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All Journal PROSIDING SEMINAR KIMIA Jurnal Kimia Mulawarman Science and Technology Indonesia JURNAL ATOMIK PRIMER: Jurnal Ilmiah Multidisiplin al Kimiya : Jurnal Ilmu Kimia dan Terapan Journal of Science and Technology: Alpha
Djihan Ryn Pratiwi, Djihan Ryn
Jurusan Kimia FMIPA Universitas Mulawarman
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Energy Environmental Science Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Physics Social Sciences

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THE PHYTOCHEMICAL TEST AND ANTIOXIDANT ACTIVITY (DPPH METHOD) FROM LEAVES OF BIRIBA (Rollinia mucosa (Jacq.) Baill.) Sumarni, Feni; Saleh, Chairul; Pratiwi, Djihan Ryn
JURNAL ATOMIK Vol 4 No 1 (2019)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Phytochemical test and antioxidant activity test of biriba leaves (Rollinia mucosa (Jacq.) Baill.) has been done. Extraction from biriba leaves was carried out using several solvents, namely ethanol, n-hexane and ethyl acetate. From phytochemical test results from leaves of biriba (R. mucosa) containing secondary metabolites namely alkaloids, phenolics, flavonoids and steroids. Based on the results of the antioxidant test using DPPH (1,1-diphenyl-2-picrylhidrazyl) method showed that the crude extract, n-hexane fraction, ethyl acetate fraction and residual ethanol fraction had IC50 values of 159.72; 119.62; 40.12 and 38.35 ppm, respectively. From the results of the antioxidant test it is known that the ethanol fraction of the remaining biriba leaves has the strongest antioxidant activity with IC50 values of 38.35 ppm.
UJI FITOKIMIA DAN AKTIVITAS ANTIBAKTERI EKSTRAK METANOL AKAR MERUNG (Coptosapelta tomentosa) Dewi, Dynda Rosiana; Marliana, Eva; Pratiwi, Djihan Ryn
JURNAL ATOMIK Vol 4 No 2 (2019)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Phytochemical tests and antibacterial activity of the roots of Merung plants (Coptosapelta tomentosa) have been done. This research aims to find out the content of secondary metabolites contained in the sample using the Thin Layer Chromatography method and to determine antibacterial activity by paper disc method from methanol extract Merung root (Coptosapelta tomemtosa). Based on results of phytochemical screening, it was known that methanol extract contained alkaloid, polyphenol, flavonoid and terpenoid/steroid. Methanol extract antibacterial activity against Propionibacterium acnes KCCM 11898 range of MIC of 0-1%. Methanol extract showed antibacterial activity against Escherichia coli bacteria ATCC 25922 range of MIC of 0-1%. From the results of the antibacterial activity test it can be concluded that the methanol extract have antibacterial activity against Propionibacterium acne KCCM 11898 and Escherichia coli ATCC 25922.
AKTIVITAS AMILASE PENDEGRADASI PATI MENTAH DARI BAKTERI AIR SUNGAI KARANG MUMUS Adawiyah, Rabiatul; Astuti, Winni; Pratiwi, Djihan Ryn
JURNAL ATOMIK Vol 10 No 1 (2025)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/ja.v10i1.1409

Abstract

Raw starch degrading amylase (RSDA) is an enzyme that can degrade raw starch into glucose without going through the gelatinization process. This research was conducted to isolate RSDA producing bacteria originating from the Karang Mumus River in Samarinda City. Bacterial isolation was carried out using the spread plate method. Screening for RSDA producing bacteria using nutrient media containing raw starch. The results of bacterial isolation obtained 14 single colonies of bacteria. A total of 5 bacterial colonies out of 14 bacteria were able to produce RSDA. Then the RSDA enzyme from the 5 bacteria was tested for amylase activity quantitatively using the dinitrosalicylic acid (DNS) method. Then, of the 5 bacteria, 1 bacterium with the colony code KM 5 had the highest activity, namely 0,332 U/mL.
ISOLASI DAN KARAKTERISASI LIPASE DARI KECAMBAH BIJI ALPUKAT (Persea americana Mill) Sya'bani, Nurillah; Astuti, Winni; Pratiwi, Djihan Ryn
JURNAL ATOMIK Vol 2 No 2 (2017)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Isolation and determination of optimum lipase conditions from Avocado seeds germination (Persea americana Mill) have been done. The purpose of this study is to determine the optimum working conditions and lipase specific activity of avocado seeds germination. Lipase was isolated using homogenization and centrifugation techniques. The crude extract of the enzyme containing the lipase produced from avocado seeds germination was used for the determination of total protein concentration and tested its activity on the pH conditions, temperature, and optimum substrate concentration with different with various variations. Determination of total concentration was done by Bradford method, while the determination of optimum working conditions and lipase specific activity was performed by titrimetric method. The results showed that total protein concentration in avocado seeds germination was 458 μg / mL. The optimum working conditions on avocado seed sprouts are the degree of acidity (pH) 6, temperature 35 oC, and 1.5% v/v substrate concentration. The lipase specific activity of avocado seeds germination is 144,1 U / mg.
The Utilization of Chitosan from Maggot (Hermetia illucens) Exuvia as Edible Coating for Tomatoes (Lycopersicon esculentum) and Edible Film with the Addition of Honey as an Antibacterial Agent Daniel; Sitorus, Saibun; Pasaribu, Subur P.; Marliana, Eva; Hairani, Rita; Ruga, Ritbey; Purba, Ritson; Saleh, Chairul; Pratiwi, Djihan Ryn; Magdaleni, Agustina Rahayu; Masmur, Indra; Masmur, Hestina
Science and Technology Indonesia Vol. 10 No. 4 (2025): October
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.4.1232-1241

