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Perbedaan Frekuensi Aplikasi Handsanitizer Terhadap Jumlah Total Bakteri Telapak Tangan Artanti, Dita; Eka Sispita Sari, Yeti; Vita Purwaningsih, Nur; Riesti Retno Arimurti, Anindita; Azizah, Fitrotin; Rohmayani, Vella
Camellia : Clinical, Pharmaceutical, Analytical and Pharmacy Community Journal Vol 3 No 1 (2024): Camellia (Clinical, Pharmaceutical, Analytical, and Pharmacy Community Journal)
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/cam.v3i1.22920

Abstract

Virus Corona 2019, juga dikenal sebagai COVID-19, menyebar antar manusia melalui droplet atau cairan yang dikeluarkan saat batuk dan bersin dan menempel pada hal-hal di sekitarnya. Pandemi ini telah menjadi pandemi di seluruh dunia. Oleh karena itu, untuk mencegah penyebaran virus tersebut, setiap orang sekarang menjadi kebiasaan menggunakan sabun tangan. Hand sanitizer adalah antiseptik yang mengandung etil alkohol antara 60 dan 95 persen, dengan alkohol 70% yang paling umum digunakan. Tujuan penelitian ini adalah untuk mengetahui pengaruh frekuensi aplikasi cuci tangan menggunakan hand sanitizer terhadap jumlah total bakteri pada telapak tangan. Jenis penelitian ini adalah deskriptif. Populasi dalam penelitian ini adalah total bakteri pada telapak tangan yang diperiksa di Balai Besar Laboratorium Kesehatan Surabaya dengan sampel yang diambil sebanyak 21 swab telapak tangan dan dibagi menjadi 4 perlakuan (30 menit, 1 jam, 2 jam, dan 3 jam) dengan 1 kontrol. Hasil penelitian menunjukkan waktu aplikasi 30 menit pertumbuhan bakteri sebesar 970000 CFU, 1 jam sebesar 1410000 CFU, 2 jam sebesar 4788 CFU, 3 jam sebesar 18002 CFU. Waktu semprot yang paling efektif adalah penggunaan hand sanitizer dengan waktu penyemprotan yaitu 2 jam sekali dengan hasil 4786 CFU. Data yang diperoleh dianalisis menggunakan uji kruskall-wellis hasil probabilitas menunjukkan nilai signifikansi ρ= 0.754. Jadi terdapat pengaruh yang signifikan dalam pengaruh frekuensi aplikasi cuci tangan menggunakan hand sanitizer terhadap total bakteri pada telapak tangan.
Gambaran Infeksi Soil Transmitted Helminth pada Petani Penyitas Erupsi Gunung Semeru Rohmayani, Vella Rohmayani,; Arimurti, Anindita Riesti Retno; Sari, Yeti eka Sispita; Romadhon, Nur Hidayatullah; Lihabi, Libahi
The Indonesian Journal of Infectious Diseases Vol. 9 No. 2 (2023): The Indonesian Journal of Infectious Disease
Publisher : Rumah Sakit Penyakit Infeksi Prof Dr. Sulianti Saroso

