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All Journal The Indonesian Journal of Dental Research MKGK (Majalah Kedokteran Gigi Klinik) (Clinical Dental Journal) UGM Majalah Kedokteran Gigi Indonesia ODONTO Dental Journal Dental Journal (Majalah Kedokteran Gigi) The Indonesian Biomedical Journal B-Dent, Jurnal Kedokteran Gigi Universitas Baiturrahmah E-Prodenta Journal of Dentistry Odonto dental journal
Kwartarini Murdiastuti
Faculty Of Dentistry, Universitas Gadjah Mada, Yogyakarta
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Medicine & Pharmacology Public Health

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Antimicrobial effect of chlorine dioxide on Actinobacillus actinomycetemcomitans in diabetes mellitus rats treated with insulin Tantin Ermawati; Kwartarini Murdiastuti
Dental Journal (Majalah Kedokteran Gigi) Vol. 45 No. 1 (2012): March 2012
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (307.191 KB) | DOI: 10.20473/j.djmkg.v45.i1.p22-27

Abstract

Background: Periodontitis is a chronic inflammatory disease of periodontal tissues. Etiology of periodontal disease includes Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans) which is the most predominant disease-causing bacteria found in the gingival sulcus. Periodontitis can be exacerbated by the systemic disease, such as diabetes mellitus considered as a metabolic disease characterized by hyperglycemia due to insulin deficiency. Treatment of periodontitis is then required in patients with type I diabetes to avoid radical reaction that can not only cause bleeding, but can also prevent infection, as a result, topical antimicrobial therapy and blood glucose control are required. Topical antimicrobial chlorine dioxide is a disinfectant that is effective in killing A. actinomycetemcomitans. Purpose: This study is aimed to determine the effects of topical antimicrobial chlorine dioxide gel or rinse on the number of A. actinomycetemcomitans in DM rats treated with insulin. Methods: 20 three month old male Wistar rats with weight of 170–200 grams were divided into four groups. First, periodontitis and DM were manipulated into all groups through aloksan injection with dose of 170 mg/kg. Those rats in group I were treated with insulin and chlorine dioxide gel, those in group II were treated with insulin and chlorine dioxide rinse, those in group III were treated with insulin only, and those in group IV were without treatment. In the third and seventh weeks, the number of A. actinomycetemcomitans was measured. The data was tested by using One-Way ANOVA test followed by LSD test. Results: The study showed that chlorine dioxide gel has a greater ability in reducing the number of A. actinomycetemcomitans than chlorine dioxide rinse although both are antimicrobials. Conclusion: It can be concluded that the use of chlorine dioxide gel can more effective to decrease the number of A. actinomycetemcomitans than chlorine dioxide rinse in DM rats treated with insulin therapy.Latar belakang: Periodontitis adalah suatu penyakit inflamasi kronis jaringan periodontal. Etiologi penyakit periodontal diantaranya adalah A. actinomycetemcomitans yang merupakan bakteri paling dominan penyebab penyakit yang terdapat pada actinomycetemcomitans yang merupakan bakteri paling dominan penyebab penyakit yang terdapat pada sulkus gingiva. Periodontitis yang terjadi diperparah adanya penyakit sistemik yaitu diabetes mellitus (DM) yang merupakan penyakit metabolik yang ditandai dengan hiperglikemi akibat defisiensi insulin. Perawatan periodontitis pada penderita DM tipe I adalah untuk Perawatan periodontitis pada penderita DM tipe I adalah untuk menghindari tindakan radikal yang dapat menyebabkan perdarahan dan mencegah terjadinya infeksi, sehingga digunakan terapi antimikroba topikal serta kontrol glukosa darah. Antimikroba topikal chlorine dioxide merupakan desinfektan yang efektif dalam membunuh A. actinomycetemcomitans. Tujuan: Penelitian ini bertujuan untuk mengetahui efek antimikroba topikal chlorine dioxide gel atau rinse terhadap jumlah A. actinomycetemcomitans pada tikus DM dengan insulin. Metode: Dua puluh ekor tikus Wistar, jantan, usia 3 bulan, berat 170–200 gram, dibagi menjadi empat kelompok. Semua kelompok sebelumnya dimanipulasi periodontitis serta DM dengan injeksi aloksan dosis 170 mg/kgBB. Kelompok I adalah tikus yang diterapi insulin dan chlorine dioxide gel, kelompok II diterapi insulin dan chlorine dioxide rinse, kelompok III diterapi insulin, serta kelompok IV adalah tikus tanpa dilakukan terapi apapun. Pada minggu ke-3 dan ke-7 dilakukan penghitungan jumlah A. actinomycetemcomitans. Hasil pengukuran di analisis menggunakan uji Anava 1 jalur dilanjutkan dengan uji LSD. Hasil: Penelitian menunjukkan bahwa chlorine dioxide gel mempunyai kemampuan lebih besar dalam menurunkan jumlah A. actinomycetemcomitans dibandingkan chlorine dioxide rinse, walaupun keduanya bersifat antimikroba. Kesimpulan: Dapat disimpulkan bahwa pemberian chlorine dioxide gel lebih efektif menurunkan jumlah A. actinomycetemcomitans dibandingkan kelompok yang diberi chlorine dioxide rinse pada tikus DM dengan terapi insulin.
Differences in mucin expression in the submandibular glands of rats during peridontitis induction Nunuk Purwanti; Banun Kusumawardhani; Kwartarini Murdiastuti
Dental Journal (Majalah Kedokteran Gigi) Vol. 51 No. 2 (2018): June 2018
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v51.i2.p52-56

