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All Journal The Indonesian Journal of Dental Research MKGK (Majalah Kedokteran Gigi Klinik) (Clinical Dental Journal) UGM Majalah Kedokteran Gigi Indonesia ODONTO Dental Journal Dental Journal (Majalah Kedokteran Gigi) The Indonesian Biomedical Journal Padjadjaran Journal of Dentistry B-Dent, Jurnal Kedokteran Gigi Universitas Baiturrahmah E-Prodenta Journal of Dentistry Odonto dental journal
Kwartarini Murdiastuti
Faculty Of Dentistry, Universitas Gadjah Mada, Yogyakarta
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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THE INFLUENCE BETWEEN INJECTABLE PLATELET-RICH FIBRIN AND PLATELET-RICH PLASMA TOWARDS GINGIVAL FIBROBLAST CELL PROLIFERATION Danastri, Arifia Anindita; Suryono, Suryono; Murdiastuti, Kwartarini
Odonto : Dental Journal Vol 8, No 2 (2021): December 2021
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.8.2.25-31

Abstract

ABSTRACTBackground: Gingiva is the outermost periodontal tissue that acts as a mechanical and biological barrier to the root of the teeth and alveolar bone. The main cellular elements in the gingiva are fibroblasts. Fibroblast cell proliferation is an important process in tissue regeneration. Growth factors that can stimulate fibroblast cell proliferation can be found in regenerative agents, such as injectable platelet-rich fibrin (i-PRF) and platelet-rich plasma (PRP). The aim of this study was to examine the influence between i-PRF and PRP on the gingival fibroblast cell proliferation in vitro study on primary cell culture.Method: Gingival fibroblast cell were obtained from primary cell culture derived from healthy gingiva. Ten mL of peripheral blood were centrifuged for i-PRF and PRP preparation. The samples were divided into three groups: i-PRF, PRP, and fibroblast cells without treatment. Cell proliferation were observed at day 1, day 3, day 5 using MTT assay at 550 nm. The data were analyzed by Two-Way ANOVA test, followed by Post Hoc test.Result: The results showed that the cell proliferation increased from day 1, 3, and 5 in all groups. The absorbance value of the cell proliferation in order from highest to lowest: i-PRF, PRP, and cell control.Conclusion: i-PRF and PRP increased the gingival fibroblast cell proliferation. i-PRF increased the cell proliferation higher than PRP.
Effect of sandblasted large grit acid etching and ultraviolet-c photofunctionalization of titanium on osteoblast cell viability: an in vitro study Tjandra, Andreas; Murdiastuti, Kwartarini; Kusumaatmaja, Ahmad; Susilowati, Heni
Padjadjaran Journal of Dentistry Vol 38, No 2 (2026): July 2026
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol38no2.70560

Abstract

Introduction: Sandblasted Large Grit Acid Etching (SLA) is a widely used titanium implant surface modification technique that enhances osseointegration. Ultraviolet C (UV-C) photofunctionalization is a method that modifies the physicochemical properties of titanium surfaces by removing hydrocarbon contaminants and increasing hydrophilicity. However, the combined effects of these modification techniques on MC3T3-E1 osteoblast cell viability require further investigation. Therefore, this study aimed to analyze the effect of SLA treatment and its interaction with UV-C irradiation on MC3T3-E1 osteoblast cell viability on titanium disks. Methods: This in vitro experimental study used titanium disks (10 mm in diameter and 1 mm in thickness), which were divided into SLA-treated and non-SLA groups. Each group was further subdivided according to UV-C irradiation (90 W, 254 nm) into four subgroups: non-UV (control), 10 minutes, 30 minutes, and 48 hours (n = 4 per subgroup). MC3T3-E1 osteoblasts (1.2×104 cells/well) were seeded onto disk surfaces and cultured for 24 hours. Cell viability was assessed using the MTT assay. Data normality was evaluated using the Shapiro-Wilk test, homogeneity using the Brown-Forsythe test, and differences between groups using two-way ANOVA, with statistical significance set at p <0.05. Results: Two-way ANOVA revealed that UV irradiation had no significant effect on cell viability (F=2.388; p=0.093). Similarly, SLA treatment showed no significant effect (F= 1.679; p = 0.207), and no significant interaction was observed between SLA treatment and UV-C irradiation (F=0.041; p=0.988). The highest mean cell viability was observed in the non-SLA group exposed to UV-C irradiation for 10 minutes (88.53 ± 25.22), whereas the lowest mean cell viability was found in the SLA group without UV-C irradiation (45.55 ± 16.29). Conclusion: Neither UV-C irradiation nor SLA treatment significantly affected MC3T3-E1 osteoblast cell viability, and no significant interaction was observed between the two treatments.
Co-Authors Adisty Restu Poetri Ahmad Syaify Ahmad Syaify, Ahmad Aini Moeljono Al Sri Koes Soesilowati Andreas Tjandra Angga Febriharta Anton Kusumo Widagdo Banun Kusumawardhani Citra Lestari Dahlia Herawati Danastri, Arifia Anindita Febriharta, Angga Fitri Yuniawati Fitri Yuniawati Flandiana Yogianti Hendry Dwi Wijayanto Heni Susilowati Kusumaatmaja, Ahmad Mefi Priba Sari Noormaya Sari Nunuk Purwanti Nur Rahman Ahmad Seno Aji Nurbaiti, Mentari Salma Oktarina, Dyah Ayu Mira Osa Amila Hafiyyah Pati Tangsupati Rani Gamawati Sri Pramestri Lastianny, Sri Pramestri Sudibyo sudibyo Suryono Suryono Suryono Suryono Tantin Ermawati Tissa Rahadianti Tjandra, Andreas Tutut Prabantari Anindyajati Vincensia Maria Karina