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All Journal Sainteknol : Jurnal Sains dan Teknologi Journal of Biology Education JCES (Journal of Character Education Society) Journal of Dedicators Community Life Science Jurnal Pengabdian Kolaborasi dan Inovasi IPTEKS Prosiding Seminar Nasional Biologi Journal of Biology Education Journal of Community Empowerment
Ely Rudyatmi, Ely
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Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Public Health Social Sciences

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Journal : Life Science

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AKTIVITAS ANTIOKSIDAN DAN KADAR KLOROFIL KULTIVAR SINGKONG DI DAERAH WONOSOBO Solikhah, Restanti; Purwantoyo, Eling; Rudyatmi, Ely
Life Science Vol 8 No 1 (2019): April 2019
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v8i1.30001

Abstract

Cassava leaves are the main barrier for oxidation reactions. This happens because of the chlorophyll content, but chlorophyll levels will increase with age until the leaves develop fully and then chlorophyll levels decrease when the leaves get older. When the leaves are old it is indicated that there are other compounds that act as the main barrier for oxidation reactions, namely flavonoids. Flavonoid is seen from the antioxidant activity of the leaves. The purpose of this study was to determine the levels of chlorophyll, antioxidant activity and the relationship between chlorophyll levels and antioxidant activity. This study uses seven cassava cultivars in Wonosobo. The leaves were extracted using the maceration method then the antioxidant activity was measured using DPPH method besides chlorophyll content was measured using a spectrophotometer using the ARNON method, then from the results of measurements of chlorophyll content and antioxidant activity in the Pearson Correlation method correlation test. Cassava results from Wonosobo cultivars Marsinah had the highest antioxidant activity of 71.13%. Furthermore Martapura (46.27%), Green Handle Vegetables (46.27%), Palengka (46.13%), Rubber (39.64%), Red Handle Vegetables (39.36%) and the lowest one is Kastepe cultivars that is 36.87%. While the highest chlorophyll content of Marsinah cultivars is 32.19 mg / l. Next Rubber (29.44 mg / l), Green Vegetable Handle (28.04 mg / l), Kastepe (27.66 mg / l), Martapura (27.30 mg / l), Palengka (22.82 mg / ll) and the lowest is the Red Handle Vegetable cultivar which is 22.01 mg / l. From these results, it can be concluded that ducks There is no correlation between chlorophyll levels and antioxidant activity of cassava cultivars in Wonosobo district. Daun singkong merupakan barrier utama untuk reaksi oksidasi. Hal ini terjadi karena adanya kandungan klorofil, akan tetapi kadar klorofil akan meningkat seiring bertambahnya umur sampai daun berkembang penuh dan kemudian kadar klorofil menurun ketika daun semakin tua. Pada saat daun sudah tua diindikasikan bahwa ada senyawa lain yang berperan sebagai barrier utama untuk reaksi oksidasi yaitu flavonoid. Falvonoid dilihat dari aktivitas antioksidan daun. Tujuan penelitian ini adalah mengetahui kadar aklorofil, aktivitas antioksidan dan hubungan anatara kadar klorofil dengan aktivitas antioksidan. Penelitian ini menggunakan tujuh kultivar singkong yang ada di wonosobo. Daun diekstraksi menggunakan metode maserasi kemudian diukur aktivitas antioksidannya menggunakan metode DPPH selain itu kadar klorofil diukur menggunakan spektrofotometer menggunakan metode ARNON, kemudian dari hasil pengukuran kadar klorofil dan aktivitas antioksidan di uji korelasi metode Pearson Correlation. Hasil menunjukkan Singkong dari Wonosobo kultivar Marsinah memiliki aktivitas antioksidan yang paling tinggi yaitu 71,13%. Selanjutnya Martapura (46,27%), Sayur Gagang Hijau (46,27%), Palengka (46,13%), Karet (39,64%), Sayur Gagang Merah (39,36%) dan yang terendah adalah kultivar Kastepe yaitu 36,87%. Sedangkan kadar klorofil yang paling tinggi kultivar Marsinah yaitu 32,19 mg/l. Selanjutnya Karet (29,44 mg/l), Sayur Gagang Hijau (28,04 mg/l), Kastepe (27,66 mg/l), Martapura (27,30 mg/l), Palengka (22,82 mg/l) dan yang terendah adalah kultivar Sayur Gagang Merah yaitu 22,01 mg/l. Dari hasil tersebut dapat disimpulkan bahwa tidak terdapat hubungan antara kadar klorofil dengan aktivitas antioksidan kultivar singkong di kabupaten wonosobo Keywords: Aktivitas Antioksidan, Kadar Klorofil, Kultivar Singkong
Karakter Anatomi dan Kandungan Pigmen Daun Mangga Wirasangka (Mangifera indica var. Wirasangka) Rahayu, Reny; Rahayu, Enni Suwarsi; Rudyatmi, Ely; Widiatningrum, Talitha
Life Science Vol 13 No 1 (2024): May 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v13i1.64386

