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All Journal Journal SAGO Gizi dan Kesehatan Jurnal Natural Jurnal Medika Veterinaria
Frengki Frengki, Frengki
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Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Earth & Planetary Sciences Energy Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Veterinary

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Multi-drug resistance-1 (MDR-1) gene expression in MCF-7 cells after treated with doxorubicin-deoxyelephantopin combination and prediction of inhibitory activity against Pgp receptors with in silico DANIEL, DANIEL; FRENGKI, FRENGKI; JALALUDDIN, MUHAMMAD; SARI, WAHYU EKA; ROSMAIDAR, ROSMAIDAR; HASRIATI, HASRIATI; ZAKIAH, NONI
Jurnal Natural Volume 24 Number 3, October 2024
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v24i3.33237

Abstract

Doxorubicin chemotherapy has been a strong focus in breast cancer research. Side effects, toxicity and resistance have been extensively studied. One proposed solution to these issues that its combination with deoxyelephantopin. Deoxyelephantopin is known to be toxic in many cancer cells but safe in normal cells. IC50 of each compound were determined by using a MTT assay, and the MDR-1 gene mRNA expression were determined by using qPCR method, while the interaction of doxorubicin in combination with deoxyelephantonin on Pgp receptor were predicted by using an in silico approach. The IC50 of deoxyelephantopin was found to be 11.2 g/mL, while IC50 of doxorubicin was 448 nM IC50 values showed a deoxyelephantopin-doxorubicin combination was able to reduce MDR-1 expression by 19% compared to doxorubicin and IC50 values indicated that the combination formula reduced the expression by 15% over doxorubicin alone. The affinity of doxorubicin and deoxyelephantopin is -12.16 kcal/mol and -9.51 kcal/mol, respectively, while the affinity of doxorubicin after combine with deoxyelephantopin decreases from -12,16 kcal/mol to -11,25 kcal/mol due to the release of one Leu 221 hydrogen bond. The combination of doxorubicin with deoxyelephantopin is able to reduce expression and suppress the function of the Pgp protein.
Screening of Bioactive Compounds of Gambir leaf (Uncaria gambir Roxb) as Antibiofilm Againts the Glucosyltransferase of Streptococus mutans Enzyme Khalid, Idham; Sari, Dea Kurnia; Bakri, Muttaqien; Daniel, Daniel; Frengki, Frengki; Makmur, Ali; Riandi, Lian Varis; Ferdian, Riyan; Amiruddin, Amiruddin
Jurnal Medika Veterinaria Vol 18, No 2 (2024): J. Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v18i2.43265

Abstract

The use of natural ingredients in dental care, such as manginang which has become part of the Minang community culture, is considered an alternative dental care that must be created to overcome the negative impacts of dental care made from artificial materials. The core of this study is to change the manginang tradition towards logical verification through screening of bioactive compounds of gambir leaves (Uncaria gambir Roxb.) as antibiofilms against Streptococcus mutans. This study was conducted using an in silico strategy approach with the help of computer programs such as MOE v.09 and Chimera 1.13.1, as well as the Way2Drug site, protein information bank, and protox_ii. This study began with the collection of ligand information from the writing, followed by transformation into 2D and 3D designs. Other antibiofilm actions were decided using the QSAR procedure through the website (http://www.way2drug.com/PASSOnline/predict.php). The selected antibiofilm compounds were then followed by observing their affinity for the receptor (pdb id. 3AIB) using an atomic docking strategy. Finally, the ligand damage profile was observed to anticipate. The results of this study indicate that of the 41 identified gambir leaf compounds, there are 20 compounds that have the potential to have antibiofilm effects with quinnic acid having the highest score (Pa 0.703), while the compound with the strongest affinity for the receptor is chlorogenic acid with an affinity score (Gbinding) of -16.03 kcal/mol and an average of low toxicity (level IV-VI). However, of the 20 bioactive compounds of gambir leaves, only 5 compounds are predicted to have the same potential as the -maltose control.
Variasi tipe pelarut dan pengaruhnya terhadap kadar flavonoid total daun kirinyuh (Chromolaena odorata) yang tumbuh di kawasan geotermal Ie Seum Aceh Besar menggunakan spektrofotometri uv-vis Munira, Munira; Zakiah, Noni; Handayani, Rini; Nasir, Muhammad; Frengki, Frengki
Jurnal SAGO Gizi dan Kesehatan Vol 6, No 2 (2025): Agustus
Publisher : Poltekkes Kemenkes Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30867/gikes.v6i2.2575

Abstract

Background: One plant that can be a source of natural healing for various ailments is the kirinyuh (Chromolaena odorata). This plant can grow in geothermal areas. Plants grown in geothermal areas have the potential to produce higher levels of active substances. The kirinyuh plant is rich in flavonoid compounds. Flavonoids have antioxidant, anti-inflammatory, antibacterial, antiviral properties, and have potential as anticancer agents. The level and quality of flavonoid compounds are influenced by the type of solvent.Objectives: This study was conducted to determine the total flavonoid content of kirinyuh leaf extract from the Ie Seum geothermal area of Aceh Besar based on different types of solvents.Methods: Determination of total flavonoid content using UV-Vis spectrophotometry and quercetin was used as a standard in the measurement. Each measurement was repeated three times. The results were analyzed using an independent t-test.Results: The results of the determination of total flavonoid levels obtained in kirinyuh leaves using ethanol solvent were 10,65 mg QE/g and ethyl acetate was 8,97 mg QE/g. Based on the results of the Independent T-test, a significance result of 0,000 (P<0,05) was obtained. These results indicate that there is a difference in total flavonoid levels between ethanol and ethyl acetate solvents.Conclusion: The total flavonoid content using ethanol solvent types were higher than ethyl acetate.
Co-Authors Amiruddin Amiruddin Daniel Daniel Ferdian, Riyan Hasriati Hasriati, Hasriati Idham Khalid Makmur, Ali Muhammad Jalaluddin, Muhammad Muhammad Nasir Munira Munira, Munira Muttaqien Bakri, Muttaqien Noni Zakiah Riandi, Lian Varis Rini Handayani Rosmaidar Rosmaidar, Rosmaidar Sari, Dea Kurnia Sari, Wahyu Eka