Abstract

Background: Porphyromonas gingivalis (Pg) produces lipopolysacharide (LPS) which acts as a stimulator of inflammation in periodontal tissues. Periodontitis-induced apoptosis and vacuolation of the salivary gland, therefore, causes hyposalivation. Mucin secretion is produced by the submandibular gland under stimulation by the cholinergic and adrenergic receptors. Both forms of stimulation influence the volume of mucin secretion. Mucin saliva plays an important role in the early stages of Pg colonization in the oral cavity. On the other hand, it serves to protect against bacterial invasion. Purpose: The aim of this research was to identify differences in mucin expression in the submandibular gland during periodontitis induction. Methods: 32 male Wistar rats were assigned to either a sham periodontitis or a periodontitis group. The former group received a daily injection of a vehicle solution (n = 16), while members of the periodontitis induction group (n=16) were injected each day with 500 µL of Pg 108 into the mesial area of the upper molar. Mucin in the submandibular gland was analyzed at the 7th, 14th, 21th and 28th days after injection by means of periodic acid schiff (PAS) staining. Results: 28 days after injection mild gingivitis was developed in the periodontitis experiment group. Junctional epithelium (JE) thickness decreased gradually following the increase of PG injection periods (p<0.05).  However, mucin expression increased prominently at 7th, 14th, and 21th days after injection and decreased on day 28th after PG injection. Mucin was expressed in the duct cells of the submandibular gland. Conclusion: The result of this study suggests that there are different levels of mucin expression in the submandibular gland during periodontitis induction.
The Effect of Collagen Activation on Platelet Rich Plasma for Proliferation of Periodontal Ligament Fibroblasts Pati Tangsupati; Kwartarini Murdiastuti
The Indonesian Biomedical Journal Vol 10, No 3 (2018)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v10i3.432

Abstract

BACKGROUND: Regenerative procedure in periodontal surgery aims to improve the structure and function of the periodontium to be strong enough to support the teeth. Growth factor is essential in the process of tissue regeneration; it can be generated from the activation of Platelet-Rich Plasma (PRP). In this study, collagen was used as PRP activator. PRP release growth factor from the granules when activated. The aim of this study was to evaluate the effect of collagen on PRP activation and the collagen-activated PRP storage to fibroblast proliferation of periodontal ligament (PDL).METHODS: Fibroblasts of PDL were obtained from extracted premolar. PRP were obtained from 100 mL of blood donors using double centrifugation methods. PRP were activated by collagen and subsequently incubated for 24, 48, 72 and 168 hours; after which the lysate was taken. Fibroblasts were divided into 7 groups, which consisted of one unstimulated group as negative control, one group stimulated by PRP lysate, and five groups stimulated with PRP-collagen lysates that had been incubated for 24, 48, 72 and 168 hours. MTT assay was then performed after 1 and 3 days.RESULTS: The proliferation rate of fibroblasts group stimulated by PRP-collagen lysate was higher than the group stimulated by PRP. The storage of collagen-activated PRP for 7 days at 4°C could increase the proliferation rate.CONCLUSION: The activation of collagen on PRP and the stored collaged-activated PRP could increase the fibroblasts proliferation rate of PDL.KEYWORDS: collagen, platelet-rich plasma, fibroblasts proliferation
Effect of advanced-platelet rich-fibrin combined with rosuvastatin application after open flap debridement of infrabony pocket Tissa Rahadianti; Dahlia Herawati; Kwartarini Murdiastuti
Majalah Kedokteran Gigi Indonesia Vol 7, No 2 (2021): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.53419

