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All Journal Jambi Medical Journal : Jurnal Kedokteran dan Kesehatan Majalah Kedokteran Bandung Althea Medical Journal Indonesian Journal of Urology Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Jurnal Kesehatan Andalas Medical Laboratory Technology Journal Journal of Medicine and Health Majalah Patologi Indonesia JK Unila (Jurnal Kedokteran Universitas Lampung) Jurnal Ilmu Faal Olahraga Indonesia JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Indonesian Journal of Cancer Ophthalmologica Indonesiana Indonesian Journal of Endocrinology Metabolism and Diabetes (InaJEMD) Journal of Medicine and Health
Hasrayati Agustina
Departemen Patologi Anatomik Fakultas Kedokteran Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Dentistry Economics, Econometrics & Finance Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Neuroscience Nursing Public Health

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KEMAMPUAN PEWARNAAN ZIEHL-NEELSEN DAN FITE FARACO UNTUK MENDETEKSI MYCOBACTERIUM SP. PADA SAMPEL JARINGAN : A LITERATURE REVIEW Dwi Putri, Riska; Aminah Usman, Hermin; Agustina, Hasrayati
Majalah Patologi Indonesia Vol. 34 No. 2 (2025): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

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Abstract

Histopathology is an essential method for disease diagnosis. It is crucial for clinicians to have an ideal diagnostic method that is simple, specific, and highly sensitive. The sensitivity and specificity of a test can be determined by comparing it with other tests. Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis. Mycobacterium tuberculosis is diagnosed using Ziehl-Neelsen staining, which dif erentiates acid-fast bacilli from non-acid-fast bacilli. The Fite Faraco staining technique is used to detect Mycobacterium sp in tissue specimens. All reviewed articles show that Ziehl-Neelsen staining has a sensitivity between 21% - 97.6%, specificity between 85.7% - 92%, NPV between 34.3% - 75%, PPV between 30.9% - 100% in detecting Mycobacterium sp in tissue samples. Fite Faraco staining shows a sensitivity between 50% - 74.6%, specificity between 84% - 100%, NPV between 33.6% - 56.7%, PPV 38.1% in detecting Mycobacterium sp in tissue samples.It is detected that Ziehl-Neelsen and Fite Faraco can be used to detect bacteria, Mycobacterium sp especially bacteria Mycobacterium tuberculosis and Mycobacterium leprae. However, Ziehl-Neelsen staining has better ability in terms of sensitivity, PPV, and NPV than Fite Faraco in detecting bacteria Mycobacterium sp, especially Mycobacterium tuberculosis. As Fite-Faraco staining is superior in terms of specificity. Other things that must be Considered in carrying out Ziehl-Neelsen and Fite Faraco staining are specific types of samples, making modifications such as modifying microwave heating on the Ziehl-Neelsen staining method and combining examination with H&E staining and multiplex PCR to increase the validity of the two staining methods.
Co-Authors Abdul H. Hassan Abdul H. Hassan, Abdul H. Abdul Hadi Hassan, Abdul Hadi Afiati Afiati Aminah Usman, Hermin Andika Prahasta Azhar, Yohana Bethy S Hernowo Bethy S Hernowo, Bethy S Bethy S. Hernowo Bethy S. Hernowo Bethy Suryawathy Hernowo Bethy Suryawati Hernowo Bethy Suryawati Hernowo Birgitta Maria Dewayani Dewayani, Birgitta Maria Dewi, Yussy A Dimyati Achmad Djajakusumah, Teguh M Dwi Putri, Riska Edward Jaya Hadi Elsa Gustianty Giovani, Octavianus Hadaral Hudanul Qolbi Hermin Aminah Usman Herry Yulianti Hidayat, Peri Hikmat Permana Inas Susanti Kiki Akhmad Rizki, Kiki Akhmad Kusumawati, Maya Lili Sumarni Maman Abdurahman Maretha Amrayni Maula Rifada Mifatuhrachman Mifatuhrachman Mifatuhrachman, Mifatuhrachman Muhammad Iman Pratama Putra Nanny Natalia Mulyani Soetedjo Naomi, Sonya M Niswan Helja Batubara Nita Novita Nova Sylviana Octavianus Giovani Odilia Dos Reis, Odilia Dos Raden Erwin Affandi Soeriadi Koesoemah Ris Kristiana Ris Kristiana, Ris Ritonga, Ervita Siska Dwiyantie Wahyuni Sitanggang, Rupita Sari Endangena Sri Maryanti Sri Mulyati Sri Mulyati Sri Suryanti Susi Heryati Teguh M Djajakusumah Teguh Marfen Djajakusumah, Teguh Marfen Tutik Nur Ayni Yasni Iryani Widiasih Yenni Wisudarma Yenni Wisudarma Yenni Wisudarma, Yenni Yohana Azhar Yohana Azhar Yuktiana Kharisma Zahra Nurusshofa