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All Journal Jurnal Teknologi Dan Industri Pangan Microbiology Indonesia Journal of the Medical Sciences (Berkala Ilmu Kedokteran) The Indonesian Biomedical Journal Jurnal Locus Penelitian dan Pengabdian eJournal Kedokteran Indonesia Journal of Clinical Microbiology and Infectious Diseases Journal of Innovative and Creativity
Pratiwi Pujilestari Sudarmono
Departemen Mikrobiologi, Fakultas Kedokteran, Universitas Indonesia, Jakarta
Aerospace Engineering Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Education Electrical & Electronics Engineering Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Neuroscience Public Health Social Sciences Other

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Identification test of bacterial species and resistance genes from infectious patients using MDR direct flow chip molecular technique compared to conventional techniques Surjawinata, Mario; Sudarmono, Pratiwi; Lestari, Resti Hardianti
Journal of Clinical Microbiology and Infectious Diseases Vol. 5 No. 2 (2025): Available online : 1 December 2025
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v5i2.89

Abstract

Introduction: Antimicrobial resistance poses a threat to global public health by increasing morbidity and mortality. While resistance development is a natural biological process, antibiotic use has accelerated its rise resistance. This study aimed to determine the sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of the MDR Direct Flow Chip Kit to detect 5 bacterial species and a total of 55 resistance gene markers. Methods: The research employed a cross-sectional design, analyzing positive samples determined by the VITEK® 2 system from various specimens, including sputum, pus, and blood. These specimens were collected from sepsis patients at the FKUI Clinical Microbiology Laboratory in 2023. They were stored in Eppendorf tubes with TSB and Glycerol fluids, then cultured on Nutrient Agar Media and incubated overnight. HybriSpot® will analyze the increasing isolates using the MDR Direct Flow Chip, an in vitro diagnostic kit that quickly detects multidrug-resistant bacteria. This kit employs multiplex PCR to identify five bacterial species—Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli, and Acinetobacter baumannii—as well as 55 resistance gene markers. These markers include the primary enzymatic mechanisms associated with nine different antibiotic classes. Result: A total of 40 samples were analyzed with HybriSpot® using the MDR Direct Flow Chip, which identified 5 genus species and detected 31 resistance genes. The same samples were also tested with the VITEK® 2 system, which identified the same 5 genus species and provided antibiotic susceptibility results. The overall sensitivity was 80%, specificity was 94.2%, and conformity was 94.68%. Conclusion: The MDR Direct Flow Chip assay is a promising method for identifying pathogens in positive blood cultures of infected patients and can aid antimicrobial stewardship programs in hospitals.
Potensi Nigella sativa sebagai Agen Antiinflamasi dan Antioksidan dalam Terapi Acne Vulgaris: Tinjauan Literatur Taufiq, Angelica Vanini Winata; Sudarmono, Pratiwi Pujilestari; Widiyanti, Dian; Harliansyah, Harliansyah
Journal of Innovative and Creativity Vol. 6 No. 2 (2026)
Publisher : Fakultas Ilmu Pendidikan Universitas Pahlawan Tuanku Tambusai

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Abstract

Acne vulgaris merupakan penyakit inflamasi kronis pada unit pilosebasea yang melibatkan hiperkeratinisasi folikel, produksi sebum berlebih, kolonisasi Cutibacterium acnes, inflamasi, dan stres oksidatif. Terapi konvensional acne vulgaris sering menimbulkan efek samping, iritasi, serta resistensi antibiotik, sehingga diperlukan alternatif terapi yang lebih aman dan efektif. Nigella sativa dikenal sebagai tanaman herbal yang memiliki kandungan bioaktif utama berupa thymoquinone dengan aktivitas antiinflamasi, antioksidan, dan antimikroba. Review ini bertujuan mengkaji potensi Nigella sativa sebagai agen antiinflamasi dan antioksidan dalam terapi acne vulgaris. Metode yang digunakan adalah narrative review melalui penelusuran literatur pada basis data PubMed, Scopus, dan Google Scholar dengan rentang publikasi tahun 2014–2025 menggunakan kata kunci Nigella sativa, thymoquinone, acne vulgaris, inflammation, dan oxidative stress. Hasil kajian menunjukkan bahwa thymoquinone mampu menghambat jalur nuclear factor kappa B (NF-κB), menurunkan ekspresi IL-1β, IL-6, dan TNF-α, serta menekan proses inflamasi pada lesi acne. Selain itu, Nigella sativa meningkatkan aktivitas antioksidan endogen seperti superoxide dismutase (SOD), glutathione (GSH), dan katalase, sehingga dapat mengurangi kerusakan jaringan akibat reactive oxygen species (ROS). Aktivitas antimikroba terhadap bakteri penyebab acne juga mendukung efektivitasnya sebagai terapi topikal. Berbagai formulasi seperti gel, hidrogel, dan emulgel menunjukkan hasil klinis yang baik dan aman. Temuan ini menegaskan bahwa Nigella sativa berpotensi sebagai terapi tambahan atau alternatif yang efektif dan aman pada acne vulgaris.
Co-Authors . Andriansjah Ade Dharmawan Agus Sjahrurachman AHMAD WIBISANA Amin Soebandrio Andi Yasmon Anis Karuniawati Ariyani Kiranasari Asmarani Kusumawati Augustine Natasha Augustine Natasha Azmier Adib Betty Ernawati Chairunissa T. Rizal Cleopas Martin Rumende Delly Chipta Lestari Dewi, Intan Keumala Dian Widiyanti Diniwati Mukhtar Fera Ibrahim Fera lbrahim Fithriyah Fithriyah Fithriyah Fithriyah HAK HOTTA Harliansyah, Harliansyah Helwiah Umniyati Hidetaka Tsuji Holy Arief Ingrid S. Surono Leonardus B.S. Kardono Lestari, Resti Hardianti Lola Febriana Dewi Louisa Ivana Utami Mardiastuti H Wahid Mardiastuti Mardiastuti Mirawati Sudiro Myrna Adianti Nie Nie Prawoto Prawoto Rela Febriani Robert Utji Shirly Kumala Surjawinata, Mario Susan Maphilindawati Noor Sweety Pribadi Taufiq, Angelica Vanini Winata Tjahjani M. Sudiro TJAHJANI MIRAWATI SUDIRO Tomohiro Oshibe Triyani Sukarso Tunru, Insan Sosiawan VERONICA WIWING Vivi Lisdawati Wahono Sumaryono Yeva Rosana Yulia R. Saharman Yulia Rosa Saharman