Abstract

Open flap debridement (OFD) is an invasive therapy for chronic periodontitis with pocket 5 mm or more. However, it is difficult to achieve regeneration and new attachment with this therapy. Periodontitis starts to add growth factors and local drugs delivery as host modulation therapy. Advanced-PRF (A-PRF) contains more growth factor than PRF which plays a role in promoting fibroblast proliferation, reepithelization, extracellular matrix production, and endothelial cell migration. 1.2% rosuvastatin gel (RSV) is a local delivery drug with a pleiotropic effect that can modify host response to promoting BMSCs, BMP-2, OPG, ALP, RANKL, and osteoblasts. This study aimed to examine the effect of the application of A-PRF+RSV in OFD therapy of which the parameters were probing depth (PD), relative attachment loss (RAL), and alveolar bone height. The study samples consisted of 24 periodontal pockets which were divided into 2 groups of 12 pockets each, namely A-PRF+RSV for group 1 and PRF+RSV for group 2. Clinical evaluations were carried out on baseline, day-30, and day-90 for PD and RAL, and on baseline and day -90 for alveolar bone height. Data of PD and RAL reduction were analyzed with non-parametric test Mann-Withney, while data of reduction of alveolar bone height were analyzed with parametric Independent-T test. Group 1 obtained a statistically more significant result in reducing PD, RAL, and alveolar bone height compared to group 2 (p<0.05) To conclude, the application of A-PRF and 1.2% rosuvastatin gel in OFD procedure promotes a higher PD and RAL reduction and alveolar bone height increase than the application of PRF coupled with 1.2% rosuvastatin gel.
EFEKTIVITAS FLEP POSISI KORONAL DENGAN DAN TANPA ACELLULAR DERMAL MATRIX ALLOGRAFT PADA RESESI GINGIVA Citra Lestari; Kwartarini Murdiastuti; Ahmad Syaify
B-Dent: Jurnal Kedokteran Gigi Universitas Baiturrahmah Volume 1, Nomor 1, Juni 2014
Publisher : Universitas Baiturrahmah

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (305.332 KB) | DOI: 10.33854/JBDjbd.52

Abstract

Resesi gingiva adalah terbukanya akar gigi akibat pergeseran posisi margin gingiva ke apikal dari cemento enamel junction. Salah satu perawatan resesi gingiva adalah flep posisi koronal (FPK) yang dapat dilakukan dengan dan tanpa acellular dermal matrix allograft (ADMA). Acellular dermal matrix allograft merupakan jaringan kulit yang diambil dari individu lain yang diproses khusus sehingga sel-sel dermal terangkat dengan tetap mempertahankan matriks kolagen regeneratif. Kandungan utama ADMA terdiri dari kolagen, elastin, proteoglikan dan pembuluh darah yang berguna mengarahkan regenerasi dan reparasi jaringan periodontal. Penelitian ini bertujuan untuk mengetahui efektivitas flep posisi koronal dengan dan tanpa acellular dermal matrix allograft pada perawatan resesi gingiva dilihat dari parameter gingival thickness (GT) yang dinilai dari dua daerah yaitu ketebalan gingiva keratin (GT1) dan ketebalan mukosa alveolar (GT2). Penelitian dilakukan terhadap 20 kasus resesi gingiva dengan melihat pengukuran GT1 dan GT2 dilakukan sebelum dan 3 bulan sesudah operasi. Hasil analisis statistik unpaired t-test menunjukkan adanya perbedaan peningkatan GT1 dan GT2 yang bermakna (p
The difference in scaling root-planing results between addition of photodynamic therapy and application of metronidazole gel of 25% in chronic periodontitis treatment Andreas Tjandra; Kwartarini Murdiastuti; Al Sri Koes Soesilowati; Fitri Yuniawati
Majalah Kedokteran Gigi Indonesia Vol 7, No 3 (2021): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.54560

