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UJI AKTIVITAS ANTIBAKTERI FUNGI ENDOFIT PADA DAUN SENGGANI (Melastoma malabathricum L.) ASAL LAMPARA MONCONGLOE TERHADAP BAKTERI PENYEBAB INFEKSI SALURAN PENCERNAAN Hamdani, Nina; Kosman, Rachmat; Rusli, Rusli
Makassar Pharmaceutical Science Journal (MPSJ) Vol 1 No 3 (2023)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v1i3.91

Abstract

Senggani leaf (Melastoma malabathricum L.) is a plant that has antibacterial activity. The purpose of this study was to determine the isolate of the endophytic fungus senggani leaves (Melastoma malabathricum L.) which has antibacterial activity against digestive tract bacteria using the TLC-Bioautography method. The results of this study showed two isolates of senggani leaf endophytic fungi (Melastoma malabathricum L.). IFDS 6 obtained spots with Rf value = 0.58 for Eschericia coli bacteria, Rf value 0.67 for Vibrio cholerae, Salmonella typhi and Shigella dysentriae bacteria. Meanwhile, IFDS 8 obtained spots with an Rf value 0.78 on the bacteria Eschericia coli, Vibrio cholerae, Salmonella typhi, and Shigella dysentriae
POTENSI AKTIVITAS ANTIBAKTERI FUNGI ENDOFIT DAUN PARANG ROMANG (Boehmeria virgata) (G.Forst.) Guill TERHADAP BAKTERI UJI MICROCOCCUS LUTEUS DAN BACILLUS SUBTILIS SECARA KLT-BIOAUTOGRAFI FITRIANI, SITTI ANNISA; Kosman, Rachmat; Rusli, Rusli
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 4 (2025)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v2i4.299

Abstract

Parang romang (Boehmeria virgata (G.Forst) Guill) is a plant used to treat various infectious diseases, one of which is the digestive tract. Antibacterials are compounds that can inhibit or kill bacteria that cause infection. Endophytic fungi produce bioactive compounds in the form of antioxidants, anticancer, antiviral, antifungal and so on. This research uses the TLC-Bioatography method. The research started with the isolation of endophytic fungi and antagonist tests, which obtained active isolates against the test bacteria Micrococcus luteus and Bacillus subtilis, namely IFBV-6. Then this isolate was fermented and continued with testing using the TLC-Bioautography method. The resulting fermentate was dissolved in chloroform:methanol (5:1) solvent. Test results from TLC-Bioautography of IFBV-6 fermentate showed Rf values ​​of 0.9, 0.81 and 0.58. In the case of Micrococcus luteus and bacillus subtilis bacteria, good Rf values ​​from TLC show that there is quite good separation, ranging from 0.2-0.8, indicating clear antibacterial activity. In this test, each fermentation code has the same Rf value and number of spots. The results of identifying the chemical components of endophytic fungal isolates from parang romang leaves of Boehmeria virgata (G.Forst) Guill) gave positive results using alkaloid and saponin spotting.