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32667/ijid.v9i2.172

Abstract

Latar belakang: Soil Transmitted Helminth (STH) adalah cacing golongan Nematoda usus yang siklus penularannya membutuhkan tanah. Tujuan: untuk mengetahui gambaran infeksi parasit STH pada kuku tangan petani penyintas erupsi gunung Semeru di desa Sumbermujur kecamatan Candipuro kabupaten Lumajang. Metode: Desain penelitian cross sectional. Sampel penelitian adalah petani penyintas erupsi semeru sebanyak 25 orang. Pengumpulan data menggunakan metode wawancara dan pemeriksaan potongan kuku tangan di laboratorium untuk mengidentifikasi keberadaan telur parasit STH pada petani penyintas erupsi gunung Semeru. Sampel yang digunakan adalah kuku tangan petani penyintas erupsi Gunung Semeru. Pemeriksaan sampel di laboratorium menggunakan metode sedimentasi. Hasil: dari 25 sampel yang diperiksa ditemukan sebanyak 4 sampel atau sebesar 16% yang dinyatakan positif terinfeksi STH dan 4 sampel yang dinyatakan positif ditemukan 1 jenis parasit STH yaitu spesies Ascaris lumbricoides. Berdasarkan hasil uji Chi Square diperoleh Pvalue=0,03. Kesimpulan: ada hubungan antara kebersihan diri dengan keberadaan telur STH pada sampel kuku tangan petani.
Identifikasi Bakteri Makanan Ringan Kiloan Yang Dijual Di Pasar Tradisional Dengan Metode Angka Lempeng Total Sispita Sari, Yeti Eka; Azizah, Fitrotin; Retno Arimurti, Anindita Riesti; Artanti, Dita; Rohmayani, Vella; Ainutajriani, Ainutajriani; Saputro, Tri Ade
Indonesian Academia Health Sciences Journal Vol 4 No 1 (2023): Indonesian Academia Health Sciences Journal
Publisher : Universitas Muhammadiyah Surabaya

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Abstract

The rise in food poisoning incidents lately has made researchers want to know if there is bacterial contamination in kilo snacks which are always the top choice for serving and serving at certain events such as celebrations, recitations, thanksgiving, weddings, and other events because the prices are cheaper and the taste is better. not inferior to packaged snacks. Many places selling kilo snacks are found in traditional markets which generally have low environmental conditions of air circulation and high humidity, so they can become breeding grounds for harmful microbes, one of which is bacteria that can pollute the environment and reduce the cleanliness of indoor air. The aim is to find out the bacterial contamination of kilo snacks sold in traditional markets. The population in this study was a total of 5 types of kilo snacks in traditional markets. The sample in this study were 30 samples of 5 types of kilo snacks were tested using the Total Plate Number method. No samples of kilo snacks were found that did not meet the Indonesian National Standard (SNI). Kilo snacks sold in traditional markets meet the requirements of the Indonesian National Standard (SNI) Number 7388 of 2009
Dna isolation from human urine stain as an alternative material for personal identification examination Yudianto, Ahmad; Sispitasari, Yeti Eka
Folia Medica Indonesiana Vol. 52, No. 4
Publisher : Folia Medica Indonesiana

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Abstract

Accurate determination of personal identity is crucial for an investigation since any inaccuracy may lead to fatal consequences in the judicial process. Identification through DNA analysis involves somatic chromosomes and mtDNA. Each part of the human body can be taken as a specimen since every nucleated cell in the body of an individual has identical DNA sequence. To date, samples for identification through DNA analysis are obtained from blood stains, semen stains, bones, vaginal swab, buccal swab etc. In certain cases, urine stains on the clothing have frequently been overlooked. So far, personal identification through DNA analysis by the use of urine stains has not been commonly carried out. The present study detected bands in the loci CSF1PO, THO1, TPOX and 106bp-112bp amelogenin in all samples visualized from the results of Polymerase Chain Reaction (PCR) with Polyacrylamid Agarose Gel Electrophoreses-silver staining for exposure durations of 1, 7 and 14 days. However, for exposure duration of 20 days (the maximum in the study), bands were only detected in the loci THO1 and TPOX in all samples (100%), whereas the loci CSF1PO and 50% amelogenin exhibited obvious bands. This indicated that DNA analysis of urine stains through detection of the locus STR CSF1PO, THO1, TPOX exhibited different detection responses for different exposure durations assigned to the samples of urine stain. Successful detection of these loci was supported by the differences in amplicon product and GC content at each locus. Of the loci studied, the ratio of GC content of the primers, sorted from the lowest, were as follows: locus CSF1PO of 42.6 1%, TPOX of 56.25%, and THO1 of 63.83%. In conclusion, the loci THO1 and TPOX had the same probability of success in the STR examination compared with the locus CSF1PO.