Abstract

Wirasangka mango is the identity flora of Tegal Regency. The morphological characters of the wirasangka mango have been studied, but need to be supplemented with anatomical characters. The anatomical character of the plant that needs to be studied is the anatomy of the leaves. Leaves have varying tissue structures and pigment content. This study aims to describe the anatomical characters and analyze the pigment content of wirasangka mango leaves. The characteristics and pigment content of wirasangka mango leaves have never been reported. Anatomical characters of mango wirasangka leaves were determined by cross-sectional leaf preparations using non-embedding method and whole mount leaf preparations. The pigment content of mango wirasangka leaves was analyzed using a spectrophotometer. The results of the study of the anatomical characters showed that the leaves of wirasangka mango have dorsiventral and hypostomatous leaf, have a palisade ratio of 4-5 cells, stomata are anomocytic type, guard cell length is 11.45 µm and guard cell width is 4.78 µm, and has a stomatal density of 173.25 /mm2. The chlorophyll content of shoots, young and mature leaves of wirasangka mango were 9.1 mg/l, 12.28 mg/l and 47.89 mg/l. The carotenoid content of shoots, young leaves and mature leaves were 293.51 µmol/g, 371.76 µmol/g, and 1445.88 µmol/g respectively, and the anthocyanin content of shoots, young leaves and mature leaves were respectively also 1.36 mg/100g, 0.75 mg/100g, and 0.38 mg/100g. The results of the research can be used to complete the characters that need to be observed in the characterization of wirasangka mango.
Co-Authors Aditya Marianti Alaena Naelu Muthmainnah, Alaena Naelu Andhika, Pedro Ari Yuniastuti Asyifa, Bella Firdha Asyifa, Bella Firdha Avriana, Lilih B., F. Putut Martin H. Caddafie, Syahrizal Umar Dewa, Pramaditya Putra Komala Dewi Liesnoor Setyowati Dewi Mustikaningtyas Durrotun Nafisah Dyah Rini Indriyanti Eling Purwantoyo Endah Peniati Enni Suwarsi Rahayu Handayani, Rima IKA DAMAYANTI Ita Martini, Ita Krispinus Kedati Pukan Kusuma, Zeni Herawati Laksono Trisnantoro Lina Herlina Lisdiana Mahfiroh, Miskiyati Saroya Mighfar Khamida, Mighfar Muhammad Abdullah Mu’afy, Faizal Daffa Nana Kariada Trimartuti Ning Setiati Ning Setiati Nugrahaningsih WH, Nugrahaningsih Nur Liya Khasanah, Nur Liya Nur Rahayu Utami Nurjanah, Ayu Ika Nurjanah, Ayu Ika Puji Hardati Rahayu, Reny Rahmadani, Riska Arifa Rohman, Shohihatur Rosaningdyah, Amalia Rizqi Saiful Ridlo Sari, Nur Hidayati Puspita Setiyanto, Denny Firman Sindy Nurinda, Sindy Siti Harnina Bintari Solikhah, Restanti Sovia Mega Lestari Sri Mulyani Endang Susilowati, Sri Mulyani Endang Sri Sukaesih Sri Sukaesih Sriyadi , Sriyadi Sugianto Sukmawati Sukmawati Suryani Suryani Talitha Widiatningrum Thriwaty Arsal Tri Astuti, Diyah Ayu Tyas Agung Pribadi, Tyas Agung Ufi Saraswati, Ufi Ulfa, Berlina Maika Umi Rohayati, Umi Utami, Hanna Dewi Utami, Hanna Dewi Vera Widyaningsih, Vera Wiwi Isnaeni Wiwi Isnaini Wulan Christijanti Yasin, Ahmad Alfian Risydan Yasin, Ahmad Alfian Risydan Yeri Sutopo