Abstract

Scaling Root-Planing (SRP) is a mechanical treatment for removing hard and soft deposits as well as bacteria that adhere to the tooth surface. The additional use of Photodynamic Therapy (PDT) can lead to damage of pathogen bacteria cell. Metronidazole is a common antibiotic with broad spectrum that goes againsts periodontal pathogen. This study aimed to compare the treatment outcomes between combined treatment SRP+PDT and SRP+metronidazole gel 25% in treating chronic periodontitis from the clinical parameters of Pocket Depth (PD), Relative Attachment Level (RAL), Papillary Bleeding Index (PBI) and the number of bacterial colonies. The study samples were taken from 30 periodontal pocket points, which were divided into two treatment groups. The first group was treated with SRP, then had an application of chlorophyll into the pockets and was lighted by 405 nm light, while the second group was treated with SRP, and was applied by metronidazole gel 25% into the pocket. The procedures were started by taking bacteria in the pocket using paper point in two treatment groups, then measuring PD, RAL and PBI as the baseline. After the treatments, PD and RAL results were calculated within observation time of the baseline, the 3rd, the 6th and the 9th weeks, while PBI and the number of bacterial colony were calculated at the baseline and the 3rd week. Distribution of data was analyzed by Shapiro Wilk test and the data reduction used non parametric test of Mann Whitney. The result showed a decreasing level in PD, RAL, PBI and the number of bacterial colony in both groups, but significant difference was seen in PD, and RAL of the baseline of the 9th week. Thus, the study concludes that the combination of SRP+PDT has the same effectiveness when compared to the combination of SRP+metronidazole gel of 25% in chronic periodontitis treatment, and the result is even greater in reducing the level of PD and RAL as seen from the baseline of 9th week measurement.
Effect of the application of curcumin (Curcuma Longa) oral gel on periodontal inflammation in patients with type 2 diabetes melitus Nur Rahman Ahmad Seno Aji; Vincensia Maria Karina; Osa Amila Hafiyyah; Kwartarini Murdiastuti; Ahmad Syaify
Majalah Kedokteran Gigi Indonesia Vol 7, No 3 (2021): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.69036

Abstract

Patients with Type 2 Diabetes mellitus (T2DM) were more prone to suffer from periodontitis due to unique alteration in host immune response. Application of curcumin oral gel as adjunctive therapy was expected to improve periodontal condition due to its anti-inflammatory properties. The objective of this study was to evaluate the effect of curcumin oral gel on periodontal inflammation in patients with T2DM. Sixteen periodontal pockets (n=16) from patients with T2DM were administered with curcumin oral gel using blunt cannula after periodontal curettage. Gingival Index (GI) and Periodontal Pocket Probing Depth (PPD) were evaluated a month after application. GI was assessed using visible sign of inflammation parameters including swelling, redness and bleeding upon probing. PPD was assessed using UNC-15 periodontal probe. The results were analyzed by Wilcoxon test (two related samples). The results showed that GI before treatment decreased by 51.61% a month post application. There was statistically significant difference (Sig 0.003) between GI mean before and after curcumin application. PPD decreased by 42.81% and showed statistically significant difference (Sig 0.000) by Wilcoxon Test. It is concluded that application of curcumin oral gel post curettage could decrease periodontal inflammation in patients with T2DM.
EFFECT OF THE ACTIVATION TIME OF PLATELET RICH PLASMA AND COLLAGEN ON THE LEVELS OF FIBROBLAST GROWTH FACTOR IN GINGIVAL CREVICULAR FLUID Adisty Restu Poetri; Kwartarini Murdiastuti; Sudibyo sudibyo
Odonto : Dental Journal Vol 9: Special Issue 1. April 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (317.491 KB) | DOI: 10.30659/odj.9.0.162-169

Abstract

Background: Gingival recession is a condition that occurs when the gingival margin is more apically than the cemento-enamel junction and causes the tooth root to open, so a root closure procedure is required. Platelet rich plasma (PRP) contains growth factors, including Fibroblast growth factor which plays a role in the process of angiogenesis and cell proliferation. The activation time is known to affect platelet count and FGF expression. The purpose of this study was to determine the effect of the activation time of platelet rich plasma and collagen on the expression of fibroblast growth factor in the gingival crevicular fluidMethod: The research sample was gingival crevicular fluid which was taken using paper point #30 for 30 seconds as many as 18 samples. Samples were taken before surgery, day 5 and day 7 after surgery. The samples were divided into 2 groups based on the activation time, 1 hour and 24 hours PRP-collagen. Changes in FGF levels were examined using ELISA. Repeated Meassure ANOVA test was conducted to compare the differences in each time observation and post hoc was performed to compare the differences between groups for each time observation.Result: There was no significant difference in FGF levels in the gingival crevicular fluid at each time and between groups.Conclusion: There was no effect of the activation time of platelet rich plasma and collagen on the levels of fibroblast growth factor in gingival crevicular fluid
THE INFLUENCE BETWEEN INJECTABLE PLATELET-RICH FIBRIN AND PLATELET-RICH PLASMA TOWARDS GINGIVAL FIBROBLAST CELL PROLIFERATION Arifia Anindita Danastri; Suryono Suryono; Kwartarini Murdiastuti
Odonto : Dental Journal Vol 8, No 2 (2021): December 2021
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (287.186 KB) | DOI: 10.30659/odj.8.2.25-31