Abstract

The use of chitosan from maggot (Hermetia illucens) exuvia as an edible coating for tomatoes (Lycopersicon esculentum) and an edible film with honey added as an antibacterial agent was investigated. This study aimed to determine the yield of chitosan from black soldier fly (BSF, Hermetia illucens) maggot exuviae as an edible coating. The chitosan was produced from chitin by deacetylation, and its effect on the weight loss and vitamin C levels of tomatoes for 7 days after treatment was investigated. The chitosan coating was characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy. The antibacterial activity was evaluated using a disk diffusion method. The result showed that by deacetylation, conversion of chitin produced chitosan with a yield of 72% and a deacetylation degree of 75.05%. The chitosan coating significantly affected the weight loss of tomatoes, with the best concentration being 2%. However, the treatment did not significantly affect the decrease in vitamin C levels. The edible chitosan film from BSF maggot exuviae with added honey had a thickness, water content, and water vapor transmission rate of 0.156 mm, 16.9913%, and 30.45 g/m2/24 hours, respectively. SEM characterization showed a relatively dense surface structure, which was slightly smooth and porous. Regarding antibacterial activity, the edible chitosan film inhibited Staphylococcus aureus with an inhibition zone of 10.37 mm; however, Escherichia coli was not inhibited. These results suggest that chitosan from BSF maggot exuviae has potential as an effective edible coating for reducing weight loss in tomatoes despite showing limited antibacterial properties.
Penurunan Konsentrasi Besi dan Mangan Terlarut pada Air Tanah Muara Lawa Menggunakan Serbuk Kiambang (Salvinia molesta) Widodo, Nanang Tri; Sari, Ika Yekti Liana; Pratiwi, Djihan Ryn
Journal of Science and Technology: Alpha Vol. 1 No. 2 (2025): Journal of Science and Technology: Alpha, April 2025
Publisher : Lembaga Publikasi Ilmiah Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70716/alpha.v1i2.161

Abstract

One of the primary sources of water for human needs is groundwater. Groundwater serves various household purposes, such as bathing, cooking, drinking, and washing. The presence of iron and manganese in groundwater has long been a serious concern, as these elements can impart an unpleasant taste and odor to the water. This study aims to determine the reduction levels of dissolved iron and manganese concentrations in groundwater using powdered water fern (Salvinia molesta) as an adsorbent. The reduction of iron and manganese concentrations was carried out by adsorbing these elements from groundwater using activated powdered water fern. The results showed that the concentrations of iron and manganese in the groundwater were reduced by 64.77% and 77.67%, respectively.
SELEKSI AMILASE PENDEGRADASI PATI MENTAH BAKTERI DARI AIR GALIAN BEKAS TAMBANG BATU BARA DI DESA LOA BAHU Akbar, Akbar; Astuti, Winni; Pratiwi, Djihan Ryn
JURNAL ATOMIK Vol 10 No 2 (2025)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/ja.v10i2.1546

Abstract

Raw starch-degrading amylase (RSDA) is a type of hydrolase enzyme that can degrade raw starch into glucose without going through the heating and gelatinization process. This study was conducted to select RSDA-producing bacteria from ex-coal mine water samples in Loa Bahu Village, Sungai Kunjang, Samarinda, with various types of raw starch such as rice, wheat, corn, potatoes, sago, and cassava. The selection of bacteria-producing RSDA enzymes was carried out using agar nutrient media containing raw starch, the positive test was marked by the formation of a clear zone around the bacterial colony after the addition of iodine solution. A total of 13 bacterial isolates (AT 1 - AT 13) were tested qualitatively by RSDA. The selection results showed that 4 bacterial isolates were capable of degrading raw rice starch, 4 bacterial isolates were capable of degrading raw corn starch, 2 bacterial isolate was capable of degrading raw cassava starch, 1 bacterial isolate was capable of degrading raw potato starch, 1 isolate was capable of degrading raw wheat starch, and 2 bacterial isolates were able to degrade raw sago starch. The bacterial isolate with the code AT3 is capable of degrading raw starch of rice, corn, potatoes, wheat and sago.
Co-Authors Ade Dinaran Akbar Akbar Akkas, Erwin Allo, Veliyana Londong Ami, Suyatmi Angellina Putri Petrisia Anita Karolina, Anita Arifwan - Aris Munandar Aristina, Reza Fauziah Cahyani, Amelya Putri Chairul Saleh Chairul Saleh Daniel Dewi, Dynda Rosiana Erwin Erwin Erwin Erwin Eva Marliana, Eva Haeruddin Haeruddin Hairani, Rita Hermawan Hermawan Ilham, Muhammad Hafid Indah Ashari Rahmadani Indra Masmur Lianasari, Ika Yekti Magdaleni, Agustina Rahayu Masmur, Hestina Maulina, Sheli Nanang Tri Widodo Nur Hidayah Putri, Amanda Aulia Rabiatul Adawiyah Ritson Purba, Ritson Rudi Kartika, Rudi Ruga, Ritbey Saibun Sitorus Sari, Ika Yekti Liana Soerja Koesnarpadi Subur P Pasaribu Sumarni, Feni Sya'bani, Nurillah Teguh Wirawan, Teguh Tri Riski Amalia Usdin, Muhammad Sadam Vera, Hendrica Mini Wahyu Budi Utama Wahyu Teguh Santoso Winni Astuti Winni Astuti, Winni