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Heni Wijayanti, Heni
SMKN 3 Pati
Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : Greensphere: Journal of Environmental Chemistry

 1 
EFEKTIVITAS KRIM FORMULASI TEH HIJAU (Camellia sinensis) SEBAGAI AGEN ANTIBAKTERI TERHADAP PATOGEN KULIT Prayogo, Fitra Adi; Wijayanti, Heni; Rahmawati, Novita; Budiharjo, Anto; Wulandari, Dyah
Greensphere: Journal of Environmental Chemistry Vol 5, No 1 (2025): Volume 5 Nomor 1 Tahun 2025
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/gjec.2025.26815

Abstract

This research aims to determine the effect of green tea extract cream 6%, 8%, and 10% w/w against Staphylococcus epidermidis and Propionibacterium acne. The method begins with making green tea extract using the maceration method, and qualitative flavonoid, alkaloid, saponin, and tannin tests are carried out. Next, S. epidermidis and P. acnes were rejuvenated on NA media and cultured at 37°C for 24 hours. Both bacteria were then inoculated into different test tubes containing 0.9% NaCl. The suspension was then vortexed, and the turbidity level was equalized with McFarland's solution. Both bacteria were then cultured on NA media, and paper disks that had previously been soaked in clindamycin (control +) and 0%, 6%, 8%, and 10% green tea extract cream were placed on the agar media. The culture was then incubated for 24 hours at 37°C, and the inhibition zone was checked with a caliper. Results show that green tea extract has positive tests for flavonoids, alkaloids, saponins, and tannins. The optimum concentration of extract cream on antibacterial activity against S. epidermidis and P. acne is 10%. Inhibition zone comparison showed a significant difference between the diameter of the inhibition zone of S. epidermidis and P. acne, p = 0,00485 (p < 0,05), which means that green tea extract cream is better at fighting P. acne.
Co-Authors Abdilah, Galang Raka Adinatha, Ni Nyoman Maryaningtyas AMELIA, POPPY FRANSISCA Angelina, Dwi Agustin Anto Budiharjo Atina Hussaana Candrawuni Fatona, Riska Christina Menuk Sri Handayani Desy Rachmawati, Desy Dwi Kustriyanti Dyah Wulandari jaya rochkmana, meika Khoirul Fuad, Khoirul Mawardi, Reyhana Khansa Melasari, Ika Nopiyanti, Humairah Rizky Novita Rahmawati, Novita Prayogo, Fitra Adi Ramdani, Hendi Rismawati Rismawati Rismawati Siti Imroatul Maslikah Sopiatun, Mitri Syarafibi, Aulia Koen Taufiqurrachman Nasihun Vita, Vita Triani Adi Puteri Wiyadi, Ahmad Afa Haadiyan