Abstract

ABSTRACTBackground: Gingiva is the outermost periodontal tissue that acts as a mechanical and biological barrier to the root of the teeth and alveolar bone. The main cellular elements in the gingiva are fibroblasts. Fibroblast cell proliferation is an important process in tissue regeneration. Growth factors that can stimulate fibroblast cell proliferation can be found in regenerative agents, such as injectable platelet-rich fibrin (i-PRF) and platelet-rich plasma (PRP). The aim of this study was to examine the influence between i-PRF and PRP on the gingival fibroblast cell proliferation in vitro study on primary cell culture.Method: Gingival fibroblast cell were obtained from primary cell culture derived from healthy gingiva. Ten mL of peripheral blood were centrifuged for i-PRF and PRP preparation. The samples were divided into three groups: i-PRF, PRP, and fibroblast cells without treatment. Cell proliferation were observed at day 1, day 3, day 5 using MTT assay at 550 nm. The data were analyzed by Two-Way ANOVA test, followed by Post Hoc test.Result: The results showed that the cell proliferation increased from day 1, 3, and 5 in all groups. The absorbance value of the cell proliferation in order from highest to lowest: i-PRF, PRP, and cell control.Conclusion: i-PRF and PRP increased the gingival fibroblast cell proliferation. i-PRF increased the cell proliferation higher than PRP.
MANAGEMENT OF ABERRANT FRENUM LABII SUPERIOR WITH FRENECTOMY USING Z PLASTY TECHNIQUE Noormaya Sari; Murdiastuti, Kwartarini
E-Prodenta Journal of Dentistry Vol 8 No 2 (2024)
Publisher : Fakultas Kedokteran Gigi UB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.eprodenta.2024.008.02.8

Abstract

Latar Belakang: Midline diastema pada gigi anterior maksila yang disebabkan oleh frenum labii superior dengan perlekatan yang tinggi dapat menyebabkan masalah estetis sehingga mempengaruhi kepercayaan diri seseorang. Pemeriksaan menyeluruh dan seleksi kasus penting dalam menentukan tindakan yang tepat dalam menangani masalah tersebut. Tujuan: Laporan kasus ini bertujuan untuk memberikan gambaran penanganan aberrant frenum labii superior dengan frenektomi menggunakan teknik Z plasty. Kasus: Seorang pasien wanita berumur 25 tahun datang ke Klinik Periodonsia RSGM Prof. Soedomo mengeluhkan gigi depan rahang atasnya yang terlihat renggang dan bercelah sehingga mempengaruhi kepercayaan diri pasien. Hasil pemeriksaan klinis intaoral tampak frenum labii superior yang hipertrofi, tebal, lebar disertai vestibulum yang dangkal dengan hasil pemeriksaan blanch test positif. Penatalaksanaan: Frenektomi dilakukan dengan teknik Z plasty menggunakan scalpel, membentuk flap triangular sama besar pada sisi kiri dan kanan yang kemudian ditransposisi. Flap kemudian dijahit dan selanjutnya  ditutup dengan dressing periodontal. Kontrol 1 minggu, 2 minggu, 1 bulan dan 3 bulan pasca operasi menunjukkan hasil yang memuaskan. Aberrant frenum dapat terkoreksi dengan baik, frenum tidak relapse, tidak terbentuk jaringan parut, lebar gingiva cekat maupun kedalaman vestibulum meningkat. Kesimpulan: Manajemen frenum yang hipertrofi dengan perlekatan tinggi disertai vestibulum dangkal yang dilakukan dengan tindakan frenektomi menggunakan teknik Z plasty terbukti memberikan hasil yang memuaskan baik secara estetik maupun fungsional.
Co-Authors Adisty Restu Poetri Ahmad Syaify Ahmad Syaify, Ahmad Aini Moeljono Al Sri Koes Soesilowati Andreas Tjandra Angga Febriharta Anton Kusumo Widagdo Arifia Anindita Danastri Banun Kusumawardhani Citra Lestari Dahlia Herawati Febriharta, Angga Fitri Yuniawati Fitri Yuniawati Flandiana Yogianti Hendry Dwi Wijayanto Mefi Priba Sari Noormaya Sari Nunuk Purwanti Nur Rahman Ahmad Seno Aji Nurbaiti, Mentari Salma Oktarina, Dyah Ayu Mira Osa Amila Hafiyyah Pati Tangsupati Rani Gamawati Sri Pramestri Lastianny, Sri Pramestri Sudibyo sudibyo Suryono Suryono Suryono Suryono Tantin Ermawati Tissa Rahadianti Tutut Prabantari Anindyajati Vincensia Maria Karina