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All Journal Jurnal Teknologi Dan Industri Pangan Jurnal Veteriner Microbiology Indonesia Journal of the Medical Sciences (Berkala Ilmu Kedokteran) The Indonesian Biomedical Journal Jurnal Locus Penelitian dan Pengabdian eJournal Kedokteran Indonesia Journal of Clinical Microbiology and Infectious Diseases
Pratiwi Pujilestari Sudarmono
Departemen Mikrobiologi, Fakultas Kedokteran, Universitas Indonesia, Jakarta
Aerospace Engineering Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Electrical & Electronics Engineering Immunology & microbiology Industrial & Manufacturing Engineering Mathematics Medicine & Pharmacology Neuroscience Public Health Veterinary

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Identifikasi Parameter Resistensi Moxifloxacin pada Pasien Tuberkulosis Dewi, Intan Keumala; Tunru, Insan Sosiawan; Sudarmono, Pratiwi Pujilestari; Umniyati, Helwiah; Mukhtar, Diniwati
Jurnal Locus Penelitian dan Pengabdian Vol. 4 No. 10 (2025): JURNAL LOCUS: Penelitian dan Pengabdian
Publisher : Riviera Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58344/locus.v4i10.5011

Abstract

Tuberkulosis (TBC) tetap menjadi masalah kesehatan global yang serius dan semakin kompleks dengan munculnya resistensi obat, termasuk terhadap moxifloxacin—salah satu antibiotik golongan fluoroquinolone yang digunakan dalam pengobatan TBC resisten obat, seperti MDR-TB dan XDR-TB. Resistensi terhadap moxifloxacin menjadi tantangan besar karena dapat memperpanjang durasi terapi, meningkatkan angka kegagalan pengobatan, serta memperbesar risiko penularan. Penelitian ini bertujuan untuk menganalisis mekanisme resistensi moxifloxacin pada Mycobacterium tuberculosis dengan mengidentifikasi mutasi genetik yang berperan serta mengevaluasi dampaknya terhadap efektivitas terapi. Metode yang digunakan adalah deskriptif laboratorium dengan uji sensitivitas obat (Drug Susceptibility Testing/DST) dan analisis sekuensing gen gyrA dan gyrB dari isolat klinis M. tuberculosis. Hasil penelitian menunjukkan adanya korelasi signifikan antara mutasi pada gen gyrA, khususnya pada kodon 90 dan 94, dengan tingkat resistensi tinggi terhadap moxifloxacin. Sementara itu, mutasi pada gen gyrB ditemukan lebih jarang, namun tetap berkontribusi terhadap resistensi tingkat sedang. Temuan ini menegaskan pentingnya penerapan diagnostik molekuler sebagai pelengkap uji konvensional untuk meningkatkan akurasi dan kecepatan deteksi resistensi. Implikasi penelitian ini menekankan perlunya skrining genotipik rutin dalam program pengendalian TBC agar terapi dapat disesuaikan dengan profil resistensi pasien, serta mencegah penyebaran lebih lanjut dari strain resisten obat. Penguatan surveilans dan deteksi dini resistensi fluoroquinolone menjadi langkah strategis untuk mendukung keberhasilan pengendalian TBC secara global.
Identification test of bacterial species and resistance genes from infectious patients using MDR direct flow chip molecular technique compared to conventional techniques Surjawinata, Mario; Sudarmono, Pratiwi; Lestari, Resti Hardianti
Journal of Clinical Microbiology and Infectious Diseases Vol. 5 No. 2 (2025): Available online : 1 December 2025
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v5i2.89

Abstract

Introduction: Antimicrobial resistance poses a threat to global public health by increasing morbidity and mortality. While resistance development is a natural biological process, antibiotic use has accelerated its rise resistance. This study aimed to determine the sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of the MDR Direct Flow Chip Kit to detect 5 bacterial species and a total of 55 resistance gene markers. Methods: The research employed a cross-sectional design, analyzing positive samples determined by the VITEK® 2 system from various specimens, including sputum, pus, and blood. These specimens were collected from sepsis patients at the FKUI Clinical Microbiology Laboratory in 2023. They were stored in Eppendorf tubes with TSB and Glycerol fluids, then cultured on Nutrient Agar Media and incubated overnight. HybriSpot® will analyze the increasing isolates using the MDR Direct Flow Chip, an in vitro diagnostic kit that quickly detects multidrug-resistant bacteria. This kit employs multiplex PCR to identify five bacterial species—Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli, and Acinetobacter baumannii—as well as 55 resistance gene markers. These markers include the primary enzymatic mechanisms associated with nine different antibiotic classes. Result: A total of 40 samples were analyzed with HybriSpot® using the MDR Direct Flow Chip, which identified 5 genus species and detected 31 resistance genes. The same samples were also tested with the VITEK® 2 system, which identified the same 5 genus species and provided antibiotic susceptibility results. The overall sensitivity was 80%, specificity was 94.2%, and conformity was 94.68%. Conclusion: The MDR Direct Flow Chip assay is a promising method for identifying pathogens in positive blood cultures of infected patients and can aid antimicrobial stewardship programs in hospitals.
Co-Authors . Andriansjah Ade Dharmawan Agus Sjahrurachman AHMAD WIBISANA Amin Soebandrio Andi Yasmon Anis Karuniawati Ariyani Kiranasari Asmarani Kusumawati Augustine Natasha Augustine Natasha Azmier Adib Betty Ernawati Chairunissa T. Rizal Cleopas Martin Rumende Delly Chipta Lestari Dewi, Intan Keumala Diniwati Mukhtar Fera Ibrahim Fera lbrahim Fithriyah Fithriyah Fithriyah Fithriyah HAK HOTTA Helwiah Umniyati Hidetaka Tsuji Holy Arief Ingrid S. Surono Leonardus B.S. Kardono Lestari, Resti Hardianti Lola Febriana Dewi Louisa Ivana Utami Mardiastuti H Wahid Mardiastuti Mardiastuti Mirawati Sudiro Myrna Adianti Nie Nie Prawoto Prawoto Rela Febriani Robert Utji Shirly Kumala Surjawinata, Mario Susan Maphilindawati Noor Sweety Pribadi Tjahjani M. Sudiro TJAHJANI MIRAWATI SUDIRO Tomohiro Oshibe Triyani Sukarso Tunru, Insan Sosiawan VERONICA WIWING Vivi Lisdawati Wahono Sumaryono Yeva Rosana Yulia R. Saharman Yulia Rosa Saharman