriani Andre Ridho Saputro Anindita Riesti Retno Arimurti A`im Matun Nadhiroh Baterun Kunsah Diah Ariana Dita Artanti DITA ARTANTI Ellies Tunjung Sari Maulidiyanti Ellies Tunjung Sari Maulidiyanti Fitrotin Azizah Fitrotin Azizah, Fitrotin Jamil, Ahmad Mochtar Lihabi, Libahi Luluk Latifah Muhammad Anas Mulya Fitrah Juniawan Musrifatul Uliyah Mustofa, Nabila Amelia Nastiti Kartikorini Nastiti Kartikorini, Nastiti Novaulia Kinasih Nur Hidayatullah Romadhon Nur Vita Purwaningsih Nur Vita Purwaningsih, Nur Vita Rinza Rahmawati Samsudin Rohmayani, Vella Rohmayani, Vella Rohmayani, Ruspeni Daesusi Saputro, Tri Ade Sembiring, Rinawati Siswanto, Alfiansya Noval Siti Aisyah Vella Rohmayani Vita Purwaningsih, Nur